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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biochemistry and Molecular Biology
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Volume & Issues
Volume 41, Issue 12 - Dec 2008
Volume 41, Issue 11 - Nov 2008
Volume 41, Issue 10 - Oct 2008
Volume 41, Issue 9 - Sep 2008
Volume 41, Issue 8 - Aug 2008
Volume 41, Issue 7 - Jul 2008
Volume 41, Issue 6 - Jun 2008
Volume 41, Issue 5 - May 2008
Volume 41, Issue 4 - Apr 2008
Volume 41, Issue 3 - Mar 2008
Volume 41, Issue 2 - Feb 2008
Volume 41, Issue 1 - Jan 2008
Selecting the target year
Multiple roles of phosphoinositide-specific phospholipase C isozymes
Suh, Pann-Ghill ; Park, Jae-Il ; Manzoli, Lucia ; Cocco, Lucio ; Peak, Joanna C. ; Katan, Matilda ; Fukami, Kiyoko ; Kataoka, Tohru ; Yun, Sang-Uk ; Ryu, Sung-Ho ;
BMB Reports , volume 41, issue 6, 2008, Pages 415~434
DOI : 10.5483/BMBRep.2008.41.6.415
Phosphoinositide-specific phospholipase C is an effector molecule in the signal transduction process. It generates two second messengers, inositol-1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. Currently, thirteen mammal PLC isozymes have been identified, and they are divided into six groups: PLC-
. Sequence analysis studies demonstrated that each isozyme has more than one alternative splicing variant. PLC isozymes contain the X and Y domains that are responsible for catalytic activity. Several other domains including the PH domain, the C2 domain and EF hand motifs are involved in various biological functions of PLC isozymes as signaling proteins. The distribution of PLC isozymes is tissue and organ specific. Recent studies on isolated cells and knockout mice depleted of PLC isozymes have revealed their distinct phenotypes. Given the specificity in distribution and cellular localization, it is clear that each PLC isozyme bears a unique function in the modulation of physiological responses. In this review, we discuss the structural organization, enzymatic properties and molecular diversity of PLC splicing variants and study functional and physiological roles of each isozyme.
Structures of proteases for ubiqutin and ubiquitin-like modifiers
Ha, Byung-Hak ; Kim, Eunice Eun-Kyeong ;
BMB Reports , volume 41, issue 6, 2008, Pages 435~443
DOI : 10.5483/BMBRep.2008.41.6.435
Post-translational modifiers can alter the function of proteins in many different ways. The conjugation of ubiquitin (Ub) and ubiqutin-like modifiers (Ubls) to proteins has been shown to be especially crucial in regulating a variety of cellular processes including the cell cycle, growth control, quality control, localization and many more. It is a highly dynamic process and involves a number of enzymes called E1, E2 and E3. Ub and Ubls are removed from the target proteins by deubiquitinating enzymes (DUBs) or Ubl-specific proteases (ULPs), thereby deconjugation can act as an additional level of control over the ubiquitin-conjugation system. In addition, DUBs and ULPs are responsible for activating Ub and Ubls from their inactive corresponding precursor forms. Here we review recent progress in molecular details of these deconjugating enzymes of Ubls.
Modeled structure of trypanothione reductase of Leishmania infantum
Singh, Bishal K. ; Sarkar, Nandini ; Jagannadham, M.V. ; Dubey, Vikash K. ;
BMB Reports , volume 41, issue 6, 2008, Pages 444~447
DOI : 10.5483/BMBRep.2008.41.6.444
Trypanothione reductase is an important target enzyme for structure-based drug design against Leishmania. We used homology modeling to construct a three-dimensional structure of the trypanothione reductase (TR) of Leishmania infantum. The structure shows acceptable Ramachandran statistics and a remarkably different active site from glutathione reductase(GR). Thus, a specific inhibitor against TR can be designed without interfering with host (human) GR activity.
Characterization of two plasma membrane protein 3 genes (PutPMP3) from the alkali grass, Puccinellia tenuiflora, and functional comparison of the rice homologues, OsLti6a/b from rice
Chang-Qing, Zhang ; Shunsaku, Nishiuchi ; Shenkui, Liu ; Tetsuo, Takano ;
BMB Reports , volume 41, issue 6, 2008, Pages 448~454
DOI : 10.5483/BMBRep.2008.41.6.448
Two full-length cDNAs, PutPMP3-1 and PutPMP3-2, encoding PMP3 family proteins were isolated from Puccinellia tenuiflora, a monocotyledonous halophyte. Expression of both genes was induced by low temperature, salt stress, dehydration, ABA, and
. Transcripts of PutPMP3-2 were more strongly induced by these stresses relative to those of PutPMP3-1, particularly under low temperature and dehydration conditions. Expression of PutPMP3-1 and PutPMP3-2 in yeast mutants lacking the PMP3 gene can functionally complement the membrane hyper-polarization and salt sensitivity phenotypes resulting from PMP3 deletion. To compare the functions of PutPMP3-1 and PutPMP3-2, the orthologous genes in rice (OsLti6a and OsLti6b) were isolated. Both OsLti6a and OsLti6b could functionally complement the loss of PMP3 in yeast. PutPMP3-2 and OsLti6a were more effective in reversing membrane hyperpolarization than PutPMP3-1 and OsLti6b. However, the four yeast transformants each showed similar levels of salt tolerance. These results imply that these PMP3 family members don't function identically under different stress tolerance conditions.
Novel calcineurin interacting protein-2: the functional characterization of CNP-2 in Caenorhabditis elegans
Xianglan, Cai ; Ko, Kyung-Min ; Singaravelu, Gunasekaran ; Ahnn, Joo-Hong ;
BMB Reports , volume 41, issue 6, 2008, Pages 455~460
DOI : 10.5483/BMBRep.2008.41.6.455
Calcineurin (Cn) is a serine/threonine phosphatase implicated in a wide variety of biological responses. To identify proteins that mediate Cn signaling pathway effects, we used yeast two-hybrid assays to screen for Cn interacting proteins, discovering a protein encoded by the gene, cnp-2 (Y46G5A.10). Utilizing serially deleted forms of Cn as baits, we demonstrated that the catalytic domain of Cn (TAX-6) binds with CNP-2, and this physical interaction was able to be reconstituted in vitro, supporting our yeast two-hybrid results. cnp-2 is a nematode-specific novel gene found in C. elegans as well as its closest relative, C. briggsae. CNP-2 was strongly expressed in the intestine of C. elegans. To study the function of cnp-2, we performed cnp-2 RNAi knock-down and characterized phenotypes associated with Cn mutants. However, no gross defects were revealed in these RNAi experiments. CNP-2 was proven to be a Cn binding protein; however, its role remains to be elucidated.
IL-18 gene expression pattern in exogenously treated AML cells
Seo, Min-Ji ; Park, Min-Ha ; Yook, Yeon-Joo ; Kwon, Young-Sook ; Suh, Young-Ju ; Kim, Min-Jung ; Cho, Dae-Ho ; Park, Jong-Hoon ;
BMB Reports , volume 41, issue 6, 2008, Pages 461~465
DOI : 10.5483/BMBRep.2008.41.6.461
IL-18 production may enhance immune system defense against KG-1 cells ; NB4 cells, which are associated with good prognosis, do not produce IL-18. In this study, we treated KG-1 cells with IL-18 and used microarray technology to assess subsequent effects on gene expression. In UniGene-array of 7488 human genes, expression of 57 genes, including stress related genes, increased at least 2-fold, whereas expression of 48 genes decreased at least 2-fold. Following exogenous exposure of KG-1 cells to IL-18, expression of CRYGC,
and NACA gene were monitored. The latter is a transcriptional coactivator potentiating c-Jun-mediated transcription.
is an inhibitor of
, and affects growth regulation, apoptosis and hypoxic stress. Studies, such as this one, are beginning to clarify the differences between cells associated with good and bad cancer prognoses, which may ultimately assist in medical treatment for acute myeloid leukemia.
Minimizing a QTL region for intramuscular fat content by characterizing the porcine Phosphodiesterase 4B (PDE4B) gene
Kim, Jae-Hwan ; Ovilo, Cristina ; Park, Eung-Woo ; Fernndez, Almudena ; Lee, Jun-Heon ; Jeon, Jin-Tae ; Lee, Jung-Gyu ;
BMB Reports , volume 41, issue 6, 2008, Pages 466~471
DOI : 10.5483/BMBRep.2008.41.6.466
Three isoforms of pig PDE4B were cloned and classified as two forms: PDE4B1 and PDE4B3, which contain UCR1 and UCR2; and PDE4B2, which contains only UCR2. The amino acid sequences of each isoform showed good conservation in human and rat. PDE4B2 is expressed in a wide range of tissues, but PDE4B1 and PDE4B3 are not. Using an informative SNP for the Iberian x Landrace intercross detected from intron 12, a linkage map was constructed. The location of PDE4B was estimated at 123.6 cM outside of the QTL-CI (124-128 cM) for IMF. However, the QTL-CI for IMF was reconfirmed with high significance, and its position was narrowed down to an interval of 4 cM (the region defined by markers PDE4B and SW1881). Using radiation hybrid mapping, LEPR, LEPROT, DNAJC6, AK3L1 and AK3L2 were selected as positional and/or functional candidates related to the QTL.
Metabolic engineering of aliphatic glucosinolates in Chinese cabbage plants expressing Arabidopsis MAM1, CYP79F1, and CYP83A1
Zang, Yun-Xiang ; Kim, Jong-Hoon ; Park, Young-Doo ; Kim, Doo-Hwan ; Hong, Seung-Beom ;
BMB Reports , volume 41, issue 6, 2008, Pages 472~478
DOI : 10.5483/BMBRep.2008.41.6.472
Three Arabidopsis cDNAs, MAM1, CYP79F1, and CYP83A1, required for aliphatic glucosinolate biosynthesis were introduced into Chinese cabbage by Agrobacterium tumefaciens-mediated transformation. The transgenic lines overexpressing MAM1 or CYP83A1 showed wild-type phenotypes. However, all the lines overexpressing CYP79F1 displayed phenotypes different from wild type with respect to the stem thickness as well as leaf width and shape. Glucosinolate contents of the transgenic plants were compared with those of wild type. In the MAM1 line M1-1, accumulation of aliphatic glucosinolates gluconapin and glucobrassicanapin significantly increased. In the CYP83A1 line A1-1, all the aliphatic glucosinolate levels were increased, and the levels of gluconapin and glucobrassicanapin were elevated by 4.5 and 2 fold, respectively. The three CYP79F1 transgenic lines exhibited dissimilar glucosinolate profiles. The F1-1 line accumulated higher levels of gluconapoleiferin, glucobrassicin, and 4-methoxy glucobrassicin. However, F1-2 and F1-3 lines demonstrated a decrease in the levels of gluconapin and glucobrassicanapin and an increased level of 4-hydroxy glucobrassicin.
Transient activation of the MAP kinase signaling pathway by the forward signaling of EphA4 in PC12 cells
Shin, Jong-Dae ; Gu, Chang-Kyu ; Kim, Ji-Eun ; Park, Soo-Chul ;
BMB Reports , volume 41, issue 6, 2008, Pages 479~484
DOI : 10.5483/BMBRep.2008.41.6.479
In the present study, we demonstrate that ephrin-A5 is able to induce a transient increase of MAP kinase activity in PC12 cells. However, the effects of ephrin-A5 on the MAP kinase signaling pathway are about three-fold less than that of EGF. In addition, we demonstrate that EphA4 is the only Eph member expressed in PC12 cells, and that tyrosine phosphorylation induced by ephrin-A5 treatment is consistent with the magnitude and longevity of MAP kinase activation. Experiments using the Ras dominant negative mutant N17Ras reveal that Ras plays a pivotal role in ephrin-A5-induced MAP kinase activation in PC12 cells. Importantly, we found that the EphA4 receptor is rapidly internalized by endocytosis upon engagement of ephrin-A5, leading to a subsequent reduction in the MAP kinase activation. Together, these data suggest a novel regulatory mechanism of differential Ras-MAP kinase signaling kineticsexhibited by the forward signaling of EphA4 in PC12 cells.