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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biochemistry and Molecular Biology
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Volume & Issues
Volume 43, Issue 12 - Dec 2010
Volume 43, Issue 11 - Nov 2010
Volume 43, Issue 10 - Oct 2010
Volume 43, Issue 9 - Sep 2010
Volume 43, Issue 8 - Aug 2010
Volume 43, Issue 7 - Jul 2010
Volume 43, Issue 6 - Jun 2010
Volume 43, Issue 5 - May 2010
Volume 43, Issue 4 - Apr 2010
Volume 43, Issue 3 - Mar 2010
Volume 43, Issue 2 - Feb 2010
Volume 43, Issue 1 - Jan 2010
Selecting the target year
A non-replicating oncolytic vector as a novel therapeutic tool against cancer
Kaneda, Yasufumi ;
BMB Reports , volume 43, issue 12, 2010, Pages 773~780
DOI : 10.5483/BMBRep.2010.43.12.773
Cancers are still difficult targets despite recent advances in cancer therapy. Due to the heterogeneity of cancer, a single-treatment modality is insufficient for the complete elimination of cancer cells. Therapeutic strategies from various aspects are needed. Gene therapy has been expected to bring a breakthrough to cancer therapy, but it has not yet been successful. Gene therapy also should be combined with other treatments to enhance multiple therapeutic pathways. In this view, gene delivery vector itself should be equipped with intrinsic anti-cancer activities. HVJ (hemagglutinating virus of Japan; Sendai virus) envelope vector (HVJ-E) was developed to deliver therapeutic molecules. HVJ-E itself possessed anti-tumor activities such as the generation of anti-tumor immunities and the induction of cancer-selective apoptosis. In addition to the intrinsic anti-tumor activities, therapeutic molecules incorporated into HVJ-E enabled to achieve multi-modal therapeutic strategies in cancer treatment. Tumor-targeting HVJ-E was also developed. Thus, HVJ-E will be a novel promising tool for cancer treatment.
Current advances in adenovirus nanocomplexes: more specificity and less immunogenicity
Kang, Eun-Ah ; Yun, Chae-Ok ;
BMB Reports , volume 43, issue 12, 2010, Pages 781~788
DOI : 10.5483/BMBRep.2010.43.12.781
An often overlooked issue in the field of adenovirus (Ad)-mediated cancer gene therapy is its limited capacity for effective systemic delivery. Although primary tumors can be treated effectively with intralesional injection of conventional Ad vectors, systemic metastasis is difficult to cure. Systemic administration of conventional naked Ads leads to acute accumulation of Ad particles in the liver, induction of neutralizing antibody, short blood circulation half-life, non-specific biodistribution in undesired organs, and low selective accumulation in the target disease site. Versatile strategies involving the modification of viral surfaces with polymers and nanomaterials have been designed for the purpose of maximizing Ad anti-tumor activity and specificity by systemic administration. Integration of viral and non-viral nanomaterials will substantially advance both fields, creating new concepts in gene therapeutics. This review focuses on current advances in the development of smart Ad hybrid nanocomplexes based on various design-based strategies for optimal Ad systemic administration.
Cloning and characterization of a novel gene with alternative splicing in murine mesenchymal stem cell line C3H/10T1/2 by gene trap screening
Wang, Mingke ; Sun, Huiqin ; Jiang, Fan ; Han, Jing ; Ye, Feng ; Wang, Tao ; Su, Yongping ; Zou, Zhongmin ;
BMB Reports , volume 43, issue 12, 2010, Pages 789~794
DOI : 10.5483/BMBRep.2010.43.12.789
A novel gene, designated mgt-6, containing four splicing variants, was isolated from a gene trap clone library of C3H/10T1/2 cells transfected with retroviral promoterless gene-trap vector, ROSAFARY. The transcript variants were differentially expressed in murine tissues and cell lines and differentially responded to diverse stimuli including TGF-
and mitogen-activated protein kinase (MAPK) inhibitors. The mgt-6 gene encoded a protein of 37 or 11 amino acid residuals with cytoplasmic distribution. However, when C3H/10T1/2 cells were treated with 5-azacytidine, the protein translocated into cell nucleus as indicated by fused LacZ or C-terminally tagged EGFP. Our preliminary results suggest that further study on the role of mgt-6 gene in cell transformation and differentiation may be of significance.
Huntingtin-interacting protein 1-related is required for accurate congression and segregation of chromosomes
Park, Sun-Joo ;
BMB Reports , volume 43, issue 12, 2010, Pages 795~800
DOI : 10.5483/BMBRep.2010.43.12.795
Huntingtin-interacting protein 1-related (HIP1r) is known to function in clathrin-mediated endocytosis and regulation of the actin cytoskeleton, which occurs continuously in non-dividing cells. This study reports a new function for HIP1r in mitosis. Green fluorescent protein-fused HIP1r localizes to the mitotic spindles. Depletion of HIP1r by RNA interference induces misalignment of chromosomes and prolonged mitosis, which is associated with decreased proliferation of HIP1r-deficeint cells. Chromosome misalignment leads to missegregation and ultimately production of multinucleated cells. Depletion of HIP1r causes persistent activation of the spindle checkpoint in misaligned chromosomes. These findings suggest that HIP1r plays an important role in regulating the attachment of spindle microtubules to chromosomes during mitosis, an event that is required for accurate congression and segregation of chromosomes. This finding may provide new insights that improve the understanding of various human diseases involving HIP1r as well as its fusion genes.
Soluble expression, purification and the role of C-terminal glycine residues in scorpion toxin BmK AGP-SYPU2
Zhang, Rong ; Cui, Yong ; Zhang, Xi ; Yang, Zhuo ; Zhao, Yongshan ; Song, Yong-Bo ; Wu, Chunfu ; Zhang, Jinghai ;
BMB Reports , volume 43, issue 12, 2010, Pages 801~806
DOI : 10.5483/BMBRep.2010.43.12.801
The existence of glycine residues in long-chain scorpion toxins has been well documented. However, their role as analgesics has not been evaluated. To address this issue, we investigated the functional role of glycines in the C-terminal end of Chinese-scorpion toxin from Buthus martensii Karsch (BmK AGP-SYPU2) using site-directed mutagenesis and analgesic activity assays. Recombinant BmK AGP-SYPU2 and its mutants were efficiently expressed in E. coli and purified to homogeneity using immobilized metal ion affinity chromatography (IMAC) and cation exchange chromatography. The mouse-twisting test was used to detect the analgesic activity of BmK AGP-SYPU2 and its mutants. As a result, we identified glycines at the C-terminal end that, when altered, significantly affected analgesic activity. Also, Mut6566 was significantly decreased compared to BmK AGP-SYPU2. These data indicate that the glycines at the C-terminal end are important for the analgesic activity of BmK AGP-SYPU2.
ZAS3 represses NFκB-dependent transcription by direct competition for DNA binding
Hong, Joung-Woo ; Wu, Lai-Chu ;
BMB Reports , volume 43, issue 12, 2010, Pages 807~812
DOI : 10.5483/BMBRep.2010.43.12.807
and ZAS3 are transcription factors that control important cellular processes including immunity, cell survival and apoptosis. Although both proteins bind the
-motif, they produce opposite physiological consequences;
activates transcription, promotes cell growth and is often found to be constitutively expressed in cancer cells, while ZAS3 generally represses transcription, inhibits cell proliferation and is downregulated in some cancers. Here, we show that ZAS3 inhibits
-dependent transcription by competing with
-motif. Transient transfection studies show that N-terminal 645 amino acids is sufficient to repress transcription activated by
, and that the identical region also possesses intrinsic repression activity to inhibit basal transcription from a promoter. Finally, in vitro DNA-protein interaction analysis shows that ZAS3 is able to displace
by competing with
-motif. It is conceivable that ZAS3 has therapeutic potential for controlling aberrant activation of
in various diseases.
Fluridone affects quiescent centre division in the Arabidopsis thaliana root stem cell niche
Han, Woong ; Zhang, Hanma ; Wang, Myeong-Hyeon ;
BMB Reports , volume 43, issue 12, 2010, Pages 813~817
DOI : 10.5483/BMBRep.2010.43.12.813
Plants undergo cell division throughout their life in order to maintain their growth. It is well known that root and shoot tip of plants possess meristems, which contain quiescent cells. Fluridone (1-methyl-3-phenyl-5-(3-trifluromethyl (phenyl))-4-(1H)-pyridinone) is an established inhibitor of both ABA and carotenoid biosynthesis. However, the other functions of fluridone remain undiscovered. In this report, we provide experimental evidence that fluridone plays a role in the division of the quiescent centre of the Arabidopsis root meristem. This study examined the effects of exogenous fluridone and ABA on the development of the stem cell niche in Arabidopsis root. We show that fluridone promoted the division of stem cells in the quiescent centre, whereas exogenous ABA suppressed quiescent centre division. Furthermore, we established a novel regulatory function for fluridone by demonstrating that it plays an important role in postembryonic development.
Rapid functional screening of effective siRNAs against Plk1 and its growth inhibitory effects in laryngeal carcinoma cells
Lan, Huan ; Zhu, Jiang ; Ai, Qing ; Yang, Zhengmei ; Ji, Ying ; Hong, Suling ; Song, Fangzhou ; Bu, Youquan ;
BMB Reports , volume 43, issue 12, 2010, Pages 818~823
DOI : 10.5483/BMBRep.2010.43.12.818
Plk 1 is overexpressed in many human malignancies including laryngeal carcinoma. However, its therapeutic potential has been never examined in laryngeal carcinoma. In the present study, a simple cellular morphology-based strategy was firstly proposed for rapidly screening the effective siRNAs against Plk1. Furthermore, we investigated the effects of Plk1 depletion via a novel identified effective siRNA against Plk1, Plk1 siRNA-607, on human laryngeal carcinoma Hep-2 cells. The results indicated that Plk1 siRNA-607 transfection resulted in a significant inhibition in Plk1 expression in cells, and subsequently caused a dramatic mitotic cell cycle arrest followed by massive apoptotic cell death, and eventually resulted in a significant decrease in growth and viability of the laryngeal carcinoma cells. Taken together, our present study not only suggests a simple strategy for rapidly screening effective siRNAs against Plk1 but also implicates that Plk1 may serve as a potential therapeutic target in human laryngeal carcinoma.
Beta-carboline alkaloids harmaline and harmalol induce melanogenesis through p38 mitogen-activated protein kinase in B16F10 mouse melanoma cells
Park, Sun-Young ; Kim, Young-Hun ; Kim, Young-Hee ; Park, Geun-Tae ; Lee, Sang-Joon ;
BMB Reports , volume 43, issue 12, 2010, Pages 824~829
DOI : 10.5483/BMBRep.2010.43.12.824
Melanin synthesis is regulated by melanocyte specific enzymes and related transcription factors.
-carboline alkaloids including harmaline and harmalol are widely distributed in the environment including several plant families and alcoholic beverages. Presently, melanin content and tyrosinase activity were increased in melanoma cells by harmaline and harmalol in concentration- and time-dependent manners. Increased protein levels of tyrosinase, tyrosinase-related protein-1 (TRP-1), and TRP-2 were also evident. In addition, immunofluorescence and Western blot analyses revealed harmaline and harmalol increased cAMP response element binding protein phosphorylation and microphthalmia-associated transcription factor expression. In addition to studying the signaling that leads to melanogenesis, roles of the p38 MAPK pathways by the harmaline and harmalol were investigated. Harmaline and harmalol induced time-dependent phosphorylation of p38 MAPK. Harmaline and harmalol stimulated melanin synthesis and tyrosinase activity, as well as expression of tyrosinase and TRP-1 and TRP-2 indicating that these harmaline and harmalol induce melanogenesis through p38 MAPK signaling.
Epigenetic modification of retinoic acid-treated human embryonic stem cells
Cheong, Hyun-Sub ; Lee, Han-Chul ; Park, Byung-Lae ; Kim, Hye-Min ; Jang, Mi-Jin ; Han, Yong-Mahn ; Kim, Seun-Young ; Kim, Yong-Sung ; Shin, Hyoung-Doo ;
BMB Reports , volume 43, issue 12, 2010, Pages 830~835
DOI : 10.5483/BMBRep.2010.43.12.830
Epigenetic modification of the genome through DNA methylation is the key to maintaining the differentiated state of human embryonic stem cells (hESCs), and it must be reset during differentiation by retinoic acid (RA) treatment. A genome-wide methylation/gene expression assay was performed in order to identify epigenetic modifications of RA-treated hESCs. Between undifferentiated and RA-treated hESCs, 166 differentially methylated CpG sites and 2,013 differentially expressed genes were discovered. Combined analysis of methylation and expression data revealed that 19 genes (STAP2, VAMP8, C10orf26, WFIKKN1, ELF3, C1QTNF6, C10orf10, MRGPRF, ARSE, LSAMP, CENTD3, LDB2, POU5F1, GSPT2, THY1, ZNF574, MSX1, SCMH1, and RARB) were highly correlated with each other. The results provided in this study will facilitate future investigations into the interplay between DNA methylation and gene expression through further functional and biological studies.
Relationships between genetic polymorphisms and transcriptional profiles for outcome prediction in anticancer agent treatment
Paik, Hyo-Jung ; Lee, Eun-Jung ; Lee, Do-Heon ;
BMB Reports , volume 43, issue 12, 2010, Pages 836~841
DOI : 10.5483/BMBRep.2010.43.12.836
In the era of personal genomics, predicting the individual response to drug-treatment is a challenge of biomedical research. The aim of this study was to validate whether interaction information between genetic and transcriptional signatures are promising features to predict a drug response. Because drug resistance/susceptibilities result from the complex associations of genetic and transcriptional activities, we predicted the inter-relationships between genetic and transcriptional signatures. With this concept, captured genetic polymorphisms and transcriptional profiles were prepared in cancer samples. By splitting ninety-nine samples into a trial set (n