Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
International Journal of Industrial Entomology
Journal Basic Information
Journal DOI :
Korean Society of Sericultural Science
Editor in Chief :
Volume & Issues
Volume 7, Issue 2 - Dec 2003
Volume 7, Issue 1 - Sep 2003
Volume 6, Issue 2 - Jun 2003
Volume 6, Issue 1 - Mar 2003
Selecting the target year
Soilborne Diseases of Mulberry and their Management
Sharma, D.D. ; Naik, V.Nishitha ; Chowdary, N.B. ; Mala, V.R. ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 93~106
Soilborne diseases pose a serious problem for mulberry cultivation during nursery plantation and established gardens, which cause severe loss in revenue generation of mulberry growers as compared to foliar diseases. Various soilborne diseases affect mulberry. Among them, root knot and root rot affect the established plantation resulting in severe loss in leaf yield apart from deterioration in leaf quality, which is a pre-requisite in successful sericulture to get the good quality of cocoons. Besides, stem-canker, cutting rot, collar rot and die-back, affect the initial establishment and survivability of mulberry plantation in nursery. The problem is difficult to handle, due to the complex nature of the diseases and also involvement of various biotic and abiotic factors. This is compounded by the occurrence of disease complex (especially nematode ＋ soilborne pathogenic microbes) in established mulberry gardens, which facilitates quick spread of the disease and enhance the plant mortality, resulting substantial loss in leaf yield. Therefore, prevention and timely control measures need to be taken up to protect the mulberry plants from different soilborne plant pathogens. In this review article, symptomatology, epidemiology, disease cycle and control measures of soilborne diseases of mulberry are discussed.
Sericulture Practices and Future Strategies under Present Scenario of Indian Subtropics
Singhal, B.K. ; Dhar, Anil ; Bindroo, B.B. ; Bakshi, R.L. ; Khan, M.A. ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 107~115
The present paper deals with the mulberry cultivation technology with package of practices for successful sericulture in Indian sub-tropics. The information on leaf nutritional quality in trained and untrained trees is provided. Besides, the current status of sericultural practices is discussed based on the problems faced by the industry and intensive field surveys undertaken in different areas of one of the most potential sericultural provinces.
Studies on Egg Production Index in Some Newly Evolved Multivoltine Breeds of the Silkworm, Bombyx mori L.
Gangopadhyay, Debnirmalya ; Singh, Ravindra ; Premalatha, V. ; Rao, D.Raghavendra ; Kariappa, B.K. ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 117~125
To know the impact of female pupal weight corresponding to the male cocoon shell weight upon fecundity, hatchability and percentage of non-viable eggs, the experimental multivoltine breeds namely BL67 and 96A were categorised into low, medium and high batches according to the weight of female pupae and male cocoon shells and moths emerged from low, medium and high female pupae were allowed to mate withmoths emerged from low, medium and high male cocoon shells. Both the experimental breeds of BL67 and 96A revealed a fairl trend in fecundity for high batch ＞ medium batch ＞ low batch. When data were statistically analysed, a significant (p ＜ 0.05) and a highly significant (p ＜ 0.01) increase in fecundity was observed in the medium and high batches of BL67 during November - December, 2002 whereas highly significant (p ＜ 0.01) increase was observed in the high batches of 96A during November - December, 2002. Data revealed a highly significant (p ＜ 0.01) increase in fecundity in medium and high batches of both the breeds during January - February, 2003. More interestingly, a significant (p ＜ 0.05) reduction was noted in percentage of non-viable eggs in high batches of 96A during January - February, 2003. But no significant variation in hatchability was noticed due to the effect of differential female pupal weight and male cocoon shell weight.
Baculovirus Expression and Biochemical Characterization of the Bombyx mori Protein Disulfide Isomerase (bPDI)
Goo, Tae-Won ; Yun, Eun-Young ; Kim, Sung-Wan ; Park, Kwang-Ho ; Hwang, Jae-Sam ; Kwon, O-Yu ; Kang, Seok-Woo ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 127~131
Protein disulfide isomerase (PDI) found in the endoplasmic reticulum (ER) catalyzes disulfide bond exchange and assists in protein folding of newly synthesized proteins. PDI also functions as a molecular chaperone and has been found to be associated with proteins in the ER. In addition, PDI functions as a subunit of two more complex enzyme systems: the prolyl-4-hydroxylase and the triacylglycerol transfer proteins. A cDNA that encodes protein disulfide isomerase was previously isolated from Bombyx mori (bPDI), in which open reading frame of 494 amino acids contained two PDI-typical thioredoxin active site of WCGHCK and an ER retention signal of the KDEL motif at its C-terminal, and we report its functional characterization here. This putative bPDI cDNA is expressed in insect Sf9 cells as a recombinant proteins using baculovirus expression vector system. The bPDI recombinant proteins are successfully recognized by antirat PDI antibody, and shown to be biologically active in vitro by mediating the oxidative refolding of reduced and scrambled RNase. This suggests that bPDI may play an important role in protein folding mechanism of insects.
A Bombyx mori Transcription Factor, ATFC Binds Directly to the UPRE of Molecular Chaperones
Goo, Tae-Won ; Yun, Eun-Young ; Kim, Sung-Wan ; Park, Kwang-Ho ; Hwang, Jae-Sam ; Kwon, O-Yu ; Kang, Seok-Woo ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 133~137
Cells respond to an accumulation of unfolded proteins in the endoplasmic reticulum (ER) by increasing transcription of genes encoding molecular chaperones and folding enzymes. The information is transmitted from the ER lumen to the nucleus by intracellular signaling pathway, called the unfolded protein response (UPR). In Saccharomyces cerevisiae, such induction is mediated by the cis-acting unfolded response element (UPRE) which has been thought to be recognized by Hac1p transcription factor. We cloned the ATFC gene showing similarity with Hac1p, and then examined to determine whether ATFC gene product specifically binds to UPRE by electrophoretic mobility shift assays. ATFC gene product displayed appreciable binding
UPRE. Therefore, we concluded that ATFC represents a major component of the putative transcription factor responsible for the UPR leading to the induction of ER-localized stress proteins.
Antiviral Potential of the Silkworm Deoxynojirimycin against Hepatitis B Virus
You, Jung-Eun ; Seong, Su-Il ; Kim, Young-Ho ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 139~144
Over 350 million people worldwide are chronic carriers of hepatitis B virus (HBV). Chronic viral infections of the liver can progress to cirrhosis, which may ultimately lead to hepatic failure or the development of hepatocellular carcinoma. There are two antiviral drugs on the market approved for clinical management of chronic HBV infections; interferon-alpha and the nucleoside analog lamivudine. However, they showed adverse side-effects. In the rational drug design for such therapies we would like to utilize antiviral drugs that inhibit the HBV replication in the liver. Investigation of natural extracts of silkworm exhibiting antiviral potential was held in the functional HBV polymerase activity and the release of virion particle in the HepG2.2.15 cell lines. HBV-producing transgenic mouse fed with silkworm DNJ molecule was shown as an inhibitor of serum HBV particles. We could represent this DNJ molecule as an antiviral potential complementing conventional therapies after preclinical tests against WHBV-infected animal model, woodchuck.
Molecular Cloning of a cDNA Encoding Putative Apolipophorin from the Silkworm, Bombyx mori
Yun, Eun-Young ; Goo, Tae-Won ; Kim, Sung-Wan ; Hwang, Jae-Sam ; Park, Kwang-Ho ; Kwon, O-Yu ; Kang, Seok-Woo ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 145~149
ApolipophorinIII (apoLp-III) is a protypical exchangeable apolipoprotein that is abundant in hemolymph of many insect species. Its function lies in the stabilization of low-density lipophorin particles (LDLp) crossing the hemocoel in phases of high energy consumption to deliver lipids from the fat body to the flight muscle cells. But, recent studies with naive Galleria mellonella-apoLp-III gave first indication of an unexpected role of that protein in insect immune activation. In this research, we cloned a cDNA encoding putative apoLp-III from the silkworm, Bombyx mori injected with E. coli and characterized its role. We constructed a cDNA library using whole bodies of B. mori larvae injected with E. coli, carried out the differential screening, and selected the up-regulated clones. Among these clones, we focused on a cDNA showing a high sequence similarity to the apolipophorinIII from other insects and analyzed the nucleotide and deduced amino acid sequences. The pupative B. mori Jam123 apoLp-III cDNA contained 1,131 bp encoding 186 amino acid residues. Phylogenetic analysis revealed that the nucleotide and amino acid sequences of the B. mori apoLp-III cDNA formed a highly inclusive subgroup with Bombycidae. But, it was interesting that B. mori Jam123 is closer to B. mandarina than B. mori P50 and B. mori N4. Northern blot analysis showed a signal in the fat body, posterior silkgland and midgut.
Effect of Photoperiodic Regimes on Oviposition and Colony Development of the Bumblebee, Bombus ignitus
Yoon, Hyung-Joo ; Kim, Sam-Eun ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 151~154
The effect of photoperiodic regimes on the oviposition and colony development of Bombus ignitus queens was examined with 0L, 8L, and 16L under
and 65% R. H. Among these photoperiod regimes, the oviposition rate at 8L and 16L was 80.2% and 83.1%, respectively, which was 12-15% higher than that at the dark condition (0L). Duration up to first oviposition at 8L and 16L was 17.5 days and 16.5 days, respectively, which was 2-3 days shorter than that at 0L. The colony foundation rate at 8L and 16L was 9.2% and 10.4%, respectively, which corresponded to 1.7-2.0-fold higher than the value at 0L. In addition, the rate of progeny-queen production at 8L and 16L was also two fold higher than that at 0L. Taken these together, the light conditions (8L and 16L) rather than dark condition (0L) were more suitable for oviposition and colony development for B. ignitus in the indoor rearing condition.
Genomic Structure of the Luciferase Gene of the Firefly, Lampyris noctiluca
Jianhong Li ; Park, Yong-Soo ; Lee, Sang-Chul ; Lee, Sang-Mong ; Kim, Jong-Gill ; Kim, Iksoo ; Kim, Keun-Young ; Sohn, Hung-Dae ; Jin, Byung-Rae ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 155~159
We describe here the complete nucleotide sequence and the exon-intron structure of the luciferase gene of the firefly, Lampyris noctiluca. The luciferase gene of the L. noctiluca firefly consisted of six introns and seven exons coding for 547 amino acid residues. From the translational start site to the end of last exon, the genomic DNA length of the L. noctiluca luciferase gene spans 1,976 bp.
Isolation and Purification of Polyhydroxylated Alkaloids from Silkworm (Bombyx mori L.)
Kim, Hyun-Su ; Ko, Hyun-Jung ; Cho, Yong-Seak ; Lee, Jae-Yeon ; Hwang, Kyo-Yeol ; Kim, Jin-Won ; Lee, Heui-Sam ; Kim, Iksoo ; Ryu, Kang-Sun ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 161~164
Several polyhydroxylated alkaloids were isolated from the extracts of freeze-dried silkworm powder, and purified by ion exchange chromatographic analysis. Through the HPLC analysis, we could identify 1-deoxynojirimycin (DNJ) and a kind of calystegin
(HS-58) as well as a noble compound (HS-74) from the purified polyhydroxylated alkaloids. In order to know the characteristics of these isolated alkaloids as enzyme inhibitors, glycosidase inhibition activities of these identified alkaloids including other two non-purified alkaloids (SWP 3-1 and SWP 3-2) were investigated.
Possible Presence of an Interleukin-6-Like Molecule in the Immunized Bombyx mori L. (Lepidoptera)
Kim, Iksoo ; Lee, Young-Shin ; Lee, Joon-Ha ; Kim, Sang-Hyun ; Kang, Pil-Don ; Lee, In-Hee ; Kim, Jin-Won ; Lee, Heui-Sam ; Kang, Seok-Woo ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 165~173
Cytokines represent an essential part of the innate immune response in mammals. Recently, several studies have reported the presence of cytokine-like activities and molecules in the invertebrates such as echinoderms, tunicates, mollusks and insects. In our serial study, we investigated presence of cytokines in the silkworm, Bombyx mori, infected with several immune inducers. Western blotting analysis using rabbit anti-human cytokines showed the presence of IL-6-like molecule in the hemolymph collected at 8 and 24 hrs after infection with peptidoglycan and oligodeoxynucleotide, and the molecular weight of the proteins was ∼45 kDa. We attempted to isolate the molecule by gel permeation HPLC, anion exchange chromatography, ultra centrifugation, and immuno-dot-blot assay, but until now the effort was not much successful yet. It, however, does not appear that the IL-6-like molecule in the silkworm larvae is a mere experimental artifact happened by Western blotting analysis. Instead, further experiment on this subject probably will provide us more fruitful result as detected in other invertebrates including insects.
Post Infection Physiobiochemical Alteration at Various Intensities of Leaf spot (Myrothecium roridum) in Mulberry
Kumar, P.M.Pratheesh ; Qadri, S.M.H. ; Pal, S.C. ; Mishra, A.K. ; Urs, S.Raje ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 175~180
Changes in biochemical constituents and physiological alteration were studied in various intensities (1-5%, 6-15%, 16-30%, 31-50% and ＞ 50%) of leaf spot (Myrothecium roridum) on mulberry leaves and compared with healthy leaves. Chlorophyll, total soluble sugar and total protein were decreased (P ＜ 0.01), but total phenol increased due to pathogen infection. Changes in biochemical constituents showed significant correlation with intensity of disease. Chlorophyll (
= 0.92), and protein ((
= 0.83) possessed negative while phenol ((
= 0.61) possessed positive correlation. Photosynthetic rate, transpiration rate, stomatal conductance, moisture content (%) and physiological water use efficiency (pWUE) were decreased, but stomatal resistance increased in the infected leaves. Physiological parameters also possessed significant (P ＜ 0.01) correlation with disease intensity. Photosynthetic rate ((
= 0.96), transpiration rate (
=0.88), stomatal conductance ((
= = 0.65), physiological water use efficiency ((
= 0.88) and moisture content (r = 0.85) were negatively but stomatal resistance ((
= 0.75) was positively correlated to disease intensities.
Genomic Structure of the Luciferase Gene and Phylogenetic Analysis of the Firefly, Pyrocoelia rufa
Jianhong Li ; Park, Yong-Soo ; Zhao Feng ; Kim, Iksoo ; Lee, Sang-Mong ; Kim, Jong-Gill ; Kim, Keun-Young ; Sohn, Hung-Dae ; Jin, Byung-Rae ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 181~189
We describe here the complete nucleotide sequence and the exon-intron structure of the luciferase gene of the firefly, Pyrocoelia rufa. The luciferase gene of the P. rufa firefly consisted of six introns and seven exons coding for 548 amino acid residues. From the translational start site to the end of last exon, however, the genomic DNA length of the P. rufa luciferase gene from the Korean and Chinese samples spans 1,968 bp and 1983 bp, respectively, and 3 amino acid residues were different to each other. Additionally, we also analyzed mitochondrial cytochrome oxidase I(COI) gene of the Chinese P. rufa fireflies. Analysis of DNA sequences from the mitochondrial COI protein-coding gene revealed 4 mitochondrial DNA sequence-based haplotypes with a maximum divergence of 0.7％. With the 20 P. rufa haplotypes found in Korea, phylogenetic analyses using PAUP and PHYLIP subdivided the P. rufa into three clades, termed clades A and B for the Korean sample, and clade C for the Chinese sample.
Effect of Antibiotic (Norfloxacin) Administration on Commercial Characters of New Bivoltine and Cross Breed Hybrid Silkworm (Bombyx mori L)
Rahmathulla, V.K. ; Nayak, Padmanav ; Vindya, G.S. ; Himantharaj, M.T. ; Rajan, R.K. ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 191~195
The impact of antibiotic (Norfloxacin) administration ,at different concentration (50 ppm and 100 ppm) on commercial characters of bivoltine
and the cross breed race
was studied. The result indicated that antibiotic administration with different concentrations significantly improved the rearing and cocoon parameters like larval duration, larval weight, growth index, single cocoon weight, single shell weight and shell ratio. The post cocoon parameters like average filament length, non-breakable filament length, raw silk percentage, raw silk recovery percentage, denier, reelability and neatness were recorded significantly higher in antibiotic treated batches. The better performances of these parameters were recorded with the increase of antibiotic concentration.
Comparative Study on Trichoplusia ni Tn 5B1-4 Cells and Bombyx mori BmN Cells for Recombinant Endostatin Production
Sohn, Bong-Hee ; Lee, Jong-Min ; Kang, Pil-Don ; Lee, Sang-Uk ; Kim, Yong-Soon ; Chung, In-Sik ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 197~201
The recombinant plasmids harboring a heterologous gene coding mouse endostatin were transfected and expressed stably in Trichoplusia ni Tn 5B1-4 cells and Bombyx mori BmN cells, respectively. Recombinant endostatin expressed in the stably transformed Tn 5B1-4 and BmN cells was secreted into the medium. BmN cells are relatively lower in maximum cell growth and recombinant endostatin production than Tn 5B 1-4 cells. Recombinant endostatin was also purified to homogeneity using a simple one-step
affinity fractionation method. Purified recombinant endostatin inhibited endothelial cell proliferation in a dose-dependent manner. The concentration at half-maximum inhibition
for recombinant endostatin was approximately 0.35
Relation among Food Consumption, Conversion and Cocoon Production in Silkworm, Bombyx Mori L. Reared at Different Temperatures
Muniraju, E. ; Shekharappa, B.M. ; Raghuraman, R. ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 203~208
Rearing temperature influences food consumption, conversion and cocoon production in silkworm Bombyx mori L. Young age
worms of Pure Mysore, a multivoltine were reared at 26, 28, 30 and
followed by combinations of these temperature levels during late age
. Food consumption, conversion, larval duration and growth for each instar were recorded. The influence of combinations of these temperature levels during rearing on survival and cocoon production was assessed. Food consumption increased with the temperature up to
instar and decreased during
instar. Temperature beyond
during young age followed by higher temperature (
and above) has shown deleterious effect on cocoon production. The paper discusses the rearing performance in relation to food intake and conversion under the tested temperature levels.
Studies on Manifestation of Hybrid Vigour in
and Three-Way Crosses of Multivoltine
Bivoltine Silkworm, Bombyx mori L.
Rao, D.Raghavendra ; Banerjee, Sharmista ; Kariappa, B.K. ; Singh, Ravindra ; Premalatha, V. ; Dandin, S.B. ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 209~219
An experiment was initiated to evaluate hybrid vigour in twelve F
hybrids and seven three-way crosses of multivoltine
bivoltine silkworm between newly evolved multivoltine breed BL67 with productive bivoltine CSR breeds and hybrids. Analysis of variances computed for different characters among F
hybrids and three-way crosses showed highly significant differences among them indicating presence of both additive and non-additive gene actions for the expression of these characters. Among twelve F
hybrids, two F
hybrids viz., BL67
have expressed significant heterosis for nine characters and two hybrids viz., BL67
for eight characters whereas out of nine three-way crosses, three hybrids viz., BL67
) and BL67
) expressed significant heterosis for eight characters. In the present study, it is observed that the cocoons of two hybrids viz., BL67
were found to be uniform as these hybrids showed lowest CV％ (5.35 and 5.38) among twelve F
hybrids and seven three way crosses between multivoltine
bivoltine hybrids. Four F
hybrids viz., BL67
) and BL67
) showed superiority in expressing hybrid vigour and are considered as best heterotic hybrids for commercial exploitation.ion.ation.ion.ation.ion.
Heterosis Studies in Some Elite Multivoltine Silkworm (Bombyx mori L.) Races with Popular Bivoltine N
Kumaresan, P. ; Sinha, R.K. ; Thangavelu, K. ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 221~229
Ten multivoltine female parental lines were crossed with popular bivoltine male silkworm breed NB
. Three types of heterosis parameters viz., heterosis over mid-parental value (hybrid vigour), heterobeltiosis (useful heterosis) and standard heterosis (standard check) were estimated for 15 economically important quantitative traits. The interaction among the hybrids and parents indicated significant effect for maximum characters. The heterotic effect of new hybrid combination was compared with popular hybrids viz., Pure Mysore
. Varied heterotic effect was observed for different traits for different hybrid combination. The results inferred that the crosses viz., BL
ranked top for 14 traits followed by Hosa Mysore
for 11 traits; PA
for 9 traits; BL
for 8 traits; Kolar Gold
for 7 traits; WAI
for 6 traits and MU
for 5 traits. Among these, the best hybrids Kolar Gold
were identified for longer filament length and fine denier. Similarly for higher cocoon yield and silk productivity BL
were found to be superior. These hybrid combinations are suitable for commercial exploitation at large scale.e.e.e.
The Physical Methods for Induction of Anti-Bacterial Substances in the Silkworm Larva, Bombyx mori
Gui, Zhongzheng ; Dai, Jianyi ; Zhuang, Dahuan ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 231~233
To understand the physical method for induction of anti-bacterial substances from the silkworm larvae, Bombyx mori, three physical methods, i.e., infrared radiation, ultraviolet radiation and ultrasonic wave, have been used in this study. The results have shown that ultrasonic wave can induce anti-bacterial sub-stances effectively than radiations in the B. mori larva. The induction of anti-bacterial substances was different from silkworm race to race. Summer-autumn silkworm race (Qiufeng
Baiyu) was easy to induce antibacterial substances. It is suggested that the ultrasonic wave is a simple and easy method for induction.
Mulberry Silkworm, Bombyx mori L., as a Host for Neurotoxic Braconidae I. Insect-toxic Properties of Bracon Venom Gland Extract and Its Fractions
Madyarov, S.R. ; Mirzaeva, G.S. ; Otarbaev, D.O. ; Khamidi, K.S. ; Kamilova, S.I. ; Akhmerov, R.N. ; Khamraev, A.S. ;
International Journal of Industrial Entomology, volume 7, issue 2, 2003, Pages 235~239
The silkworm Bombyx mori L. was studied as the potential “host” of popular in Uzbekistan biocontrol ectoparasite, entomophagous Bracon hebetor Say. Being one of representatives of economic-beneficial insects, the silkworm (larvae, pupae and imago) can be used as highly sensitive test organism for revealing of neuro toxic effects of insects venom as well as of their purified components in screening assays. In comparative aspect, except a mulberry silkworm, representatives of Uzbekistan pests cotton-boll worm Helycoverpa armigera Hbn., lesser mulberry pyralid Glyphodes pyloalis Wlk., codling moth Corpocapsa pomonella L., malaria mosquito Anopheles claviger and Colorado potato beetle Leptinotarsa decemlineata Say have been subjected to insect toxic test of bracon venom gland extract (VGE) and its fractions which were obtained by gel-chromatography on Sephadex G-100. The paralyzing effect of the VGE and its fractions was shown in a various degree on the pests.