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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal Basic Information
Journal DOI :
Korean Society for Biochemistry and Molecular Biology
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Volume & Issues
Volume 44, Issue 12 - Dec 2011
Volume 44, Issue 11 - Nov 2011
Volume 44, Issue 10 - Oct 2011
Volume 44, Issue 9 - Sep 2011
Volume 44, Issue 8 - Aug 2011
Volume 44, Issue 7 - Jul 2011
Volume 44, Issue 6 - Jun 2011
Volume 44, Issue 5 - May 2011
Volume 44, Issue 4 - Apr 2011
Volume 44, Issue 3 - Mar 2011
Volume 44, Issue 2 - Feb 2011
Volume 44, Issue 1 - Jan 2011
Selecting the target year
Pros and cons of using aberrant glycosylation as companion biomarkers for therapeutics in cancer
Kang, Jeong-Gu ; Ko, Jeong-Heon ; Kim, Yong-Sam ;
BMB Reports , volume 44, issue 12, 2011, Pages 765~771
DOI : 10.5483/BMBRep.2011.44.12.765
Cancer treatment has been stratified by companion biomarker tests that serve to provide information on the genetic status of cancer patients and to identify patients who can be expected to respond to a given treatment. This stratification guarantees better efficiency and safety during treatment. Cancer patients, however, marginally benefit from the current companion biomarker-aided treatment regimens, presumably because companion biomarker tests are dependent solely on the mutation status of several genes status quo. In the true sense of the term, "personalized medicine", cancer patients are deemed to be identified individually by their molecular signatures, which are not necessarily confined to genetic mutations. Glycosylation is tremendously dynamic and shows alterations in cancer. Evidence is accumulating that aberrant glycosylation contributes to the development and progression of cancer, holding the promise for use of glycosylation status as a companion biomarker in cancer treatment. There are, however, several challenges derived from the lack of a reliable detection system for aberrant glycosylation, and a limited library of aberrant glycosylation. The challenges should be addressed if glycosylation status is to be used as a companion biomarker in cancer treatment and contribute to the fulfillment of personalized medicine.
Branched N-glycans and their implications for cell adhesion, signaling and clinical applications for cancer biomarkers and in therapeutics
Taniguchi, Naoyuki ; Korekane, Hiroaki ;
BMB Reports , volume 44, issue 12, 2011, Pages 772~781
DOI : 10.5483/BMBRep.2011.44.12.772
Branched N-glycans are produced by a series of glycosyltransferases including N-acetylglucosaminyltransferases and fucosyltransferases and their corresponding genes. Glycans on specific glycoproteins, which are attached via the action of glycosyltransferases, play key roles in cell adhesion and signaling. Examples of this are adhesion molecules or signaling molecules such as integrin and E-cadherin, as well as membrane receptors such as the EGF and TGF-
receptors. These molecules also play pivotal roles in the underlying mechanism of a variety of disease such as cancer metastasis, diabetes, and chronic obstructive pulmonary disease (COPD). Alterations in the structures of branched N-glycans are also hall marks and are useful for cancer biomarkers and therapeutics against cancer. This mini-review describes some of our recent studies on a functional glycomics approach to the study of branched N-glycans produced by N-acetylglucosaminyltransferases III, IV, V and IX (Vb) (GnT-III, GnT-IV, V and IX (Vb)) and fucosyltransferase 8 (Fut8) and their pathophysiological significance, with emphasis on the importance of a systems glycobiology approach as a future perspective for glycobiology.
p53 is not necessary for nuclear translocation of GAPDH during NO-induced apoptosis
Kim, Jum-Ji ; Lee, Mi-Young ;
BMB Reports , volume 44, issue 12, 2011, Pages 782~786
DOI : 10.5483/BMBRep.2011.44.12.782
Aberrant GAPDH expression following S-nitrosoglutathione (GSNO) treatment was compared in HepG2 cells, which express functional p53, and Hep3B cells, which lack functional p53. The results of Western blotting and fluorescent immunocytochemistry revealed that nuclear translocation and accumulation of GAPDH occur in both HepG2 and Hep3B cells. This finding suggests that p53 may not be necessary for the GSNO-induced translocation of GAPDH to the nucleus during apoptotic cell death in hepatoma cells.
Effect of silk fibroin peptide derived from silkworm Bombyx mori on the anti-inflammatory effect of Tat-SOD in a mice edema model
Kim, Dae-Won ; Hwang, Hyun-Sook ; Kim, Duk-Soo ; Sheen, Seung-Hoon ; Heo, Dong-Hwa ; Hwang, Gyo-Jun ; Kang, Suk-Hyung ; Kweon, Hae-Yong ; Jo, You-Young ; Kang, Seok-Woo ; Lee, Kwang-Gill ; Park, Kye-Won ; Han, Kyu-Hyung ; Park, Jin-Seu ; Eum, Won-Sik ; Cho, Yong-Jun ; Choi, Hyun-Chul ; Choi, Soo-Young ;
BMB Reports , volume 44, issue 12, 2011, Pages 787~792
DOI : 10.5483/BMBRep.2011.44.12.787
We investigated whether silk fibroin peptide derived from the silkworm, Bombyx mori, could inhibit inflammation and enhance the anti-inflammatory activity of Tat-superoxide dismutase (Tat-SOD), which was previously reported to effectively penetrate various cells and tissues and exert anti-oxidative activity in a mouse model of inflammation. Inflammation was induced by topical treatment of mouse ears with 12-O-tetradecanoylphorbol-13-acetate (TPA). Histological, Western blot, and reverse transcription-polymerase chain reaction data demonstrated that silk fibroin peptide or Tat-SOD alone could suppress elevated levels of cyclooxygenase-2, interleukin-6, interleukin-1beta, and tumor necrosis factor-alpha induced by TPA. Moreover, silk fibroin peptide significantly enhanced the anti-inflammatory activity of Tat-SOD, although it had no influence on in vitro and in vivo transduction of Tat-SOD. Silk fibroin peptide exhibited anti-inflammatory activity in a mice model of inflammation. Therefore, silk fibroin peptide alone or in combination with Tat-SOD might be used as a therapeutic agent for various inflammatory diseases.
Assessment of the effects of virus-mediated limited Oct4 overexpression on the structure of the hippocampus and behavior in mice
Sim, Su-Eon ; Park, Soo-Won ; Choi, Sun-Lim ; Yu, Nam-Kyung ; Ko, Hyoung-Gon ; Jang, Deok-Jin ; Lee, Kyung-Min ; Kaang, Bong-Kiun ;
BMB Reports , volume 44, issue 12, 2011, Pages 793~798
DOI : 10.5483/BMBRep.2011.44.12.793
Recently, pluripotency induction or cellular reprogramming by introducing critical transcription factors has been extensively studied, but has been demonstrated only in vitro. Based on reports that Oct4 is critically involved in transforming neural stem cells into pluripotent cells, we used the lentiviral vector to introduce the Oct4 gene into the hippocampal dentate gyrus (DG) of adult mice. We examined whether this manipulation led to cellular or behavioral changes, possibly through processes involving the transformation of NS cells into pluripotent cells. The Oct4 lentivirus-infused group and the green fluorescent protein lentivirus-infused group showed a similar thickness of the DG and a comparable level of synaptophysin expression in the DG. Furthermore, our behavioral analyses did not show any differences between the groups concerning exploratory activity, anxiety, or memory abilities. This first trial for pluripotency induction in vivo, despite negative results, provides implications and information for future studies on in vivo cellular reprogramming.
Regulatory roles of ganglioside GQ1b in neuronal cell differentiation of mouse embryonic stem cells
Kwak, Dong-Hoon ; Jin, Jung-Woo ; Ryu, Jae-Sung ; Ko, Kinram ; Lee, So-Dam ; Lee, Jeong-Woong ; Kim, Ji-Su ; Jung, Kyu-Yong ; Ko, Ki-Sung ; Ma, Jin-Yeul ; Hwang, Kyung-A ; Chang, Kyu-Tae ; Choo, Young-Kug ;
BMB Reports , volume 44, issue 12, 2011, Pages 799~804
DOI : 10.5483/BMBRep.2011.44.12.799
Gangliosides play an important role in neuronal differentiation processes. The regulation of ganglioside levels is related to the induction of neuronal cell differentiation. In this study, the ST8Sia5 gene was transfected into mESCs and then differentiated into neuronal cells. Interestingly, ST8Sia5 gene transfected mESCs expressed GQ1b by HPTLC and immunofluorescence analysis. To investigate the effects of GQ1b over-expression in neurogenesis, neuronal cells were differentiated from GQ1b expressing mESCs in the presence of retinoic acid. In GQ1b expressing mESCs, increased EBs formation was observed. After 4 days, EBs were co-localized with GQ1b and nestin, and GFAP. Moreover, GQ1b co-localized with MAP-2 expressing cells in GQ1b expressing mESCs in 7-day-old EBs. Furthermore, GQ1b expressing mESCs increased the ERK1/2 MAP kinase pathway. These results suggest that the ST8Sia5 gene increases ganglioside GQ1b and improves neuronal differentiation via the ERK1/2 MAP kinase pathway.
Characterization of a novel methionine sulfoxide reductase A from tomato (Solanum lycopersicum), and its protecting role in Escherichia coli
Dai, Changbo ; Singh, Naresh Kumar ; Park, Myung-Ho ;
BMB Reports , volume 44, issue 12, 2011, Pages 805~810
DOI : 10.5483/BMBRep.2011.44.12.805
Methionine sulfoxide reductase A (MSRA) is a ubiquitous enzyme that has been demonstrated to reduce the S enantiomer of methionine sulfoxide (MetSO) to methionine (Met) and can protect cells against oxidative damage. In this study, we isolated a novel MSRA (SlMSRA2) from Micro-Tom (Solanum lycopersicum L. cv. Micro-Tom) and characterized it by subcloning the coding sequence into a pET expression system. Purified recombinant protein was assayed by HPLC after expression and refolding. This analysis revealed the absolute specificity for methionine-S-sulfoxide and the enzyme was able to convert both free and protein-bound MetSO to Met in the presence of DTT. In addition, the optimal pH, appropriate temperature, and
values for MSRA2 were observed as 8.5,
, respectively. Disk inhibition and growth rate assays indicated that SlMSRA2 may play an essential function in protecting E. coli against oxidative damage.
Identification of a lead small-molecule inhibitor of anthrax lethal toxin by using fluorescence-based high-throughput screening
Wei, Dong ; Bu, Zhaoyun ; Yu, Ailian ; Li, Feng ;
BMB Reports , volume 44, issue 12, 2011, Pages 811~815
DOI : 10.5483/BMBRep.2011.44.12.811
Inhalational anthrax is caused by B. anthracis, a virulent sporeforming bacterium which secretes anthrax toxins consisting of protective antigen (PA), lethal factor (LF) and edema factor (EF). LF is a Zn-dependent metalloprotease and is the main determinant in the pathogenesis of anthrax. Here we report the identification of a lead small-molecule inhibitor of anthrax lethal factor by screening an available synthetic small-molecule inhibitor library using fluorescence-based high-throughput screening (HTS) approach. Seven small molecules were found to have inhibitory effect against LF activity, among which SM157 had the highest inhibitory activity. All theses small molecule inhibitors inhibited LF in a noncompetitive inhibition mode. SM157 and SM167 are from the same family, both having an identical group complex, which is predicted to insert into S1' pocket of LF. More potent small-molecule inhibitors could be developed by modifying SM157 based on this identical group complex.
Tobacco mitochondrial small heat shock protein NtHSP24.6 adopts a dimeric configuration and has a broad range of substrates
Kim, Keun-Pill ; Yu, Ji-Hee ; Park, Soo-Min ; Koo, Hyun-Jo ; Hong, Choo-Bong ;
BMB Reports , volume 44, issue 12, 2011, Pages 816~820
DOI : 10.5483/BMBRep.2011.44.12.816
There is a broad range of different small heat shock proteins (sHSPs) that have diverse structural and functional characteristics. To better understand the functional role of mitochondrial sHSP, NtHSP24.6 was expressed in Escherichia coli with a hexahistidine tag and purified. The protein was analyzed by non-denaturing PAGE, chemical cross-linking and size exclusion chromatography and the
protein was found to form a dimer in solution. The in vitro functional analysis of
using firefly luciferase and citrate synthase demonstrated that this protein displays typical molecular chaperone activity. When cell lysates of E. coli were heated after the addition of
, a broad range of proteins from 10 to 160 kD in size remained in the soluble state. These results suggest that NtHSP24.6 forms a dimer and can function as a molecular chaperone to protect a diverse range of proteins from thermal aggregation.