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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biochemistry and Molecular Biology
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Volume & Issues
Volume 45, Issue 12 - Dec 2012
Volume 45, Issue 11 - Nov 2012
Volume 45, Issue 10 - Oct 2012
Volume 45, Issue 9 - Sep 2012
Volume 45, Issue 8 - Aug 2012
Volume 45, Issue 7 - Jul 2012
Volume 45, Issue 6 - Jun 2012
Volume 45, Issue 5 - May 2012
Volume 45, Issue 4 - Apr 2012
Volume 45, Issue 3 - Mar 2012
Volume 45, Issue 2 - Feb 2012
Volume 45, Issue 1 - Jan 2012
Selecting the target year
Characterization and function of human Ly-6/uPAR molecules
Kong, Hyun Kyung ; Park, Jong Hoon ;
BMB Reports , volume 45, issue 11, 2012, Pages 595~603
DOI : 10.5483/BMBRep.2012.45.11.210
Human Ly-6/uPAR molecules are a superfamily composed of two subfamilies; one is the membrane bound proteins with a GPI-anchor and the other are secreted proteins without the GPI-anchor. Ly-6/uPAR molecules have remarkable amino acid homology through a distinctive 8-10 cysteine-rich domain that is associated predominantly with O-linked glycans. These molecules are encoded by multiple tightly linked genes located on Chr. 8q23, and have a conserved genomic organization. Ly-6/uPAR molecules have an interesting expression pattern during hematopoiesis and on specific tumors indicating that Ly-6/uPAR molecules are associated with development of the immune system and carcinogenesis. Thus, Ly-6/uPAR molecules are useful antigens for diagnostic and therapeutic targets. This review summarizes our understanding of human Ly-6/uPAR molecules with regard to molecular structure as well as what is known about their function in normal and malignant tissues and suggest Ly-6/uPAR molecules as target antigens for cancer immunotherapy.
Long noncoding RNA involvement in cancer
Maruyama, Reo ; Suzuki, Hiromu ;
BMB Reports , volume 45, issue 11, 2012, Pages 604~611
DOI : 10.5483/BMBRep.2012.45.11.227
Recent advances in genome and transcriptome analysis have enabled identification of numerous members of a new class of noncoding RNA, long noncoding RNA (lncRNA). lncRNAs are broadly defined as RNA molecules greater than 200 nt in length and lacking an open reading frame. Recent studies provide evidence that lncRNAs play central roles in a wide range of cellular processes through interaction with key component proteins in the gene regulatory system, and that alteration of their cell- or tissue-specific expression and/or their primary or secondary structures is thought to promote cell proliferation, invasion and metastasis. The biological and molecular characteristics of the large majority of lncRNAs remains unknown, and it is anticipated that improved understanding of the roles played by lncRNAs in cancer will lead to the development of novel biomarkers and effective therapeutic strategies.
Olfactory receptors in non-chemosensory tissues
Kang, NaNa ; Koo, JaeHyung ;
BMB Reports , volume 45, issue 11, 2012, Pages 612~622
DOI : 10.5483/BMBRep.2012.45.11.232
Olfactory receptors (ORs) detect volatile chemicals that lead to the initial perception of smell in the brain. The olfactory receptor (OR) is the first protein that recognizes odorants in the olfactory signal pathway and it is present in over 1,000 genes in mice. It is also the largest member of the G protein-coupled receptors (GPCRs). Most ORs are extensively expressed in the nasal olfactory epithelium where they perform the appropriate physiological functions that fit their location. However, recent whole-genome sequencing shows that ORs have been found outside of the olfactory system, suggesting that ORs may play an important role in the ectopic expression of non-chemosensory tissues. The ectopic expressions of ORs and their physiological functions have attracted more attention recently since MOR23 and testicular hOR17-4 have been found to be involved in skeletal muscle development, regeneration, and human sperm chemotaxis, respectively. When identifying additional expression profiles and functions of ORs in non-olfactory tissues, there are limitations posed by the small number of antibodies available for similar OR genes. This review presents the results of a research series that identifies ectopic expressions and functions of ORs in non-chemosensory tissues to provide insight into future research directions.
Expression level and glycan dynamics determine the net effects of TIMP-1 on cancer progression
Kim, Yong-Sam ; Kim, Sun-Hee ; Kang, Jeong-Gu ; Ko, Jeong-Heon ;
BMB Reports , volume 45, issue 11, 2012, Pages 623~628
DOI : 10.5483/BMBRep.2012.45.11.233
Tissue inhibitor of metalloproteinases (TIMPs; TIMP-1, -2, -3 and -4) are endogenous inhibitor for matrix metalloproteinases (MMPs) that are responsible for remodeling the extracellular matrix (ECM) and involved in migration, invasion and metastasis of tumor cells. Unlike under normal conditions, the imbalance between MMPs and TIMPs is associated with various diseased states. Among TIMPs, TIMP-1, a 184-residue protein, is the only N-linked glycoprotein with glycosylation sites at N30 and N78. The structural analysis of the catalytic domain of human stromelysin-1 (MMP-3) and human TIMP-1 suggests new possibilities of the role of TIMP-1 glycan moieties as a tuner for the proteolytic activities by MMPs. Because the TIMP-1 glycosylation participate in the interaction, aberrant glycosylation of TIMP-1 presumably affects the interaction, thereby leading to pathogenic dysfunction in cancer cells. TIMP-1 has not only the cell proliferation activities but also anti-oncogenic properties. Cancer cells appear to utilize these bilateral aspects of TIMP-1 for cancer progression; an elevated TIMP-1 level exerts to cancer development via MMP-independent pathway during the early phase of tumor formation, whereas it is the aberrant glycosylation of TIMP-1 that overcome the high anti-proteolytic burden. The aberrant glycosylation of TIMP-1 can thus be used as staging and/or prognostic biomarker in colon cancer.
STC2 is upregulated in hepatocellular carcinoma and promotes cell proliferation and migration in vitro
Wang, Haixiao ; Wu, Kuangjie ; Sun, Yuan ; Li, Yandong ; Wu, Mingyu ; Qiao, Qian ; Wei, Yuanjiang ; Han, Ze-Guang ; Cai, Bing ;
BMB Reports , volume 45, issue 11, 2012, Pages 629~634
DOI : 10.5483/BMBRep.2012.45.11.086
The human glycoprotein, stanniocalcin 2 (STC2) plays multiple roles in several tumor types, however, its function and clinical significance in hepatocellular carcinoma (HCC) remain unclear. In this study, we detected STC2 expression by quantitative real-time PCR and found STC2 was upregulated in HCC tissues, correlated with tumor size and multiplicity of HCC. Ectopic expression of STC2 markedly promoted HCC cell proliferation and colony formation, while silencing of endogenous STC2 resulted in a reduced cell growth by cell cycle delay in G0/G1 phase. Western blot analysis demonstrated that STC2 could regulate the expression of cyclin D1 and activate extracellular signal-regulated kinase 1/2 (ERK1/2) in a dominant-positive manner. Transwell chamber assay also indicated altered patterns of STC2 expression had an important effect on cell migration. Our findings suggest that STC2 functions as a potential oncoprotein in the development and progression of HCC as well as a promising molecular target for HCC therapy.
Alterations in hyperpolarization-activated cyclic nucleotide-gated cation channel (HCN) expression in the hippocampus following pilocarpine-induced status epilepticus
Oh, Yun-Jung ; Na, Jongju ; Jeong, Ji-Heon ; Park, Dae-Kyoon ; Park, Kyung-Ho ; Ko, Jeong-Sik ; Kim, Duk-Soo ;
BMB Reports , volume 45, issue 11, 2012, Pages 635~640
DOI : 10.5483/BMBRep.2012.45.11.091
To understand the effects of HCN as potential mediators in the pathogenesis of epilepsy that evoke long-term impaired excitability; the present study was designed to elucidate whether the alterations of HCN expression induced by status epilepticus (SE) is responsible for epileptogenesis. Although HCN1 immunoreactivity was observed in the hippocampus, its immunoreactivities were enhanced at 12 hrs following SE. Although, HCN1 immunoreactivities were reduced in all the hippocampi at 2 weeks, a re-increase in the expression at 2-3 months following SE was observed. In contrast to HCN1, HCN 4 expressions were un-changed, although HCN2 immunoreactive neurons exhibited some changes following SE. Taken together, our findings suggest that altered expressions of HCN1 following SE may be mainly involved in the imbalances of neurotransmissions to hippocampal circuits; thus, it is proposed that HCN1 may play an important role in the epileptogenic period as a compensatory response.
microRNA-214-mediated UBC9 expression in glioma
Zhao, Zhiqiang ; Tan, Xiaochao ; Zhao, Ani ; Zhu, Liyuan ; Yin, Bin ; Yuan, Jiangang ; Qiang, Boqin ; Peng, Xiaozhong ;
BMB Reports , volume 45, issue 11, 2012, Pages 641~646
DOI : 10.5483/BMBRep.2012.45.11.097
It has been reported that ubiquitin-conjugating enzyme 9 (Ubc9), the unique enzyme2 in the sumoylation pathway, is up-regulated in many cancers. However, the expression and regulation of UBC9 in glioma remains unknown. In this study, we found that Ubc9 was up-regulated in glioma tissues and cell lines compared to a normal control. UBC9 knockdown by small interfering RNA (siRNA) affected cell proliferation and apoptosis in T98G cells. Further experiments revealed that microRNA (miR)-214 directly targeted the 3' untranslated region (UTR) of UBC9 and that there was an inverse relationship between the expression levels of miR-214 and UBC9 protein in glioma tissues and cells. miR-214 overexpression suppressed the endogenous UBC9 protein and affected T98G cell proliferation. These findings suggest that miR-214 reduction facilitates UBC9 expression and is involved in the regulation of glioma cell proliferation.
Synergistic inhibition of mesothelioma cell growth by the combination of clofarabine and resveratrol involves Nrf2 downregulation
Lee, Yoon-Jin ; Im, Jae-Hyuk ; Lee, David M. ; Park, Ji-Sung ; Won, Seong Youn ; Cho, Moon-Kyun ; Nam, Hae-Seon ; Lee, Yong-Jin ; Lee, Sang-Han ;
BMB Reports , volume 45, issue 11, 2012, Pages 647~652
DOI : 10.5483/BMBRep.2012.45.11.111
We previously reported that MSTO-211H cells have a higher capacity to regulate Nrf2 activation in response to changes in the cellular redox environment. To further characterize its biological significance, the response of Nrf2, a transcription factor that regulates ARE-containing genes, on the synergistic cytotoxic effect of clofarabine and resveratrol was investigated in mesothelioma cells. The combination treatment showed a marked growth-inhibitory effect, which was accompanied by suppression of Nrf2 activation and decreased expression of heme oxygenase-1 (HO-1). While transient overexpression of Nrf2 conferred protection against the cytotoxicity caused by their combination, knockdown of Nrf2 expression using siRNA enhanced their cytotoxic effect. Pretreatment with Ly294002, a PI3K inhibitor, augmented the decrease in HO-1 level by their combination, whereas no obvious changes were observed in Nrf2 levels. Altogether, these results suggest that the synergistic cytotoxic effect of clofarabine and resveratrol was mediated, at least in part, through suppression of Nrf2 signaling.
Surface glycoproteins determine the feature of the 2009 pandemic H1N1 virus
Kim, Jin Il ; Lee, Ilseob ; Park, Sehee ; Park, Man-Seong ;
BMB Reports , volume 45, issue 11, 2012, Pages 653~658
DOI : 10.5483/BMBRep.2012.45.11.137
After the outbreak of the swine-origin influenza A H1N1 virus in April 2009, World Health Organization declared this novel H1N1 virus as the first pandemic influenza virus (2009 pH1N1) of the
century. To elucidate the characteristics of 2009 pH1N1, the growth properties of A/Korea/01/09 (K/09) was analyzed in cells. Interestingly, the maximal titer of K/09 was higher than that of a seasonal H1N1 virus isolated in Korea 2008 (S/08) though the RNP complex of K/09 was less competent than that of S/08. In addition, the NS1 protein of K/09 was determined as a weak interferon antagonist as compared to that of S/08. Thus, in order to confine genetic determinants of K/09, activities of two major surface glycoproteins were analyzed. Interestingly, K/09 possesses highly reactive NA proteins and weak HA cell-binding avidity. These findings suggest that the surface glycoproteins might be a key factor in the features of 2009 pH1N1.
IFNγ-mediated inhibition of cell proliferation through increased PKCδ-induced overexpression of EC-SOD
Jeon, Yoon-Jae ; Yoo, Hyun ; Kim, Byung Hak ; Lee, Yun Sang ; Jeon, Byeongwook ; Kim, Sung-Sub ; Kim, Tae-Yoon ;
BMB Reports , volume 45, issue 11, 2012, Pages 659~664
DOI : 10.5483/BMBRep.2012.45.11.003
Extracellular superoxide dismutase (EC-SOD) overexpression modulates cellular responses such as tumor cell suppression and is induced by
. Therefore, we examined the role of EC-SOD in
-mediated tumor cell suppression. We observed that the dominant-negative protein kinase C delta (
-induced EC-SOD expression in both keratinocytes and melanoma cells. Our results also showed that
-induced EC-SOD expression was reduced by pretreatment with a PKC-specific inhibitor or a siRNA against
-induced EC-SOD expression suppressed cell proliferations by the up-regulation of p21 and Rb, and the downregulation of cyclin A and D. Finally, we demonstrated that increased expression of EC-SOD drastically suppressed lung melanoma proliferation in an EC-SOD transgenic mouse via p21 expression. In summary, our findings suggest that
-induced EC-SOD expression occurs via activation of
. Therefore, the upregulation of EC-SOD may be effective for prevention of various cancers, including melanoma, via cell cycle arrest.
Shotgun analysis on the peritrophic membrane of the silkworm Bombyx mori
Zhong, Xiaowu ; Zhang, Liping ; Zou, Yong ; Yi, Qiying ; Zhao, Ping ; Xia, Qingyou ; Xiang, Zhonghuai ;
BMB Reports , volume 45, issue 11, 2012, Pages 665~670
DOI : 10.5483/BMBRep.2012.45.11.261
The insect midgut epithelium is generally lined with a unique chitin and protein structure, the peritrophic membrane (PM), which facilitates food digestion and protects the gut epithelium. We used gel electrophoresis and mass spectrometry to identify the extracted proteins from the silkworm PM to obtain an in-depth understanding of the biological function of the silkworm PM components. A total of 305 proteins, with molecular weights ranging from 8.02 kDa to 788.52 kDa and the isoelectric points ranging from 3.39 to 12.91, were successfully identified. We also found several major classes of PM proteins, i.e. PM chitin-binding protein, invertebrate intestinal mucin, and chitin deacetylase. The protein profile provides a basis for further study of the physiological events in the PM of Bombyx mori.
Effects of chronic caloric restriction on kidney and heart redox status and antioxidant enzyme activities in Wistar rats
Dutra, Marcio Ferreira ; Bristot, Ivi Juliana ; Batassini, Cristiane ; Cunha, Nubia Broetto ; Vizuete, Adriana Fernanda Kuckartz ; Souza, Daniela Fraga De ; Moreira, Jose Claudio Fonseca ; Goncalves, Carlos-Alberto ;
BMB Reports , volume 45, issue 11, 2012, Pages 671~676
DOI : 10.5483/BMBRep.2012.45.11.094
Caloric restriction (CR) has been associated with health benefits and these effects have been attributed, in part, to modulation of oxidative status by CR; however, data are still controversial. Here, we investigate the effects of seventeen weeks of chronic CR on parameters of oxidative damage/modification of proteins and on antioxidant enzyme activities in cardiac and kidney tissues. Our results demonstrate that CR induced an increase in protein carbonylation in the heart without changing the content of sulfhydryl groups or the activities of superoxide dismutase and catalase (CAT). Moreover, CR caused an increase in CAT activity in kidney, without changing other parameters. Protein carbonylation has been associated with oxidative damage and functional impairment; however, we cannot exclude the possibility that, under our conditions, this alteration indicates a different functional meaning in the heart tissue. In addition, we reinforce the idea that CR can increase CAT activity in the kidney. Moreover, CR caused an increase in CAT activity in kidney, without changing other parameters. Protein carbonylation has been associated with oxidative damage and functional impairment; however, we cannot exclude the possibility that, under our conditions, this alteration indicates a different functional meaning in the heart tissue. In addition, we reinforce the idea that CR can increase CAT activity in the kidney.