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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Microbiology and Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 5, Issue 6 - Dec 1995
Volume 5, Issue 5 - Oct 1995
Volume 5, Issue 4 - Aug 1995
Volume 5, Issue 3 - Jun 1995
Volume 5, Issue 2 - Apr 1995
Volume 5, Issue 1 - Mar 1995
Selecting the target year
Bacterial Contamination and Its Effects on Ethanol Fermentation
Chang, In-Seop ; Kim, Byung-Hong ; Shin, Pyong-Kyun ; Lee, Wan-Kyu ;
Journal of Microbiology and Biotechnology, volume 5, issue 6, 1995, Pages 309~314
Samples were collected from a commercial ethanol production plant to enumerate the bacterial contamination in each step of a starch based ethanol production process. Though the slurry of raw material used in the process carried bacteria with various colony morphology in the order of
per ml, only the colonies of white and circular form survived and propagated through the processes to the order of
per ml at the end of fermentation. Almost all of the bacterial isolates from the fermentation broth were lactic acid bacteria. Heterofermentative Lactobacillus fermentum and L. salivarius, and a facultatively heterofermentative L. casei were major bacteria of an ethanol fermentation. In a batch fermentation L. fermentum was more detrimental than L. casei to ethanol fermentation. In a cell-recycled fermentation, ethanol productivity of 5.72 g
was obtained when the culture was contaminated by L. fermentum, whilst that of the pure culture was 9.00 g
. Similar effects were observed in a cell-recycled ethanol fermentation inoculated by fermentation broth collected from an industrial plant, which showed a bacterial contamination at the level of 10
cells per ml.
Transport of Sulfanilic Acid via Microbial Dipeptide Transport System
Hwang, Se-Young ; Ki, Mi-Ran ; Cho, Suk-Young ; Yoo, Ick-Dong ;
Journal of Microbiology and Biotechnology, volume 5, issue 6, 1995, Pages 315~318
Sulfanilic acid (4-aminobenzenesulfonic acid) alone is normally not permeant in bacteria but can be readily delivered via the microbial dipeptide transport system. A dipeptidyl derivative of this compound, L-phenylalanyl-L-2-sulfanilylglycine (PSG), prepared by attachment of its primary amino group to the phenylalanyl
-glycine moiety, appeared to have a Km of 0.125 mM and a Vmax of 1.9 nmoles/ml/min (
, 1.0) in Escherichia coli. From competitive spectrophotometric analysis, it was found that the type of amino acids in both of the N- and C-terminals affected the kinetic power of dipeptides. The growth inhibitory effect of PSG was over 7 times more potent than that of the sulfanilic acid against E. coli, suggesting that this potential inhibition was presumably due to the increased hydrophobic nature of the sulfanilyl dipeptide.
Phenylalanyl-2-Sulfanilylglycine as Substrate for Leucine Aminopeptidase Assay
Hwang, Se-Young ; Cho, Suk-Young ; Yoo, Ick-Dong ;
Journal of Microbiology and Biotechnology, volume 5, issue 6, 1995, Pages 319~323
A chromogenic mimic of phenlyalanyl-dipeptide, L-phenylalanyl-L-2-sulfanilylglycine (PSG), was synthesized and examined for its usability in leucine aminopeptidase (LAP) assay. The enzyme activity was easily determined by measuring the amount of diazotized adduct of sulfanilic acid released upon hydrolysis of PSG (
=18,000/M/cm). Under the experimental conditions employed, PSG showed a Km of 0.063 mM and a Kcat of 1683/min, assessable less than 0.1
g of LAP per milliliter. And the presence of aminopeptidase M (APM) was suggested to be negligible in LAP assay. This novel assay can circumvent the occasional yellow background in biological systems, i.e., serums, etc..
Numerical Identification of Streptomyces fIaveus Producing Antibiotic Substances Inhibitory to Plant Pathogenic Fungi
Lee, Jung-Yeop ; Kim, Beom-Seok ; Hwang, Byung-Kook ;
Journal of Microbiology and Biotechnology, volume 5, issue 6, 1995, Pages 324~334
The actinomycete strain A 11 was antagonistic to plant pathogenic fungi Phytophthora capsid and Magnaporthe grisea. Based on the diaminopimelic acid (DAP) type and morphological characteristics examined by scanning electron microscopy, the strain A 11 was confirmed to belong to the genus Streptomyces. Based on Willcox probability and similarity level, the strain A 11 was numerically identified as Streptomyces flaveus using TAXON program of Ward and Goodfellow. Antibiotic production of S. flaveus strain A 11 was most favorable when cultured on glycerol yeast extract peptone (GYP) agar for 20 days at
. The crude antibiotics from solid GYP agar cultures of the strain A 11 were most effective against Phytophthora capsici and Sclerotinia sclerotiorum among the fungi tested. Antifungal activity of the antibiotics against Alternaria solani, Botryosphaeria dothidea, Cercospora capsici, Magnaporthe grisea, and Rhizoctonia solani was somewhat high, whereas Colletotrichum gloeosporioides and Fusarium oxysporum f. sp. cucumerinum were rarely inhibited even at high concentrations.
Preventive Effect of Ebelactone B, an Esterase Inhibitor on Rice Sheath Blight Caused by Rhizoctonia solani
Chun, Hyo-Kon ; Ko, Hack-Ryong ; Moon, Hang-Sick ; Kho, Yung-Hee ;
Journal of Microbiology and Biotechnology, volume 5, issue 6, 1995, Pages 335~340
Two types of Rhizoctonia solani esterases induced by cutin hydrolysate were partially purified by ammonium sulfate precipitation and gel filtration. The esterase I with hydrolyzing activity toward both
palmitate and the esterase II with hydrolyzing activity toward only
butyrate were inhibited by ebelactone B, an esterase inhibitor produced by actinomycetes with
values of 0.01 and
, respectively. Spraying on rice seedling with ebelactone B at a concentration of
completely suppressed infection by R. solani. Ebelactone B could not protect the wounded rice seedling and did not show any inhibitory effect on the mycelial growth at a concentration of 1 mg/ml. These results indicate that ebelactone B, an esterase inhibitor protects rice plants from infection with R. solani by inhibition of penetration, not through fungitoxic or fungicidal effect.
Biological Control of Powdery Mildew by Antibiotic-producing Microorganisms Antagonistic to Erysiphe graminis
Lee, Yong-Se ; Wolf, G. ;
Journal of Microbiology and Biotechnology, volume 5, issue 6, 1995, Pages 341~345
Seventy four microorganisms, which have antagonistic activity against to Fusarium culmorum, were tested for their inhibitory effect on colony development of obligate biotroph Erysiphe graminis f. sp. hordei Marchal, the causal agent of powdery mildew on barley plants. Of these, 13 actinomycetes isolates were shown to reduce the colony development of mildew completely by application of their 10% cell-free culture filtrates on barley leaves. An Isolate, A252, was the most powerful antagonist and its antifungal activity was further assessed. The colony development of mildew was significantly reduced by application of the 1% cell-free culture filtrate of isolate A252. In comparison to the control, the protective and curative application of 10% cell-free culture filtrate from A252 showed 88.5% and 96.1% reduction of colony numbers respectively. By the protective application, 68.3% of the inhibition was observed after 9 days of treatment, thus showed prolonged inhibitory effect. In vitro test, complete inhibition of the mycelial growth of Microdochium nivale was achieved by the treatment of 1% A252 culture filtrate and 80.2% of inhibition was observed by the 0.1% treatment.
Isolation of Antibiotic-producing Bacteria Antagonistic to Fusarium oxysporum from Sesame-growing Soils and Evaluation of Their Antifungal Activity
Lee, Yong Se ; Ho Young Lee ; Chang Ho Lee ; Hee Sung Park ;
Journal of Microbiology and Biotechnology, volume 5, issue 6, 1995, Pages 346~352
For isolation of antibiotic-producing bacteria antagonistic to Fusarium oxysporum, a total of 327 microorganisms were screened from sesame-growing soils collected at various locations in Korea by the modified Herr's triple-agar-Iayer technique. Among the 36 bacterial isolates further screened by the dual culture test on tryptic soy agar, 10 were tested to show their antagonistic activity against 14 plant pathogenic fungi. Bacterial culture filtrates were shown either to inhibit some phytopathogenic fungal growth or to suppress F. oxysporum infection of sesame plants maintained in the green house. An isolate, B23, with the most prominent antagonistic activity was identified as Bacillus subtilis.
Production and Characterization of Monoclonal Antibodies to Escherichia coli (ATCC 8739)
Yoo, Dohng-Hyun ; Lee, Young-Ha ; Jung, Jae-Deuk ;
Journal of Microbiology and Biotechnology, volume 5, issue 6, 1995, Pages 353~358
Escherichia coli causes intestinal and extraintestinal infections and has been an indicator of fecal pollution in water and food. BALB/c mouse was immunized by injection of somatic E. coli (ATCC 8739) cells to produce monoclonal antibodies. Splenocytes of mouse were fused with myeloma cells (Sp2/0-Ag14). Two hybridomas secreting monoclonal antibodies were established after being cloned. In SDS-PAGE analysis of E. coli antigens 37 protein profiles appeared from 14 kDa to 182 kDa. Western blot analysis using polyclonal antibodies demonstrated that protein antigens of 41 kDa, 38.2 kDa and 31.7 kDa were immunodominant. Monoclonal antibodies DY-CM1 and DY-CM2 recognized 31.7 kDa and 2.0 kDa antigens in Western blot analysis, respectely.
Immunological Analysis of Antigenic Variation of Bacillus thuringiensis subsp. sotto during Sporulation and Crystallization
Cho, Jae Min ; Gi Bum Nam ; Soon Bok Hong ; Myung Hwan Cho ;
Journal of Microbiology and Biotechnology, volume 5, issue 6, 1995, Pages 359~363
The antigenic variation of B. thuringiensis subsp. satto have been investigated for 120 hours during sporulation and crystallization by using SDS-PAGE and Western blot. Most antigens of a vegetative cell were found to disappear as it was in sporulation and crystallization, but protein antigens of 46, 29, 27, and 21 kDa continued to be expressed. The new protein bands of 293, 138, 119, 75, and 68 kDa appeared on days 2 through 5 in modified GYS medium. They were thought to be involved in sporulation and crystallization. The protein of 138 kDa was found to be a major protein of both crystal and spore. The expression patterns were immunologically analyzed by Western blot. The polyclonal antisera against the intact crystal showed strong immunoreactivity to proteins with molecular masses of 293, 138, 68, and 46 kDa. The polyclonal antisera against the spore recognized proteins of 293, 138, 68, and 46 kDa. Both crystals and spores appeared to express the common protein antigens.
Enhanced Essential Oil Formation by Two-phase Culture of Mentha piperita Cells in Shake Flask and Air-lift Bioreactors
Kim, Teresa ; Kim, Tae-Yong ; Bae, Geun-Won ; Chae, Young-Am ; Lee, Hyong-Joo ; Chung, In-Sik ;
Journal of Microbiology and Biotechnology, volume 5, issue 6, 1995, Pages 364~369
Effect of two-phase culture on Mentha piperita cell growth and essential oil formation was investigated using shake flask and air-lift bioreactors. LiChroprep RP-B(RP-B) addition did not impair M. piperita cell growth, but resulted in stimulated formation of essential oils and increased ratios of extracellular oil to intracellular oil formation. However, the combined use of RP-B and chitosan elicitor was not synergistic. Volumetric productivity of essential oils in RP-B treated culture using cell-recycled air-lift bioreactor was
which was substantially higher than that obtainable from the control. Our results demonstrate the potential of a second phase to enhance overall productivity for M. piperita cell culture.
Cloning of Autonomously Replicating Sequence from Phaffia rhodzyma
Chun, Soon Bai ; Seung Hee Chun ;
Journal of Microbiology and Biotechnology, volume 5, issue 6, 1995, Pages 370~372
A Phaffia rhodozyma chromosomal fragment (approximately 3.8 kb) capable of functioning as an origin for the replication of a kanamycin resistance (
) plasmid in S. cerevisiae was isolated by the use of origin search plasmid, pHN134. In S. cerevisiae, transformation frequencies using the plasmid pHN134 containing an autonomously replicating sequence of P. rhodozyma was 450-580 CFU/
DNA. The stability of the recombinant plasmid were 16-19