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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Microbiology and Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 7, Issue 6 - Dec 1997
Volume 7, Issue 5 - Oct 1997
Volume 7, Issue 4 - Aug 1997
Volume 7, Issue 3 - Jun 1997
Volume 7, Issue 2 - Apr 1997
Volume 7, Issue 1 - Feb 1997
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Molecular Characterization of Intergeneric Hybrids between Trichoderma harzianum and Gliocladium virens
Shin, Pyung-Gyun ; Ryu, Jin-Chang ; Yoo, Young-Bok ; Jeong, Won-Hwa ; Cho, Moo-Je ;
Journal of Microbiology and Biotechnology, volume 7, issue 3, 1997, Pages 161~166
Nuclei were isolated from the protoplasts of Trichoderma harzianum T95 and treated with colchicine, a polyploid inducer. The nuclei were transferred into the protoplast of multi-auxotrophic Gliocladium virens G88 which cannot grow in minimal medium. The protoplast of G. virens G88 carrying the transferred nuclei were regenerated in a regeneration minimal medium containing
of chloroneb as a haploid inducer. Six intergeneric hybrids between G. virens and T. harzianum were isolated from the regeneration minimal medium. The hybrids could be classified into three types according to morphology, those with an isozyme pattern, those with an protein band and those with an randomly amplified polymorphic DNA(RAPD) pattern produced by random primers and repetitive sequences. The first group was identified to be a haploid recombinant, the second group a heterokaryon, and the third appeared to be petite.
Characteristics of ATPases Present in Everted Membrane Vesicles of Helicobacter pylori
Yun, Soon-Kyu ; Hwang, Se-Young ;
Journal of Microbiology and Biotechnology, volume 7, issue 3, 1997, Pages 167~173
Everted membrane vesicles of Helicobacter pylori were prepared and the membrane-resided ATPases were characterized. For comparison, Escherichia coli membrane ATPases and hog gastric mucosal H,K-ATPase were employed. ATPase assay revealed that the composite enzyme pool was relatively low in specific activities, below 1/10 times than that found in E. coli. According to their inhibitory specificities, most of the ATPase pool appeared to belong to the P-type ATPase, sensitive to vanadate but not to azide. The enzyme pool was extraordinarily resistant against treatment by N,N'-dicyclohexylcarbodiimide (DCCD). Certain monovalent cations, e.g.,
stimulated the whole enzyme pool only in the presence of
. On the contrary,
increased enzyme activity rather effectively without the aid of
. Under a defined condition employed, H. pylori cells could retain the membrane ATPase pool to the extent of
at pH 3.2. Moreover, its activity was most stable in acidic conditions (pH 5.4-6.4). However, cytoplasmic or peripheral ATPase pools were hardly detected under acidity (below pH 4.6).
Bifidogenic Effect of Glucooligosaccharide Prepared from Glucose by Extrusion Process
Ahn, Jun-Bae ; Hwang, Jae-Kwan ; Kim, Chong-Tai ; Lee, Ke-Ho ; Park, Jong-Hyun ;
Journal of Microbiology and Biotechnology, volume 7, issue 3, 1997, Pages 174~179
In order to investigate effect of glucooligosaccharide (GOS) prepared by extrusion process as a bifidogenic factor, cultivation of Bifidobacterium sp., Bacteroides fragilis and Clostridium perfringens was done and analyzed. B. fragilis and C. perfringens were able to utilize only 16% and 11% of the oligosaccharides in GOS, respectively, whilst Bifidobacterium sp. FBD-22 could utilize 38%. Especially, many kinds of oligo saccharides in GOS were able to be utilized selectively only by Bifidobacterium sp.. In case that GOS, as a carbon source, was used in the co-cultivation by Bifidobacterium sp., B. fragilis and C. perfringens, growth of Bifidobacterium sp. was not influenced by the existence of B. fragilis and C. perfringens. Bifidobacterium sp. showed advantage on carbon source competition for GOS with B. fragilis. Acetic acid, antimicrobial agent in the intestine, was produced two times more from GOS than glucose in co-cultures of three strains. Therefore, it is suggested that GOS can be a potent bifidogenic factor which proliferates the population of Bifidobacterium sp. and may finally improve the intestinal environments of human.
A Lipopeptide Biosurfactant Produced by Bacillus subtilis C9 Selected through the Oil Film-collapsing Assay
Kim, Hee-Sik ; Lee, Chang-Ho ; Suh, Hyun-Hyo ; Ahn, Keug-Hyun ; Oh, Hee-Mock ; Kwon, Gi-Seok ; Yang, Ji-Won ; Yoon, Byung-Dae ;
Journal of Microbiology and Biotechnology, volume 7, issue 3, 1997, Pages 180~188
Bacillus subtilis C9 was selected by measuring the oil film-collapsing activity and produced biosurfactant in a medium containing glucose as a sole carbon source. The biosurfactant emulsified hydrocarbons, vegetable oils and crude oil, and lowered the surface tension of culture broth to 28 dyne/cm. A biosurfactant, C9-BS produced by B. subtilis C9 was purified by ultrafiltration, extraction with chloroform and methanol, adsorption chromatography, and preparative reversed phase HPLC. Structural analyses, IR spectroscopy, FAB mass spectroscopy, amino acid composition, and NMR analyses, demonstrated that C9-BS was a lipopeptide comprising a fatty acid tail and peptide moiety. The lipophilic part consisting of
hydroxy fatty acid was linked to the hydrophilic peptide part, which contained seven amino acids (Glu-Leu-Leu-Val-Asp-Leu-Leu) with a lactone linkage.
Production of Monoclonal Antibody to the Infective Stage of Cryptosporidium Infection in AIDS Patients
Cho, Myung-Hwan ;
Journal of Microbiology and Biotechnology, volume 7, issue 3, 1997, Pages 189~193
Cryptosporidium parvum has been recognized as a significant cause of life-threatening diarrhea in Acquired ImmunoDeficiency Syndrome (AIDS) patients. Clinical diagnosis of cryptosporidial infections has been primarily based on the detection of infective stage, oocysts, in stools. Anti-Cryptosporidium oocyst monoclonal antibody (mAb), IgG2a, recognizing an antigen of 97 kDa was generated to be used for diagnosis of Cryptosporidium infection in AIDS patients using an immunofluorecence. It appeared to react with the surface antigens. Transmission electron micrographs of the infective stage of Cryptosporidium recognized by this mAb demonstrated sporolulated oocysts, which measure
, and sporozoites excysting from oocysts.
Immunochemical Reactivity of Polyclonal Antibody against Ampicillin Acylase of Xanthomonas citri
Kim, Mee-Jeong ; Kim, Su-Won ; Bang, Jeong-Hee ; Nam, Doo-Hyun ;
Journal of Microbiology and Biotechnology, volume 7, issue 3, 1997, Pages 194~196
Using the polyclonal antibody for Xanthomonas citri ampicillin acylase raised in Pseudomonas-free Balb/c mice, the immunochemical similarity of several types of penicillin acylases including Erwinia aroideae penicillin V acylase, Escherichia coli penicillin G acylase, Pseudomonas melanogenum and Acetobacter turbidans ampicillin acylases, and Pseudomonas cephalosporin acylase was examined. Among tested, only P. melanogenum ampicillin acylase showed the cross-reactivity with the antibody.
Production of Indigo and Indirubin by Escherichia coli Containing a Phenol Hydroxylase Gene of Bacillus stearothermophilus
Kim, In-Cheol ; Chang, Hae-Choon ; Oriel, Patrick ;
Journal of Microbiology and Biotechnology, volume 7, issue 3, 1997, Pages 197~199
Escherichia coli recombinants containing the cloned phenol hydroxylase gene of Bacillus stearothermophilus BR219 were shown to produce both indigo and its structural isomer indirubin during culture on LB broth. The ratio of indirubin/indigo was highest under conditions of prolonged culture and reduced culture oxygenation.
Nutritional Flexibility of Oligotrophic and Copiotrophic Bacteria Isolated from Deionzed-ultrapure Water Made by High-purity Water Manufacturing System in A Semiconductor Manufacturing Company
Kim, In-Seop ; Kim, Seung-Eun ; Hwang, Jung-Sung ;
Journal of Microbiology and Biotechnology, volume 7, issue 3, 1997, Pages 200~203
Bacteria isolated from ultrapure water made by a high-purity water manufacturing system in a semiconductor manufacturing plant were classified into two groups which either grew in diluted nutrient broth medium (oligotrophic bacteria) or could not grow (copiotrophic bacteria). The nutritional flexibility of oligotrophic and copiotrophic bacteria was investigated. The oligotrophic bacteria were shown to be able to utilize a significantly broader range of organic substrates than the copiotrophic bateria. This finding substantiates the hypothesis that nutritional flexibility is adaptive for oligotrophic bacteria.
Simultaneous Formation of Fructosyltransferase and Glucosyltransferase in Aureobasidium pullulans
Yun, Jong-Won ; Kim, Dong-Hyun ; Moon, Hye-Yeon ; Song, ChiiI-Hyun ; Song, Seung-Koo ;
Journal of Microbiology and Biotechnology, volume 7, issue 3, 1997, Pages 204~208
Aureobasidium puliulans possesses the capacity for simultaneous formation of fructosyltransferase and glucosyltransferase in various sugar media including sucrose, maltose, glucose and fructose. Among them, sucrose (300 g/1) was the most suitable carbon source for fructosyltransferase production, while fructose (100 g/1) gave the maximal production of glucosyltransferase. There existed a critical concentration for the optimal formation of enzymes in sucrose, glucose and fructose media. By contrast, no effect of maltose concentrations up to 300 g/1 was observed. The specific activity of the glucosyltransferase on maltose medium was highest during the early period of fetmentation, after which a sharp decrease occurred, whereas fructosyltransferase activity on sucrose medium maintained a nearly constant rate for a given culture period. Concomitant production of fructosyltransferase and glucosyltransferase was investigated with different combinations of lower concentrations of sucrose and maltose. Maltose supplementation in sucrose media and sucrose addition to maltose media enhanced the activity ratios of fructosyltransferase to glucosyltransferase as compared to that of non-supplemented media. Several polymers and surfactants were added in an attempt to enhance enzyme production, and supplementation of polyoxyethylene-sorbitan monolaurate (Tween 20) promoted fructosyltransferase production by 20%.
Quantitative Assay of Bioemulsifier by Turbidometric Method
Jeong, Yong-Leen ; Park, Oh-Jin ; Yoon, Byung-Dae ; Yang, Ji-Won ;
Journal of Microbiology and Biotechnology, volume 7, issue 3, 1997, Pages 209~211
A quantitative method for assaying bioemulsifiers in culture broth was developed and applied to cultivation of Pseudomonas aeruginosa YPJ80. SED(Standard Emulsification Dilution), an indirect measure of bioemulsifier concentration, was proposed. Production of bioemulsifier and rhamnolipid reached their maximum simultaneously. However, the bioemulsifier/rhamnolipid ratio decreased with cultivation time. This indicates the presence of another bioemulsifier other than rhamnolipid. The bioemulsifier seems to be protein-like activator which showed emulsification activity in addition to rhamnolipid.
A New Alkalophilic Bacterium Producing Ethylene
Bae, Moo ; Kim, Mi-Ye ;
Journal of Microbiology and Biotechnology, volume 7, issue 3, 1997, Pages 212~214
A new isolate, Bacillus sp. ALK-7 can synthesize ethylene from l-aminocyclopropane-l-carboxylic acid (ACC) as well as from methionine. The ACC has only been recognized as a key intermediate found in the metabolic pathway leading to ethylene formation in various plants. The efficiency of ethylene formation from the ACC by Bacillus sp. ALK-7 was about 2 times as high as that from the methionine. The reaction from ACC to ethylene formation was also shown to be mediated by the cell-free extracts of Bacillus sp. ALK-7.