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International Journal of Industrial Entomology
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Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 9, Issue 2 - Dec 2004
Volume 9, Issue 1 - Sep 2004
Volume 8, Issue 2 - Jun 2004
Volume 8, Issue 1 - Mar 2004
Selecting the target year
Conservation of Multivoltine Silkworm (Bombyx mori L.) Germplasm in India - An Overview
Kumaresan, P. ; Sinha, R.K. ; Mohan, B. ; Thangavelu, K. ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 1~13
Indian multivoltine mulberry silkworm (Bombyx mori L.) strains are poor in silk productivity and fibre quality. However, they are commercially exploited for the past five decades either in the form of pure breeds or cross breeds because of their adaptability to adverse climatic condition and acclimatization to specific regions and seasons. In the present paper conservation strategies of multivoltine silkworm genetic resources are dealt along with detailed information on accessibility of genetic resources, method of genetic resources conservation, characterization of genetic resources for morphological and productivity traits of economic importance. Selection of best ten accessions based on various economic parameters including yarn quality and their scope for future utilization are discussed.
Different Aspects of Mulberry Leaves Supplementation with Various Nutritional Compounds in Sericulture
Etebari, K. ; Kaliwal, B.B. ; Matindoost, L. ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 15~28
The supplementation of mulberry leaves, with the aim of yield, enhancement using vitamins, minerals and other compounds have generally been attended from 1990s and many researches have been conducted. More than 30 different compounds from complementary nutrients have been analyzed and in different regions where different climates various results have been achieved. This review has attempted to discuss the results of different works on enrichment of mulberry leaves with supplementary compound. Generally the most effects of these compounds are in the regions where productive parameters are low. In the areas that follow a normal pattern in economical parameters the enrichment of the leaves havent have significant economical effects.
Effect of Low Molecular Weight Silk Fibroin on the Inhibition of Tyrosinase Activity
Kang, Gyung Don ; Lee, Ki Hoon ; Shin, Bong Seob ; Nahm, Joong Hee ; Park, Young Hwan ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 29~33
Low molecular weight silk fibroin (LMSF), which was prepared by hydrolysis of silk fibroin using high-temperature and high-pressure method, was found to inhibit the oxidation of L-3,4,-dihydroxyphenylalanine (L-DOPA) catalyzed by mushroom tyrosinase (EC 188.8.131.52). LMSF contained mostly free amino acids such as L-glycine, L-alanine, and L-serine and oligopeptides, mainly glycine-alanine dimer. As a result of analyzing the inhibition kinetics from Lineweaver-Burk plots, L-glycine and glycine-alanine dimer showed noncompetitive behavior while uncompetitive behavior was observed in L-alanine, and L-serine. When weight percent concentration of
was compared, L-glycine was most effective on the inhibition and LMSF was also good enough for the inhibition effect of tyrosinase activity. LMSF showed a mixed-type inhibition and the inhibitory mechanism of LMSF might be caused by free amino acids and oligopeptides. As a result of spectroscopic observation with time, initial rate of increase of DOPAchrome decreased remarkably and the time to reach maximum absorbance increased as an increase of the concentration of L-glycine, meaning that L-glycine made itself mainly responsible for the formation of chelate with
in tyrosinase. However, in case of L-alanine, L-serine, and especially glycine-alanine dimmer, the production of DOPAchrome after an arrival at maximum absorbance decreased, indicating the production of adducts through the reaction with DOPAquinone.
Resistance to Bombyx mori Densonucleosis Virus Type 1 and Its Inheritance in Silkworm, Bombyx mori L.
Sen, Ratna ; Nataraju, B. ; Balavenkatasubbaiah, M. ; Premalatha, V. ; Thiagarajan, V. ; Datta, R.K. ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 35~40
Bombyx mori densonucleosis virus type 1 (BmDNV1)- a non occluded virus causes flacherie disease in the susceptible stocks of the silkworm, Bombyx mori. However, some stocks are non-susceptible. Non-susceptibility to BmDNV1 in B. mori is a unique case where the virus infection is completely inhibited by a single gene of the host. A survey conducted by this institute in some parts of Karnataka state has revealed that, 43.05% of the total incidence of flacherie disease caused by non-occluded viruses, are due to the synergistic infection of B. mori densonucleosis and infectious flacherie virus. Earlier study indicated that rearing of BmDNV1 resistant silkworm stock is effective in protecting silkworm against BmIFV also. In the present study the response of 78 silkworm stocks which include 42 of non-diapausing and 36 of diapausing groups, to BmDNV1 is investigated. Newly ecdysed third instar larvae were inoculated per-os with 10% inoculum of BmDNV1 extracted from the mid-gut of infected silkworm. One non-diapausing and three diapausing silkworm stocks were found to be resistant to BmDNV1. Eleven silkworm stocks were found to possess moderate resistance whereas rest sixty three were found to be susceptible to BmDNV1. Genetic analysis has shown that the resistance to BmDNV1 is autosomally inherited and controlled by a major dominant or a major recessive gene in different silkworm stocks. These resistant stocks can be utilized as the resource material to develop BmDNV1 resistant commercial hybrids. The selection strategies, depending upon the mode of inheritance of resistance in the resource material chosen, are discussed.
Development of a Polyvoltine Breed -
(Pg) of the Silkworm, Bombyx mori L. with Parthenogenetic Origin
Singh, Ravindra ; Rao, D.Raghavendra ; Gangopadhyay, Debnirmalya ; Choudhary, Nazia ; Kariappa, B.K. ; Dandin, S.B. ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 41~46
A breeding programme was initiated by utilizing a robust bivoltine breed CSR
and a polyvoltine breed Cambodge with the main objective of developing robust polyvoltine silkworm breeds/hybrids. At F
, parthenogenetic development was induced following warm water treatment of eggs at 46
for 18 min followed by two backcrosses with Bl
, an evolved polyvoltine breed. The newly developed breed was subjected for hybrid study using eight hybrid combinations in the laboratory at F
hybrids between newly developed breed Bl
(Pg) and promising bivoltine breeds exhibited their superiority by expressing significant hybrid vigour for several economic characters like cocoon yield/10,000 larvae, cocoon weight, cocoon shell weight, cocoon shell ratio and denier. Study on cocoon shape variability revealed that cocoons of all the F
hybrids except BL
were found comparatively uniform in shape.pe.
Evaluation of New Commercial
Hybrids of Silkworm (Bombyx mori L.) with Participation of Sex-limited Lines
Petkov, N. ; Petkov, Z. ; Grekov, D. ; Arnaudova, K. ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 47~51
To evolve silkworm hybrids with higher survival and productivity and easy and effective seed cocoon production, three new evolved bivoltine hybrids of silkworm (Bombyx mori L.), created with participation of sex-limited lines at eggs and larva stage were evaluated with control Super
hybrid at Plovdiv Agrarian University during 2000 - 2002. ANOVA analysis was employed and the performance of hybrids was observed in respect of main quantitative traits. The results obtained show significant superiority of new hybrids T
6/ and their reciprocal crosses over the control. They were characterized with 99% hatchability of eggs, 93-95% silkworm survival, 2.32- 2.42 g cocoon weight, 53.5-55.7 cg shell weight, 22.9-23.1% shell ratio, 1,307-1,326 m filament length, 3.08-3.17 denier, 95-96% reelability, 44.4-45.1% silk ratio, 569-593 number of normal eggs per lying and 347-364 mg weight of normal eggs per lying. Newly evolved hybrids were manifested high productivity, 41.0-43.6 kg cocoon yield and 7.33-7.78 kg raw silk yield per one box (20,000
200 viable eggs), which significant surpass the control Super
hybrid with 7.6-14.4% and 10.8-17.6%, respectively. T
24/ and XT
2/ were considered as highly productive hybrids to local conditions and found suitable to rear in spring season.n.
Heterotic Expression in Silk Productivity of Newly Evolved Thermo-tolerant Bivoltine Hybrids of Silkworm Bombyx mori L.
Rao, P.Sudhakara ; Datta, R.K. ; Vijaya Kumari, K.M. ; Palit, A.K. ; Haque Rufaie, S.Z. ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 53~58
Studies on heterotic expression in the silk productivity of 66 thermo-tolerant bivoltine hybrids reared under high temperature
and low humidity (60
5%) conditions revealed that manifestation of heterosis was highly significant for majority of the quantitative traits contributing to more cocoon productivity. Observations were made on 8 economically important traits such as fecundity, pupation rate, cocoon yield, cocoon weight, cocoon shell weight, cocoon shell ratio, cocoon filament length and raw silk. Analysis of variance results revealed great deal of variability indicating significant (P < 0.001) variations. Out of 66 hybrids, 7 hybrids exhibited positive heterosis for seven traits and 4 hybrids expressed significant hetero-beltiosis for 6 traits, out of 8 traits evaluated. Based on results, the hybrids
are adjudged as most promising ones and can be exploited commercially in tropical climates to increase the cocoon productivity.
Electrophoretic Analysis of Nonspecific Esterases in Silkworm (Bombyx mori L.) Female Genital Organs and Eggs
Staykova, Teodora ; Grekov, Dimitar ; Panayotov, Mihail ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 59~63
By using PAGE, a study was made on the nonspecific esterase spectra of female genital organs and eggs in Bombyx mori L. The expression of 11 esterase bands was detected during ontogenesis of races and inter-races hybrids kept in Bulgaria. The gene activity of 9 esterase loci was assumed. Esterases specific for the spectrum of diapausing eggs were observed. In two esterase zones, intra- and inter-breed polymorphism was found. Based on the same breed specific expression, the existence of correspondence between esterase bands from spectra of different silkworm tissues and organs was suggested. Stage-specific expression of esterases in female genital glands, indicative of differentiated gene activity during ontogenesis, was established.
Molecular Cloning and mRNA Expression a Glutathione S-Transferase cDNA from the Spider, Araneus ventricosus
Shin, Geun Ho ; Kim, Hyung Suk ; Kwon, Dong Wook ; Lee, Jin Young ; Byeon, Gyeong Min ; Sohn, Hung Dae ; Jin, Byung Rae ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 65~71
A fat body-specific glutathione S-transferase cDMA was cloned from the spider, Araneus ventricosus. The cDNA encoding A. ventricosus glutathione S-transferase (AvGST) is 645 base pairs long with an open reading frame of 215 amino acid residues with a calculated molecular weight of approximately 24 kDa. Northern blot analysis showed the tissue-specifically expression of AvGST in the A. ventricosus fat body.
Impact of Low Concentration Fluoride on Toxification and Biological Traits of Silkworm, Bombyx mori
Hosagoudar, S.R. ; Yuin, Chen ; Yuanxiang, Jin ; Manjunatha, H.B. ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 73~78
The impact of low concentration fluoride on toxification was studied by treating
instar silkworm larvae of Qiufeng
Baiyu (Fluoride resistant strain) and Jinshong
Haoyue (Fluoride sensitive strain). The
instar silkworm larvae of Zhenong-l, Shu-12, Qiufeng
Qhiushi, Jinshong and Haoyue were fed mulberry leaves treated with sodium fluoride at different concentrations to determine its effect on fecundity. Harmful effects of low concentration fluoride at early instars on growth, development and economic characters of Bombyx mori are related to its resistance. Harmful effect on resistant strain Qiufeng
Baiyu manifested on prolongation of instar duration of 15 and 6% in 70 ppm, where as the sensitive breed Jinshong
Haoyue manifested with 100% mortality in 50 ppm. The impact of low concentration fluoride on fecundity resulted in considerable increase in formed eggs (9%) and laid eggs (9%) in Zhenong-l and Shu-12 among treated population over control. The present observations emphasis that fluoride apart from exhibiting harmful effects on silkworm growth and development, however, its low concentration do playa vital role in inducing some positive effect on fecundity and cocoon traits.
Studies on Long-term Preservation of Eggs of Indian Tropical Multivoltine Silkworm (Bombyx mori L.) Genetic Resources
Kumaresan, P. ; Thangavelu, K. ; Sinha, R.K. ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 79~87
The silkworm rearing and growth parameters of 63 multivoltine silkworm accessions under extended period of egg preservation at 5
from 30 days to 45 days were studied. The results indicate that, nine accessions did not respond to extended period of egg preservation at low temperature and the remaining 54 accessions responded to the treatment and three rearings were conducted for comparision with the control; to estimate the effect of prolonged egg preservation at low temperature. The non-parametric tests statistics (Wilcoxon tests) was adopted for comparing the mean performance of treated batches (45 days) over the control (30 days). Highly significant variability was found among the accessions for all the parameters under study. The genetically controlled morphological characters were not altered in the treated batches, which were found to be on par with that of control. However, the total larval duration varied significantly over the control in 51 accessions. Similarly, the fifth age larval duration of 27 accessions showed decreasing trend compared to control. Altogether 41 accessions were found to be tolerant to long-term cold preservation upto 45 days, without showing any significant variation for morphological as well as essential quantitative traits. These accessions may be recommended for long-term egg preservation schedule up to 45 days, which will reduce the cost of conservation of these silkworm germplasm.
Breeding of a New Silkworm Variety, Kumhwangjam, with a Sex-Limited Cocoon Color for Spring Rearing Season
Kang, Pil Don ; Sohn, Bong Hee ; Lee, Sang Uk ; Kim, Mi Ja ; Jung, I Yeon ; Kim, Young Soon ; Kim, Yung Dae ; Lee, Heui Sam ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 89~93
A new silkworm variety, Kumhwangjam, with a sex-limited cocoon color for spring rearing season is F
hybrid between Jam 309, a Japanese race bred from Y1/9153, and Jam 310, a Chinese race from Y1/8586. In the local adaptability test performed at 8 local areas in spring rearing season of 2003, major characteristics of Kumhwangjam were mainly similar to those of the check variety Kumokjam. The new silkworm variety, Kumhwangjam, showed a sex-limited cocoon color: yellow for female and white for male with elliptical cocoon shape. This sex-limited cocoon color can be used as a valuable marker for discrimination between female and male for various purposes.
Correlation between Body Weight and Colony Development of the Bumblebee Queen, Bombus ignitus
Yoon, Hyung Joo ; Kim, Sam Eun ; Lee, Sang Beom ; Park, In Gyun ; Seol, Kwang Youl ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 95~99
This study was carried out to investigate the effect of body weight of queen on the developmental characteristics of foundation queens in the Bombus ignitus collected in Korean locality. Queens were classified into six groups based on their body weight (i.e., 0.4 g, 0.5 g, 0.6 g, 0.7 g, 0.8 g and 0.9 g). The average weight of 200 foundation queens collected was 0.657
0.095 g and 0.6 g-class was most abundant (38 %) among six weight classes. The queen that had the heavy body weight showed the trends of higher rate of oviposition, colony foundation and progeny-queen production and shorter periods of preoviposition and colony foundation, suggesting the positive correlation between the body weight of queen and colony developmental characteristics. Also, the numbers of worker and progeny-queen emerged from the queen with heavy body weight were slightly higher than those with light body weight queen.
Preliminary Report on Breeding of Coarse Size Variety
of Bombyx mori L
Zhao, Yuan ; Wu, Yangchun ; Qian, Heying ; He, Yiyuan ; He, Simei ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 101~105
A number of Chinese, Japanese and European silkworm strains with coarse size filament were used for breeding the coarse size variety. Through cross breeding combined with pedigree selection within thirteen generations, a pair of coarse size silkworm variety, named
, was obtained after five years. Laboratory trials showed that the filament size of its
hybrid was over 4.3 D, and the other economic characters were also good.
Prevention of Diapause in Bivoltine Eggs of the Silkworm, Bombyx mori, L., through a Cross with the Race KS-10 as Male Parent
Mundkur, Rajendra ; Murthy, Mallesha ; Mahadevappa ; Raghuraman, R. ; Bongale, U.D. ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 107~109
The present investigation reports a phenomenon hitherto unknown in tropical sericulture, wherein dia-pause nature of bivoltine eggs is overcome through a cross with a non-diapausing race of silkworm, Bombyx mori, L. Eggs of bivoltine silkworm Bombyx mori, L. generally do not hatch under tropical conditions. To prevent diapause, they are subjected to acid treatment or low temperature hibernation scheduled. A race developed at KSSRDI is found to prevent the diapause nature of bivoltine eggs when crossed as male parent, without any acid treatment or hibernation schedule. This phenomenon is reported for the first time, being unique, opens up interesting area of research in silkworm genetics of commercial implications in the industry.
Nutritional Efficiency in Antheraea mylitta D. during Food Deprivation
Rath, S.S. ; Sinha, B.R.R.P. ; Thangavelu, K. ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 111~115
Nutrition plays an important role in maintaining the larval health, cocoon quality and reproductive potential in Antheraea mylitta D. Nutritional efficiency greatly influenced if food is not adequate and of quality. A. mylitta silkworms were subjected to food deprivation for the period of 0 hr to 12 hrs /day to assess its effect on various nutritional parameters and indices, and its manifestation at different levels. Food ingesta, digesta, gain in body weight declined significantly at each level of deprivation, so also food utilization efficiency like consumption index (CI), growth rate (GR), approximate digestibility (AD), and efficiency of conversion of ingested food (ECI). This stress leads to decline in mean daily food ingesta by 16.73% to 39.76% and digesta by 28.98% to 54.01 % following a significant reduction in average daily body weight gain (27.68% to 55.09%). Food deprivation a1so caused significant loss in the silk gland weight, cocoon and shell weight (14.37% to 53.69%), lowered the fecundity (35.86 % to 83.59%) and in number of eggs laid per gram body weight, but simultaneously the number of non-chorionated eggs increased significantly.
Analysis of Diapause-Associated Changes in Silkworm Egg Proteins
Go, Hyun-Jeong ; Hwang, Jae-Sam ; Kim, Young-Tae ; Kim, Hyun-Su ; Kang, Seok-Woo ; Chang, Jong-Su ; Lee, Sang-Mong ; Lee, Bong-Hee ; Seong, Su-Il ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 117~121
The patterns of diapause-associated proteins of silkworm eggs were analyzed by two-dimensional (2-D) gel electrophoresis. Among the hundreds of spots on the 2-D gels, at least two proteins were considered to be associated with diapause. A protein, spot 4, with an approximate molecular weight of 38 kDa and pI 6.1 was observed in the HCI-treated, cold-treated, and diapause eggs, respectively. Spot 4 was undetectable in unfertilized eggs and non-diapause eggs at two days after oviposition, suggesting that this protein may be associated with the entrance to diapause. A protein, spot 11, with an approximate molecular weight of 21 kDa and pI of 61 was detected in the unfertilized, HCl-treated, and cold-treated eggs, respectively, after oviposition by normal moths. In diapausing eggs, a protein corresponding to spot 11 was observed in 3-, 5-, and 30-day-old eggs, while the protein was not detected one-day-old eggs. The protein corresponding to spot 11 was not detected in unfertilized and non-diapause eggs obtained from subesophabeal ganglion (SG)-extirpated moths either. Spot 11 was also considered to be a diapause specific protein, which occurred at only early embryonic stage under the control of diapause-downregulated gene.
Molecular Cloning of a Profilin cDNA from Bombyx mori
Wei, Yadong ; Gui, Zhongzheng ; Choi, Young Soo ; Guo, Xijie ; Zhang, Guozheng ; Sohn, Hung Dae ; Jin, Byung Rae ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 123~126
The actin-binding protein profilin cDNA was firstly isolated from the lepidopteran insect, silkworm Bombyx mori. The B. mori profilin cDNA contains an open reading frame of 378 bp encoding 126 amino acid residues and possesses three cysteine residues. The deduced amino acid sequence of the B. mori profilin cDNA showed 80% identity to Apis mellifera profilin and 72% to Drosophila melanogaster profilin. Northern blot analysis showed that B. mori profilin is highly expressed in epidermis and less strongly in silk gland. In addition, Northern blot analysis revealed the presence of B. mori profilin transcripts in all tissues examined, suggesting that B. mori profilin gene is expressed in most, if not all, body tissues.
Molecular Cloning of the Myosin Light Chain-2 cDNA of Gryllotalpa orientalis
Cha, So Young ; Hwang, Jae Sam ; Kim, Iksoo ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 127~130
We describe here the cloning and characterization of a cDNA encoding a putative myosin light chain-2 (MLC-2) from the mole cricket, Gryllotalpa orientalis. The G. orientalis MLC-2 cDNA sequences comprised of 615 bp with 205 amino acid residues with a calculated molecular weight of approximately 23 kDa. The deduced protein sequence of G. orientalis MLC-2 cDNA showed 64% and 54% identity to Drosophila melanogaster MLC-2 and D. yakuba MLC-2, respectively. Northern blot analysis confirmed the muscle-specific expression of G. orientalis MLC-2.
Influence of the Mineral Potassium Permanganate on the Biochemical Constituents in the Fat Body and Haemolymph of the Silkworm, Bombyx mori L.
Bhattacharya, A. ; Kaliwal, B.B. ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 131~135
Oral supplementation with potassium permanganate (30, 50 and 100
) to fifth instar larvae of the
race of the silkworm, B. mori resulted in a significant increase in the glycogen content of the fat body and haemolymph trehalose. The protein content of the fat body is also significantly increased in all the potassium permanganate treated groups where as that of the haemolymph is significantly increased only in the 30
fed group. The total lipids content of the fat body increased significantly in all the potassium permanganate treated groups. This indicates that the potassium permanganate may stimulate metabolic activity, there by influencing the biochemical contents in the fat body and haemolymph of the silkworm, B. mori.
Genetic Analysis of the Diamondback Moth, Plutella xylostella, Collected from China Using Mitochondrial COI Gene Sequence
Li, Jianhong ; Choi, Yong Soo ; Kim, Iksoo ; Sohn, Hung Dae ; Jin, Byung Rae ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 137~144
The diamondback moth, Plutella xylostella, is notorious because of its extensive potential and actual dispersal ability. Previously, the Korean populations of P. xylostella was extensively collected and analyzed for their genetic population structure using a portion of mitochondrial DNA (mtDNA). One of the postulated characteristics on population genetic structure of the species includes the presence of heterogeneous haplotypes, possibly possessed by some dispersed ones from neighboring countries. In this study, we sequenced ten P. xylostella collected from China (∼2,000 km away from the middle part of Korea) to know the genetic relationships of these to the Korean P. xylostella. Sequence analysis of the identical portion of COI gene resulted in five haplotypes with the sequence divergence ranging from 0.5% (two nucleotides) to 1.1 % (five nucleotides) among them and from 0.7% (three) to 2.5% (11) to the pre-existing 52 Korean haplotypes. Phylogenetic analysis showed that the Chinese P. xylostella were neither clearly separated from the Korean haplotypes nor clustered with one heterogeneous Korean haplotype. This result reinforces the significance of gene flow in this species and suggests to exclude the possibility that the heterogeneous Korean haplotypes may have emigrated from China, where our samples were obtained, although further extensive investigation is required.
Construction of the Full-length cDNA Library and Selection of Diapause-Associated cDNA Clones from Bombyx mori Diapausing Eggs and Diapause-Activated Eggs
Hwnag, Jae-Sam ; Go, Hyun-Jeong ; Goo, Tae-Won ; Yun, Eun-Young ; Choi, Kwang-Ho ; Seong, Su-Il ; Kim, Sung-Kuk ; Lee, Sang-Mong ; Kim, Iksoo ; Kim, Nam-Soon ; Kang, Seok-Woo ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 145~148
As an initial step to define the molecular mechanism of diapause during embryogenesis of the silkworm, Bombyx mori, mRNA transcripts from diapausing eggs and diapause-activated eggs were compared by differential expression using cDNA microarray. Twenty-four individual cDNA clones were identified. Amomg them, ten genes including alcohol dehydrogenase, dead box-l, cytochrome oxidase subunit I and 18 wheeler showed increased expression in the diapause-activated eggs. The rest of fourteen genes showed increased expression in diapausing eggs.
Molecular Cloning of a LIM Protein cDNA from the Mulberry Longicorn Beetle, Apriona germari
Gui, Zhongzheng ; Wei, Yadong ; Yoon, Hyung Joo ; Kim, Iksoo ; Guo, Xijie ; Jin, Byung Rae ; Sohn, Hung Dae ;
International Journal of Industrial Entomology, volume 9, issue 1, 2004, Pages 149~153
Here we report the molecular cloning of a LIM protein cDNA of the CRP (cysteine-rich protein) family from the mulberry longicorn beetle, Apriona, geramri. The A. germari LIM protein cDNA contains an open reading frame of 276 bp encoding 92 amino acid residues with a calculated molecular weight of approximately 10 kDa. The A. germari LIM protein contains the cysteine-rich consensus sequence of LIM domain and the glycine-rich consensus sequence observed in cysteine-rich protein family 1 (CRP1). The potential nuclear targeting signal is retained. The deduced amino acid sequence of the A. germari LIM protein cDNA showed 81 % identity to both Bombyx mori muscle LIM protein (Mlp) and Drosophila melanogaster Mlp60A and 77% to Epiblema scudderiana Mlp. Northern blot analysis showed that A. germari LIM protein is highly expressed in epidermis and muscle, and less strongly in midgut, but not in the fat body.