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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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International Journal of Industrial Entomology
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Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 25, Issue 2 - Dec 2012
Volume 25, Issue 1 - Sep 2012
Volume 24, Issue 2 - Jun 2012
Volume 24, Issue 1 - Mar 2012
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Development of a Porous Scaffold-Manufacturing Method by Blending Silk Fibroin and Agarose Polymer Solutions
Park, Seung-Won ; Kweon, Hae-Yong ; Goo, Tae-Won ; Kim, Seong-Ryul ; Jo, You-Young ; Choi, Gwang-Ho ;
International Journal of Industrial Entomology, volume 25, issue 1, 2012, Pages 75~79
DOI : 10.7852/ijie.2012.25.1.075
Low-melting-temperature agarose gel solution, as a novel porogen was combined with a silk fibroin solution to generate interconnected porous networks. The porosity of the resultant silk fibroin-agarose scaffolds was greater than that of the scaffolds generated with agarose and deionized water. The porosities of silk fibroin scaffolds containing agarose gel at 0.5%, 1.0%, 1.5%, 2.0% [w/v] were 110.9%, 111.7%, 120.9%, and 123.0%, respectively. Lastly, the internal space generated in scaffolds after dissolution of the agarose gel provides a good environment for cell growth and movement within the scaffold.
Microsatellite Analysis of the Silkworm Strains (Bombyx mori) Originated from China
Kim, Kee-Young ; Kang, Pil-Don ; Ryu, Kang-Sun ; Kim, Ki-Hwan ; Sung, Gyoo-Byung ; Ji, Sang-Deok ; Kim, Mi-Ja ; Kim, Ik-Soo ;
International Journal of Industrial Entomology, volume 25, issue 1, 2012, Pages 81~92
DOI : 10.7852/ijie.2012.25.1.081
A total of 85 Chinese-origin silkworm strains preserved in Korea were genotyped for eight polymorphic micro-satellite loci. We obtained per-locus number of alleles, ranging from 5 to 14 with an average value of 9.5, perlocus observed heterozygosity, ranging from 0.07 to 0.99, and per-locus polymorphic information content (PIC), ranging from 0.34 to 0.82, indicating that some loci are highly variable. Phylogenetic analysis with the eight concatenated microsatellite loci showed no clustering on the basis of known strain characteristics. A total of 22 strain-specific apomorphic alleles, which discriminate 19 among 85 silkworm strains were obtained from eight loci. These strain-specific alleles, thus, can casually be utilized for the discrimination of applicable strains without any further typing of other loci. Furthermore, a substantial number of homozygote strains, represented by 27 among 76 alleles in eight loci were found. These results collectively suggest that the silkworm microsatellite DNA is actually and potentially important molecular markers for the eventual discrimination of silkworm strains that are preserved as hundreds in Korea.
Nutritional Value of Mealworm, Tenebrio molitor as Food Source
Ravzanaadii, Nergui ; Kim, Seong-Hyun ; Choi, Won-Ho ; Hong, Seong-Jin ; Kim, Nam-Jung ;
International Journal of Industrial Entomology, volume 25, issue 1, 2012, Pages 93~98
DOI : 10.7852/ijie.2012.25.1.093
Nutrition value of mealworm, Tenebrio molitor was analyzed due to increasing demand of usage as a protein source for domestic animals and even further for human consumption. The purpose of the present work was to determine the chemical compostion of the Tenebrio molitor larvae, adult that were maintained under standard condition for further usage of mass-rearing system and its exuvium, and excreta. Tenebrio molitor, larvae, adult, exuvium and excreta contained 46.44, 63.34, 32.87, and 18.51% protein respectively, suggested that even excreta could be used as an additional supplement in food recycling process. This protein was also rich in amino acids such as Isoleucine, leucine and Lysine which all met the nutritional value recommended by the Food and Agriculture Organization. Fatty acid composition was detected with high component of oleic acid (C18:1), along with linoleic acid (C18:2) and palmitic acid (C16) in all adult, larvae, exuvium and excreta. These oleic acid (C18:1), linoleic acid (C18:2) and palmitic acid (C16) components were the same or even highly contained in excreta of mealworm 22.29, 47.19 and 19.17% respectively. Longer chains of unsaturated fatty acids consisted of two to three double bonds are known as healthy product was recognized in large amount. These results show new ways to consume mealworms and its waste for animal and human consumption.
Comparison of the Genomic Structure of the Heat Shock Protein-88(Hsp88) Genes in the Four Entomopathogenic Fungal Strains, Paecilomyces tenuipes Jocheon-1, P. tenuipes, Cordyceps militaris, and C. pruinosa
Liu, Ya-Qi ; Park, Nam-Sook ; Kim, Yong-Gyun ; Kim, Keun-Ki ; Park, Hyun-Chul ; Son, Hong-Joo ; Lee, Sang-Mong ;
International Journal of Industrial Entomology, volume 25, issue 1, 2012, Pages 99~110
DOI : 10.7852/ijie.2012.25.1.099
Comparison on the genomic structure and phylogenetic relationship of the Hsp88 genes from P. tenuipes Jochoen-1, P. tenuipes, C. militaris and C. pruinosa was described. The Hsp88 genes from the three entomopathogenic strains, P. tenuipes Jocheon-1(strain), P. tenuipes(original species), and C. militaris contain the identical genomic structure, namely 5 introns and 6 exons with the length of 13, 62, 32, 1,438, 306, 288 nucleotides encoding 713 amino acid residues, whereas in case of C. pruinosa, it contains 4 introns and 5 exons with the length of 13, 62, 32, 1,744, 288 nucleotides encoding 713 amino acid residues. The genomic DNA length of the Hsp88 genes from P. tenuipes Jocheon-1 and P. tenuipes are both 2,600 nucleotides long in size. The Hsp88 genes from C. militaris and C. pruinosa are 2,582, 2,576 nucleotides long in size, respectively. Hsp88 genes of the P. tenuipes Jochoen-1, P. tenuipes, C. militaris and C. pruinosa also contain the conserved ATP-binding domain. Phylogenetic analysis of the Hsp genes of the four strains tested in this study showed that the fungal Hsp88 is divided into two separate clades, ascomycetes and deutromycete. Within the ascomycetes fungal clade, the P. tenuipes Jochoen-1 and P. tenuipes formed a subgroup, on the other hand, C. militaris and C. pruinosa formed another subgroup. Pair-wise comparison of P. tenuipes Jocheon-1 Hsp88 with those of P. tenuipes, C. militaris and C. pruinosa Hsp88s revealed significant identity in deduced amino acid sequence among these strains. The P. tenuipes Jocheon-1 Hsp88 showed 99% identity with the P. tenuipes, 97% identity with the C. militaris, and 98% identity with the C. pruinosa.
Hemocyte-specific Promoter for the Development of Transgenic Silkworm, Bombyx mori
Park, Seung-Won ; Goo, Tae-Won ; Kim, Seong-Ryul ; Choi, Gwang-Ho ;
International Journal of Industrial Entomology, volume 25, issue 1, 2012, Pages 111~114
DOI : 10.7852/ijie.2012.25.1.111
In previous studies we have shown that a sw17255 gene was expressed in hemocyte-specific tissues of the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae). It was verified that the sw17255 core promoter region contains elements that regulate the expression of this gene in hemocyte tissue; the selected promoter region spans nucleotides -1 to -2,112 upstream of the start codon. Each of the luciferase reporter gene expression vectors under the control of 4 different kinds of promoter candidates, (-2,112/-1), (-1,640/-1), (-1,169/-1) and (-579/-1), and the control reporter plasmid DNA, were introduced into B. mori larval coelom by direct injection using a syringe. The promoter candidate [E] (-579/-1) showed more than 1.67 fold transcriptional activity compared to control promoter activity. Higher productivity of an expressed gene in the transgenic silkworm by this promoter combination could be achieved in the near future. The foreign recombinant protein could be easily harvested from the blood of the transgenic silkworm.
Molecular Cloning and Characterization of Chymotrypsin Inhibitor and Chitin-Binding Protein Homologs from the Bumblebee Bombus terrestris
Qiu, Yuling ; Yoon, Hyung-Joo ; Jin, Byung-Rae ;
International Journal of Industrial Entomology, volume 25, issue 1, 2012, Pages 115~121
DOI : 10.7852/ijie.2012.25.1.115
The bumblebee Bombus terrestris is widely used in greenhouses to pollinate crops. Here, we report the molecular cloning and characterization of chymotrypsin inhibitor and chitin-binding protein homologs from B. terrestris. Two cDNAs encoding chymotrypsin inhibitor (Bt-CI) and chitin-binding protein (Bt-CBP) homologs were cloned from B. terrestris. Gene sequence analysis showed that Bt-CI gene consists of three exons encoding 75 amino acids, including a predicted 20-amino acid signal peptide, while Bt-CBP consists of two exons encoding 78 amino acids, including a predicted 26-amino acid signal peptide. The mature Bt-CI and Bt-CBP peptides contain ten and six conserved cysteine residues, respectively. Database searches using the deduced sequences of Bt-CI and Bt-CBP showed similarity to those from B. impatiens (96% peptide sequence identities). Bt-CI and Bt-CBP were expressed in both the venom gland and fat body of B. terrestris worker bees. The recombinant Bt-CI and Bt-CBP peptides were expressed in baculovirus-infected insect cells. Taken together, our findings describe the molecular characterization of Bt-CI and Bt-CBP from B. terrestris.
Foliar Application of Magnesium Sulphate and Basal Application of Calcium Carbonate: A New Dimension in Production of Tasar Crops
Sinha, Uma S.P. ; Das, Susmita ; Sinha, Manoj K. ;
International Journal of Industrial Entomology, volume 25, issue 1, 2012, Pages 123~127
DOI : 10.7852/ijie.2012.25.1.123
An experiment was carried out to boost the production of tasar crops through application of secondary nutrients. Different combinations of secondary nutrients were prepared and its effect was studied on the yield and quality of leaves of tasar food plant Terminalia tomentosa W & A with 2.4 m
2.4 m spacing and cocoon characters of tasar silkworm Antheraea mylitta Drury reared on them. Among different combinations of secondary nutrients, foliar application of magnesium sulphate (2%, w/v) and basal application of 3 quintal/ha of calcium carbonate (secondary nutrient combination SM5) was found to be the best in crop improvement. It improves the quantity and quality of leaves as well as the commercial characters of cocoons. As a result, silk production improves. Under this combination, leaf yield increased by 26.55% in comparison to control. Average increase in moisture, total mineral, crude protein and total carbohydrate was 3.26%, 20.84%, 15.39% and 17.85% respectively as compared with control. Further, bio assay studies revealed that average larval weight, E.R.R., cocoon weight, shell weight and silk ratio percent increased by 11.25%, 25.71%, 20.05%, 35.14% and 12.17% respectively over control which indicates that secondary nutrient combination
has significant role in improving the production of tasar crops.
Tetracycline-incorporated Silk Fibroin Films
Kim, Jong-Ho ; Shin, Bong-Seob ; Jeon, Jong-Young ; Kweon, Hae-Yong ; Jo, You-Young ; Lee, Heui-Sam ; Lee, Kwang-Gill ;
International Journal of Industrial Entomology, volume 25, issue 1, 2012, Pages 129~132
DOI : 10.7852/ijie.2012.25.1.129
Silk fibroin films incorporated with tetracycline was prepared and characterized by Fourier-transform infrared spectrometer and differential scanning calorimeter and examined antibacterial effect. The conformation of silk fibroin was changed from random coil to
sheet structure with incorporation of tetracycline. Antibacterial activity of the materials was evaluated against Gram-negative bacteria (Escherichia coli) and Gram-positive bacteria (Staphylococcus epidermis, and Staphylococcus aures) using agar plate method showing clear inhibition zone around tetracycline silk film. It is concluded that tetracycline-incorporated SF films are highly effective against bacteria.
Effect of Lyoprotectant on the Solubility and Structure of Silk Sericin
Kim, Moo-Kon ; Kwak, Hyo-Won ; Lee, Jeong-Yun ; Yun, Hae-Sung ; Kim, Min-Hwa ; Lee, Ki-Hoon ;
International Journal of Industrial Entomology, volume 25, issue 1, 2012, Pages 133~137
DOI : 10.7852/ijie.2012.25.1.133
To increase the solubility of lyophilized sericin, we added three types of lyoprotectant: sucrose, trehalose and dextran. The addition of lyoprotectant increased the solubility of lyophilized sericin especially when 1.0% of sucrose, 1.0% and 1.5% trehalose are added. The secondary structure of lyophilized sericin showed that the content of
-sheet or aggregated structure reduced in the presence of lyoprotectant. The morphology of lyophilized was also affected by the addition of lyoprotectant. Whereas flake structure was obtained in the case of pure sericin, a scattered and relatively small flake structure was formed in the presence of lyoprotectant. These finding shows that the presence of lyoprotectant prevents aggregation of sericin molecules and increases the solubility of lyophilized sericin.