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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Microbiology and Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 6, Issue 6 - Dec 1996
Volume 6, Issue 5 - Oct 1996
Volume 6, Issue 4 - Aug 1996
Volume 6, Issue 3 - Jun 1996
Volume 6, Issue 2 - Apr 1996
Volume 6, Issue 1 - Feb 1996
Selecting the target year
Purification, Characterization, and Comparison of Bacteriocins
Paik, Hyun-Dong ; Oh, Doo-Whan ;
Journal of Microbiology and Biotechnology, volume 6, issue 3, 1996, Pages 151~161
High-level Expression and Purification of Recombinant 4-Aminobutyrate Aminotransferases in Escherichia coli
Lee, Sung Gu ; Tae Jin Choi ; Young Tae Kim ;
Journal of Microbiology and Biotechnology, volume 6, issue 3, 1996, Pages 162~166
The protein coding sequence of the 4-aminobutyrate aminotransferase was amplified by polymerase chain reaction (PCR) from a previously cloned cDNA of pig brain using a pair of primers based on the published sequence. The amplified DNA was introduced into a T7 expression vector. Recombinant 4-aminobutyrate aminotransferases were overexpressed in Escherichia coli. The inclusion bodies were formed when enzyme was overexpressed. The unfolded, overproduced proteins were purified by chromatography with hydroxyapatite and refolded by a sequential dialysis method. The renatured 4-aminobutyrate aminotransferase regained the catalytic activity. However, the purified mutant protein did not show the catalytic function of 4-aminobutyrate aminotransferase.
Isolation and Characterization of Pseudomonas sp. P2 Degrading Polychlorinated Biphenyls (PCBs)
Kim, Jung Ho ; Sang Ki Choi ; Moon Ki Park ; Young Ho Kim ; Seung Kyo Suh ; Cheol Joo Woo ; Heui Dong park ;
Journal of Microbiology and Biotechnology, volume 6, issue 3, 1996, Pages 167~172
The bacterial strain P2 degrading polychlorinated biphenyls (PCBs) was isolated from the soil around the Shinchun stream in Taegu after enrichment culture in a media containing biphenyl as the sole carbon source. The isolate was identified as a strain of Pseudomonas sp. based on its morphological and physiological characteristics. The optimal conditions of initial pH of media and temperature for growth were 7.0 and
, respectively. Degradation of biphenyl and PCBs was confirmed by GC during the culture of Pseudomonas sp. P2 in a media containing them at a concentration of 500 mg/I. It was observed that Pseudomonas sp. P2 could degrade 97.0
of biphenyl and 60.0
of PCBs after 160 h culture.
Synergism among Endo-xylanase,
-Xylosidase, and Acetyl Xylan Esterase from Bacillus stearothermophilus
Suh, Jung-Han ; Choi, Yong-Jin ;
Journal of Microbiology and Biotechnology, volume 6, issue 3, 1996, Pages 173~178
Synergic effects among endo-xylanase,
-xylosidase, and acetyl xylan esterase of Bacillus stearothermophilus in the hydrolysis of xylan were studied by using birchwood, oat spelt, and acetylated xylan as substrates. Synergism between endo-xylanase and
-xylosidase was observed on all three substrates tested, indicating that
-xylosidase enhanced the production of xylose by relieving the end-product inhibition upon endo-xylanase conferred by xylooligomers. Endo-xylanase and
-xylosidase also showed synergism with acetyl xylan esterase in the hydrolysis of birchwood and acetylated xylan, while no synergic effect was detected in oat spelt xylan hydrolysis. Thus, the hydrolysis of xylan containing acetic acid side chains required the action of acetyl xylan esterase, which eliminated the steric hindrance of the side chains, leading to the better hydrolysis by endo-xylanase and
-xylosidase , and the acetyl xylan esterase activity was also enhanced by endo-xylanase and
-xylosidase for the latter enzymes provided acetyl xylan esterase with shorter xylan oligomers, the better substrate for the enzyme.
Synergic Effects among Endo-xylanase,
-L-Arabinofuranosidase from Bacillus stearothermophilus
Suh, Jung Han ; Ssang Goo Cho ; Yong Jin Choi ;
Journal of Microbiology and Biotechnology, volume 6, issue 3, 1996, Pages 179~183
Synergism among endo-xylanase,
-L-arabinofuranosidase from Bacillus stearothermophilus upon xylan hydrolysis was investigated by using birchwood, oat spelt, and arabinoxylan as substrates. Endo-xylanase and
-xylosidase showed the cooperative action on all three substrates tested, revealing the fact that
-xylosidase assists endo-xylanase action in xylan hydrolysis by relieving the endproduct inhibition upon endo-xylanase conferred by xylooligomers.
-L-Arabinofuranosidase also exhibited synergic effects with endo-xylanase and
-xylosidase on oat spelt and arabinoxylan, which contained significant amounts of arabinose side chains, whereas no synergism was detected on birchwood xylan which had only trace amounts of the side chain. Thus, the hydrolysis of xylan containing arabinose side chains required
-L-arabinofuranosidase as well as endo-xylanase and
-xylosidase for the better hydrolysis of the substrates, and these enzymes work cooperatively in order to maximize the extent and rate of xylan hydrolysis.
Purification and Characterization of Bioemulsifier Produced by Acinetobacter sp. BE-254
Kim, Soon-Han ; Lee, Jae-Dong ; Kim, Boo-Chul ; Lee, Tae-Ho ;
Journal of Microbiology and Biotechnology, volume 6, issue 3, 1996, Pages 184~188
The Acinetobacter sp. BE-254 isolated from soil sources produced a bioemulsifier in the medium supplemented with n-hexadecane. This bioemulsifier was purified by the procedures of fractionation (ammonium sulfate and chilled acetone), extraction by hexane, and column chromatography on silica gel 60. The results from various color reactions indicated that the bioemulsifier was a glycolipid. The purified emulsifier was very stable at pHs ranging from 4 to 10 and under heat treatment at
for 30 min. Emulsification activity was also hardly influenced by pH. The critical micelle concentration (CMC) and surface tension at the point (
) of the bioemulsifier were approximately 35 mg/l and 30 mN/m, respectively. The bioemulsifier showed a fairly good emulsification activity and stability in comparison with other commercial emulsifiers in the basic formula composed of emulsifier, oil, and water.
Removal of Endotoxins and Nucleic Acids Using Submicron-sized Polymeric Particles
Kim, Chan Wha ; Chokyun Rha ;
Journal of Microbiology and Biotechnology, volume 6, issue 3, 1996, Pages 189~193
Submicron-sized polymeric particles (SSPP) were used to remove nucleic acids and endotoxins from cell lysates. The positively charged SSPP selectively adsorb nucleic acids and endotoxins and form complexes with them. The complexes can be easily removed by sedimentation or centrifugation. The removal of nucleic acids and endotoxins using SSPP also can be accomplished in the presence of cell and cell debris. Therefore, nucleic acids and endotoxins can be removed in an initial step of the down-stream processes. In bakers yeast and E. coli lysate systems, the level of DNA could be reduced more than three orders of magnitudes and endotoxins more than seven orders of magnitudes concurrently willi the cell debris removal process using SSPP.
Volatile Components in the Soy Sauce Manufactured by Bacillus Species and Fused Yeast
Kim, Haeng-Ja ; Lee, Eun-Ju ; Shin, Ok-Sun ; Ji, Won-Dae ; Choi, Myeong-Rak ; Kim, Jong-Kyu ;
Journal of Microbiology and Biotechnology, volume 6, issue 3, 1996, Pages 194~201
To develop a method appropriate for mass production in a factory, we manufactured soy sauce with Bacillus species SSA3-2M1 and fused ST723-F31 at
with aeration of 1/3 vvm for 40 days. The flavor components extracted from the manufactured soy sauce were fractionated to neutral, acidic, basic and phenolic fraction and identified by GC-mass. Among the 60 kinds of identified flavor components, 16 and 23 components were detected in traditional Korean soy sauce and soybean paste, respectively. There were three peak regions that smelled like soy sauce with the GC sniffing test of flavor components and 2, 6-dimethyl pyrazine, benzaldehyde, 2-methoxy phenol, phenol and benzeneethanol which were identified as character impact compounds of traditional Korean soy sauce and soybean paste were identified in the region that smelled like soy sauce. It is therefore considered possible to achieve mass production of soy sauce with standard quality by Bacillus species SSA3-2M1 and fused ST723-F31 in the factory.
Taste Components of Soy Sauce Manufactured by Bacillus Species SSA3-2M1 and Fused ST723-F31
Kim, Haeng Ja ; Eun Ju Lee ; Ok Sun Shin ; Myeong Rak Choi ; Jong Kyu Kim ;
Journal of Microbiology and Biotechnology, volume 6, issue 3, 1996, Pages 202~208
In order to investigate fermenting conditions and the microorganisms necessary for factory production of traditional Korean soy sauce, we manufactured soy sauce made by Bacillus species SSA3-2M1 and fused ST723-F31 with aeration (1/30 vvm, 113 vvm and 2/3 vvm) at
for 40 days. This method was chosen to investigate the changes of dissolved oxygen, pH, cell number, flavor and the taste components during fermentation. When air was supplied (2/3 vvm) to the fermentor during fermentation, the flavor of the soy sauce and the composition of taste components (free amino acids, free sugars and organic acids) were similar to that of traditional Korean soy sauce after 22 days. The results of our experiments indicates that the mass production of traditional Korean soy sauce is possible using Bacillus species SSA3-2M1 and fused ST723-F31 given sufficient aeration.
Cryopreservation of Scutellaria baicalensis Cells by Two-step Cooling Method
Seo, Weon-Taek ; Kim, Suk-Weon ; Liu, Jang-Ryol ; Kim, Ik-Hwan ; Park, Young-Hoon ; Choe, Tae-Boo ;
Journal of Microbiology and Biotechnology, volume 6, issue 3, 1996, Pages 209~212
A two-step cooling technique has been developed for cryopreservation of suspension cultured Scutellaria baicalensis cells. Efficient regrowth of cryopreserved cells was obtained in cryoprotected cells with a mixture of 1.5 M glycerol and 0.4 M sucrose in Schenk and Hildebrandt medium without pretreatment in high osmotic medium. Optimum freezing conditions were found to be a cooling rate of
, and then retaining samples at
for 30 min prior to plunging into liquid nitrogen. A regrowth rate of approximately 95
was obtained after three month storage in liquid nitrogen. Callus cultures established from the cryopreserved cells were found to produce the same patterns of flavonoid accumulation and retain their baicalin producing activity.
Immunostimulating Activity and Characterization of Polysaccharides from Mycelium of Phellinus linteus
Lee, Jae Hoon ; Soo Muk Cho ; Kyung Sik Song ; Sang Bae Han ; Hwan Mook Kim ; Nam Doo Hong ; Ick Dong Yoo ;
Journal of Microbiology and Biotechnology, volume 6, issue 3, 1996, Pages 213~218
Hot-water extract, Fr. 1, of Phellinus linteus mycelium was fractionated into Fr. 2, 3, 4, and 5 by the difference of solubility in ethanol. The polysaccharide fractions were studied for their immunostimulating activity on in vitro T-independent polyc1onal antibody response to trinitrophenyl-haptened SRBC (sheep red blood cell). The Fr. 4 with the highest immunostimulating activity was subjected to DEAE-cellulose ion exchange chromatography and gave five fractions, 4-I, II, III, IV, and V. The in vitro immunostimulating assay of the five fractions showed that 4-I and 4-III had a similar activity to that of LPS but the other fractions had low activity. By analyses of chemical composition and HPLC, all fractions obtained were found to be heteropolysaccharide-protein complex. The molecular weights ranged from 9, 000 to 15, 000. Sugar analyses showed that glucose, galactose, mannose, arabinose, and xylose were main component. Uronic acid and amino sugar were also detected in the fractions. It should be noted that the molecular weight (15, 000) of 4-III was very small and the structure of 4-III may be different from the known immunostimulating branched
Linkage of the Kanamycin Resistance Gene with the Streptothricin Resistance Gene in Staphylococcus aureus SA2
Shin, Chul Kyo ; Sung Hwan Im ; Woo Koo Kim ; Kyung Bo Moon ;
Journal of Microbiology and Biotechnology, volume 6, issue 3, 1996, Pages 219~220
The pKH2 isolated from the multidrug-resistant Staphylococcus aureus SA2 is a 40.98-kb plasmid and mediates resistance to ampicillin, clindamycin, erythromycin, kanamycin, and streptomycin. The 3.4-kb HindIII fragment conferring kanamycin resistance was cloned from the pKH2 into pBluescriptII
and partial sequence determination of that fragment was carried out. Sequence analysis revealed that the kanamycin resistance gene which encoded aminoglycoside 3'-phosphotransferase was linked to the streptothricin resistance gene. But a nonsense mutation was found in the streptothricin resistance gene and this mutation resulted in a truncated protein of streptothricin acetyltransferase. Homology comparison with nucleotide sequence databases revealed that the 3.4-kb HindIII fragment of pKH2 had been derived not from S. aureus but from Gram-negative Campylobacter coli.
High Cell Density Cultivation of Pseudomonas putida BM01 Using Glucose
Kim, Guk Jin ; In Young Lee ; Dae Keon Choi ; Sung Chul Yoon ; Young Hoon Park ;
Journal of Microbiology and Biotechnology, volume 6, issue 3, 1996, Pages 221~224
Pseudomonas putida BM01 was grown efficiently on glucose as the sole carbon source with a supply of a nitrogen source in pH-stat mode using a low setpoint limit. A final cell concentration of 100 g/l was obtained in 30 h of fed-batch cultivation by controlling glucose concentration within the range of 5-20 g/l and maintaining dissolved oxygen tension above 10
saturation using pure oxygen. This high cell density culture technique is believed highly useful for the production of poly(3-hydroxyalkanoates) by this strain.