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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Microbiology and Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 6, Issue 6 - Dec 1996
Volume 6, Issue 5 - Oct 1996
Volume 6, Issue 4 - Aug 1996
Volume 6, Issue 3 - Jun 1996
Volume 6, Issue 2 - Apr 1996
Volume 6, Issue 1 - Feb 1996
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Purification and Characterization of an Alkaline Protease from Bacillus licheniformis NS70
Kim, Young-Ok ; Lee, Jung-Kee ; Kim, Hyung-Kwoun ; Park, Young-Seo ; Oh, Tae-Kwang ;
Journal of Microbiology and Biotechnology, volume 6, issue 1, 1996, Pages 1~6
A bacterial strain NS70 producing an alkaline protease was isolated from soil samples taken near a hot spring and identified as Bacillus licheniformis by its morphological and physiological properties and cellular fatty acid analysis. The isolated alkaline protease was purified by ammonium sulfate fractionation, DEAE-, CM-, and Phenyl-Sepharose column chromatography. The molecular weight of the purified enzyme was estimated to be 32, 000 Da by sodium dodecylsulfate polyacrylamide gel electrophoresis. Its optimal pH and temperature for proteolytic activity against Hammarsten casein were 12 and
, respectively. The enzyme was stable at alkaline pH range from 6.0 to 12.0, and fairly stable up to
. The enzyme was inhibited by phenylmethylsulfonyl fluoride but not by EDTA and N-ethylmaleimide indicating that the enzyme is serine protease. Enzyme activity was markedly inhibited by
. Autolytic phenomena were observed on purified protease NS70 but autolysis was reduced by the addtion of
ion or bovine serum albumin.
Inhibition of Aminopeptidase N by Two Synthetic Tripeptides
Chung, Myung Chul ; Hyo Kon Chun ; Ho Jae Lee ; Choong Hwan Lee ; Su Il Kim ; Yung Hee Kho ;
Journal of Microbiology and Biotechnology, volume 6, issue 1, 1996, Pages 7~11
MR-387Al (ARPA-Val-Pro) and A2 (AHPA-Val-Hyp) were prepared as aminopeptidase N inhibitors through the synthesis of peptide MR-387A and B analogues which contained 3-amino-2-hydroxy-4-phenyl butanoic acid (ARPA) as a zinc-chelating moiety. They are competitive inhibitors of aminopeptidase N with inhibition constants(Ki) of 4.1
M, respectively. MR-387Al also strongly inhibited aminopeptidase B of human myelogenous leukemia K-562 cell with
M. Inhibitions of aminopeptidase N activity by ARPA-bearing inhibitors of various peptide chain lengths also have been studied.
values of AHPA-Val (bestatin), ARPA-Val-Pro (MR-387Al) and ARPA-Val-Pro-Leu (MR-387C) compared against porcine kidney aminopeptidase N were 20.1, 0.60 and 0.08
M, respectively. These results support that a multiple interaction between the
sites of aminopeptidase N and the
of the inhibitor plays a crucial role in stabilizing strongly the enzyme-inhibitor complex.
Identification and Production of Constitutive Chitosanase from Bacillus sp. HW-002
Lee, Hyean Woo ; Jong Whan Choi ; Dong Pyou Han ; Noo Woon Lee ; Sung Lim Park ; Dong Heui Yi ;
Journal of Microbiology and Biotechnology, volume 6, issue 1, 1996, Pages 12~18
A chitosanase-producing bacteria was isolated on chitosan agar plate from soil samples. The strain was spore-forming gram positive bacteria, catalase positive, and rod shape. The strain was identified as Bacillus cereus. The strain did not need an inducer for the synthesis of chitosanase. Chitosanase from Bacillus sp. HW-002 was constitutive enzyme. The optimal medium for the production of the enzyme was composed of 0.5
yeast extract-tryptone (1:1 w/w) mixture at pH 6.5. After Bacillus sp. HW-002 was cultivated at
for 32 h, maximal productivity was gained to be about 27, 200 U/l. Chitosanase from Bacillus sp. HW-002 was a mixed growth-linked metabolite.
Purification and Characteristics of Chitosanase from Bacillus sp. HW-002
Lee , Hyean-Woo ; Choi, Jong-Whan ; Han, Dong-Pyou ; Park, Myoung-Jin ; Lee, No-Woon ; Yi, Dong-Heui ;
Journal of Microbiology and Biotechnology, volume 6, issue 1, 1996, Pages 19~25
Chitosanase from Bacillus sp. HW-002 was purified with CM-cellulose column chromatography, and HPLC with DEAE- TSK gel and YMC-pack Diol 120. The purified enzyme appeared as a single band on SDS-polyacrylamide gel. The molecular weight of the enzyme was estimated to be about 46 kDa on SDS-polyacrylamide gel, and was estimated to be about 23 kDa by GFC. The optimal pH of chitosanolytic activity was about pH 5.5-6.0, and the purified enzyme was most stable at pH 5.0. The optimal temperature of chitosanolytic activity was
and the enzyme was stable at
for 1 h. Chitosan was the most favorable substrate among various
-glucan. UVmax of the purified enzyme was 195 nmand was not noted around 280 nm. The main product of enzyme reaction with chitosan was chitobiose.
Purification of the Three-subunit, Recombinant Bacillus pasteurii Urease Expressed in Escherichia coli
Lee, Ji Hyun ; Sang Dal Kim ; Mann Hyung Lee ;
Journal of Microbiology and Biotechnology, volume 6, issue 1, 1996, Pages 26~29
The genes coding for the urease of alkalophilic Bacillus pasteurii have been previously cloned and recently sequenced. (You, J. H., B. H. Song, J. H. Kim, M. H. Lee, and S. D. Kim (1995) Molecules and Cells 5, 359-369.) The recombinant Bacillus pasteurii urease expressed in an E. coli HB101 strain was purified 31.2 fold by using combinations of anion-exchange and hydrophobic chromatography followed by Mono-Q chromatography on a FPLC. In spite of the presence of three discrete structural peptide genes in the Bacillus pasteurii urease gene cluster, only one or two enzyme subunits have been observed to date. Here we report for the first time that the recombinant Bacillus pasteurii urease expressed in a E. coli strain consists of three distinct subunits. One large subunit was estimated to be of
=65, 200 and the two small-subunit peptides are of
=14, 500 and
=13, 700, respectively.
Effects of the Redox Potential of the Acidogenic Reactor on the Performance of a Two-Stage Methanogenic Reactor
Phae, Chae-Gun ; Lee, Wan-Kyu ; Kim, Byung-Hong ; Koh, Jong-Ho ; Kim, Sang-Won ;
Journal of Microbiology and Biotechnology, volume 6, issue 1, 1996, Pages 30~35
Distillery wastewater was used in a thermophilic laboratory-scale two stage anaerobic digester to test the effects of the redox potential of the first acidogenic reactor on the performance of the system. The digester consisted of first a acidogenic reactor and the an upflow anaerobic sludge blanket (UASB) reactor. The digestor was operated at a hydraulic retention time (HRT) of 48 h. Under these conditions, about 90% of the chemical oxygen demand as measured by the chromate method (
) was removed with a gas production yield of 0.4 l/g-COD removed. The redox potential of the acidogenic reactor was increased when the reactor was purged with nitrogen gas or agitation speed was increased. The increase in reduction potential was accompanied by an increase in acetate production and a decrease in butyrate formation. A similar trend was observed when a small amount of air was introduced into the acidogenic reactor. It is believed that the hydrogen partial pressure in the acidogenic reactor was decreased by the above mentioned treatments. The possible failure of anaerobic digestion processes due to over-loading could be avoided by the above mentioned treatments.
Enzymatic Sorbitol Production with Zymomonas mobilis Immobilized in k-Carrageenan
Jang, Ki-Hyo ; Jung, Sung-Je ; Chang, Hyun-Soo ; Chun, Uck-Han ;
Journal of Microbiology and Biotechnology, volume 6, issue 1, 1996, Pages 36~42
The production of sorbitol by permeabilized cells of Zymomonas mobilis immobilized in
-carrageenan was investigated. Cetyltrimethylammoniumbromide (CTAB) permeabilized cells were treated with glutaraldehyde prior to immobilization for cross-linking of enzymes, glucose-fructose oxidoreductase (GFOR) in cells. Rigidity of the immobilized beads was increased two-fold with 90
conversion efficiency by the additions of 40
(w/v) polyols (glycerol 25 g + propylene glycol 15 g) to 60
(w/v) distilled water containing 2.5
-carrageenan as a final concentration, prior to immobilization.
-Carrageenan beads entrapping permeabilized cells were dried to improve bead rigidity and storage stability. During s6mi-batch process for 72 h with dry beads, there was an improvement of the loss of enzyme activity (less than 10
). In batch process, the kinetic results of
value for the free cells, wet beads and dry
-carrageenan beads were 71.7, 72.4 and 116.7 g/l, respectively. Higher productivity was obtained with two-stage continuous packed bed reactors with both wet and dry
-carrageenan beads at 25.00 and 21.15 g/l/h, respectively, when measured at second stage.
Modulation of Phosphoenolpyruvate Metabolism of Anaerobiospirillum succiniciproducens ATCC 29305
Yoo, Jin Young ; J. Gregory Zeikus ;
Journal of Microbiology and Biotechnology, volume 6, issue 1, 1996, Pages 43~49
Modulation of the catabolic PEP-pathway of Anaerobiospirillum succiniciproducens was tried using some enzymatic inhibitors such as gases and chemicals in order to enhance succinic acid production. 10
CO increased the succinic acid/acetic acid (S/A) ratio but inhibited growth as well as production of succinic and acetic acid. Hydrogen gas also increased the S/A ratio and inhibited the synthesis of pyruvate: ferredoxin oxidoreductase when used in mixture with
, Catabolic repression by acetic, lactic and formic acid was not recognized and other modulators such as glyoxylate, pyruvate derivatives, arsenic salt, phosphate and sulfate were shown not to be effective. Magesium carbonate was shown effective for repressing acetate production. Palmitic acid, myristic acid and phenylalanine did not affect acetate production but carprylic acid completely inhibited growth.
Characteristics of the Alcoholic Milk Product Fermented by Lactococcus lactis subsp. lactis TA29 and Saccharomyces exiguus SK2
Hong, Seok-San ; Cha, Seong-Kwan ; Kim, Wang-June ; Koo, Young-Jo ;
Journal of Microbiology and Biotechnology, volume 6, issue 1, 1996, Pages 50~53
A cultured milk product was made by fennenting 10
reconstituted skim milk with Lactococcus lactis subsp. lactis TA29 and Saccharomyces exiguus SK2. L. lactis TA29 and S. exiguus SK2 grew up to 1.0
cfu/ml, respectively. After the fermentation 21
of lactose was hydrolyzed, pH was lowered to 4.2, and titratable acidity and alcohol concentration were increased to 0.96 and 0.023
, respectively. When the fermented milk was stored at
for 9 days, the viable cell counts for L. lactis TA29 and S. exiguus SK2 were
cfu/rnl, respectively. The alcoholic fermented milk prepared in this experiment was more inhibitory against some pathogenic bacteria including C. perfringens than commercial yoghurt products tested.
Construction of a Database for New Bioactive Compounds and Development of Search Systems
Park, Kie Jung ; Yong Ha Park ;
Journal of Microbiology and Biotechnology, volume 6, issue 1, 1996, Pages 54~59
In the research and development of bioactive compounds, determining whether a compound is novel is necessary at almost every stage. Fast and efficient determination can save money, time and effort, and thereby increase efficiency. Analysis and investigation of empirical results for previously determined compounds is also important in such research. The need to communicate research findings between workers is necessary. In effect, a systematic, centralized communication medium is required. Therefore, we have developed and constructed our own database and search systems. We have developed a search system on DOS and constructed a source file for our own database. To support multiple users, we have developed another specific and comprehensive search system, including powerful searching and output management features. The system has been developed to be simple and user-friendly, using the curses library of UNIX, while still allowing complicated queries to be performed easily with simple full-screen facilities. This UNIX version will be available for use by researchers on a computer network and is expected to make numerous contributions to basic research in universities. It will also have direct applications for institutes and industry.
Effect of Nitrogen Sources on the Production of Polyols by Aureobasidium pullulans
Yun, Jong-Won ; Kang, Sun-Chul ; Song, Seung-Koo ;
Journal of Microbiology and Biotechnology, volume 6, issue 1, 1996, Pages 60~62
Aureobasidium pullulans produced three kinds of extracellular polyols e.g. glycerol, mannitol, and sorbitol from either sucrose, glucose, fructose or mannose. Sorbitol was selectively produced when urea was used as a sole nitrogen source, and the amounts of sorbitol produced rapidly reached a plateau after 50 h where its maximum quantity was about 20 g/l with sucrose.
Evaluation of Optimum Conditions for Bacteriocin Production from Lactobacillus sp. JB-42 Isolated from Kimchi
Jo, Young Bae ; Kyung Mi Bae ; Sung Koo Kim ; Hong Ki Jun ;
Journal of Microbiology and Biotechnology, volume 6, issue 1, 1996, Pages 63~67
Bacteriocin-producing microorganism was isolated from Kimchi. The microorganism was identified as a Lactobacillus sp. and named Lactobacillus sp. JB-42. The optimum conditions for the bacteriocin production from the isolated microorganism were evaluated. For the maximum yield of bacteriocin from Lactobacillus sp. JB-42, the cell should be harvested at the early stationary phase and the temperature, pH and NaCl concentration should be
, pH 7 and without the addition of NaCl, respectively. Sucrose, glucose or fructose should be used as a carbon source and organic nitrogen such as tryptone should be used as a nitrogen source for the best yield. The production of bacteriocin was related to the cell growth of Lactobacillus sp. JB-42 indicating the role of Lactobacillus in the Kimchi fermentation process.
Isoprenoid Quinone Profiles of the Leclercia adecarboxylata KCTC
Shin, Yong Kook ; Jung Sook Lee ; Chang Ouk Chun ; Hong Joong Kim ; Yong Ha Park ;
Journal of Microbiology and Biotechnology, volume 6, issue 1, 1996, Pages 68~69
The isoprenoid quinone composition of Leclercia adecarboxylata KCTC
was determined by using high-performance liquid chromatography. L. adecarboxylata KCTC
are characterized by their production of both ubiquinone-7, ubiquinone-8 and menaquinone-8 as major quinones. It is clear that the analysis of isoprenoid quinone profiles provides a new criterion of great promise for identifying Leclercia strains.