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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biochemistry and Molecular Biology
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Volume & Issues
Volume 43, Issue 12 - Dec 2010
Volume 43, Issue 11 - Nov 2010
Volume 43, Issue 10 - Oct 2010
Volume 43, Issue 9 - Sep 2010
Volume 43, Issue 8 - Aug 2010
Volume 43, Issue 7 - Jul 2010
Volume 43, Issue 6 - Jun 2010
Volume 43, Issue 5 - May 2010
Volume 43, Issue 4 - Apr 2010
Volume 43, Issue 3 - Mar 2010
Volume 43, Issue 2 - Feb 2010
Volume 43, Issue 1 - Jan 2010
Selecting the target year
GP130 cytokines and bone remodelling in health and disease
Sims, Natalie A. ; Walsh, Nicole C. ;
BMB Reports , volume 43, issue 8, 2010, Pages 513~523
DOI : 10.5483/BMBRep.2010.43.8.513
Cytokines that bind to and signal through the gp130 co-receptor subunit include interleukin (IL)-6, IL-11, oncostatin M (OSM), leukemia inhibitory factor (LIF), cardiotrophin-1 (CT-1), and ciliary neutrophic factor (CNTF). Apart from contributing to inflammation, gp130 signalling cytokines also function in the maintenance of bone homeostasis. Expression of each of these cytokines and their ligand-specific receptors is observed in bone and joint cells, and bone-active hormones and inflammatory cytokines regulate their expression. gp130 signalling cytokines have been shown to regulate the differentiation and activity of osteoblasts, osteoclasts and chondrocytes. Furthermore, cytokine and receptor specific gene-knockout mouse models have identified distinct roles for each of these cytokines in regulating bone resorption, bone formation and bone growth. This review will discuss the current models of paracrine and endocrine actions of gp130-signalling cytokines in bone remodelling and growth, as well as their impact in pathologic bone remodelling evident in periodontal disease, rheumatoid arthritis, spondylarthropathies and osteoarthritis.
New understanding of glucocorticoid action in bone cells
Kim, Hyun-Ju ;
BMB Reports , volume 43, issue 8, 2010, Pages 524~529
DOI : 10.5483/BMBRep.2010.43.8.524
Glucocorticoids (GCs) are useful drugs for the treatment of various diseases, but their use for prolonged periods can cause severe side effects such as osteoporosis. GCs have a direct effect on bone cells, where they can arrest bone formation, in part through the inhibition of osteoblast. On the other hand, GCs potently suppress osteoclast resorptive activity by disrupting its cytoskeleton based on the inhibition of RhoA, Rac and Vav3 in response to macrophage colony-stimulating factor. GCs also interfere with microtubule distribution and stability, which are critical for cytoskeletal organization in osteoclasts. Thus, GCs inhibit microtubule-dependent cytoskeletal organization in osteoclasts, which, in the context of bone remodeling, further dampens bone formation.
The effect of CYP1A2 gene polymorphisms on Theophylline metabolism and chronic obstructive pulmonary disease in Turkish patients
Uslu, Ahmet ; Ogus, Candan ; Ozdemir, Tulay ; Bilgen, Turker ; Tosun, Ozgur ; Keser, Ibrahim ;
BMB Reports , volume 43, issue 8, 2010, Pages 530~534
DOI : 10.5483/BMBRep.2010.43.8.530
Cytochrome P450 (CYP) 1A2 gene polymorphisms are thought to be involved in the metabolism of theophylline (TP). We aimed to investigate the effect of CYP1A2*1C, CYP1A2*1D, CYP1A2*1E, and CYP1A2*1F polymorphisms of the CYP1A2 on TP metabolism by PCR-RFLP in 100 Turkish patients with chronic obstructive pulmonary disease (COPD) receiving TP. One hundred and one healthy volunteers were included as control group. The genotype frequencies of the CYP1A2*1D and CYP1A2*1F were found to be significantly different in the patients compared to the controls. The "T" allele at -2467 delT and the "C" allele at -163 C > A in the CYP1A2 displayed association with a significantly increased risk for COPD. "T" allele at -2467 delT was also associated with a high risk of disease severity in COPD. In conclusion, our data suggest that genetic alterations in CYP1A2 may play a role both in the pharmacogenetics of TP and in the development of COPD.
Severely modified lipoprotein properties without a change in cholesteryl ester transfer protein activity in patients with acute renal failure secondary to Hantaan virus infection
Kim, Ji-Hoe ; Park, Hyun-Ho ; Choi, In-Ho ; Kim, Young-Ok ; Cho, Kyung-Hyun ;
BMB Reports , volume 43, issue 8, 2010, Pages 535~540
DOI : 10.5483/BMBRep.2010.43.8.535
Patients with hemorrhagic fever with renal syndrome (HFRS) often exhibit altered serum lipid and lipoprotein profile during the oliguric phase of the disease. Serum lipid and lipoprotein profiles were assessed during the oliguric and recovery phases in six male patients with HFRS. In the oliguric phase of HFRS, the apolipoprotein (apo) C-III content in high-density lipoproteins (HDL) was elevated, whereas the apoA-I content was lowered. The level of expression and activity of antioxidant enzymes were severely reduced during the oliguric phase, while the cholesteryl ester transfer protein activity and protein level were unchanged between the phases. In the oliguric phase, electromobility of
was faster than in the recovery phase. Low-density lipoprotein (LDL) particle size was smaller and the distribution was less homogeneous. Patients with HFRS in the oliguric phase had severely modified lipoproteins in composition and metabolism.
Large scale purification and characterization of recombinant human autotaxin/lysophospholipase D from mammalian cells
Song, Yuanda ; Dilger, Emily ; Bell, Jessica ; Barton, William A. ; Fang, Xianjun ;
BMB Reports , volume 43, issue 8, 2010, Pages 541~546
DOI : 10.5483/BMBRep.2010.43.8.541
We utilized a mammalian expression system to purify and characterize autotaxin (ATX)/lysophospholipase D, an enzyme present in the blood responsible for biosynthesis of lysophosphatidic acid. The human ATX cDNA encoding amino acids 29-915 was cloned downstream of a secretion signal of CD5. At the carboxyl terminus was a thrombin cleavage site followed by the constant domain (Fc) of IgG to facilitate protein purification. The ATX-Fc fusion protein was expressed in HEK293 cells and isolated from conditioned medium of a stable clone by affinity chromatography with Protein A sepharose followed by cleavage with thrombin. The untagged ATX protein was further purified to essential homogeneity by gel filtration chromatography with a yield of approximately 5 mg/liter medium. The purified ATX protein was enzymatically active and biologically functional, offering a useful tool for further biological and structural studies of this important enzyme.
Analysis of copy number variation in 8,842 Korean individuals reveals 39 genes associated with hepatic biomarkers AST and ALT
Kim, Hyo-Young ; Cho, Seo-Ae ; Yu, Jeong-Mi ; Sung, Sam-Sun ; Kim, Hee-Bal ;
BMB Reports , volume 43, issue 8, 2010, Pages 547~553
DOI : 10.5483/BMBRep.2010.43.8.547
Biochemical tests such as aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are useful for diagnosing patients with liver disease. In this study, we tested the association between copy number variation and the hepatic biomarkers AST and ALT based on 8,842 samples from population-based cohorts in Korea. We used Affymetrix Genome-Wide Human 5.0 arrays and identified 10,534 CNVs using HelixTree software. Of the CNVs tested using univariate linear regression, 100 CNVs were significant for AST and 16 were significant for ALT (P < 0.05). We identified 39 genes located within the CNV regions. DKK1 and HS3ST3B1 were shown to play roles in heparan sulfate biosynthesis and the Wnt signaling pathway, respectively. NAF1 and NPY1R were associated with glycoprotein processes and neuropeptide Y receptor activity based on GO categories. PTER, SOX14 and TM7SF4 were expressed in liver. DPYS and CTSC were found to be associated with dihydropyrimidinuria and Papillon-Lefevre syndrome phenotypes using OMIM. NPY5R was found to be associated with dyslipidemia using the Genetic Association Database.
Smad4 mediates malignant behaviors of human ovarian carcinoma cell through the effect on expressions of E-cadherin, plasminogen activator inhibitor-1 and VEGF
Chen, Chen ; Sun, Ming-Zhong ; Liu, Shuqing ; Yeh, Dongmei ; Yu, Lijun ; Song, Yang ; Gong, Linlin ; Hao, Lihong ; Hu, Jun ; Shao, Shujuan ;
BMB Reports , volume 43, issue 8, 2010, Pages 554~560
DOI : 10.5483/BMBRep.2010.43.8.554
Smad4 is involved in cancer progression and metastasis. Using a pair of human syngeneic epithelial ovarian cancer cells with low (HO-8910) and high (HO-8910PM) metastatic abilities, we aimed to reveal the role of Smad4 in ovarian cancer metastasis in vitro. Smad4 was down-regulated in HO-8910PM cell line relative to HO-8910 by implicating Smad4 was probably a potential tumor suppressor gene for ovarian cancer. Re-expression of Smad4 decreased the migration ability and inhibited the invasion capacity of HO-8910PM, while promoted the cell adhesion capacity for HO-8910PM. The stable expression of Smad4 increased the expression of E-cadherin, reduced the expression of plasminogen activator inhibitor-1 (PAI-1) and slightly down-regulated the expression of VEGF. Smad4 suppresses human ovarian cancer cell metastasis potential through its effect on the expressions of PAI-1, E-cadherin and VEGF. Results from current work implicate Smad4 might suppress the invasion and metastasis of human ovarian tumor cells through a TGF-
Enhancement of HIV-1 Tat fusion protein transduction efficiency by bog blueberry anthocyanins
Lee, Sun-Hwa ; Jeong, Hoon-Jae ; Kim, Dae-Won ; Sohn, Eun-Jeong ; Kim, Mi-Jin ; Kim, Duk-Soo ; Kang, Tae-Cheon ; Lim, Soon-Sung ; Kang, Il-Jun ; Cho, Sung-Woo ; Lee, Kil-Soo ; Park, Jin-Seu ; Eum, Won-Sik ; Choi, Soo-Young ;
BMB Reports , volume 43, issue 8, 2010, Pages 561~566
DOI : 10.5483/BMBRep.2010.43.8.561
Though protein transduction domains (PTDs) are well known for the delivery of exogenous therapeutic proteins into living cells, the overall low efficiency of transduction is a serious obstacle. We investigated the effect of bog blueberry anthocyanins (BBA) on protein transduction efficiency and found that BBA enhanced the transduction efficiencies of Tat-SOD fusion protein into HeLa cells and mice skin. The enzymatic activities in the cells and skin tissue in the presence of BBA were markedly increased compared to controls. Further, BBA did not demonstrate any cell toxicity at various concentrations. Although the mechanism is not fully understood, we suggest that BBA might alter the conformation of the membrane, which would indicate that BBA can be used as a protein transduction enhancer for the efficient delivery of therapeutic proteins for a variety of disorders.
Optimizing the binding activity of the AP2/ERF transcription factor with the GCC box element from Brassica napus by directed evolution
Jin, Xiao-Fen ; Zhu, Bo ; Peng, Ri-He ; Jiang, Hai-Hua ; Chen, Jian-Min ; Zhuang, Jing ; Zhang, Jian ; Yao, Quan-Hong ; Xiong, Ai-Sheng ;
BMB Reports , volume 43, issue 8, 2010, Pages 567~572
DOI : 10.5483/BMBRep.2010.43.8.567
In this study, we cloned the ERF-B3 subfamily transcription factor gene BnaERF-B3-hy15 from Brassica napus L. Huyou15. This 600 bp gene encodes a 199 amino acid classic ethylene responsive factor (ERF), which shown no binding or very weak binding GCC box-binding activity by the yeast one-hybrid assay. We used gene shuffling and the yeast one-hybrid system to obtain three mutated sequences that can bind to the GCC box. Sequence analysis indicated that two residues, Gly156 in the AP2 domain and Phe62 at the N-terminal domain were mutated to arginine and serine, respectively. Changes of Gly156 to arginine and Phe62 to serine increased the GCC-binding activity of BnaERF-B3-hy15 and the alter of Gly156 to arginine changed the AP2-domain structure of BnaERF-B3-hy15.
Molecular characterization and inhibition analysis of the acetylcholinesterase gene from the silkworm maggot, Exorista sorbillans
Lang, Guo-Jun ; Zhang, Ming-Yan ; Li, Bao-Ling ; Yu, Lin-Lin ; Lu, Xing-Meng ; Zhang, Chuan-Xi ;
BMB Reports , volume 43, issue 8, 2010, Pages 573~578
DOI : 10.5483/BMBRep.2010.43.8.573
Several organophosphorus (OP) insecticides can selectively kill the silkworm maggot, Exorista sorbillans (Es) (Diptera: Tachinidae), while not obviously affecting the host (Bombyx mori) larvae, but the mechanism is not yet clear. In this study, the cDNA encoding an acetylcholinesterase (AChE) from the field Es was isolated. One point mutation (Gly353Ala) was identified. The Es-353G AChE and Es-353A AChE were expressed in baculovirus-insect cell system, respectively. The inhibition results showed that for eserine and Chlorpyrifos, Es-353A AChE was significantly less sensitive than Es-353G AChE. Meanwhile, comparison of the I50 values of eserine, dichlorvos, Chlorpyrifos and omethoate of recombinant Es AChEs with its host (Bombyx mori) AChEs indicated that, both Es AChEs are more sensitive than B. mori AChEs. The results give an insight of the mechanism that some OP insecticides can selectively kills Es while without distinct effect on its host, B. mori.