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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Microbiology and Biotechnology
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Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 12, Issue 6 - Dec 2002
Volume 12, Issue 5 - Oct 2002
Volume 12, Issue 4 - Aug 2002
Volume 12, Issue 3 - Jun 2002
Volume 12, Issue 2 - Apr 2002
Volume 12, Issue 1 - Feb 2002
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Effect of Chitinase-Producing Paenibacillus illinoisensis KJA-424 on Egg Hatching of Root-Knot Nematode (Meloidogyne incognita)
Jung, Woo-Jin ; Jung, Soon-Ju ; An, Kyu-Nam ; Jin, Yu-Lan ; Park, Ro-Dong- ; Kim, Kil-Yong ; Shon, Bo-Kyoon ; Kim, Tae-Hwan ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 865~871
A bacterium having strong chitinolytic activity on
colloidal chitin-containing agar medium was isolated from coastal soil in Korea. Based on the nucleotide sequence of conserved segment of a 165 rRNA gene, the bacterium was identified as Paenibacillus illinoisensis KJA-424. The population of P. illinoisensis KJA-424 and chitinase activity significantly increased for the first 2 days of incubation. On SDS-PACE analysis with
glycol chitin, three protein bands (63, 54, and 38 kDa) with chitinolytic activity were detected tooted. The effect of P illinoisensis KJA-424 on the egg hatch of root-knot nematode (Meloidogyne incognita) was investigated. After 7 days of incubation with the chitinase-producing P. illinoisensis KJA-424, none of the eggs hatched, whereas a
egg hatching rate was observed in the water control. Inverted and scanning electron microscopic observations demonstrated that P. illinoisensis KJA-424 deformed and destroyed the eggshell of M. incognita. In conclusion, chitinase-produced by p. illinoisensis KJA-424 caused the lysis of M. incognita eggshell and resulted in the inhibition of egg hatching in vitro.
Hypolipidemic Effect of Exo- and Endo-Biopolymers Pmduced from Submerged Mycelial Culture of Ganoderma lucidum in Rats
Yang, Byung-Keun ; Jeong, Sang-Chul ; Song, Chi-Hyun ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 872~877
The hypolipidemic effect of the exe-biopolymer (EXBP) and endo-biopolymer (ENBP) produced from a submerged mycelial culture of Ganoderma lucidum was investigated in dietary-induced hyperlipidemic rats. Hypolipidemic effects were achieved in both the EXBP- and ENBP-treated groups, however, the former proved to be more potent than the latter. The administration of the EXBP (100 mg/kg body weight) substantially reduced the plasma total cholesterol, low-density lipoprotein (LDL) cholesterol, triglyceride, phospholipid levels, and atherogenic index by 31.0, 39.0, 35.4, 28.1, and 53.5%, respectively, when compared to the control group. The EXBP also lowered the liver total cholesterol, triglyceride, and phospholipid levels by 22.4, 23.1, and 12.9%, respectively. Furthermore, the high-density lipoprotein (HDL) cholesterol and ratio of HDL cholesterol to total cholesterol were significantly increased by as much as 24.2% and 47.6%, respectively.
Influence of Growth Rate on Biosorption of Heavy Metals by Nocardia amarae
Kim, Dong Wook ; Daniel K. Cha ; Hyung-Joon Seo ; Jong Bok Bak ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 878~881
The goal of the current research was to assess the influence of the growth rate of Nocardia amarae on its overall metal binding capacity. Batch sorption isotherms for cadmium (Cd), copper (Cu), and nickel (Ni) showed that Nocardia cells harvested from chemostat cultures at a dilution rate of
had a significantly higher metal sorption capacity than cells grown at 0.5 and
. The cell surface area estimated using a dye technique indicated that pure N. amarae cells grown at a lower growth rate had a significantly more specific surface area than cells harvested from a higher growth rate operation. Accordingly, this difference in the specific surface area seemed to indicate that the higher metal sorption capacity of the slowly growing Nocardia cells was due to their higher specific surface area.
Production of Hantaan Virus from Human Immortalized Retina Cell and Its Immunogenicity
Bae, Cheon-Soon ; Choi, Jun-Youl ; An, Chang-Nam ; Kim, Jong-Su ; Hur, Byung-Ki ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 882~889
Hantaan vims production, using human immortalized retina cell (PER. C6), was investigated to develop an inactivated virus vaccine. To infect Hantaan virus into PER. C6, two infection methods (medium-to-cell and cell-to-cell) were tried, and IFA results showed that the cell-to-cell infection method was very useful for producing Hantaan virus-infected PER, C6. Hantaan virus production was significantly affected by the growth rate of PER. C6 and the content of FBS in medium. Higher specific growth rate of infected PER. C6 and lower FBS content induced higher production of Hantaan virus. The inactivated human cell-culture vaccines with various EIA titers were prepared, their antibody responses were compared with those of inactivated suckling mouse brain vaccines (
). and the result showed their immunogenicities were slightly higher than those of inactivated suckling mouse vaccines. Therefore, this study shows the possibility of the development of Hantaan virus vaccine from a human cell culture.
Purification, Characterization, and cDNA Cloning of Xylanase from Fungus Trichoderma Strain SY
Min, Shin-Young ; Kim, Bong-Gyu ; Lee, Chan ; Hur, Hor-Gil ; Ahn, Joong-Hoon ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 890~894
A xylanase-producing Trichoderma strain was isolated from soil. Xylanase from Trichoderma strain SY was purified 21-fold to an apparent homogeneity, with a
yield. The optimum pH and temperature were determined to be 5.5 and
, respectively, and its molecular weight was 21-kDa by SDS-PAGE. The corresponding gene, named xyl, was cloned by RT-PCR. DNA blot analysis of xyl showed that this gene is present as a single copy. The amino acid sequence of the Xyl protein showed similarity to those of other xylanases derived from various fungi. mRNA of xyl was highly expressed when this fungus was grown on cellulose or xylan as a sole carbon source, but undetectable when grown on sucrose. Extracts of Escherichia coli cells expressing xyl were found to have xylanase activity. It was confirmed that xyl from this isolate encodes xylanase.
-Glucosidase Inhibitor by
-Glucosidase Inhibitor-Producing Bacillus lentimorbus B-6
Kim, Kyoung-Ja ; Yang, Yong-Joon ; Kim, Jongkee ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 895~900
A soil microorganism producing
-glucosidase inhibitors was identified as Bacillus lentimorbus, based on the fatty acid and morphological analyses, along with biochemical and physiological tests. The
-glucosidase inhibitor was highly produced by this strain in a culture medium containing
of sodium glutamate and
of glucose, pH 8.0 at
for 2 days. The
-glucosidase inhibitor from culture filtrate of his strain was identified as water soluble, organic solvent nonextractable, and heat stable. In addition to
-glucosidase inhibitor, this strain also produced
-glucosidase inhibitor in he same culture medium and this inhibitor showed an antifugal activity against Botrytis cinerea. While the production of
- glucosidase inhibitor was decreased by a glucose concentration higher than
, the production of
-glucosidase inhibitor was lot Influenced by a glucose concentration higher than
-glucosidase inhibitor from culture filtrate of this strain was separated from the
-glucosidase inhibitor through Sephadex G-100 column chromatography.
Increased Microbial Resistance to Toxic Wastewater by Sludge Granulation In Upflow Anaerobic Sludge Blanket Reactor
Bae, Jin-Woo ; Rhee, Sung-Keun ; Kim, In S. ; Hyun, Seung-Hoon ; Lee, Sung-Taik ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 901~908
The relationship between the layered structure of granules in UASB reactors and microbial resistance to toxicity was investigated using disintegrated granules. When no toxic materials were added to the media, the intact and disintegrated granules exhibited almost the same ability to decrease COD and to produce methane. However, when metal ions and organic toxic chemicals were added to a synthetic wastewater, he intact granules were found to be more resistant to toxicity than the disintegrated granules, as determined by the methane production. The difference in resistance between the intact and disintegrated granules was maximal, with toxicant concentrations ranging from 0.5 mM to 2 mM for trichloroethylene with toluene and 5 mM to 20 mM for metal ions (copper, nickel, zinc. chromium, and cadmium ions). The augmented COD removal rate by granulation compared to disintegrated granules was also measured in the treatment of synthetic and real wastewaters; synthetic wastewater,
; municipal wastewater,
; swine wastewater,
; food wastewater,
; dye works wastewater,
; and landfill leachate,
. Continuous reactor operation also demonstrated that the granules in the UASB reactor were helpful in treating toxic wastewater, such as landfill leachate.
Substrate Utilization Patterns During BTEX Biodegradation by an o-Xylene-Degrading Bacterium Ralstonia sp. PHS1
Lee, Sung-Kuk ; Lee, Sun-Bok ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 909~915
The biodegradation of BTEX components (benzene, toluene, ethylbenzene, o-xylene, m-xylene, and p-xylene) individually and in mixtures was investigated using the o-xylene-degrading thermo-tolerant bacterium Ralsronia sp. strain PHS1 , which utilizes benzene, toluene, ethylbenzene, or o-xylene as its sole carbon source. The results showed that as a single substrate for growth, benzene was superior to both toluene and ethylbenzene. While growth inhibition was severe at higher o-xylene concentrations, no inhibition was observed (up to 100 mg
) with ethylbenzene. In mixtures of BTEX compounds, the PHS1 culture was shown to degrade all six BTEX components and the degradation rates were in the order of benzene, toluene, o-xylene, ethylbenzene, and m- and p-xylene. m-Xylene and p-xylene were found to be co-metabolized by this microorganism in the presence of the growth-supporting BTEX compounds. In binary mixtures containing the growth substrates (benzene, toluene, ethylbenzene. and o-xylene), PHS1 degraded each BTEX compound faster when it was alone than when it was a component of a BTEX mixture, although the degree of inhibition varied according to the substrates in the mixtures. p-Xylene was shown to be the most potent inhibitor of BTEX biodegradation in binary mixtures. On the other hand, the degradation rates of the non-growth substrates (m-xylene and p-xylene) were significantly enhanced by the addition of growth substrates. The substrate utilization patterns between PHS1 and other microorganisms were also examined.
Purification of Recombinant Human Alpha-2a Interferon Without Using Monoclonal Antibodies
Kim, Dong Chung ; Jin Jung ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 916~920
This report describes a high-level expression of human alpha-2a interferon (
) in Escherichia coli and its pilot scale purification by using a monoclonal antibody-independent chromatographic procedure that is based on anion-exchange, cation-exchange, hydrophobic interaction, and gel filtration. The recombinant E. coli produced much more
in a soluble form, when cultivated at low temperatures than at high-temperature fermentation. However, if the bacterial growth was taken into consideration, fermentation at
seemed optimal for the interferon production. By using our new protocol, we recovered approximately 160 mg of
with a specific activity of
IU/mg from 201 of the broth. The gel permeation chromatographic and SDS-PAGE indicated that the interferon preparation was purified to homogeneity and was of the correctly folded fast-migrating monomer.
Cloning and Characterization of Cycloinulooligosaccharide Fructanotransferase (CFTase) from Bacillus polymyxa MGL21
Jeon, Sung-Jong ; You, Dong-Ju ; Kwon, Hyun-Ju ; Shigenori Kanaya ; Namio Kunihiro ; Kim, Kwang-Hyeon ; Kim, Young-Hee ; Kim, Byung-Woo ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 921~928
Microorganism producing extracellular CFTase was isolated from soil and designated as Bacillus polymyxa MGL21. The gene encoding the CFTase (cft) from B. polymyxa MGL21 was cloned and sequenced. The ORF of the cf gene was composed of 3,999 nucleotides, encoding a protein (1,333 amino acids) with a predicted molecular mass of 149,375 Da. Sequence analysis indicated that CFTase was divided into five distinct regions. CFTase contained three regions of repeat sequences at the N-terminus and C-terminus. The endo-inulinase region of homology (ERH) of CFTase was similar to that of Pseudomonas mucidolens endo-inulinase (
identity, 259 amino acids). Furthermore, CFTase possessed a highly conserved core region, which is considered to be functional for the hydrolysis reaction of inulin. The cft gene was expressed in a His-tagged form in Escherichia coli cells, and the His-tagged CFTase was purified to homogeneity. The optimal temperature and pH for CFTase activity were found to be
and 9.0, respectively. The enzyme activity was completely inhibited by 10 mM
. Thin-layer chromatography analyses indicated that CFTase catalyzed not only the cyclization reaction ut also disproportionation and hydrolysis reactions as well.
Microbial Community Analysis of 5-Stage Biological Nutrient Removal Process with Step Feed System
Park, Jong-Bok ; Lee, Han-Woong ; Lee, Soo-Youn ; Lee, Jung-Ok ; Bang, Iel-Soo ; Park, Eui-So ; Park, Doo-Hyun ; Park, Yong-Keun ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 929~935
The 5-stage biological nutrient removal (BNR) process with step feed system showed a very stable organic carbon and nutrient removal efficiency (
phosphorus) for an operation period of 2 years. In each stage at the pilot plant, microbial communities, which are important in removing nitrogen and phosphorus, were investigated using fluorescence in-situ hybridization (FISH) and 165 rDNA characterization. All tanks of 5-stage sludge had a similar composition of bacterial communities. The totat cell numbers of each reactor were found to be around
of total 4,6-diamidino-2-phenylindol (DAPI) cells were hybridized to the bacterial-specific probe EUB388. Members of
-proteobacteria were the most abundant proteobacterial group, accounting for up to
. The high G+C Gram-positive bacterial group and Cytophaga-Flexibacter cluster counts were also found to be relatively high. The beta subclass proteobacteria did not accumulate a large amount of polyphosphate. The proportion of phosphorus-accumulating organisms (PAOs) in the total population of the sludge was almost
in anoxic-1 tank. The high G+C Gram-positive bacteria and Cytophaga-Flexibacter cluster indicate a key role of denitrifying phosphorus-accumulating organisms (dPAOs). Both groups might be correlated with some other subclass of proteobacteria for enhancing nitrogen and phosphorus removal in this process.
Synergistic Effects of Bacteriocin-Producing Pediococcus acidilactici K10 and Organic Acids on Inhibiting Escherichia coli O157:H7 and Applications in Ground Beef
Moon, Gi-Seong ; Kim, Wang-June ; Kim, Myung-Hee ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 936~942
When used in combination with organic acids, Pediococcus acidilactici K10 or its bacteriocin was effective in inhibiting Escherichia coli O157:H7 in vitro and in situ. P. acidilactici K10, a strain of bacteriocin-producing lactic acid bacteria (LAB), was previously isolated from kimchi in our laboratory, and the molecular weight of its bacteriocin was estimated to be around 4,500 Da by SDS-PAGE. Initially, P. acidilactici K10 and its bacteriocin could not inhibit E. coli O157:H7, when used alone. However, when they were used together with organic acids such as acetic, lactic, and succinic acids, they greatly inhibited E. coli O157:H7 in vitro. Based on these in vitro results, a real sample test with ground beef was conducted at
with acetic acid (0.25%) or lactic acid (0.35%) alone, and then in combination with P. acidilactici K10 (
CFU/g of sample). Combined treatment of P. acidilactici K10 with lactic acid showed the most inhibitory effect: a 2.8-
-unit reduction of E. coli O157:H7 in ground beef during storage at
. This result suggests that the combination of bacteriocin-producing P. acidilactici K10 and organic acids has great potential as a food biopreservative by inhibiting the growth of E. coli O157:H7.
Identification of a Domain in Yeast Chitin Synthase 3 Interacting with Chitin Synthase 4 by Two-Hybrid Analysis
Park, Hyun-Sook ; Shin-Jung-Choi ; Nok-Hyun-Park ; Chi-Hwa-Kim ; Sung-Uk-Kim ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 943~949
It has been proposed that chitin synthase 3 (CHS3)-nediated chitin synthesis during the vegetative cell cycle is regulated by chitin synthase 4 (CHS4) of Saccharomyces cerevisiae. To investigate direct protein-protein interaction between the coding products of these two genes, a domain of Chs3p that is responsible for interaction with Chs4p was identified, using the yeast two-hybrid system. This domain of 54 amino acids, termed MIRC3-4 (Maximum Interacting Region of Chs3p with Chs4p), is well conserved among CHS3 homologs of various fungi. Some mutations in MIRC3-4 resulted in a decrease in the enzymatic activity and chitin contents. Chs3p carrying those mutations exhibited weak interactions with Chs4p, when assayed by the yeast two-hybrid system. Surprisingly, all the mutants were sensitive to Calcofluor regardless of changes in enzymatic activities or chitin contents. This report deals with a core region in MIRC3-4 that affects the interaction with Chs4p.
Apoptotic Activity of Insect Pathogenic Fungus Paecilomycesc japonica Toward Human Acute Leukemia Jurkat T Cells is Associated with Mitochondria-Dependent Caspase-3 Activation Regulated by Bcl-2
Park, Hye-Won ; Jen, Do-Youn ; Kim, Young-Ho ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 950~956
The antitumor activity of the insect pathogenic fungus Paecilomyces japonica has been attributed to apoptotic cell death. However, the mechanism underlying the induced apoptosis has not yet been elucidated. In this study, we for the first time show that mitochondria-dependent caspase-3 activation were associated with the apoptotic activity of P. japonica in human acute leukemia Jurkat T cells. When Jurkat T cells were treated with the ethyl acetate extract of P japonica at concentrations ranging from
, apoptotic cell death. accompanied by several biochemical events such as caspase-9 activation, caspase-3 activation, degradation of poly (ADP-ribose) polymerase (PARP), and apoptotic DNA fragmentation, was induced in a dose-dependent manner. In addition, the release of cytochrome c from mitochondria was detected. Under these conditions, the expression of Fas and Fas-ligand (FasL) remained unchanged. Ethyl acetate extract-induced mitochondrial cytochrome c release, caspase-3 activation, PARP cleavage, and apoptotic DNA fragmentation were suppressed by the ectopic expression of Bcl-2, which is known to block mitochondrial cytochrorme c release. Accordingly, these results demonstrate that P. japonica-induced apoptotic cell death is mediated by a cytochrome c-dependent caspase-3 activation pathway that can be interrupted by Bcl-2.
Hypolipidemic Effect of Exo-Polymer Produced in Submerged Mycelial Culture of Five Different Mushrooms
Yang, Byung-Keun ; Park, Jun-Bo ; Song, Chi-Hyun ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 957~961
The hypolipidemic effect of exe-polymer produced in submerged mycelial culture of Hericium erinaceus (HE), Auricularia auricula-judue (AA), Flammulina veluripes (FV), Phellinus pini (PP), and Grifola frondosa (GF) was investigated in dietary-induced hyperlipidemic rats. The animals were administered with exe-polymers at the level of 100 mg/kg body weight daily for four weeks. Hypolipidemic effect was achieved in all the experimental groups, however, HE exo-polymer proved to be the most potent one, which significantly reduced the plasma triglyceride (
), total cholesterol (
), low-density lipoprotein (LDL) cholesterol (
), phospholipid (
), and liver total cholesterol (
) level, when compared to the saline administered (control) group. The results of the present investigation strongly demonstrate the potential of HE exe-polymer in combating hyperlipidemia in the experimental animals.
Simple Monodimensional Model for Linear Growth Rate of Photosynthetic Microorganisms in Flat-Plate Photobioreactors
Kim, Nag-Jong ; Suh, In-Soo ; Hur, Byung-Ki ; Lee, Choul-Gyun ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 962~971
The current study proposes a simple monodimensional model to estimate the linear growth rate of photosynthetic microorganisms in flat-plate photobioreactors (FPPBRs) during batch cultivation. As a model microorganism, Chlorella kessleri was cultivated photoautotrophically in FPPBRs using light-emitting diodes (LEDs) as the light sources to provide unidirectional irradiation in the photobioreactors. Various conditions were simulated by adjusting both the intensity of the light and the height of the culture. The validity of the proposed model was examined by comparing the linear growth rates measured with the predicted ones obtained from the proposed model. Accordingly, the value of
was proposed as an approximate index for strategies to obtain the maximal lightn yield under light-limiting conditions for high-density algal cultures and as a control parameter to improve the photosynthetic productivity and efficiency.
Purification and Characterization of 2,4-Dichlorophenol Oxidizing Peroxidase from Streptomyces sp. AD001
Jeon, Jeong-Ho ; Yun-Jon Han ; Tae-Gu kang ; Eung-Soo Kim ; Soon-Kwang Hong ; Byeong-Chul Jeong ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 972~978
Streptomyces sp. AD001 is a Gram-positive soil actinomycetes secreting an uncharacterized 2,4-dichlorophenol (DCP) oxidizing enzyme, whose activity is similar to the previously known Actinomycetes lignin-peroxidase (ALiP). This extracellular peroxidase was purified from Streptomyces sp. AD001 as a single protein band on an SDS-PACE by ammonium sulfate fractionation, Q-sepharose, concanavalin A, and Bio-Gel HTP column chromatographies. The molecular mass of the purified peroxidase was determined by SDS-PAGE to be 45.2 kDa, and 49.7 kDa with MALDI-TOF-MS, respectively. The highest level of peroxidase activity was observed at pH 7.5 and
. The amino terminal sequence of the purified peroxidase (G-E-P-E-E-G-N-V-D-G-T-L) showed no significant homologies to my known proteins, suggesting that Streptomyces sp. AD001 may secrete a novel kind of bacterial peroxidase Initial rate kinetic data of the 2,4-DCP oxidation were best modeled with a random-binding bireactant system.
Nitrogen Removal from Wastewaters by Microalgae Without Consuming Organic Carbon Sources
Lee, Kwang-Yong ; Lee, Choul-Gyun ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 979~985
The possibility of microalgal nitrogen treatment was tested in wastewaters with a low carbon/nitrogen (C/N) ratio. Chlorella kessleri was cultured in the two different artificial wastewaters with nitrate as a nitrogen source: one contained glucose for an organic carbon source and the other without organic carbon sources. The growth rates of the two cultures were almost identical when the aeration rate was over 1 vvm. These results suggest that microalgae could successfully remove nitrogen from wastewater, as far as the mass transfer of
, was not limited. Nitrate was successfully reduced to below 2 mg
-N/ml from the initial nitrate concentration of 140 mg
-N/ml in 10 days, even in the wastewater with no organic carbon source. Similar results were obtained when ammonium was used as the sole nitrogen source instead of nitrate. Higher concentrations of nitrogen of 140, 280, 560 and 1,400 mg/ml were also tested and similar amounts of nitrogen were removed by algal cultures without showing any substrate inhibition.
Simple Purification of shiga Toxin B Chain from Recombinant Escherichia coli
Oh, Young-Phil ; Jeong, Seong-Tae ; Kim, Dae-Weon ; Kim, El-Chae ; Yoon, Ki-Hong ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 986~988
A plasmid expression vector of pEStxl encoding a mature form of the B chain of the Shiga toxin was constructed without a signal peptide under the control of an inducible n promoter. The encoded protein was purified to 90% by simple heat treatment, and then further purified to 95% by Phenyl-Sepharose and DEAE-Sepharose chromatographies, all in a single day. Accordingly, this expression system and heat treatment could facilitate the rapid purification of gram-scale amounts of the Shiga toxin B subunit from recombinant Escherichia coli cells.
Development of Rapid Molecular Detection Marker for Colletotrichum spp. in Leaf and Fruit Tissues of Sweet Persimmon
Iee, Sang-Pyo ; Lee, Youn-Su ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 989~992
Sweet persimmon (Diospyros kaki Thunb.) is widely cultivated in the southern part of Korea and its cultivation is increasing. However, anthracnose disease caused by Colletotricuhum species is one of the major hinderances to the cultivation and production of sweet persimmon. Therefore, in the current study, PCR was used to specifically detect Colletotrichum spp., based on the sequences of the ITS II regions in the rDNA. Using the sequence data, CO-1 was designated to detect Colletotrichum together the with ITS 4 primer. The result showed that a single segment of ca. 500 bp was observed only in Colletotrichum, but not in any other fungal and bacterial isolates. The annealing temperatures and template DNA quantites were also investigated to identify optimal conditions for detection. Using these species-specific primers, a unique band was obtained at annealing temperatures ranging from
and template DNA levels from 10 pg-
Glucanhydrolase from Lipomyces starkeyi KSM 22 as Potential Mouthwash Ingredient
Kim, Doman ; Ryu, Su-Jin ; Son, Eun-Ju ; Chung, Hyun-Ju ; Kim, Seung-Heuk ; Kim, Do-Won ; Day, Donal-F. ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 993~997
A glucanhydrolase (a DXAMase exhibiting both dextranolytic and amylolytic activities) from Lipomyces starkeyi KSM 22 hydrolyzed polysaccharides having
-D-glucosidic linkages. The oral hygiene benefits of DXAMase-containing mouthwash were examined in relation to human experimental gingivitis during a 3-week period without brushing. The DXAMase-treated group exhibited a lower increase in plaque accumulation and gingival index score than the chlorhexidine-treated group. The DXAMase-treated group also showed less tongue accumulation, bad taste, and tooth staining, thus indicating a positive role for DXAMase as an antiplaque agent ingredient.
Antitumor Activity of Lactobacillus plantarum Cytoplasm on Teratocarcinoma-Bearing Mice
Kim, Ji-Yeon ; Woo, Hee-Jong ; Kim, Kyoung-Heon ; Kim, Eung-Ryool ; Jung, Hoo-Kil ; Juhn, Ho-Nam ; Lee, Hyong-Joo ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 998~1001
Potential antitumor activity of Lactobacillus plantarum cytoplasm was examined using F9 teratocarcinoma-bearing BALB/C mice. The cytoplasmic fraction of L. plantarum was separated by sonication followed by ultracentrifugation. The fraction at a dose of 100 or 200 mg/kg/day was orally administered for 7 consecutive days before or after tumor inoculation to 16 mice. As a control, heat-killed whole cell was used at a dose of 100 mg/kg/day. Upon oral administrations of both the cytoplasm and heat-killed whole cell, when performed after and before tumor inoculation, the survival of F9-bearing mice prolonged more effectively. Administration of the cytoplasm after tumor inoculation extended the average survival days by 30 and
at daily dosages of 100 and 200 mg/kg/day, respectively. This result suggests that the cytoplasmic fraction of L. plantarum has strong antitumor activity against mouse F9 teratocarcinoma in vivo.
Overproduction of Bacillus macerans Cyclodextrin Glucanotransferase in E. coli by Coexpression of GroEL/ES Chaperone
Kwon, Mi-Jung ; So-Lim Park ; Sung-Koo Kim ; Soo-Wan Nam ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 1002~1005
The effects of GroEL/ES chaperone on the production of soluble form of B. macerans cyclodextrin glucanotransferase (CGTase) in recombinant E. coli were investigated. The cgt gene and groEL/ES genes are under the control of T7 promoter and Pzt-1 promoter, respectively. The optimal concentrations of inducers, IPTG and tetracycline, were found to be 1.0 mM and 10 ng/ml, respectively. When tetracycline and IPTG were added at the early exponential phase (2h) and exponential phase (3h) of growth, respectively, about 1.5-fold increase of soluble CGTase activity and 1.6-fold increase of soluble CGTase protein were obtained. An SDS-PAGE analysis revealed that about
of total CGTase protein was in the soluble fraction when GroEL/ES chaperone was overexpressed.
Evaluation of Antimicrobial Activity of Farnesoic Acid Derivatives
Kim, Sang-Hee ; Oh, Ki-Bong ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 1006~1009
The biological activities of farnesoic acid derivatives against pathogenic fungi and bacteria were investigated. Farnesoic acid and its derivatives showed growth inhibitory activities against various bacteria. Among the compounds tested, geranylgeranoic acid (3) had potent antibacterial activity against Salmonella typhimurium, Proteus vulgaris, and Bacillus subtilis with minimum inhibitory concentration (MIC) in the range of
. On the other hand, amide derivatives of farnesoic acid showed some antifungal activities. In particular, 3,7,11-trimethyl-dodeca-2,6,10-trienoic acid amide (5a) had a potent antifungal activity against Aspergillus niger, Candida albicans, and Trichophyton sp. with MIC in the range of
Low-Dose Gamma Irradiation as Means of Isolating Carotenoid-Hyperproducing Yeast Mutant
Sun, Nam-Kyu ; Lee, Seung-Hee ; Ahn, Gil-Hwan ; Won, Mi-Sun ; Song, Kyung-Bin ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 1010~1012
In order to isolate carotenoid-hyperproducing yeast, low-dose gamma irradiation was used as means of mutagenesis. Phaffia rhodozyma was treated by gamma irradiation of less than 10 kGy, which is considered to be a wholesome irradiation condition established by the Food and Drug Administration. Through repeated rounds of gamma irradiation and visual screening, mutant 3A4-8 was obtained. It produced a
carotenoid/g yeast, 69% higher content than
yeast of the unirradiated one. This result indicates that low-dose gamma irradiation could be used as means of mutagenesis to obtain carotenoid-hyperproducing strain of Phaffia rhodozyma, since only carotenoid-hyperproducing yeast survived gamma irradiation by scavenging oxygen radicals generated by radiolysis of water.
Inhibition of DNA Topoisomerase I by Cyclo(L-Prolyl-L-Phenylalanyl) Isolated from Streptomyces sp. AMLK-335
Rhee, Ki-Hyeong ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 1013~1016
Cyclo(L-prolyl-L-phenylalanyl) [cyclo(pro-phe)] was isolated from Streptomyces sp. AMLK-335 and found to inhibit DNA topoisomerase I activity. In a DNA relaxation assay using supercoiled pBR322 DNA, cyclo(pro-phe) inhibited the DNA topoisomerase activity more strongly than camptothecin, a known topoisomerase inhibitor. However, at a concentration of
, cyclo(pro-phe) produced a lower degree of DNA relaxation than camptothecin, therefore, the inhibition of topoisomerase I activity by cyclo(pro-phe) was also found to be dose dependent. Accordingly, the current results suggest that cyclo(pro-phe) may be a novel inhibitor of topoisomerase I.
In Vitro Bactericidal and Anticancer Activity of New Metabolite, ARK42, Isolated from Aspergillus repens K42
Park, Je-Won ; Song, Beom-Seok ; Ryu, Do-Jin ; Lee, Chan ; Kim, Young-Bae ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 1017~1021
A novel antibacterial metabolite, ARK42, was elated from a xerophilic fungal strain K42, and Identified as Aspergillus repens based on its morphological characteristics. The metabolite exhibited antibacterial activities towards Staphylococcus aureus, Bacillus cereus, and Pseudomonas aeruginosa, with MICs of 25, 12.5, and
, respectively, and killed Pseudomonas aeruginosa with minimal bactericidal concentration (MBC) of
. Furthermore, anticancer activities were demonstrated against human colon cancer DLD- 1 and lung cancer LXFL529 cells with an
of 10 and
Antifungal Activities Against Plasmodiophora brassicae Causing Club Root
Kim, Bum-Joon ; Choi, Gyung-Ja ; Cho, Kwang-Yun ; Yang, Hee-Jung ; Shin, Choon-Shik ; Lee, Chul-Hoon ; Lim, Yoong-Ho ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 1022~1025
Club root is one of the major diseases that occur in crucifers. It is caused by Plasmodiophora brassicae. In order to discover microbial biopesticides against P. brassicae, forty-eight Streptomyces isolated from soil were screened. Among these, three strains showed excellent pesticidal activities. We report results on in vivo screening with fermentation broths of these strains and identification of the strain taxa.
Streptomyces with Antifungal Activity Against Rice Blast Causing Fungus, Magnaporthe grisea
Lee, Chul-Hoon ; Kim, Bum-Joon ; Choi, Gyung-Ja ; Cho, Kwang-Yun ; Yang, Hee-jung ; Shin, Choon-Shik ; Min, Shin-Young ; Lim, Yoon-Gho ;
Journal of Microbiology and Biotechnology, volume 12, issue 6, 2002, Pages 1026~1028
Screening tests against fungus causing rice blast, Magnaporthe grisea, were performed in order to develop biopesticides. More than 400 actinomycetes collected at several sites near Hanla Mountain on Jeju Island, Korea were tested, and strain BG2-53 showed potent antifungal activity. The in vivo screening was performed with fermentation broth, and the strain taxon was identified.