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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Microbiology and Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 1, Issue 4 - Dec 1991
Volume 1, Issue 3 - Oct 1991
Volume 1, Issue 2 - Aug 1991
Volume 1, Issue 1 - Mar 1991
Selecting the target year
Enhancement of Ethanol Tolerance of Lactose Assimilating Yeast Strain by Protoplast Fusion
Ryu, Yeon-Woo ; Jang, Heang-Wook ; Lee, Haing-Sook ;
Journal of Microbiology and Biotechnology, volume 1, issue 3, 1991, Pages 151~156
In order to construct a yeast strain having high ethanol tolerance together with good lactose fermentation ability, the protoplast fusion using Saccharomyces cerevisiae STV 89 and Kluyveromyces fragilis CBS 397 was carried out. Auxotrophic mutants of K. fragilis were obtained as a selection marker by treatment of ethylmethane sulfonate. The best mutant for protoplast fusion was selected based on the capabilities of
production and lactose fermentation. The protoplast fusion using polyethylene glycol and calcium chloride solution led to the fusion frequence of
and a number of fusants were obtained. Among these fusants, a fusant F-3-19 showed the best results in terms of ethanol tolerance,
activity and lactose fermentation. The performance of lactose fermentation and ethanol tolerance by this fusant were better than those of K. fragilis. Study on the ethanol tolerance having relation to fatty acid composition and intracellular ethanol concentration revealed that the fusant F-3-19 had a higher unsaturated fatty acids content and accumulated less amount of intracellular ethanol compared with a parent of K. fragilis.
Amplification of Glutathione Production in E. coli Cells Using Recombinant DNA Techniques
Nam, Yong-Suk ; Park, Young-In ; Lee, Se-Yong ;
Journal of Microbiology and Biotechnology, volume 1, issue 3, 1991, Pages 157~162
Conditions for glutathione production in E. coli cells which possess pGH501 (2 gshI+gshII) were studied. In terms of ATP supply for the glutathione synthesis, two different systems have been constructed and compared. When the acetate kinase reaction of E. coli was used for ATP generation, 20 mM of L-cysteine was completely converted to glutathione by toluene-treated E. coli cells (100 mg/ml) harboring pGH501 within 2 h at
. However, considering the economical aspects, the glycolytic pathway of yeast was chosen as a better system for ATP generation. The optimal concentrations of reactants for glutathione production were determined to be as follows; 80 mM L-glutamate, 20 mM L-cysteine, 20 mM glycine, 20 mM
, 50 mM potassium phosphate buffer (pH 7.5), 400 mM glucose, polyoxyethylene stearylamine (
), toluene-treated E. coli HB101/pGH501 (100 mg/ml), and dried yeast cells (400 mg/ml). The conversion ratio of L-cysteine to glutathione was 80% (about 5 mg/ml) under optimal condition within 6 h at
A New Potent Angiogenesis Inhibitor, FR-118487
Otsuka, Takanao ; Ohkawa, Takehiko ; Shibata, Toshihiro ; Oku, Teruo ; Okuhara, Masakuni ; Terano, Hiroshi ; Kohsaka, Masanobu ; Imanaka, Hiroshi ;
Journal of Microbiology and Biotechnology, volume 1, issue 3, 1991, Pages 163~168
A new angiogenesis inhibitor, FR-118487 was obtained by chemical modification of FR-111142 which was isolated from the fermentation products of Scolecobasidium arenarium F-2015. The antiangiogenic activity of FR-118487 was compared with that of the parent compound, FR-111142. In the endothelial cell proliferation test in vitro and the angiogenesis in the chick embryo chorioal-lantoic membrane assay, FR-118487 had about 5∼10 times stronger antiangiogenic activities than FR-111142. In addition, FR-118487 inhibited the angiogenesis in the rabbit corneal assay and suppressed the solid tumor growth in mice. These findings showed that FR-118487 would be a unique antiangiogenic agent with promising antitumor activity.
Plasmid Linkage of Bacteriocin Production and Sucrose Fermentation Phenotypes in Pediococcus acidilactici M
Kim, Wang-June ; Ha, Duk-Mo ;
Journal of Microbiology and Biotechnology, volume 1, issue 3, 1991, Pages 169~175
Pediococcus acidilactici strain M produced a bacteriocin which was proteinaceous, heat stable, and exhibited antimicrobial activity against lactic acid bacteria, variety of food spoilage and pathogenic bacteria. The antimicrobial activity was not caused by
and organic acid, and was remained between pHs of 4.0 to 9. Molecular weight of crude bacteriocin was approximately 2, 500. Phenotypic assignment after plasmid cruing experiment demonstrated that a 53.7 kilobase (kb) plasmid, designated as pSUC53, was responsible for the sucrose fermentation phenotype (
) and a 11.1 kb plasmid, designated as pBAC11, was associated with bacteriocin production phenotype (
). Neither of the two plasmids were linked to antibiotic resistance.
Properties of Dextransucrase from Leuconostoc mesenteroides Isolated from Sikhae
Rhee, Suk-Hyung ; Lee, Cheal-Ho ;
Journal of Microbiology and Biotechnology, volume 1, issue 3, 1991, Pages 176~181
Studies on the optimum conditions for dextran production and the properties of dextransucrase (DS) were performed with Leuconostoc mesenteroides from Sikhae and Leuconostoc mesenteroides NRRL B-512(F). Dextransucrases were partially purified by lyophilization of the culture supernatant and subsequent gel chromatography on Bio-Gel A-5(m). The storage stabilities of Sikhae DS and B-512(F) DS were decreaed by the addition of dextranase. The optimum conditions for the enzyme stability were pH 5 and below
. The B-512(F) DS lost the activity at pH 4, while Sikhae DS had 30% of the activity at the same pH. The activity of DS was decreased by EDTA. confirming the metalloprotein character of the enzymes, and was restored by the addition of calcium ions. Concanavalin A completely removed the activity of DSs, confirming the glycoprotein character of the enzymes.
Effects of Acrylonitrile and Acrylamide on Nitrile Hydratase Action of Brevibacterium sp. CH1 and CH2
Lee, Cheo-Young ; Hwang, Jun-Sik ; Chang, Ho-Nam ;
Journal of Microbiology and Biotechnology, volume 1, issue 3, 1991, Pages 182~187
The effects of acrylonitrile and acrylamide on the enzyme action of nitrile hydratase of Brevibacterium sp. CH1 and CH2 strains used for the biotransformations of nitriles were studied. The excessive substrate (acrylonitrile) and product (acrylamide) inhibited the enzyme activity competitively. In comparison with 0.2 mol/l of CH1 strain, the substrate inhibition of CH2 strain began to appear only at a high acrylonitrile concentration of 0.91 mol/l. In a packed bed reactor, dispersed plug flow model was proposed and this model was proved to be valid by the experiment. Also acrylamide productivity decreased sharply when acrylamide concentration in the substrate solution exceeded 20％ (wt/v).
Purification and Characterization of
from Penicillium verruculosum
Chun, Soon-Bai ; Kim, Dong-Ho ; Kim, Kang-Hwa ; Chung, Ki-Chul ;
Journal of Microbiology and Biotechnology, volume 1, issue 3, 1991, Pages 188~196
was purified to homogeneity from the culture filtrate of P. verruculosum by column chromatography. The enzyme was a glycoprotein with a relative size of approximately 220 kDa with an isoelectric point of 4.8, which was composed of dimeric protein of 105 kDa. The enzyme was stable up to
and the presence of glycerol significantly increased its thermostability. The enzyme was found to hydrolyze both
in addition to
glucose and catalyzed glucosyl transfer to cellobiose. The enzyme attacked laminarin in an exotype-like fashion. The apparent Km's of the enzyme toward cellobiose, laminaribiose, laminarin were 0.53 mM, 0.35 mM and 1.11 mM, respectively. Glucose and glucono-
were competitive inhibitors for the enzyme. Copper (
), mercury (
) and p-chloromercuribenzoate were strong inhibitors of the enzyme. The immunoblotting result revealed that one form of
was biosynthesized, irrespective of carbon sources used. Polyacrylamide gel electrophoresis analysis of the in vitro translated product of total RNA from avicel grown mycelium established that the P. verruculosum
precursor was approximately 95 kDa in size. The amino acid composition and N-terminal amino acid sequence are given.
Effects of Stirring and Addition of Chemical Compounds on Glycerolysis of Triglyceride in Reversed Micelles
Chang, Pahn-Shick ;
Journal of Microbiology and Biotechnology, volume 1, issue 3, 1991, Pages 197~201
Glycerolysis of triolein by lipase from Chromobacterium viscosum lipase was studied batchwise in AOT-isooctane reversed micelles. The reaction mixture was extracted with chloroform and the content of triolein, 1, 2-diolein, 1, 3-diolein, 1-monoolein, and free fatty acid in the condensed chloroform solution was determined using high performance liquid chromatography (HPLC). The effect of agitation speed on the initial rate of conversion was examined. As the speed of agitation increased up to 700 rpm, the reaction rate increased. However, above 700 rpm, the rate approached maximum and did not increase that much. The glycerolysis activity and the stability of the enzyme were affected by stirring and addition of histidine or copper. Addition of histidine and copper increased the rates of glycerolysis but they are detrimental to the operational stability in reversed micelles.
Optimum Conditions for the Formation of Acetoin as a Precursor of Tetramethylpyrazine during the Citrate Fermentation by Lactococcus lactis subsp. lactis biovar. diacetilactis FC1
Kim, Kyoung-Heon ; Lee, Hyong-Joo ;
Journal of Microbiology and Biotechnology, volume 1, issue 3, 1991, Pages 202~206
To produce acetoin as a precursor of the tetramethylpyrazine flavor compound from citrate by Lactococcus lactis ssp. lactis biovar. diacetilactis FC1, fermentation factors such as inital pH of culture media, temperature, concentration of Na-citrate, thiamin-HC1 and sugars were examined. The best acetoin production was achieved with initial pH in the culture media of 5.5, fermentation temperature of
, Na-citrate concentration of 3%, addition of thiamin-HC1 at 2 mg/l and galactose as a carbon source. When fermentation was carried out under the optimum conditions, the exhaustion of Na-citrate and the production of acetoin took simultaneously and acetoin reached the maximum content, 80 mmole/l after 20 hours.
The Growth, Effect of COD-Reduction, and Flocculation Characteristics of Candida rugosa in Sugar Beet Stillages
Lee, Ki-Young ;
Journal of Microbiology and Biotechnology, volume 1, issue 3, 1991, Pages 207~211
Yeast fermentation at
was conducted for microbial protein production and COD reduction in three different sugar beet stiIlages by a thermo- and acid-tolerant yeast Candida rugosa isolated from East Africa. The assimilation proceedings of some main components such as protein, carbohydrate, total titrable acids and glycerol in stillages were observed with growth kinetics of the yeast. Most of glycerol and organic acids were rapidly assimilated at the beginning of the fermentation. Protein assimilation was slowly accelerated with the proceeding of fermentation time and its assimilation rate reached only 14.2%-28.4%. Though Candida rugosa was a flocculent yeast, the flocculation characteristics of the yeasts grown in three stillages were different from each other.
Yeast Single-Cell Protein Production Using Potato Processing Waste Water
Park, Eung-Yeal ; Crawford, Don-L. ; Korus, Roger-A. ; Heimsch, Richard-D. ;
Journal of Microbiology and Biotechnology, volume 1, issue 3, 1991, Pages 212~219
Four species of yeast, Saccharomyces cerevisiae, Candida utilis, Saccharomycopsis flbuligera, and Schwanniomyces castellii were evaluated for their ability to bioconvert potato processing waste water into microbial protein and the resulting single-cell proteins were evaluated as protein sources for rainbow trout, using in vitro analyses. The studies indicated that Schwanniomyces castellii, which utilizes starch dircetly and converts it into cell mass efficiently, was suitable for the bioconversion. In the single-stage continuous bioconversion, the yield S. castellii cell mass, which contained approximately 37% protein, was 77%, at dilution rate 0.25
. Reduction of total carbohydrate was 81%. During batch fermentations, cell mass yield was about 72% and total carbohydrate reduction was 81%. Among the yeasts tested, S. castellii possessed the most fragile cell wall and had a favorable amino acid profile for salmonid fish; protein score of 86% (Met). In an in vitro pepsin digestibility test 80% digestibility (23~38% above control) was observed when cells were pre-heated in a steam bath for 30 min. Results presented should be regarded as being preliminary in nature because they were derived from single experiments.
Elicitation and In Situ Recovery of Alkaloids in Suspension Cultures of California Poppy
Byun, Sang-Yo ; Pedersen, Henrik ;
Journal of Microbiology and Biotechnology, volume 1, issue 3, 1991, Pages 220~226
Large and rapid increases in benzophenanthridine alkaloid production occured in suspension cultures of Eschscholtzia californica cells treated with elicitors. Response to different biotic elicitors showed that elicitors prepared from yeast extract, Collectotrichum lindemuthianum and Verticillium dahliae induced alkaloid formation. Highest alkaloid accumulation was obtained with
of yeast extract elicitor per gram of fresh cell weight. In time course performance after elicitor addition, more than 40 hours were required to obtain saturated alkaloid accumulation. Compounded silicone fluid, an ideal accumulation phase for two-phase culture of E. californica, accumulated a large amount of alkaloids produced in a specific manner. Elicitation in two-phase culture clearly increased net alkaloid production as well as their concentrations in the accumulation phase.