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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biochemistry and Molecular Biology
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Volume & Issues
Volume 44, Issue 12 - Dec 2011
Volume 44, Issue 11 - Nov 2011
Volume 44, Issue 10 - Oct 2011
Volume 44, Issue 9 - Sep 2011
Volume 44, Issue 8 - Aug 2011
Volume 44, Issue 7 - Jul 2011
Volume 44, Issue 6 - Jun 2011
Volume 44, Issue 5 - May 2011
Volume 44, Issue 4 - Apr 2011
Volume 44, Issue 3 - Mar 2011
Volume 44, Issue 2 - Feb 2011
Volume 44, Issue 1 - Jan 2011
Selecting the target year
Interrelationship of Runx2 and estrogen pathway in skeletal tissues
Jeong, Jae-Hwan ; Choi, Je-Yong ;
BMB Reports , volume 44, issue 10, 2011, Pages 613~618
DOI : 10.5483/BMBRep.2011.44.10.613
Two key molecules in skeletal tissues are bone formation master transcription factor Runx2 and the steroid hormone estrogen. It is well known that these two molecules play pivotal roles in bone homeostasis; however, the functional interaction between Runx2 and estrogen synthesis in skeletal tissues is largely unknown. Recent studies have indicated that there is a positive relationship between Runx2 and the estrogen biosynthesis pathway. In this review, a possible functional link between Runx2 and estrogen synthesis pathway in skeletal tissues will be discusses as well as the biological significance of this interaction.
BMPs and their clinical potentials
Kim, Mee-Jung ; Choe, Senyon ;
BMB Reports , volume 44, issue 10, 2011, Pages 619~634
DOI : 10.5483/BMBRep.2011.44.10.619
Bone morphogenetic protein (BMP) signaling in diseases is the subject of an overwhelming array of studies. BMPs are excellent targets for treatment of various clinical disorders. Several BMPs have already been shown to be clinically beneficial in the treatment of a variety of conditions, including BMP-2 and BMP-7 that have been approved for clinical application in nonunion bone fractures and spinal fusions. With the use of BMPs increasingly accepted in spinal fusion surgeries, other therapeutic approaches targeting BMP signaling are emerging beyond applications to skeletal disorders. These approaches can further utilize next-generation therapeutic tools such as engineered BMPs and ex vivo-conditioned cell therapies. In this review, we focused to provide insights into such clinical potentials of BMPs in metabolic and vascular diseases, and in cancer.
-regulated ion channels
Cox, Daniel H. ;
BMB Reports , volume 44, issue 10, 2011, Pages 635~646
DOI : 10.5483/BMBRep.2011.44.10.635
Due to its high external and low internal concentration the
ion is used ubiquitously as an intracellular signaling molecule, and a great many
-sensing proteins have evolved to receive and propagate
signals. Among them are ion channel proteins, whose
sensitivity allows internal
to influence the electrical activity of cell membranes and to feedback-inhibit further
entry into the cytoplasm. In this review I will describe what is understood about the
sensing mechanisms of the three best studied classes of
-sensitive ion channels: Large-conductance
channels, and voltage-gated
channels. Great strides in mechanistic understanding have be made for each of these channel types in just the past few years.
Imipramine enhances neuroprotective effect of PEP-1-Catalase against ischemic neuronal damage
Kim, Dae-Won ; Kim, Duk-Soo ; Kim, Mi-Jin ; Kwon, Soon-Won ; Ahn, Eun-Hee ; Jeong, Hoon-Jae ; Sohn, Eun-Jeong ; Dutta, Suman ; Lim, Soon-Sung ; Cho, Sung-Woo ; Lee, Kil-Soo ; Park, Jin-Seu ; Eum, Won-Sik ; Hwang, Hyun-Sook ; Choi, Soo-Young ;
BMB Reports , volume 44, issue 10, 2011, Pages 647~652
DOI : 10.5483/BMBRep.2011.44.10.647
The protein transduction domains have been reported to have potential to deliver the exogenous molecules, including proteins, to living cells. However, poor transduction of proteins limits therapeutic application. In this study, we examined whether imipramine could stimulate the transduction efficiency of PEP-1 fused proteins into astrocytes. PEP-1-catalase (PEP-1-CAT) was transduced into astrocytes in a time- and dose-dependent manner, reducing cellular toxicity induced by
. Additionally, the group of PEP-1-CAT + imipramine showed enhancement of transduction efficiency and therefore increased cellular viability than that of PEP-1-CAT alone. In the gerbil ischemia models, PEP-1-CAT displayed significant neuroprotection in the CA1 region of the hippocampus. Interestingly, PEP-1-CAT + imipramine prevented neuronal cell death and lipid peroxidation more markedly than PEP-1-CAT alone. Therefore, our results suggest that imipramine can be used as a drug to enhance the transduction of PEP-1 fusion proteins to cells or animals and their efficacies against various disorders.
Cloning and molecular characterization of a new fungal xylanase gene from Sclerotinia sclerotiorum S2
Ellouze, Olfa Elleuch ; Loukil, Sana ; Marzouki, Mohamed Nejib ;
BMB Reports , volume 44, issue 10, 2011, Pages 653~658
DOI : 10.5483/BMBRep.2011.44.10.653
Sclerotinia sclerotiorum fungus has three endoxylanases induced by wheat bran. In the first part, a partial xylanase sequence gene (90 bp) was isolated by PCR corresponding to catalytic domains (
strands of this protein). The high homology of this sequence with xylanase of Botryotinia fuckeliana has permitted in the second part to amplify the XYN1 gene. Sequence analysis of DNA and cDNA revealed an ORF of 746 bp interrupted by a 65 bp intron, thus encoding a predicted protein of 226 amino acids. The mature enzyme (20.06 kDa), is coded by 188 amino acid (pI 9.26). XYN1 belongs to G/11 glycosyl hydrolases family with a conserved catalytic domain containing
residues. Bioinformatics analysis revealed that there was no Asn-X-Ser/Thr motif required for N-linked glycosylation in the deduced sequence however, five O-glycosylation sites could intervene in the different folding of xylanses isoforms and in their secretary pathway.
Ginsenoside Rh2(S) induces the differentiation and mineralization of osteoblastic MC3T3-E1 cells through activation of PKD and p38 MAPK pathways
Kim, Do-Yeon ; Jung, Mi-Song ; Park, Young-Guk ; Yuan, Hai Dan ; Quan, Hai Yan ; Chung, Sung-Hyun ;
BMB Reports , volume 44, issue 10, 2011, Pages 659~664
DOI : 10.5483/BMBRep.2011.44.10.659
As part of the search for biologically active anti-osteoporotic agents that enhance differentiation and mineralization of osteoblastic MC3T3-E1 cells, we identified the ginsenoside Rh2(S), which is an active component in ginseng. Rh2(S) stimulates osteoblastic differentiation and mineralization, as manifested by the up-regulation of differentiation markers (alkaline phosphatase and osteogenic genes) and Alizarin Red staining, respectively. Rh2(S) activates p38 mitogen-activated protein kinase (MAPK) in time- and concentration-dependent manners, and Rh2(S)-induced differentiation and mineralization of osteoblastic cells were totally inhibited in the presence of the p38 MAPK inhibitor, SB203580. In addition, pretreatment with Go6976, a protein kinase D (PKD) inhibitor, significantly reversed the Rh2(S)-induced p38 MAPK activation, indicating that PKD might be an upstream kinase for p38 MAPK in MC3T3-E1 cells. Taken together, these results suggest that Rh2(S) induces the differentiation and mineralization of MC3T3-E1 cells through activation of PKD/p38 MAPK signaling pathways, and these findings provide a molecular basis for the osteogenic effect of Rh2(S).
Temperature, organic solvent and pH stabilization of the neutral protease from Salinovibrio proteolyticus: significance of the structural calcium
Asghari, S. Mohsen ; Khajeh, Khosro ; Dalfard, Arastoo Badoei ; Pazhang, Mohammad ; Karbalaei-Heidari, Hamid Reza ;
BMB Reports , volume 44, issue 10, 2011, Pages 665~668
DOI : 10.5483/BMBRep.2011.44.10.665
In order to clarify the impact of Ca-binding sites (Ca1 and 2) on the conformational stability of neutral proteases (NPs), we have analyzed the thermal, pH and organic solvent stability of a NP variant, V189P/A195E/G203D/A268E (Q-mutant), from Salinovibrio proteolyticus. This mutant has shown to bind calcium more tightly than the wild-type (WT) at Ca1 and to possess Ca2. Q-mutant was resisted against autolysis, thermoinactivation and pH denaturation in a Ca-dependent manner and exhibited better activity in organic solvents compared to the WT enzyme. These results imply that Ca1 and Ca2 are important for the conformational stability of NPs.
Identification of an antimicrobial peptide from human methionine sulfoxide reductase B3
Kim, Yong-Joon ; Kwak, Geun-Hee ; Lee, Chu-Hee ; Kim, Hwa-Young ;
BMB Reports , volume 44, issue 10, 2011, Pages 669~673
DOI : 10.5483/BMBRep.2011.44.10.669
Human methionine sulfoxide reductase B3A (hMsrB3A) is an endoplasmic reticulum (ER) reductase that catalyzes the stereospecific reduction of methionine-R-sulfoxide to methionine in proteins. In this work, we identified an antimicrobial peptide from hMsrB3A protein. The N-terminal ER-targeting signal peptide (amino acids 1-31) conferred an antimicrobial effect in Escherichia coli cells. Sequence and structural analyses showed that the overall positively charged ER signal peptide had an Argand Pro-rich region and a potential hydrophobic
-helical segment that contains 4 cysteine residues. The potential
-helical region was essential for the antimicrobial activity within E. coli cells. A synthetic peptide, comprised of 2-26 amino acids of the signal peptide, was effective at killing Gram-negative E. coli, Klebsiella pneumoniae, and Salmonella paratyphi, but had no bactericidal activity against Gram-positive Staphylococcus aureus.
Essential role of tryptophan residues in toxicity of binary toxin from Bacillus sphaericus
Kunthic, Thittaya ; Promdonkoy, Boonhiang ; Srikhirin, Toemsak ; Boonserm, Panadda ;
BMB Reports , volume 44, issue 10, 2011, Pages 674~679
DOI : 10.5483/BMBRep.2011.44.10.674
Bacillus sphaericus produces mosquito-larvicidal binary toxin composed of BinA and BinB. While BinB is expected to bind to a specific receptor on the cell membrane, BinA interacts to BinB or BinB receptor complex and translocates into the cytosol to exert its activity via unknown mechanism. To investigate functional roles of aromatic cluster in BinA, amino acids at positions Y213, Y214, Y215, W222 and W226 were substituted by leucine. All mutant proteins were highly produced and their secondary structures were not affected by these substitutions. All mutants are able to insert into lipid monolayers as observed by Langmuir-Blodgett trough and could permeabilize the liposomes in a similar manner as the wild type. However, mosquito-larvicidal activity was abolished for W222L and W226L mutants suggesting that tryptophan residues at both positions play an important role in the toxicity of BinA, possibly involved in the cytopathological process after toxin entry into the cells.
Comprehensive analysis of AHL homologous genes encoding AT-hook motif nuclear localized protein in rice
Kim, Ho-Bang ; Oh, Chang-Jae ; Park, Yung-Chul ; Lee, Yi ; Choe, Sung-Hwa ; An, Chung-Sun ; Choi, Sang-Bong ;
BMB Reports , volume 44, issue 10, 2011, Pages 680~685
DOI : 10.5483/BMBRep.2011.44.10.680
The AT-hook motif is a small DNA-binding protein motif that has been found in the high mobility group of non-histone chromosomal proteins. The Arabidopsis genome contains 29 genes encoding the AT-hook motif DNA-binding protein (AHL). Recent studies of Arabidopsis genes (AtAHLs) have revealed that they might play diverse functional roles during plant growth and development. In this report, we mined 20 AHL genes (OsAHLs) from the rice genome database using AtAHL genes as queries and characterized their molecular features. A phylogenetic tree revealed that OsAHL proteins can be classified into 2 evolutionary clades. Tissue expression pattern analysis revealed that all of the OsAHL genes might be functionally expressed genes with 3 distinct expression patterns. Nuclear localization analysis using transgenic Arabidopsis showed that several OsAHL proteins are exclusively localized in the nucleus, indicating that they may act as architectural transcription factors to regulate expression of their target genes during plant growth and development.
Enhanced biological effects of Phe140Asn, a novel human granulocyte colony-stimulating factor mutant, on HL60 cells
Chung, Hee-Kyoung ; Kim, Sung-Woo ; Byun, Sung-June ; Ko, Eun-Mi ; Chung, Hak-Jae ; Woo, Jae-Seok ; Yoo, Jae-Gyu ; Lee, Hwi-Cheul ; Yang, Byoung-Chul ; Kwon, Moo-Sik ; Park, Soo-Bong ; Park, Jin-Ki ; Kim, Kyung-Woon ;
BMB Reports , volume 44, issue 10, 2011, Pages 686~691
DOI : 10.5483/BMBRep.2011.44.10.686
Granulocyte colony-stimulating factor (G-CSF) is a cytokine secreted by stromal cells and plays a role in the differentiation of bone marrow stem cells and proliferation of neutrophils. Therefore, G-CSF is widely used to reduce the risk of serious infection in immunocompromised patients; however, its use in such patients is limited because of its non-persistent biological activity. We created an N-linked glycosylated form of this cytokine, hG-CSF (Phe140Asn), to assess its biological activity in the promyelocyte cell line HL60. Enhanced biological effects were identified by analyzing the JAK2/STAT3/survivin pathway in HL60 cells. In addition, mutant hG-CSF (Phe140Asn) was observed to have enhanced chemoattractant effects and improved differentiation efficiency in HL60 cells. These results suggest that the addition of N-linked glycosylation was successful in improving the biological activity of hG-CSF. Furthermore, the mutated product appears to be a feasible therapy for patients with neutropenia.
Isolation and characterization of thioredoxin and NADPH-dependent thioredoxin reductase from tomato (Solanum lycopersicum)
Dai, Changbo ; Wang, Myeong-Hyeon ;
BMB Reports , volume 44, issue 10, 2011, Pages 692~697
DOI : 10.5483/BMBRep.2011.44.10.692
To investigate the pathways of oxidoreductases in plants, 2 key components in thioredox systems i.e. thioredoxin h (Trx h) and NADPH-dependent thioredoxin reductase (NTR) genes were first isolated from tomatoes (Solanum lycopersicum). Subsequently, the coding sequences of Trx h and NTR were inserted into pET expression vectors, and overexpressed in Escherichia coli. In the UV-Visible spectra of the purified proteins, tomato Trx h was shown to have a characteristic 'shoulder' at ~290 nm, while the NTR protein had the 3 typical peaks unique to flavoenzymes. The activities of both proteins were demonstrated by following insulin reduction, as well as DTNB reduction. Moreover, both NADPH and NADH could serve as substrates in the NTR reduction system, but the catalytic efficiency of NTR with NADPH was 2500-fold higher than with NADH. Additionally, our results reveal that the tomato Trx system might be involved in oxidative stress, but not in cold damage.