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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biochemistry and Molecular Biology
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Volume & Issues
Volume 42, Issue 12 - Dec 2009
Volume 42, Issue 11 - Nov 2009
Volume 42, Issue 10 - Oct 2009
Volume 42, Issue 9 - Sep 2009
Volume 42, Issue 8 - Aug 2009
Volume 42, Issue 7 - Jul 2009
Volume 42, Issue 6 - Jun 2009
Volume 42, Issue 5 - May 2009
Volume 42, Issue 4 - Apr 2009
Volume 42, Issue 3 - Mar 2009
Volume 42, Issue 2 - Feb 2009
Volume 42, Issue 1 - Jan 2009
Selecting the target year
Biomedicinal implications of high-density lipoprotein: its composition, structure, functions, and clinical applications
Cho, Kyung-Hyun ;
BMB Reports , volume 42, issue 7, 2009, Pages 393~400
DOI : 10.5483/BMBRep.2009.42.7.393
High-density lipoprotein (HDL) is a proven biomarker for the monitoring of changes in antioxidant and anti-inflammation capability of body fluids. The beneficial virtues of HDL are highly dependent on its lipids and protein compositions, and their ratios. In normal state, the HDL particle is enriched with lipids and several HDL-associated enzymes, which are responsible for its antioxidant activity. Lower HDL-cholesterol levels (<40 mg/dL) have been recognized as an independent risk factor for coronary artery disease, as well as being a known component of metabolic syndrome. Functional and structural changes of HDL have been recognized as factors pivotal to the evaluation of HDL-quality. In this review, I have elected to focus on the functional and structural correlations of HDL and the roles of HDL-associated apolipoproteins and enzymes. Recent clinical applications of HDL have also been reviewed, particularly the therapeutic targeting of HDL metabolism and reconstituted HDL; these techniques represent promising emerging strategies for the treatment of cardiovascular disease, for drug or gene therapy.
Targeted chiral lipidomics analysis of bioactive eicosanoid lipids in cellular systems
Lee, Seon-Hwa ; Blair, Ian A. ;
BMB Reports , volume 42, issue 7, 2009, Pages 401~410
DOI : 10.5483/BMBRep.2009.42.7.401
We have developed a targeted lipidomics approach that makes it possible to directly analyze chiral eicosanoid lipids generated in cellular systems. The eicosanoids, including prostaglandins (PGs), thromboxanes (TXs), leukotrienes (LTs) and alcohols (HETEs), have been implicated as potent lipid mediators of various biological processes. Enzymatic formations of eicosanoids are regioselective and enantioselective, whereas reactive oxygen species (ROS)-mediated formation proceeds with no stereo-selectivity. To distinguish between enzymatic and non-enzymatic pathways of eicosanoid formation, it is necessary to resolve enantiomeric forms as well as regioisomers. High sensitivity is also required to analyze the eicosanoid lipids that are usually present as trace amounts (pM level) in biological fluids. A discovery of liquid chromatography-electron capture atmospheric pressure chemical ionization/mass spectrometry (LC-ECAPCI/MS) allows us to couple normal phase chiral chromatography without loss of sensitivity. Analytical specificity was obtained by the use of collision-induced dissociation (CID) and tandem MS (MS/MS). With combination of stable isotope dilution methodology, complex mixtures of regioisomeric and enantiomeric eicosanoids have been resolved and quantified in biological samples with high sensitivity and specificity. Targeted chiral lipidomics profiles of bioactive eicosanoid lipids obtained from various cell systems and their biological implications have been discussed.
31-binding motifs in the intrinsically unfolded transcriptional activation domain of VP16
Kim, Do-Hyoung ; Lee, Si-Hyung ; Nam, Ki-Hoon ; Chi, Seung-Wook ; Chang, Ik-Soo ; Han, Kyou-Hoon ;
BMB Reports , volume 42, issue 7, 2009, Pages 411~417
DOI : 10.5483/BMBRep.2009.42.7.411
Transcriptional activation domain (TAD) in virion protein 16 (VP16) of herpes simplex virus does not have any globular structure, yet exhibits a potent transcriptional activity. In order to probe the structural basis for the transcriptional activity of VP16 TAD, we have used NMR spectroscopy to investigate its detailed structural features. Results show that an unbound VP16 TAD is not merely "unstructured" but contains four short motifs (residues 424-433, 442-446, 465-467 and 472-479) with transient structural order. Pre-structured motifs in other intrinsically unfolded proteins (IUPs) were shown to be critically involved in target protein binding. The 472-479 motif was previously shown to bind to
, whereas the
-binding ability of other motifs found in this study has not been addressed. The VP16 TAD represents another IUP whose pre-structured motifs mediate promiscuous binding to various target proteins.
Effects of epitope sequence tandem repeat and proline incorporation on polyclonal antibody production against cytochrome 1A2 and 3A4
Ahn, Tae-Ho ; Yun, Chul-Ho ;
BMB Reports , volume 42, issue 7, 2009, Pages 418~420
DOI : 10.5483/BMBRep.2009.42.7.418
We describe a method for producing polyclonal antibodies against peptide antigen cytochrome P450 1A2 and 3A4 using a tandem repeat of the epitope region and incorporation of proline residue between the repeated sequences. An ELISA assay revealed more efficient generation of polyclonal antibodies to tandem repeat peptide antigens than mono-epitope peptides. The incorporation of proline residues further stimulated antibody production.
Transcriptional activation of an anti-oxidant mouse Pon2 gene by dexamethasone
Lim, Ji-Ae ; Kim, Sang-Hoon ;
BMB Reports , volume 42, issue 7, 2009, Pages 421~426
DOI : 10.5483/BMBRep.2009.42.7.421
Glucocorticoids regulate multiple physiological processes such as metabolic homeostasis and immune response. Mouse Pon2 (mPon2) acts as an antioxidant to reduce cellular oxidative stress in cells. In this present study, we investigated the transcriptional regulation of mPon2 by glucocorticoids. In the presence of glucocorticoid analogue dexamethasone, the expression of mPon2 mRNA in cells was increased, whereas the expression was inhibited by a transcription inhibitor actinomycin D. Glucocorticoid receptors bound to the putative glucocorticoid response elements located between -593 bp and -575 bp of the mPon2 promoter. Transcriptional activity was completely blocked when the putative element was mutated. Taken together, these results suggest that the expression of the mPon2 gene is directly regulated by glucocorticoid-glucocorticoid receptor complexes.
Static tensional forces increase osteogenic gene expression in three-dimensional periodontal ligament cell culture
Ku, Seung-Jun ; Chang, Young-Il ; Chae, Chang-Hoon ; Kim, Seong-Gon ; Park, Young-Wook ; Jung, Youn-Kwan ; Choi, Je-Yong ;
BMB Reports , volume 42, issue 7, 2009, Pages 427~432
DOI : 10.5483/BMBRep.2009.42.7.427
Orthodontic tooth movement results from the combinational process of both bone resorption and formation in the compressive and tension sides, respectively. However, the genes responsible for new bone formation in tension sides have not been determined. In this study, we used DNA microarray and real-time RT-PCR to identify genes in human periodontal ligament (PDL) cells that undergo significant changes in expression in response to static tensional forces (2 or 12 hours). The genes found were alkaline phospatase (ALP), matrix metalloproteinases (MMPs), vascular endothelial growth factor (VEGF), and several collagen genes. Furthermore, an ELISA evaluating the expression of VEGF, type IV collagen and MMP-2 found levels significantly increased after 24 and 72 hours (P < 0.05). ALP activity was also increased after 24 hours (P < 0.05). Collectively, we found the genes up-regulated in our study by the static tensional force are related to osteogenic processes such as matrix synthesis and angiogenesis.
Proteome analysis of the m. longissimus dorsi between fattening stages in Hanwoo steer
Kim, Nam-Kuk ; Lee, Seung-Hwan ; Cho, Yong-Min ; Son, Eun-Suk ; Kim, Kyung-Yun ; Lee, Chang-Soo ; Yoon, Du-Hak ; Im, Seok-Ki ; Oh, Sung-Jong ; Park, Eung-Woo ;
BMB Reports , volume 42, issue 7, 2009, Pages 433~438
DOI : 10.5483/BMBRep.2009.42.7.433
The objective of this study was to identify proteins in the m. longissimus dorsi between early (12 months of age) and late (27 months of age) fattening stages of Hanwoo (Korean cattle) steers. Using two-dimensional electrophoresis and mass spectrometry, 8 proteins of 11 differentially expressed spots between the 12 and 27 month age groups were identified in the loin muscle. Among those that were differentially expressed, zinc finger 323 and myosin light chain were highly expressed in late-fattening stage, and two catabolic enzymes, triosephosphate isomerase (TPI) and succinate dehydrogenase (SDH) were expressed more in the early versus the late-fattening stage. In particular, the quantification of TPI and SDH by immunoblotting correlated well with fat content. Our data suggested that TPI and SDH are potential candidates as markers and their identification provides new insight into the molecular mechanisms and pathways associated with intramuscular fat contents of bovine skeletal muscle.
Differential expression of a poplar SK
-type dehydrin gene in response to various stresses
Bae, Eun-Kyung ; Lee, Hyo-Shin ; Lee, Jae-Soon ; Noh, Eun-Woon ;
BMB Reports , volume 42, issue 7, 2009, Pages 439~443
DOI : 10.5483/BMBRep.2009.42.7.439
Dehydrins are group II, late embryogenesis abundant proteins that act putatively as chaperones in stressed plants. To elucidate the function of dehydrins in poplar, we isolated the
-type dehydrin gene Podhn from Populus alba
P. tremula var. glandulosa suspension cells and analyzed its expression following treatments of abiotic stress, wounding and plant growth regulator. Sequence homology and phylogenetic analyses indicate Podhn encodes an acidic dehydrin (pI 5.14, 277 amino acids, predicted size 25.6 kDa) containing two lysine-rich "K-segments" and a 7-serine residue "S-segment", both characteristic of
-type dehydrins. Southern blots show Podhn genes form a small gene family in poplar. Podhn was expressed in all tissues examined under unstressed conditions, but most strongly in cell suspensions (especially in the stationary phase). Drought, salt, cold and exogenous abscisic acid (ABA) treatments enhanced Podhn expression, while wounding and jasmonic acid caused its reduction. Therefore, Podhn might be involved in ABA or stress response.
Cytosolic prion protein induces apoptosis in human neuronal cell SH-SY5Y via mitochondrial disruption pathway
Wang, Xin ; Dong, Chen-Fang ; Shi, Qi ; Shi, Song ; Wang, Gui-Rong ; Lei, Yan-Jun ; Xu, Kun ; An, Run ; Chen, Jian-Ming ; Jiang, Hui-Ying ; Tian, Chan ; Gao, Chen ; Zhao, Yu-Jun ; Han, Jun ; Dong, Xiao-Ping ;
BMB Reports , volume 42, issue 7, 2009, Pages 444~449
DOI : 10.5483/BMBRep.2009.42.7.444
Different neurodegenerative disorders like prion disease, is caused by protein misfolding conformers. Reverse-transfected cytosolic prion protein (PrP) and PrP expressed in the cytosol have been shown to be neurotoxic. To investigate the possible mechanism of neurotoxicity due to accumulation of PrP in cytosol, a PrP mutant lacking the signal and GPI (CytoPrP) was introduced into the SH-SY5Y cell. MTT and trypan blue assays indicated that the viability of cells expressing CytoPrP was remarkably reduced after treatment of MG-132. Obvious apoptosis phenomena were detected in the cells accumulated with CytoPrP, including loss of mitochondrial transmembrane potential, increase of caspase-3 activity, more annexin V/PI-double positive-stained cells and reduced Bcl-2 level. Moreover, DNA fragmentation and TUNEL assays also revealed clear evidences of late apoptosis in the cells accumulated CytoPrP. These data suggest that the accumulation of CytoPrP in cytoplasm may trigger cell apoptosis, in which mitochondrial relative apoptosis pathway seems to play critical role.
Proteome analysis of chloroplast proteins in stage albinism line of winter wheat (triticum aestivum) FA85
Hou, Dian-Yun ; Xu, Hong ; Du, Guang-Yuan ; Lin, Jun-Tang ; Duan, Min ; Guo, Ai-Guang ;
BMB Reports , volume 42, issue 7, 2009, Pages 450~455
DOI : 10.5483/BMBRep.2009.42.7.450
The "stage albinism line of winter wheat" FA85 was a specific natural mutant strain on leaf color. This physiological mutation was controlled by cytogene. In order to reveal the genetic and biochemical mechanism of albinism, 2-DE was used to investigate the difference of chloroplast protein expression pattern between FA85 and its parent wheat Aibian 1. From the results of 2-DE gels analysis, approximately 683 spots were detected on each gel, and 57 spots were expressed differently at least two-fold. Using MALDI-TOF/TOF MS, 14 of 57 spots were identified, which could be categorized into four classes: carbon metabolism, energy metabolism, defense/stress response and signal transduction. Compared with the parent wheat, the expression of ATPase-
was up-regulated in FA85, and of other proteins was down-regulated. Together, we concluded that the expression of chloroplast proteins had changed obviously in FA85, which might be related to the leaf color mutant.
Proteomic characterization of differentially expressed proteins associated with no stress in retinal ganglion cells
Kim, Jum-Ji ; Kim, Yeon-Hyang ; Lee, Mi-Young ;
BMB Reports , volume 42, issue 7, 2009, Pages 456~461
DOI : 10.5483/BMBRep.2009.42.7.456
Proteomic analyses of differentially expressed proteins in rat retinal ganglion cells (RGC-5) following S-nitrosoglutathione (GSNO), an NO donor, treatment were conducted. Of the approximately 314 protein spots that were detected, 19 were differentially expressed in response to treatment with GSNO. Of these, 14 proteins were up-regulated and 5 were down- regulated. Notably, an increase in GAPDH expression following GSNO treatment was detected in RGC-5 cells through Western blotting as well as proteomics. The increased GAPDH expression in response to GSNO treatment was accompanied by an increase in Herc6 protein, an E3 ubiquitin ligase. Moreover, GSNO treatment resulted in the translocation of GADPH from the cytosol to the nucleus and its subsequent accumulation. These results suggest that NO stress-induced apoptosis may be associated with the nuclear translocation and accumulation of GAPDH in RGC-5 cells.
Effects of protein concentration and detergent on endotoxin reduction by ultrafiltration
Jang, Hyun ; Kim, Hyo-Seung ; Moon, Seung-Cheol ; Lee, Young-Rae ; Yu, Kang-Yeoul ; Lee, Byeong-Kil ; Youn, Hyun-Zo ; Jeong, Young-Ju ; Kim, Byeong-Soo ; Lee, Sung-Ho ; Kim, Jong-Suk ;
BMB Reports , volume 42, issue 7, 2009, Pages 462~466
DOI : 10.5483/BMBRep.2009.42.7.462
Lipopolysaccharide (LPS), found in the outer membrane of Gram negative bacteria, only exerts its toxic effects when in free form. LPS has three major parts, lipid A, the toxic component, along with a core polysaccharide and O-specific polysaccharide. LPS monomers are known to have molecular masses between 10 to 30 kDa. Under physiological conditions, LPS exists in equilibrium between monomer and vesicle forms. LPS removal by 100 kDa ultrafiltration was more efficient (99.6% of LPS removed) with a low concentration of protein (2.0 mg/ml) compared to a high concentration (20.1 mg/ml). In the presence of different detergents (0.5% Tween 20, 1.0% taurodeoxycholate and 1.0% Triton X-100), LPS removal was more efficient at low protein concentrations (2.0 mg/ml) compared to high protein concentrations (20.1 mg/ml).