Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal Basic Information
Journal DOI :
Korean Society for Biochemistry and Molecular Biology
Editor in Chief :
Volume & Issues
Volume 46, Issue 12 - Dec 2013
Volume 46, Issue 11 - Nov 2013
Volume 46, Issue 10 - Oct 2013
Volume 46, Issue 9 - Sep 2013
Volume 46, Issue 8 - Aug 2013
Volume 46, Issue 7 - Jul 2013
Volume 46, Issue 6 - Jun 2013
Volume 46, Issue 5 - May 2013
Volume 46, Issue 4 - Apr 2013
Volume 46, Issue 3 - Mar 2013
Volume 46, Issue 2 - Feb 2013
Volume 46, Issue 1 - Jan 2013
Selecting the target year
Biochemistry and structure of phosphoinositide phosphatases
Kim, Young Jun ; Jahan, Nusrat ; Bahk, Young Yil ;
BMB Reports , volume 46, issue 1, 2013, Pages 1~8
DOI : 10.5483/BMBRep.2013.46.1.261
Phosphoinositides are the phosphorylated derivatives of phosphatidylinositol, and play a very significant role in a diverse range of signaling processes in eukaryotic cells. A number of phosphoinositide-metabolizing enzymes, including phosphoinositide-kinases and phosphatases are involved in the synthesis and degradation of these phospholipids. Recently, the function of various phosphatases in the phosphatidylinositol signaling pathway has been of great interest. In the present review we summarize the structural insights and biochemistry of various phosphatases in regulating phosphoinositide metabolism.
When a ribosome encounters a premature termination codon
Hwang, Jungwook ; Kim, Yoon Ki ;
BMB Reports , volume 46, issue 1, 2013, Pages 9~16
DOI : 10.5483/BMBRep.2013.46.1.002
In mammalian cells, aberrant transcripts harboring a premature termination codon (PTC) can be generated by abnormal or inefficient biogenesis of mRNAs or by somatic mutation. Truncated polypeptides synthesized from these aberrant transcripts could be toxic to normal cellular functions. However, mammalian cells have evolved sophisticated mechanisms for monitoring the quality of mRNAs. The faulty transcripts harboring PTC are subject to nonsense-mediated mRNA decay (NMD), nonsense-mediated translational repression (NMTR), nonsense-associated alternative splicing (NAS), or nonsense-mediated transcriptional gene silencing (NMTGS). In this review, we briefly outline the molecular characteristics of each pathway and suggest mRNA quality control mechanisms as a means to regulate normal gene expression.
Transcutaneous antigen delivery system
Lee, Mi-Young ; Shin, Meong-Cheol ; Yang, Victor C. ;
BMB Reports , volume 46, issue 1, 2013, Pages 17~24
DOI : 10.5483/BMBRep.2013.46.1.001
Transcutaneous immunization refers to the topical application of antigens onto the epidermis. Transcutaneous immunization targeting the Langerhans cells of the skin has received much attention due to its safe, needle-free, and noninvasive antigen delivery. The skin has important immunological functions with unique roles for antigen-presenting cells such as epidermal Langerhans cells and dermal dendritic cells. In recent years, novel vaccine delivery strategies have continually been developed; however, transcutaneous immunization has not yet been fully exploited due to the penetration barrier represented by the stratum corneum, which inhibits the transport of antigens and adjuvants. Herein we review recent achievements in transcutaneous immunization, focusing on the various strategies for the enhancement of antigen delivery and vaccination efficacy.
Connexin32 inhibits gastric carcinogenesis through cell cycle arrest and altered expression of p21
Jee, Hyang ; Lee, Su-Hyung ; Park, Jun-Won ; Lee, Bo-Ram ; Nam, Ki-Taek ; Kim, Dae-Yong ;
BMB Reports , volume 46, issue 1, 2013, Pages 25~30
DOI : 10.5483/BMBRep.2013.46.1.078
Gap junctions and their structural proteins, connexins (Cxs), have been implicated in carcinogenesis. To explore the involvement of Cx32 in gastric carcinogenesis, immunochemical analysis of Cx32 and proliferation marker Ki67 using tissue-microarrayed human gastric cancer and normal tissues was performed. In addition, after Cx32 overexpression in the human gastric cancer cell line AGS, cell proliferation, cell cycle analyses, and
expression levels were examined by bromodeoxyuridine assay, flow cytometry, real-time RT-PCR, and western blotting. Immunohistochemical study noted a strong inverse correlation between Cx32 and Ki67 expression pattern as well as their location. In vitro, overexpression of Cx32 in AGS cells inhibited cell proliferation significantly.
arrest, up-regulation of cell cycle-regulatory proteins
was also found at both mRNA and protein levels. Taken together, Cx32 plays some roles in gastric cancer development by inhibiting gastric cancer cell proliferation through cell cycle arrest and cell cycle regulatory proteins.
Over-expression of OsHsfA7 enhanced salt and drought tolerance in transgenic rice
Liu, Ai-Ling ; Zou, Jie ; Liu, Cui-Fang ; Zhou, Xiao-Yun ; Zhang, Xian-Wen ; Luo, Guang-Yu ; Chen, Xin-Bo ;
BMB Reports , volume 46, issue 1, 2013, Pages 31~36
DOI : 10.5483/BMBRep.2013.46.1.090
Heat shock proteins play an important role in plant stress tolerance and are mainly regulated by heat shock transcription factors (Hsfs). In this study, we generated transgenic rice over-expressing OsHsfA7 and carried out morphological observation and stress tolerance assays. Transgenic plants exhibited less, shorter lateral roots and root hair. Under salt treatment, over-expressing OsHsfA7 rice showed alleviative appearance of damage symptoms and higher survival rate, leaf electrical conductivity and malondialdehyde content of transgenic plants were lower than those of wild type plants. Meanwhile, transgenic rice seedlings restored normal growth but wild type plants could not be rescued after drought and re-watering treatment. These findings indicate that over-expression of OsHsfA7 gene can increase tolerance to salt and drought stresses in rice seedlings.
Altered sugar donor specificity and catalytic activity of pteridine glycosyltransferases by domain swapping or site-directed mutagenesis
Kim, Hye-Lim ; Kim, Ae Hyun ; Park, Mi Bi ; Lee, Soo-Woong ; Park, Young Shik ;
BMB Reports , volume 46, issue 1, 2013, Pages 37~40
DOI : 10.5483/BMBRep.2013.46.1.147
CY-007 and CY-049 pteridine glycosyltransferases (PGTs) that differ in sugar donor specificity to catalyze either glucose or xylose transfer to tetrahydrobiopterin were studied here to uncover the structural determinants necessary for the specificity. The importance of the C-terminal domain and its residues 218 and 258 that are different between the two PGTs was assessed via structure-guided domain swapping or single and dual amino acid substitutions. Catalytic activity and selectivity were altered in all the mutants (2 chimeric and 6 substitution) to accept both UDP-glucose and UDP-xylose. In addition, the wild type activities were improved 1.6-4.2 fold in 4 substitution mutants and activity was observed towards another substrate UDP-N-acetylglucosamine in all the substitution mutants from CY-007 PGT. The results strongly support essential role of the C-terminal domain and the two residues for catalysis as well as sugar donor specificity, bringing insight into the structural features of the PGTs.
Partial AUC maximization for essential gene prediction using genetic algorithms
Hwang, Kyu-Baek ; Ha, Beom-Yong ; Ju, Sanghun ; Kim, Sangsoo ;
BMB Reports , volume 46, issue 1, 2013, Pages 41~46
DOI : 10.5483/BMBRep.2013.46.1.159
Identifying genes indispensable for an organism's life and their characteristics is one of the central questions in current biological research, and hence it would be helpful to develop computational approaches towards the prediction of essential genes. The performance of a predictor is usually measured by the area under the receiver operating characteristic curve (AUC). We propose a novel method by implementing genetic algorithms to maximize the partial AUC that is restricted to a specific interval of lower false positive rate (FPR), the region relevant to follow-up experimental validation. Our predictor uses various features based on sequence information, protein-protein interaction network topology, and gene expression profiles. A feature selection wrapper was developed to alleviate the over-fitting problem and to weigh each feature's relevance to prediction. We evaluated our method using the proteome of budding yeast. Our implementation of genetic algorithms maximizing the partial AUC below 0.05 or 0.10 of FPR outperformed other popular classification methods.
Heat shock protein 90β inhibits apoptosis of intestinal epithelial cells induced by hypoxia through stabilizing phosphorylated Akt
Zhang, Shuai ; Sun, Yong ; Yuan, Zhiqiang ; Li, Ying ; Li, Xiaolu ; Gong, Zhenyu ; Peng, Yizhi ;
BMB Reports , volume 46, issue 1, 2013, Pages 47~52
DOI : 10.5483/BMBRep.2013.46.1.037
Intestinal epithelial cell (IEC) apoptosis induced by hypoxia compromise intestinal epithelium barrier function. Both Akt and Hsp90 have cytoprotective function. However, the specific role of Akt and
in IEC apoptosis induced by hypoxia has not been explored. We confirmed that hypoxia-induced apoptosis was reduced by
overexpression but enhanced by decreasing
overexpression enhanced BAD phosphorylation and thus reduced mitochondrial release of cytochrome C. Reducing
expression had opposite effects. The protective effect of
against apoptosis was negated by LY294002, an Akt inhibitor. Further study showed that Akt phosphorylation was enhanced by
, which was not due to the activation of upstream PI3K and PDK1 but because of stabilization of pAkt via direct interaction between
and pAkt. These results demonstrate that
may play a significant role in protecting IECs from hypoxia-induced apoptosis via stabilizing pAkt to phosphorylate BAD and reduce cytochrome C release.
Biochemical characteristics of functional domains using feline foamy virus integrase mutants
Yoo, Gwi-Woong ; Shin, Cha-Gyun ;
BMB Reports , volume 46, issue 1, 2013, Pages 53~58
DOI : 10.5483/BMBRep.2013.46.1.118
We constructed deletion mutants and seven point mutants by polymerase chain reaction to investigate the specificity of feline foamy virus integrase functional domains. Complementation reactions were performed for three enzymatic activities such as 3'-end processing, strand transfer, and disintegration. The complementation reactions with deletion mutants showed several activities for 3'-end processing and strand transfer. The conserved central domain and the combination of the N-terminal or C-terminal domains increased disintegration activity significantly. In the complementation reactions between deletion and point mutants, the combination between D107V and deletion mutants revealed 3'-end processing activities, but the combination with others did not have any activity, including strand transfer activities. Disintegration activity increased evenly, except the combination with glutamic acid 200. These results suggest that an intact central domain mediates enzymatic activities but fails to show these activities in the absence of the N-terminal or C-terminal domains.
Antiproliferative effect of gold(I) compound auranofin through inhibition of STAT3 and telomerase activity in MDA-MB 231 human breast cancer cells
Kim, Nam-Hoon ; Park, Hyo Jung ; Oh, Mi-Kyung ; Kim, In-Sook ;
BMB Reports , volume 46, issue 1, 2013, Pages 59~64
DOI : 10.5483/BMBRep.2013.46.1.123
Signal transducer and activator of transcription 3 (STAT3) and telomerase are considered attractive targets for anticancer therapy. The in vitro anticancer activity of the gold(I) compound auranofin was investigated using MDA-MB 231 human breast cancer cells, in which STAT3 is constitutively active. In cell culture, auranofin inhibited growth in a dose-dependent manner, and N-acetyl-L-cysteine (NAC), a scavenger of reactive oxygen species (ROS), markedly blocked the effect of auranofin. Incorporation of 5-bromo-2'-deoxyuridine into DNA and anchorage-independent cell growth on soft agar were decreased by auranofin treatment. STAT3 phosphorylation and telomerase activity were also attenuated in cells exposed to auranofin, but NAC pretreatment restored STAT3 phosphorylation and telomerase activity in these cells. These findings indicate that auranofin exerts in vitro antitumor effects in MDA-MB 231 cells and its activity involves inhibition of STAT3 and telomerase. Thus, auranofin shows potential as a novel anticancer drug that targets STAT3 and telomerase.