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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Microbiology and Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 5, Issue 6 - Dec 1995
Volume 5, Issue 5 - Oct 1995
Volume 5, Issue 4 - Aug 1995
Volume 5, Issue 3 - Jun 1995
Volume 5, Issue 2 - Apr 1995
Volume 5, Issue 1 - Mar 1995
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Nucleotide Sequence Analysis of an Endo-Xylanase Gene (xynA) from Bacillus stearothermophilus
Choi, Yong-Jin ; Cho, Ssang-Goo ;
Journal of Microbiology and Biotechnology, volume 5, issue 3, 1995, Pages 117~124
A gene (xynA) encoding the endo-xylanase (E.C.22.214.171.124) from Bacillus stearothermophilus was cloned in E. coli, and its complete nucleotide sequence was determined. The xynA gene consists of a 636 base pairs open reading frame coding for a protein of 212 amino acids with a deduced molecular weight of 23, 283 Da. A putative signal sequence of 27 amino acid residues shows the features comparable with the Bacillus signal sequences; namely, the signal contains a positively charged region close to the N-terminus followed by a long hydrophobic string. The coding sequence is preceded by a possible ribosome binding site with a free energy value of -16.6 kcal/mol and the transcription initiation signals are located further upstream. The translation termination codon (TAA) at the 3 end of the coding sequence is followed by two palindrome sequences, one of which is thought to act as a terminator. The xynA gene has a high GC content, especially in the wobble position of codons (64%). Comparison of the primary protein sequence with those of other xylanases shows a high homology to the xylanases belonging to family G.
Nucleotide Sequence on Upstream of the cdd Locus in Bacillus subtilis
JONG-GUK KIM ; KIM, KYE-WON ; SEON-KAP HWANG ; JOO-WON SUH ; BANG-HO SONG ; SOON-DUCK HONG ;
Journal of Microbiology and Biotechnology, volume 5, issue 3, 1995, Pages 125~131
A 3, 346 bp of the cdd upstream region in Bacillus subtilis was sequenced from the pSO1 (Song BH and J Neuhard. 1989. Mol. Gen. Genet 216: 462-468) and sequence homology was searched to the known genes in Genbank and European Molecular Biology Laboratory databanks. Five complete and one truncated putative coding sequences deduced from the nucleotide sequence were found through the ORF searching by Genetyx and Macvector software, and one of them was identified as the dgk (diacylglycerol kinase) gene and another, a truncated one, as the phoH (phosphate starvation-inducible gene) gene. The B. subtilis dgk gene, having a role for response to several environmental stress signals, revealed an open reading frame of 134 amino acids with 43.1% of sequence identity to the Streptococcus mutans dgk gene. The carboxy terminal 59 residues of the truncated phoH gene showed 52.7% and 34.5% of sequence identity in amino acids with the corresponding genes of Mycobacterium leprae and Escherichia coli. The four remaining coding sequences consisting of 115, 421, 91, and 91 residues were thought to be unknown ORFs because they have no significant similarity to known genes.
Preincubation without attB DNA inhibits In Vitro Integrative Recombination of P 1 Mutant attP DNA of Bacteriophage Lambda
Yoo, Seung-Ku ;
Journal of Microbiology and Biotechnology, volume 5, issue 3, 1995, Pages 132~137
The lambda integrase (lnt) is believed to bind to several arm and core sites of attP DNA in order to facilitate intasome formation. We have done systematic mutagenic analysis on all 5 arm sites and found that P1 is absolutely required for integration while P2 is not. We also found that all 3 P' arm sites(P'1, P'2, and P'3) are required for efficient integrative recombination. P'1, which is an important binding site for excision, also seems to be crucial for integration when preincubation of attP DNA with Int and IHF is performed before recombination. Preincubation assay revealed that preincubation with Int and IHF improved the efficiency of recombination of wild type attP DNA and demolished recombinations of P'1 mutant attP DNAs.
Isolation and Characterization of Bacillus thuringiensis Strain BT-209 producing Cuboidal
Jung, Yong-Chul ; Kim, Sung-uk ; Son, Kwang-Hee ; Lee, Hyung-Hoan ; Bok, Song-Hae ;
Journal of Microbiology and Biotechnology, volume 5, issue 3, 1995, Pages 138~142
Bacillus thuringiensis strain BT-209 was isolated from a soybean grain dust sample in Korea. The strain BT-209 produced two different sizes of cuboidal crystals and one spore in the cell. In the biochemical characterization, the strain BT-209 showed negative reactions on the production of urease, and the utilization of citrate and sucrose. Examination of its antibiotic resistance revealed that while the strain BT-209 showed higher sensitivity than B. thuringiensis subsp. kurstaki HD-1 to ampicillin, bacitracin, chlortetracycline, gentamycin, neomycin, penicillin G, tetracycline and tobramycin, it was more resistant to methicillin than B. thuringiensis subsp. kurstaki HD-1. The
-endotoxin crystal of strain BT-209 consisted of three proteins with apparent molecular weights of appoximately 148, 135 and 62 kDa on a 10% SDS-PAGE. The strain BT-209 had at least eight different plasmids with sizes of 4.1, 5.2, 6.3, 8.6, 14.6, 24.5, 67.6 and 77.6 Kb. The strain BT-209 showed strong lethalities of 70% and 87% against Bombyx mori and Hyphantria cunea larvae. at 72 h, respectively.
Volatile Components of Korean Soybean Paste Produced by Bacillus subtilis PM3
JONG-KYU KIM ; JI, WON-DAE ; SUNG-HO YANG ; MYEONG-RAK CHOI ;
Journal of Microbiology and Biotechnology, volume 5, issue 3, 1995, Pages 143~148
A strain producing soybean paste flavor was isolated from traditional Korean soybean paste. The isolate was identified as Bacillus subtilis PM3. The neutral fraction representing the traditional soybean paste aroma was obtained from the whole volatile components produced by B. subtilis PM3 in cooked soybean. Each separated peak from the neutral fraction of gas chromatogram was identified by gas chromatography-mass spectrometry (GC/MS) and Kovat's retention index, and the aromas of each peak were investigated by a sniffing test with the exercise panel. The twenty-nine components, including six character impact compounds and twelve components of flavors of Korean soybean paste, were confirmed. Some regions of gas chromatogram represented the soybean paste odor. It has been confirmed that traditional Korean soybean paste can be manufactured with the strain B. subtilis PM3.
Culture Conditions and Growth Characteristics of Bifidobacterium longum
KIM, WANG-JUNE ; SEONG-KWAN CHA ;
Journal of Microbiology and Biotechnology, volume 5, issue 3, 1995, Pages 149~153
A simple and low-cost medium was developed for the growth of Bifidobacterium longum KFRI 977. Of three bifidobacterial strains, B. longum KFRI 977 (ATCC 15707) showed the best growth in MRSC broth containing 0.3% oxgall, grew well in partially anaerobic condition, exhibited highest
-galactosidase activity, and was inhibitory against Clostridium perfringens KFRI 434. Of three developed media, the population of B. longum KFRI 977 was highest (1.9
/ml) in ISP based medium. The composition of ISP based medium is ISP (5%), glucose (1%), L-cysteine HCI (0.05%), Trypticase peptone (0.5%), yeast extract (0.5%),
(0.05%), Tween-80 (0.1%), and phosphate buffer (pH 7.0). Hydrolysis of ISP by Protease A was unnecessary, and the use of phosphate buffer (pH 7.0) prevented the formation of protein precipitate. Associative culture of B. longum KFRI 977 with Lactobacillus acidophilus KFRI 233 was proven to be deleterous to the growth of B. longum KFRI 977.
-Amastatin, MRK-22 as an Inhibitor of Aminopeptidase M produced by Streptomyces sp. SL20209
Kho, Yung-Hee ; Ko, Hack-Ryong ; Chun, Hyo-Kon ; Kim, Seung-Ho ; Sung, Nack-Kie ;
Journal of Microbiology and Biotechnology, volume 5, issue 3, 1995, Pages 154~157
MRK-22, an inhibitor of aminopeptidase M was isolated from the culture broth of Streptomyces sp. SL20209. The structure of MRK-22 was defined to be 3-amino-2-hydroxy-5-methylhexanoyl-valyl-valine, des-
-amastatin, by spectroscopic analysis and this was also confirmed by solid phase synthesis of the inhibitor. The molecular formula and weight of MRK-22 were
and MW 359(
), respectively, and its
value against hog kidney AP-M was 0.79
Fermentation of MR-387A and H, Novel Aminopeptidase M Inhibitors by Streptomyces sp. SL-387 : Carbon and Nitrogen Catabolite Repression of Inhibitor Formation
Kho, Yung-Hee ; Chung, Myung-Chul ; Chun, Hyo-Kon ; Lee, Choong-Hwan ; Lee, Ho-Jae ; Kim, Su-Il ;
Journal of Microbiology and Biotechnology, volume 5, issue 3, 1995, Pages 158~162
The effect of carbon and nitrogen sources on the production of novel aminopeptidase M inhibitors MR-387A and B by Streptomyces sp. SL-387 has been studied. High D-glucose and ammonia concentrations (5$\%$ and 1$\%$, respectively) exerted a negative influence on the inhibitor formation. The suppressive effect of glucose on the inhibitor formation is probably caused by an effect of medium pH rather than that of cyclic AMP. To establish the optimum conditions for inhibitor overproduction, various nitrogen sources and ammonium ion-trapping agents were examined. The use of ammonia slow-releasing nitrogen sources such as soybean meal and fish meal, or ammonium ion-trapping agents such as kaoline, celite, and natural zeolite achieved the enhancement of inhibitor production. These results also indicate that inhibitor formation is affected by ammonium ion repression.
Chemical Modification of Macroporous Gelatin Microcarriers and Characterization of Cell Growth and Attachment
Lim, Hyun-Soo ; Kim, Jung-Hoe ;
Journal of Microbiology and Biotechnology, volume 5, issue 3, 1995, Pages 163~166
Chemical modification of gelatin-based macroporous microcanier beads was achieved by increasing the charge density through incorporation of (diethylamino)ethylchloride-hydrochloride (DEAE:CI-HCI) or lysine, and this significantly improved the attachment and growth of HepG2 cells. When microcarriers were modified by the addition of 2% lysine, positive charge density was 0.95 meq/g-caniers. In case of modification of microcarriers with DEAE:CI-HCI, positive charge density was 0.6 meq/g-caniers. An increase in charge density of the microcaniers to improve cell attachment has facilitated the growth of the cells on macroporous gelatin microcaniers. Also, final HepG2 cell concentration cultivated on modified beads with DEAE:CI-HCI was increased up to
cells/ml. This was 2-3 times higher than that obtained with unmodified macroporous gelatin microcarriers.
Isolation and Characterization of a Methylotroph Producing 3-hydroxybutyrate-3-hydroxyvalerate Copolymer
JUNG HOE KIM ; KIM, PIL ; SEON WON KIM ; GYUN MIN LEE ; HYUN SOO LEE ;
Journal of Microbiology and Biotechnology, volume 5, issue 3, 1995, Pages 167~171
A bacterial strain C-02 using methanol as a carbon source was isolated from Gumi Industrial Estate and selected based on its rapid growth and capability of poly-
-hydroxybutyrate accumulation. Characteristics of strain C-02 showed that it belongs to the Methylococcaceae family, Type II subgroup. Strain C-02 could incorporate valerate into the PHB chain to form 3-hydroxybutyrate and 3-hydroxyvalerate (P(3HB-co-3HV)). Among various nutrient limitation tests, the nitrogen limitation test resulted in the highest content of P(3HB-co-3HV) per dry cell weight, 50
. Under the nitrogen limited condition, the average molecular weight of P(3HB-co-3HV) obtained was determined to be approximately
Effect of Aspergillus niger Pellets on Citric Acid Production in a Bubble Column Bioreactor
Kim, Seung-Hwan ; Yoo, Young-Je ; Kim, Eui-Yong ; Kim, Min-Hong ;
Journal of Microbiology and Biotechnology, volume 5, issue 3, 1995, Pages 172~176
Citrate is mainly produced from fungi and oxygen transfer has been known as one of the important factors in citric acid production. A bubble column bioreactor was used for citrate production after pellet was initially made using a stirred bioreactor for the inoculation. The relationship between the pellet size of Aspergillus niger and the oxygen transfer was elucidated by considering morphological characteristics of the pellet. The pellet size was determined by adjusting the impeller speed in the stirred bioreactor and the optimum diameter of the pellet was observed to be 2.2 mm under the experimental conditions. Pellet was maintained quite stable in the bubble column bioreactor and production of citric acid was significantly improved by maintaining optimal pellet conditions in the bubble column bioreactor.
Effects of Hydrogen Peroxide Concentration on the Polymerization of p-Phenylphenol in Organic Solvent by Peroxidase
Yoo, Young-Je ; Yeo, Joo-Sang ; Park, Tae-In ;
Journal of Microbiology and Biotechnology, volume 5, issue 3, 1995, Pages 177~180
In horseradish peroxidase-catalyzing polymerization of phenol under the water/dioxane solvent system, the optimal concentration of hydrogen peroxide was found to be 10 mmol/I. Feeding of hydrogen peroxide at its optimal concentration improved the polymerization performance by reducing reaction time and increasing molecular weights. Monomer conversions and the molecular weights of the enzymatically produced polymer were in the ranges of 83.1~94.2
and 58000~68000, respectively.