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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Microbiology and Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 7, Issue 6 - Dec 1997
Volume 7, Issue 5 - Oct 1997
Volume 7, Issue 4 - Aug 1997
Volume 7, Issue 3 - Jun 1997
Volume 7, Issue 2 - Apr 1997
Volume 7, Issue 1 - Feb 1997
Selecting the target year
A Strategy for Cheese Starter Culture Management in Australia
Lim, Sow-Tin ; Gaetan, K.Y. ; Bruinenberg, Paul-G. ; Powell, Ian-B. ;
Journal of Microbiology and Biotechnology, volume 7, issue 1, 1997, Pages 1~7
The efficient manufacture of fermented dairy products on an industrial scale requires a supply of reliable starter cultures with properties suited to desired product specifications. These cultures must be backed by relevant research and development activities. This article describes the issues involved in establishing a centre to provide starter culture R & D for a group of independent cheese manufacturing companies, and discusses a strategic approach to the management of starter cultures.
Overproduction of Escherichia coli D-Xylose Isomerase Using
Park, Heui-Dong ; Joo, Gil-Jae ; Rhee, In-Koo ;
Journal of Microbiology and Biotechnology, volume 7, issue 1, 1997, Pages 8~12
In order to overproduce D-xylose isomerase, the Escherichia coli D-xylose isomerase (D-xylose ketol-isomerase, EC 188.8.131.52) gene (xylA) was fused to
promoter. The promoterless xylA gene containing the ribosome binding site and coding region for D-xylose isomerase was cloned into a site 0.3 kb downstream from the
promoter on a high copy number plasmid. An octameric XbaI linker containing TAG amber codon was inserted between 33rd codon of
and the promoterless xylA gene. The resulting recombinant plasmid (designated as pPX152) was transformed into E. coli M5248 carrying a single copy of the temperature sensitive
gene on its chromosomal DNA. When temperature-induced, the transformants produced 15 times as much D-xylose isomerase as that of D-xylose-induced parent strain. The amount of overproduced D-xylose isomerase was found to be about 60% of total protein in cell-free extracts.
Role of Siderophores in Biocontrol of Fusarium solani and Enhanced Growth Response of Bean by Pseudomonas fluorescens GL20
Lim, Ho-Seong ; Kim, Sang-Dal ;
Journal of Microbiology and Biotechnology, volume 7, issue 1, 1997, Pages 13~20
Plant growth-promoting Psudomonas fluorescens GL20 was isolated from a ginseng rhizosphere on chrome azurol Sagar. P. fluorescens GL20 produced a large amount of hydoxamate siderophore in an iron-deficient medium. The siderophore showed significantly high specific activity of 20.2 unit. Using an in vitro antifungal test, P. fluorescens GL20 considerably suppressed growth of phytopathogenic fungus Fusarium solani, inhibiting spore germination and germ tube elongation. In pot trials of kidney beans with P. fluorescens GL20, disease incidence was remarkably reduced up to
compared with that of F. solani alone, and plant growth was also increased nearly 1.6 fold as compared to that of the untreated control, promoting elongation and development of the roots. These results indicate that the plant growth-promoting activity of P. fluorescens GL20 can play an important role in biological control of soil-borne plant disease in a rhizosphere, enhancing the growth of plants.
Investigation of the Relationship between Protein, Message and Inducer Concentrations in Recombinant E. coli Cells
Jorgensen, Lene ; Connor J. Thomas ; Brian K. Oneill ; Anton P.J. Middelbeg ;
Journal of Microbiology and Biotechnology, volume 7, issue 1, 1997, Pages 21~24
Chloramphenicol acetyl transferase (CAT) protein and mRNA levels in E. coli were determined following induction of a tac::cat construct by isopropyl-
-thiogalactopyranoside (IPTG). High cat mRNA levels did not directly reflect CAT protein levels, in either shakeflask experiments or fermentations. Furthermore, concentrations of IPTG resulting in the highest levels of expression of cat mRNA, were different to those resulting in highest levels of CAT protein. The data suggest that high transcriptional activities lead to limitations at the translational level.
Properties of the Fusants of Lactobacillus acidophilus 88 and Lactobacillus casei subsp. casei KCTC 1121
Jo, Young-Bae ; Heo, Kyeong ; Kim, Sung-Koo ; Baik, Hyung-Suk ; Jun, Hong-Ki ;
Journal of Microbiology and Biotechnology, volume 7, issue 1, 1997, Pages 25~31
Protoplast fusion between L. casei KCTC 1121 and L. acidophilus 88 was attempted to obtain improved strains. The fusants produced a bacteriocin against indicator strains, making a smaller inhibition zone compared to that of L. acidophilus 88. After culturing for 2 months on selective medium, the selected fusants were still stable without segregation. Fusants showed higher lipase activity compared to those of the two parent strains. Fusant No.4, 11, and 15 exhibited excellent lactic acid productivity. Fusant No.4 and 15 exhibited improved proteolysis ability compared to the two parent strains. Whereas L. casei possessed both
activities, and L. acidophilus 88 had only
activity, the fusants had both the intermediate enzyme activities. Cell size of the fusants was greater than that of the parents.
Identification of Adenosine Deaminase Inhibitor-producing Bacterium Isolated from Soil
SHIN, YONG KOOK ; YONG-HA PARK ; JAE-DONG LEE ; HONG-KI JUN ;
Journal of Microbiology and Biotechnology, volume 7, issue 1, 1997, Pages 32~36
An adenosine deaminase inhibitor-producing bacterium was isolated from soil. An isolate exhibiting high adenosine deaminase inhibitory activity, was designated J-89, and classified as a strain of Bacillus subtilis on the basis of its morphological, phenotypic characteristics, the menaquinone content and cellular fatty acid composition. To confirm the taxonomic position of the strain we need more information such as DNA-DNA homology and other chemotaxonomic characteristics. In this paper we provisionally named strain J-89 as Bacillus sp. J-89 pending further chemotaxonomic study and analysis of adenosine deaminase inhibitor.
Purification and Partial Characterization of Thermostable Carboxyl Esterase from Bacillus stearothermophilus L1
Kim, Hyung-Kwoun ; Park, Sun-Yang ; Oh, Tae-Kwang ;
Journal of Microbiology and Biotechnology, volume 7, issue 1, 1997, Pages 37~42
A bacterial strain L1 producing a thermostable esterase was isolated from soil taken near a hot spring and identified as Bacillus stearothermophilus by its microbiological properties. The isolated thermostable esterase was purified by ammonium sulfate fractionation, ion .exchange and hydrophobic interaction chromatographies. The molecular weight of the purified enzyme was estimated to be 50,000 by SDS-PAGE. Its optimum temperature and pH for hydrolytic activity against PNP caprylate were
and 9.0, respectively. The purified enzyme was stable up to
and at a broad pH range of 4.0-11.5 in the presence of bovine serum albumin. The enzyme was inhibited by phenylmethylsulfonyl fluoride and diethyl p-nitrophenyl phosphate, indicating the enzyme is a serine esterase. The enzyme obeyed Michaelis-Menten kinetics in the hydrolysis of PNPEs and had maximum activity for PNP caproate (
) among PNPEs (
Degradation of Chlorophenols and Phenol Mixtures by Cooperative Activities of Chlorophenol-degrading Strains
Bae, Hee-Sung ; Cho, Young-Gyun ; Lee, Sung-Taik ;
Journal of Microbiology and Biotechnology, volume 7, issue 1, 1997, Pages 43~48
Three strains capable of degrading a chlorophenol were isolated by selective enrichment from soils contaminated with industrial wastewater. A Pseudomonas solanacearum TCP114 could use 2,4,6-trichlorophenol (TCP) as sole carbon and energy source, while two strains of Pseudomonas testosteroni CPW301 and Arthrobacter ureafaciens CPR706 could use 4-CP. All isolates also grew well on phenol. The degradation of one component by a pure strain was strongly affected by the presence of other compounds in the medium, CPW301 and CPR706 entirely lost the ability to degrade 4-CP and phenol in the presence of TCP. TCP114 also lost the ability to degrade phenol when 4-CP was added to the culture medium. These restrictions on the degradability could be overcome by employing defined mixed cultures (TCP114 and one strain of 4-CP degrading strains). All three components were successfully degraded by defined mixed cultures through their cooperative activities. It was also demonstrated that defined mixed cultures could be immobilized by using calcium alginate for the semi-continuous degradation of the three component mixture. Immobilization could not only accelerate the degradation rate, but also allowed the reuse of the cell mass several times without loss of the cells' degrading capabilities.
Antifungal Activities of Magainin-2 Hybrid Peptides against Trichosporon beigelii
LEE, DONG GUN ; SONG YUB SHIN ; SUNG GU LEE ; KIL LYONG KIM ; MYUNG KYU LEE ; KYUNG SOO HAHM ;
Journal of Microbiology and Biotechnology, volume 7, issue 1, 1997, Pages 49~51
In order to obtain a hybrid synthetic peptide with a more potent antifungal activity than magainin-2 but without hemolytic activity, four hybrid peptides were designed from the sequences of magainin 2 and cecropin A and their antifungal activities against Trichosporon beigelii were investigated. The result showed that analogue 2 and 4 exhibited better antifungal activity against T. beigelii than magainin-2 but no hemolytic activities. The peptides, therefore, could be used as models for the development of potent antifungal peptides.
Immunostimulating Activity of Polysaccharides from Mycelia of Phellinus linteus Grown under Different Culture Conditions
Lee, Jae-Hoon ; Cho, Soo-Muk ; Kim, Hwan-Mook ; Hong, Nam-Doo ; Yoo, Ick-Dong ;
Journal of Microbiology and Biotechnology, volume 7, issue 1, 1997, Pages 52~55
Polysaccharides were extracted from mycelia of Phellinus linteus grown under different culture conditions. The in vitro immunostimulating activity was measured by plaque-forming cell (PFC) assay. The activity of the polysaccharides was different from that of mycelia from which was extracted. The number of PFC's ranged from 40 to 600 depending on the media. When P. linteus was cultured on a medium with mannose or starch as a sole carbon source, the fungus produced polysaccharide with the highest activity of 960 PFC. Activity was therefore increased by
compared with polysaccharide which was extracted from mycelia grown on medium with glucose. pH had little effect on the change in activity. All polysaccharides on media with different pH stimulated about 600 PFC. These results suggest that activity could be increased by polysaccharide modification through changes in physiological conditions.
Immunosuppressive Characteristics of Oligomycin Derivatives Produced by Streptomyces lydicus MCY-524
Lee, Sang-Yong ; Han, Sang-Bae ; Kim, Hang-Sub ; Kim, Young-Ho ; Kim, Hwan-Mook ; Kim, Chang-Jin ; Hong, Soon-Duck ; Lee, Jung-Joon ;
Journal of Microbiology and Biotechnology, volume 7, issue 1, 1997, Pages 56~61
A strain producing immunosuppressive substances was isolated from a soil in Cheju island. By morphological, cultural, and physiological studies, the strain was identified as Streptomyces lydicus MCY-524. Cultured broth was purified by silica gel, sephadex LH-20 and preparative HPLC and gave two immunosuppressive compounds, MCH-22 and MCH-32. They dramatically suppressed the B cell activation with lipopolysaccharide, T cell activation by mixed lymphocyte response, and primary T-dependent antibody response at a final concentration of 1
/ml. They also markedly suppressed the proliferation of lymphocytes induced by lipopolysaccharide, pokeweed mitogen, and concanavaline A at the same concentration. Their suppressive activities, which were comparable to those of cyclosporin A, suggested that they were potent and broad immunotoxic agents on the immune functions of murine lymphocytes.
Characterization of Ethanol Fermentation Using Alginate Immobilized Thermotolerant Yeast Cells
Sohn, Ho-Yong ; Park, Wan ; Jin, Ingnyol ; Seu, Jung-Hwn ;
Journal of Microbiology and Biotechnology, volume 7, issue 1, 1997, Pages 62~67
To enhance the hyperproductive and low energy-consuming ethanol fermentation rate, the thermotolerant yeast S. cerevisiae RA-74-2 cells were immobilized. An efficient immobilization condition was proved to be
(w/v) alginate solution, neutral pH and 20 h activation of beads. The fermentation characteristics and stability at various temperatures were examined as compared with free S. cerevisiae RA-74-2 cells. The immobilized cells had excellent fermentation rate at the range of pH 3-7 at 30-
glucose media. When the seed volume was adjusted to 0.12 (v/v) (6ml bead/50 ml medium),
(w/v) ethanol was produced during the first 34 hand
(w/v) ethanol [
(w/v) of theoretical yield] during the first 60 h in
glucose medium. In repetitive fermentation using a 2 litre fermentor, 5.79-
(w/v) ethanol [76-
(w/v) of theoretical yield] was produced during the 40-55 h in
glucose media. These data suggested the fact that alginate beads of thermotolerant S. cerevisiae RA-74-2 cells would contribute to economic and hyperproductive ethanol fermentation at high temperature.
Classification of Isolates Originating from Kimchi Using Carbon-source Utilization Patterns
LEE, JUNG-SOOK ; CHANG OUK CHUN ; MIN-CHUL JUNG ; WOO-SIK KIM ; HONG-JOONG KIM ; MARTIN HECTOR ; SAM-BONG KIM ; CHAN-SUN PARK ;
Journal of Microbiology and Biotechnology, volume 7, issue 1, 1997, Pages 68~74
One hundred and eighty two lactic acid bacteria, isolated mainly from kimchi, including reference strains were examined for their ability to utilize 95 carbon sources. The test strains were assigned to 5 major, 1 minor and 12 single-membered clusters based on the
, UPGMA algorithm (at similarity of
). These aggregate clusters were equivalent to the genus Leuconostoc (aggregate cluster M and N), the genus Lactobacillus (aggregate cluster Q and R), and the genera Lactobacillus and Leuconostoc (aggregate cluster O and P) according to the database of the Biolog system. This study demonstrates that rapid identification and classification of isolates originating from kimchi can be achieved on the basis of such carbon source utilization tests.
Biomass Production of Saccharomyces cerevisiae KFCC 10823 and Its Use in Preparation of Doenjang
Yoo, Jin-Young ; Kim, Hyeon-Gyu ; Kwon, Dong-Jin ;
Journal of Microbiology and Biotechnology, volume 7, issue 1, 1997, Pages 75~80
An ethanolic fermentation process was developed for preparing Doenjang with high ethanol. Higher and efficient viable cell production of salt-tolerant ethanolic yeast is a prerequisite for the successful commercial-scale process of ethanol production during Doenjang fermentation. Culture conditions of salt-tolerant yeast, S. cerevisiae KFCC 10823, was studied in terms of the effect of several environmental and nutritional factors. Viable cell numbers were the highest in a medium containing the following components per liter of water: soysauce, 300ml; dextrose, 50 g; beef extract, 5 g; yeast extract, 5 g;
, 5 g; NaCl, 50 g. The optimal culture conditions of S. cerevisiae KFCC 10823 were pH 5.5,
, 200 rpm and 0.5 vvm. Yeast viability during batch fermentation was gradually decreased to a level less than
after 35 hours. The maximum cell number was
cells/ml at the optimal condition. Doenjang prepared with ethanolic yeast was ripened after 45 days at
. This Doenjang contains 470 mg% of amino nitrogen and 2.5% ethanol. The shelf-life at
was theoretically estimated as 444 days.