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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society for Biochemistry and Molecular Biology
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Volume & Issues
Volume 43, Issue 12 - Dec 2010
Volume 43, Issue 11 - Nov 2010
Volume 43, Issue 10 - Oct 2010
Volume 43, Issue 9 - Sep 2010
Volume 43, Issue 8 - Aug 2010
Volume 43, Issue 7 - Jul 2010
Volume 43, Issue 6 - Jun 2010
Volume 43, Issue 5 - May 2010
Volume 43, Issue 4 - Apr 2010
Volume 43, Issue 3 - Mar 2010
Volume 43, Issue 2 - Feb 2010
Volume 43, Issue 1 - Jan 2010
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Comparison of structure, function and regulation of plant cold shock domain proteins to bacterial and animal cold shock domain proteins
Chaikam, Vijay ; Karlson, Dale T. ;
BMB Reports , volume 43, issue 1, 2010, Pages 1~8
DOI : 10.5483/BMBRep.2010.43.1.001
The cold shock domain (CSD) is among the most ancient and well conserved nucleic acid binding domains from bacteria to higher animals and plants. The CSD facilitates binding to RNA, ssDNA and dsDNA and most functions attributed to cold shock domain proteins are mediated by this nucleic acid binding activity. In prokaryotes, cold shock domain proteins only contain a single CSD and are termed cold shock proteins (Csps). In animal model systems, various auxiliary domains are present in addition to the CSD and are commonly named Y-box proteins. Similar to animal CSPs, plant CSPs contain auxiliary C-terminal domains in addition to their N-terminal CSD. Cold shock domain proteins have been shown to play important roles in development and stress adaptation in wide variety of organisms. In this review, the structure, function and regulation of plant CSPs are compared and contrasted to the characteristics of bacterial and animal CSPs.
Differential expression of rice calmodulin promoters in response to stimuli and developmental tissue in transgenic tobacco plants
Kim, Yu-Jung ; Cho, Eun-Kyung ; Lee, Soo-In ; Lim, Chae-Oh ; Choi, Young-Ju ;
BMB Reports , volume 43, issue 1, 2010, Pages 9~16
DOI : 10.5483/BMBRep.2010.43.1.009
The promoters of OsCaM1 and OsCaM3 were characterized after sequencing and fused to the reporter gene, GUS. The constructs were then transformed into the tobacco plant. Histochemical analysis of GUS showed different expression patterns in pOsCaM1::GUS and pOsCaM3:: GUS transgenic plants. The expression of pOsCaM1::GUS in 4- to 15-day-old seedlings in particular was observed only in the root, while the expression of pOsCaM3::GUS was detected in both the cotyledons and root. Also, pRCaM1::GUS was detected in all the tissues surrounding the root system, while the presence of pOsCaM3::GUS was observed in the root, except in the root meristem. However, in mature transgenic plants, the expression of pOsCaM1::GUS and OsRCaM3::GUS was scarcely detected. Under wounding stress, the GUS activity of pOsCaM1 and pOsCaM3 was strongly induced, and the activity of pOsCaM3 especially, was retained for long periods. In the phloem, pOsCaM3 activity induced by hormone treatments and abiotic stresses was also identified.
KBTBD7, a novel human BTB-kelch protein, activates transcriptional activities of SRE and AP-1
Hu, Junjian ; Yuan, Wuzhou ; Tang, Ming ; Wang, Yuequn ; Fan, Xiongwei ; Mo, Xiaoyang ; Li, Yongqing ; Ying, Zaochu ; Wan, Yongqi ; Ocorr, Karen ; Bodmer, Rolf ; Deng, Yun ; Wu, Xiushan ;
BMB Reports , volume 43, issue 1, 2010, Pages 17~22
DOI : 10.5483/BMBRep.2010.43.1.017
In this study, a novel member of BTB-kelch proteins, named KBTBD7, was cloned from a human embryonic heart cDNA library. The cDNA of KBTBD7 is 3,008 bp long and encodes a protein product of 684 amino acids (77.2 kD). This protein is highly conserved in evolution across different species. Western blot analysis indicates that a 77 kD protein specific for KBTBD7 is wildly expressed in all embryonic tissues examined. In COS-7 cells, KBTBD7 proteins are localized to the cytoplasm. KBTBD7 is a transcription activator when fused to GAL4 DNA-binding domain. Deletion analysis indicates that the BTB domain and kelch repeat motif are main regions for transcriptional activation. Overexpression of KBTBD7 in MCF-7 cells activates the transcriptional activities of activator protein-1 (AP-1) and serum response element (SRE), which can be relieved by siRNA. These results suggest that KBTBD7 proteins may act as a new transcriptional activator in mitogen-activated protein kinase (MAPK) signaling.
Role of cysteine at positions 67, 161 and 241 of a Bacillus sphaericus binary toxin BinB
Boonyos, Patcharaporn ; Soonsanga, Sumarin ; Boonserm, Panadda ; Promdonkoy, Boonhiang ;
BMB Reports , volume 43, issue 1, 2010, Pages 23~28
DOI : 10.5483/BMBRep.2010.43.1.023
Binary toxin consisting of BinA and BinB from Bacillus sphaericus is toxic to mosquito larvae. BinB is responsible for specific binding to the larval gut cell membrane while BinA is crucial for toxicity. To investigate functional role of cysteine in BinB, three cysteine residues at positions 67, 161, and 241 were replaced by alanine or serine. Mutations at these positions did not affect protein production and overall structure of BinB. These cysteine residues are not involved in disulfide bond formation between BinB molecules. Mosquito-larvicidal assays revealed that C67 and C161 are essential for toxicity, whereas C241 is not. Mutations at C67 and C161 resulted in weaker BinA-BinB interaction. The loss of toxicity may be due to the reduction of interactions between BinA and BinB or BinB and its receptor. C67 and C161 could also play a part during conformational changes or internalization of the binary toxin into the target cell.
Expression patterns of PRDM10 during mouse embryonic development
Park, Jin-Ah ; Kim, Keun-Cheol ;
BMB Reports , volume 43, issue 1, 2010, Pages 29~33
DOI : 10.5483/BMBRep.2010.43.1.029
It is well known that PR/SET family members participate in transcriptional regulation via chromatin remodeling. PRDM10 might play an essential role in gene expression, but no such evidence has been observed so far. To assess PRDM10 expression at various stages of mouse development, we performed immunohistochemistry using available PRDM10 antibody. Embryos were obtained from three distinct developmental stages. At E8.5, PRDM10 expression was concentrated in the mesodermal and neural crest populations. As embryogenesis proceeded further to E13.5, PRMD10 expression was mainly in mesoderm-derived tissues such as somites and neural crest-derived populations such as the facial skeleton. This expression pattern was consistently maintained to the fetal growth period E16.5 and adult mouse, suggesting that PRDM10 may function in tissue differentiation. Our study revealed that PRDM10 might be a transcriptional regulator for normal tissue differentiation during mouse embryonic development.
OsAREB1, an ABRE-binding protein responding to ABA and glucose, has multiple functions in Arabidopsis
Jin, Xiao-Fen ; Xiong, Ai-Sheng ; Peng, Ri-He ; Liu, Jin-Ge ; Gao, Feng ; Chen, Jian-Min ; Yao, Quan-Hong ;
BMB Reports , volume 43, issue 1, 2010, Pages 34~39
DOI : 10.5483/BMBRep.2010.43.1.034
Expression patterns of OsAREB1 revealed that expression of OsAREB1 gene can be induced by ABA, PEG and heat. Yeast one-hybrid assay demonstrated it can bind to ABA-responsive element (ABRE), which was found in most stress-induced genes. Transgenic Arabidopsis over-expressing OsAREB1 had different responses to ABA and glucose compared to wild-type plants, which suggest OsAREB1 might have a crucial role in these two signaling pathways. Further analysis indicate that OsAREB1 have multiple functions in Arabidopsis. First, OsAREB1 transgenic plants had higher resistance to drought and heat, and OsAREB1 up-regulated the ABA/stress related gene such as RD29A and RD29B. Second, it delayed plant flowering time by down-regulating the expression of flowering-related genes, such as FT, SOC1, LFY and AP1. Due to the dates, OsAREB1 may function as a positive regulator in drought/heat stresses response, but a negative regulator in flowering time in Arabidopsis.
Transduced PEP-1-AMPK inhibits the LPS-induced expression of COX-2 and iNOS in Raw264.7 cells
Shin, Min-Jea ; Lee, Yeom-Pyo ; Kim, Dae-Won ; An, Jae-Jin ; Jang, Sang-Ho ; Cho, Sung-Min ; Sheen, Seung-Hoon ; Lee, Hae-Ran ; Kweon, Hae-Yong ; Kang, Seok-Woo ; Lee, Kwang-Gill ; Park, Jin-Seu ; Eum, Won-Sik ; Cho, Yong-Jun ; Choi, Soo-Young ;
BMB Reports , volume 43, issue 1, 2010, Pages 40~45
DOI : 10.5483/BMBRep.2010.43.1.040
AMP-activated protein kinase (AMPK) is a heterotrimeric enzyme that plays a central role in cellular metabolic stress. Modulation of nitric oxide (NO) and cyclooxygenase-2 (COX-2) is considered a promising approach for the treatment of inflammation and neuronal diseases. In this study, the AMPK gene was fused in-frame with PEP-1 peptide in a bacterial expression vector to produce a PEP-1-AMPK fusion protein. Expressed and purified PEP-1-AMPK fusion proteins were transduced efficiently into macrophage Raw 264.7 cells in a time- and dose-dependent manner. Furthermore, transduced PEP-1-AMPK fusion protein markedly inhibited LPS-induced iNOS and COX-2 expression. These results suggest that the PEP-1-AMPK fusion protein can be used for the protein therapy of COX-2 and NO-related disorders such as inflammation and neuronal diseases.
Protection of burn-induced skin injuries by the flavonoid kaempferol
Park, Byoung-Kwon ; Lee, Soo-Hyoung ; Seo, Jae-Nam ; Rhee, Jae-Won ; Park, Jae-Bong ; Kim, Yong-Sun ; Choi, Ihn-Geun ; Kim, Young-Eun ; Lee, Young-Hee ; Kwon, Hyung-Joo ;
BMB Reports , volume 43, issue 1, 2010, Pages 46~51
DOI : 10.5483/BMBRep.2010.43.1.046
Thermal burn injury induces inflammatory cell infiltrates in the dermis and thickening of the epidermis. Following a burn injury, various mediators, including reactive oxygen species (ROS), are produced in macrophages and neutrophils, exposing all tissues to oxidative injury. The anti-oxidant activities of flavonoids have been widely exploited to scavenge ROS. In this study, we observed that several flavonoids-kaempferol, quercetin, fisetin, and chrysin-inhibit LPS-induced IL-8 promoter activation in RAW 264.7 cells. In contrast with quercetin and fisetin, pretreatment of kaempferol and chrysin did not decrease cell viability. Inflammatory cell infiltrates in the dermis and thickening of the epidermis induced by burn injuries in mice was relieved by kaempferol treatment. However, the injury was worsened by fisetin, quercetin, and chrysin. Expression of TNF-a induced by burn injuries was decreased by kaempferol. These findings suggest the potential use of kaempferol as a therapeutic in thermal burn-induced skin injuries.
Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells
Kim, Jwa-Young ; Choi, Je-Yong ; Jeong, Jae-Hwan ; Jang, Eun-Sik ; Kim, An-Sook ; Kim, Seong-Gon ; Kwon, Hae-Yong ; Jo, You-Young ; Yeo, Joo-Hong ;
BMB Reports , volume 43, issue 1, 2010, Pages 52~56
DOI : 10.5483/BMBRep.2010.43.1.052
Silk fibroin, produced by the silkworm Bombyx mori, has been widely studied as a scaffold in tissue engineering. Although it has been shown to be slowly biodegradable, cellular responses to degraded silk fibroin fragments are largely unknown. In this study, silk fibroin was added to MG-63 cell cultures, and changes in gene expression in the MG-63 cells were screened by DNA microarray analysis. Genes showing a significant (2-fold) change were selected and their expression changes confirmed by quantitative RT-PCR and western blotting. DNA microarray results showed that alkaline phosphatase (ALP), collagen type-I alpha-1, fibronectin, and transforming growth factor-
expressions significantly increased. The effect of degraded silk fibroin on osteoblastogenic gene expression was confirmed by observing up-regulation of ALP activity in MG-63 cells. The finding that small fragments of silk fibroin are able to increase the expression of osteoblastogenic genes suggests that controlled degradation of silk fibroin might accelerate new bone formation.
Over-expression of JunB inhibits mitochondrial stress and cytotoxicity in human lymphoma cells exposed to chronic oxidative stress
Son, Young-Ok ; Heo, Jung-Sun ; Kim, Tae-Geum ; Jeon, Young-Mi ; Kim, Jong-Ghee ; Lee, Jeong-Chae ;
BMB Reports , volume 43, issue 1, 2010, Pages 57~61
DOI : 10.5483/BMBRep.2010.43.1.057
Activator protein-1 can induce either cell survival or death, which is controlled by opposing effects of different Jun members. It is generally accepted that c-Jun is pro-apoptotic, but that JunD is anti-apoptotic in stress-exposed cells. Additionally, although there are reports suggesting that JunB plays a protective role, its role in stress-induced apoptosis remains unclear. Here, we investigated the role of JunB in
-induced cell death using cells that over-expressed the protein or were transfected with si-JunB. Inhibition of JunB expression accelerated
-mediated loss of mitochondrial membrane potential (MMP) and cytotoxicity. Conversely, over-expression of JunB protein led to significant inhibition of the MMP loss and cell death. The increase in JunB expression also attenuated nuclear relocation of apoptosis-inducing factor and mitochondrial Bcl-2 reduction that occurred following
exposure. These results suggest that JunB can signal survival against oxidant-mediated cell death by suppressing mitochondrial stress.
Inhibition of the expression on MMP-2, 9 and morphological changes via human fibrosarcoma cell line by 6,6'-bieckol from marine alga Ecklonia cava
Zhang, Chen ; Li, Yong ; Shi, Xiujuan ; Kim, Se-Kwon ;
BMB Reports , volume 43, issue 1, 2010, Pages 62~68
DOI : 10.5483/BMBRep.2010.43.1.062
Matrix Metalloproteinases (MMPs) are a family of zinc-endopeptidases which can degrade extracellular matrix (ECM) components and play important roles in a variety of biological and pathological processes. 6,6'-bieckol isolated and characterized from an edible marine brown alga Ecklonia cava (EC), according to the comprehensive spectral analysis of MS and NMR data. Here the influence of 6,6'-bieckol on expressions of MMPs was examined by zymography and western blot analysis via human fibrosarcoma cell line (HT1080). It is shown that 6,6'-bieckol significantly down regulated the expressions of MMP-2 and -9 in dose-dependent manner. The influence of 6,6'-bieckol on the cell viability and cell behavior of HT1080 cells were also investigated, our dates shown that it suppressed the migration and 3D culture in HT1080 cells. Meanwhile, we explored several signal pathways which may contribute to this process, and found the suppressing of MMPs expressions in HT1080 cells might be due to the suppression of NF-