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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal Basic Information
Journal DOI :
Korean Society for Biochemistry and Molecular Biology
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Volume & Issues
Volume 42, Issue 12 - Dec 2009
Volume 42, Issue 11 - Nov 2009
Volume 42, Issue 10 - Oct 2009
Volume 42, Issue 9 - Sep 2009
Volume 42, Issue 8 - Aug 2009
Volume 42, Issue 7 - Jul 2009
Volume 42, Issue 6 - Jun 2009
Volume 42, Issue 5 - May 2009
Volume 42, Issue 4 - Apr 2009
Volume 42, Issue 3 - Mar 2009
Volume 42, Issue 2 - Feb 2009
Volume 42, Issue 1 - Jan 2009
Selecting the target year
Aberrant phosphorylation in the pathogenesis of Alzheimer's disease
Chung, Sul-Hee ;
BMB Reports , volume 42, issue 8, 2009, Pages 467~474
DOI : 10.5483/BMBRep.2009.42.8.467
The modification of proteins by reversible phosphorylation is a key mechanism in the regulation of various physiological functions. Abnormal protein kinase or phosphatase activity can cause disease by altering the phosphorylation of critical proteins in normal cellular and disease processes. Alzheimer' disease (AD), typically occurring in the elderly, is an irreversible, progressive brain disorder characterized by memory loss and cognitive decline. Accumulating evidence suggests that protein kinase and phosphatase activity are altered in the brain tissue of AD patients. Tau is a highly recognized phosphoprotein that undergoes hyperphosphorylation to form neurofibrillary tangles, a neuropathlogical hallmark with amyloid plaques in AD brains. This study is a brief overview of the altered protein phosphorylation pathways found in AD. Understanding the molecular mechanisms by which the activities of protein kinases and phosphatases are altered as well as the phosphorylation events in AD can potentially reveal novel insights into the role aberrant phosphorylation plays in the pathogenesis of AD, providing support for protein phosphorylation as a potential treatment strategy for AD.
Insulin resistance and Alzheimer's disease
De La Monte, Suzanne M. ;
BMB Reports , volume 42, issue 8, 2009, Pages 475~481
DOI : 10.5483/BMBRep.2009.42.8.475
Emerging data demonstrate pivotal roles for brain insulin resistance and insulin deficiency as mediators of cognitive impairment and neurodegeneration, particularly Alzheimer's disease (AD). Insulin and insulin-like growth factors (IGFs) regulate neuronal survival, energy metabolism, and plasticity, which are required for learning and memory. Hence, endogenous brain-specific impairments in insulin and IGF signaling account for the majority of AD-associated abnormalities. However, a second major mechanism of cognitive impairment has been linked to obesity and Type 2 diabetes (T2DM). Human and experimental animal studies revealed that neurodegeneration associated with peripheral insulin resistance is likely effectuated via a liver-brain axis whereby toxic lipids, including ceramides, cross the blood brain barrier and cause brain insulin resistance, oxidative stress, neuro-inflammation, and cell death. In essence, there are dual mechanisms of brain insulin resistance leading to AD-type neurodegeneration: one mediated by endogenous, CNS factors; and the other, peripheral insulin resistance with excess cytotoxic ceramide production.
Oenanthe javanica extract accelerates ethanol metabolism in ethanol-treated animals
Kim, Jong-Yeon ; Kim, Ki-Hoon ; Lee, Youn-Ju ; Lee, Seung-Ho ; Park, Jong-Cheol ; Nam, Doo-Hyun ;
BMB Reports , volume 42, issue 8, 2009, Pages 482~485
DOI : 10.5483/BMBRep.2009.42.8.482
The effect of water dropwort (Oenanthe javanica DC) extract in eliminating ethanol was evaluated in New Zealand white rabbit and ICR mice. When a hot-water extract of water dropwort extract and ethanol was injected into New Zealand white rabbit, the plasma ethanol level was rapidly reduced, similar to metadoxine treatment. Specifically, the n-butanol fraction of hot-water extract was the strongest in eliminating plasma alcohol in ICR mice. When ethanol was orally ingested, administration of the hot-water extract eliminated up to 44% of the plasma ethanol in mice while the n-butanol fraction eliminated around 70%. Alcohol removal behaved in a dose-dependent manner in response to 50-200 mg/kg of n-butanol fraction. These data show O. javanica extract is effective in overcoming alcohol intoxication by the accelerating ethanol metabolism.
Expression of a rice DREB1 gene, OsDREB1D, enhances cold and high-salt tolerance in transgenic Arabidopsis
Zhang, Yang ; Chen, Chen ; Jin, Xiao-Fen ; Xiong, Ai-Sheng ; Peng, Ri-He ; Hong, Yi-Huan ; Yao, Quan-Hong ; Chen, Jian-Min ;
BMB Reports , volume 42, issue 8, 2009, Pages 486~492
DOI : 10.5483/BMBRep.2009.42.8.486
OsDREB1D, a special DREB (dehydration responsive element binding protein) homologous gene, whose transcripts cannot be detected in rice (Oryza sativa L), either with or without stress treatments, was amplified from the rice genome DNA. The yeast one-hybrid assay revealed that OsDREB1D was able to form a complex with the dehydration responsive element/C-repeat motif. It can also bind with a sequence of LTRE (low temperature responsive element). To analyze the function of OsDREB1D, the gene was transformed and over-expressed in Arabidopsis thaliana cv. Columbia. Results indicated that the over-expression of OsDREB1D conferred cold and high-salt tolerance in transgenic plants, and that transgenic plants were also insensitive to ABA (abscisic acid). From these data, we deduced that this OsDREB1D gene functions similarly as other DREB transcription factors. The expression of OsDREB1D in rice may be controlled by a special mechanism for the redundancy of function.
Expression of miR-210 during erythroid differentiation and induction of γ-globin gene expression
Bianchi, Nicoletta ; Zuccato, Cristina ; Lampronti, Ilaria ; Borgatti, Monica ; Gambari, Roberto ;
BMB Reports , volume 42, issue 8, 2009, Pages 493~499
DOI : 10.5483/BMBRep.2009.42.8.493
MicroRNAs (miRs) are a family of small noncoding RNAs that regulate gene expression by targeting mRNAs in a sequence specific manner, inducing translational repression or mRNA degradation. In this paper we have first analyzed by microarray the miR-profile in erythroid precursor cells from one normal and two thalassemic patients expressing different levels of fetal hemoglobin (one of them displaying HPFH phenotype). The microarray data were confirmed by RT-PCR analysis, and allowed us to identify miR-210 as an highly expressed miR in the erythroid precursor cells from the HPFH patient. When RT-PCR was performed on mithramycin-induced K562 cells and erythroid precursor cells, miR-210 was found to be induced in time-dependent and dose-dependent fashion, together with increased expression of the fetal
-globin genes. Altogether, the data suggest that miR-210 might be involved in increased expression of
-globin genes in differentiating erythroid cells.
Anti-oxidative effects of Phellinus linteus and red ginseng extracts on oxidative stress-induced DNA damage
Park, Byung-Jae ; Lim, Yeong-Seok ; Lee, Hee-Jung ; Eum, Won-Sik ; Park, Jin-Seu ; Han, Kyu-Hyung ; Choi, Soo-Young ; Lee, Kil-Soo ;
BMB Reports , volume 42, issue 8, 2009, Pages 500~505
DOI : 10.5483/BMBRep.2009.42.8.500
Anti-oxidative effect of Phellinus linteus (P. linteus) and red ginseng extracts on DNA damage induced by reactive oxygen species (ROS) were investigated in this study. P. linteus (PLE) and red ginseng extracts (RGE) inhibited the breaking of E. coli ColE1 plasmid DNA strands as well as nuclear DNA of rat hepatocytes damaged by oxidative stress. In addition, a reaction mixture of PLE and RGE showed synergistic inhibitory effect against DNA damage. These results suggest that PLE and RGE have a cellular defensive effect against DNA damage induced by ROS.
N-terminal GNBP homology domain of Gram-negative binding protein 3 functions as a beta-1,3-glucan binding motif in Tenebrio molitor
Lee, Han-Na ; Kwon, Hyun-Mi ; Park, Ji-Won ; Kurokawa, Kenji ; Lee, Bok-Luel ;
BMB Reports , volume 42, issue 8, 2009, Pages 506~510
DOI : 10.5483/BMBRep.2009.42.8.506
The Toll signalling pathway in invertebrates is responsible for defense against Gram-positive bacteria and fungi, leading to the expression of antimicrobial peptides via NF-
B-like transcription factors. Gram-negative binding protein 3 (GNBP3) detects beta-1,3-glucan, a fungal cell wall component, and activates a three step serine protease cascade for activation of the Toll signalling pathway. Here, we showed that the recombinant N-terminal domain of Tenebrio molitor GNBP3 bound to beta-1,3-glucan, but did not activate down-stream serine protease cascade in vitro. Reversely, the N-terminal domain blocked GNBP3-mediated serine protease cascade activation in vitro and also inhibited beta-1,3-glucan-mediated antimicrobial peptide induction in Tenebrio molitor larvae. These results suggest that the N-terminal GNBP homology domain of GNBP3 functions as a beta-1,3-glucan binding domain and the C-terminal domain of GNBP3 may be required for the recruitment of immediate down-stream serine protease zymogen during Toll signalling pathway activation.
DOBI is cleaved by caspases during TRAIL-induced apoptotic cell death
Park, Sun-Young ; Shin, Jin-Na ; Woo, Ha-Na ; Piya, Su-Jan ; Moon, Ae-Ran ; Seo, Young-Woo ; Seol, Dai-Wu ; Kim, Tae-Hyoung ;
BMB Reports , volume 42, issue 8, 2009, Pages 511~515
DOI : 10.5483/BMBRep.2009.42.8.511
Downstream of Bid (DOBI) known as Pus10, has been identified as a modulator of TRAIL-induced cell death using RNAi library screening. The crystal structure of DOBI has revealed that it is a crescent-shaped protein containing the pseudouridine synthase catalytic domain and a THUMP-containing domain. Here, we demonstrated that DOBI is expressed in various tissues such as heart and lung, and is also expressed in various tumor cells such as HeLa and A549. Although ectopic expression of DOBI does not promote TRAIL death signaling in HeLa cells, knock-down of DOBI expression using shRNA inhibited TRAIL death signaling. DOBI is cleaved into a 54 kD cleaved DOBI during cell death, and the recombinant DOBI protein can be directly cleaved by caspases-3, or -8 in vitro. Together, these data suggest that the cleaved DOBI may acquire a new function, possibly by cooperating with tBid in the mitochondrial event of cell death caused by TRAIL.
SH2D4A regulates cell proliferation via the ERα/PLC-γ/PKC pathway
Li, Tingting ; Li, Wei ; Lu, Jingyu ; Liu, Hong ; Li, Yinghui ; Zhao, Yanyan ;
BMB Reports , volume 42, issue 8, 2009, Pages 516~522
DOI : 10.5483/BMBRep.2009.42.8.516
SH2D4A, comprising a single SH2 domain, is a novel protein of the SH2 signaling protein family. We have previously demonstrated SH2D4A is expressed ubiquitously in various tissues and is located in the cytoplasm. In this study we investigated the function of SH2D4A in human embryonic kidney (HEK) 293 cells using interaction analysis, cell proliferation assays, and kinase activity detection. SH2D4A was found to directly bind to estrogen receptor
), and prevent the recruitment of phospholipase C-
) to ER
. Moreover, we observed its inhibitory effects on estrogen-induced cell proliferation, involving the protein kinase C (PKC) signaling pathway. Together, these findings suggested that SH2D4A inhibited cell proliferation by suppression of the ER
/PKC signaling pathway. SH2D4A may be useful for the development of a new anti-cancer drug acting as an ER signaling modulator.
Cloning and characterization of phosphomannose isomerase from sphingomonas chungbukensis DJ77
Tran, Sinh Thi ; Le, Dung Tien ; Kim, Young-Chang ; Shin, Malshik ; Choi, Jung-Do ;
BMB Reports , volume 42, issue 8, 2009, Pages 523~528
DOI : 10.5483/BMBRep.2009.42.8.523
Phosphomannose isomerase (PMI) catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate in the extracellular polysaccharide (EPS) synthesis pathway. The gene encoding PMI in Sphingomonas chungbukensis DJ77 was cloned and expressed in E. coli. The pmi gene is 1,410 nucleotides long and the deduced amino acid sequence shares high homology with other bifunctional proteins that possess both PMI and GDP-mannose pyrophosphorylase (GMP) activities. The sequence analysis of PMI revealed two domains with three conserved motifs: a GMP domain at the N-terminus and a PMI domain at the C-terminus. Enzyme assays using the PMI protein confirmed its bifunctional activity. Both activities required divalent metal ions such as
. Of these ions,
was found to be the most effective activator of PMI. GDP-D-mannose was found to inhibit the PMI activity, suggesting feedback regulation of this pathway.
ADFP promoter polymorphism associated with marbling score in Korean cattle
Cheong, Hyun-Sub ; Yoon, Du-Hak ; Bae, Joon-Seol ; Kim, Lyoung-Hyo ; Kim, Eun-Mi ; Kim, Ji-On ; Hong, Jin ; Kim, Nae-Soo ; Shin, Hyoung-Doo ;
BMB Reports , volume 42, issue 8, 2009, Pages 529~534
DOI : 10.5483/BMBRep.2009.42.8.529
Marbling score (MS) is the major trait that affects carcass quality in beef cattle. In this study, we investigated the association between genetic polymorphisms of the adipose differentiation-related protein gene (ADFP) and carcass traits in Korean cattle (also known as Hanwoo). Using direct DNA sequencing in 24 unrelated Korean cattle, 25 novel polymorphisms were identified within all exons and their flanking regions of ADFP, including the promoter region (1.5 kb). Among them, 21 polymorphic sites were selected for genotyping in the beef cattle (n = 425). Statistical analyses revealed that one promoter polymorphism (c.-56-18A > G) was associated with MS (P = 0.009). The 'A' allele of c.-56-18A > G exerted a lowering effect on MS, e.g., the lowest MS was found in 'A/A' (MS = 2.09
1.23), intermediate in 'A/G' (MS = 2.11
1.31), and the highest in 'G/G' (MS = 2.47
1.47). Our findings suggest that these polymorphisms in ADFP might be important genetic factors involved in carcass quality in beef cattle.
Kv1.3 voltage-gated K
channel subunit as a potential diagnostic marker and therapeutic target for breast cancer
Jang, Soo-Hwa ; Kang, Kyung-Sun ; Ryu, Pan-Dong ; Lee, So-Yeong ;
BMB Reports , volume 42, issue 8, 2009, Pages 535~539
DOI : 10.5483/BMBRep.2009.42.8.535
(Kv) channels are widely expressed in the plasma membranes of numerous cells such as epithelial cells. Recently, it has been demonstrated that Kv channels are associated with the proliferation of several types of cancer cells. Specifically, Kv1.3 seems to be involved in cancer cell proliferation and apoptosis. In the present study, we examined the expression of Kv1.3 in immortalized and tumorigenic human mammary epithelial cells. We also evaluated the expression level of Kv1.3 in each stage of breast cancer using mRNA isolated from breast cancer patients. In addition, treatment with tetraethylammonium, a Kv channel blocker, suppressed tumorigenic human mammary epithelial cell proliferation. Therefore, Kv1.3 may serve as a novel molecular target for breast cancer therapy while its stage-specific expression pattern may provide a potential diagnostic marker for breast cancer development.