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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biochemistry and Molecular Biology
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Volume & Issues
Volume 47, Issue 12 - Dec 2014
Volume 47, Issue 11 - Nov 2014
Volume 47, Issue 10 - Oct 2014
Volume 47, Issue 9 - Sep 2014
Volume 47, Issue 8 - Aug 2014
Volume 47, Issue 7 - Jul 2014
Volume 47, Issue 6 - Jun 2014
Volume 47, Issue 5 - May 2014
Volume 47, Issue 4 - Apr 2014
Volume 47, Issue 3 - Mar 2014
Volume 47, Issue 2 - Feb 2014
Volume 47, Issue 1 - Jan 2014
Selecting the target year
4-1BB (CD137), an inducible costimulatory receptor, as a specific target for cancer therapy
Vinay, Dass S. ; Kwon, Byoung S. ;
BMB Reports , volume 47, issue 3, 2014, Pages 122~129
DOI : 10.5483/BMBRep.2014.47.3.283
Although considerable progress has been made in understanding how tumors evade immune surveillance, measures to counter the same have not kept pace with the advances made in designing effective strategies. 4-1BB (CD137; TNFRS9), an activation-induced costimulatory molecule, is an important regulator of immune responses. Targeting 4-1BB or its natural ligand 4-1BB ligand (4-1BBL) has important implications in many clinical conditions, including cancer. In-depth analysis revealed that 4-1BB-mediated anti-cancer effects are based on its ability to induce activation of cytotoxic T lymphocytes (CTL), and among others, high amounts of IFN-
. In this review, we will discuss the various aspects of 4-1BB-mediated anti-tumor responses, the basis of such responses, and future directions.
In vitro and in vivo application of anti-cotinine antibody and cotinine-conjugated compounds
Kim, Hyori ; Yoon, Soomin ; Chung, Junho ;
BMB Reports , volume 47, issue 3, 2014, Pages 130~134
DOI : 10.5483/BMBRep.2014.47.3.006
The combination of a high-affinity antibody to a hapten, and hapten-conjugated compounds, can provide an alternative to the direct chemical cross-linking of the antibody and compounds. An optimal hapten for in vitro use is one that is absent in biological systems. For in vivo applications, additional characteristics such as pharmacological safety and physiological inertness would be beneficial. Additionally, methods for cross-linking the hapten to various chemical compounds should be available. Cotinine, a major metabolite of nicotine, is considered advantageous in these aspects. A high-affinity anti-cotinine recombinant antibody has recently become available, and can be converted into various formats, including a bispecific antibody. The bispecific anti-cotinine antibody was successfully applied to immunoblot, enzyme immunoassay, immunoaffinity purification, and pre-targeted in vivo radioimmunoimaging. The anti-cotinine IgG molecule could be complexed with aptamers to form a novel affinity unit, and extended the in vivo half-life of aptamers, opening up the possibility of applying the same strategy to therapeutic peptides and chemical compounds.
The potential of mesenchymal stem cells derived from amniotic membrane and amniotic fluid for neuronal regenerative therapy
Kim, Eun Young ; Lee, Kyung-Bon ; Kim, Min Kyu ;
BMB Reports , volume 47, issue 3, 2014, Pages 135~140
DOI : 10.5483/BMBRep.2014.47.3.289
The mesenchymal stem cells (MSCs), which are derived from the mesoderm, are considered as a readily available source for tissue engineering. They have multipotent differentiation capacity and can be differentiated into various cell types. Many studies have demonstrated that the MSCs identified from amniotic membrane (AM-MSCs) and amniotic fluid (AF-MSCs) are shows advantages for many reasons, including the possibility of noninvasive isolation, multipotency, self-renewal, low immunogenicity, anti-inflammatory and nontumorigenicity properties, and minimal ethical problem. The AF-MSCs and AM-MSCs may be appropriate sources of mesenchymal stem cells for regenerative medicine, as an alternative to embryonic stem cells (ESCs). Recently, regenerative treatments such as tissue engineering and cell transplantation have shown potential in clinical applications for degenerative diseases. Therefore, amnion and MSCs derived from amnion can be applied to cell therapy in neuro-degeneration diseases. In this review, we will describe the potential of AM-MSCs and AF-MSCs, with particular focus on cures for neuronal degenerative diseases.
Proteomics approaches for the studies of bone metabolism
Lee, Ji-Hyun ; Cho, Je-Yoel ;
BMB Reports , volume 47, issue 3, 2014, Pages 141~148
DOI : 10.5483/BMBRep.2014.47.3.270
Bone is an active tissue, in which bone formation by osteoblast is followed by bone resorption by osteoclasts, in a repeating cycle. Proteomics approaches may allow the detection of changes in cell signal transduction, and the regulatory mechanism of cell differentiation. LC-MS/MS-based quantitative methods can be used with labeling strategies, such as SILAC, iTRAQ, TMT and enzymatic labeling. When used in combination with specific protein enrichment strategies, quantitative proteomics methods can identify various signaling molecules and modulators, and their interacting proteins in bone metabolism, to elucidate biological functions for the newly identified proteins in the cellular context. In this article, we will briefly review recent major advances in the application of proteomics for bone biology, especially from the aspect of cellular signaling.
Small-molecule probes elucidate global enzyme activity in a proteomic context
Lee, Jun-Seok ; Yoo, Young-Hwa ; Yoon, Chang No ;
BMB Reports , volume 47, issue 3, 2014, Pages 149~157
DOI : 10.5483/BMBRep.2014.47.3.264
The recent dramatic improvements in high-resolution mass spectrometry (MS) have revolutionized the speed and scope of proteomic studies. Conventional MS-based proteomics methodologies allow global protein profiling based on expression levels. Although these techniques are promising, there are numerous biological activities yet to be unveiled, such as the dynamic regulation of enzyme activity. Chemical proteomics is an emerging field that extends these types proteomic profiling. In particular, activity-based protein profiling (ABPP) utilizes small-molecule probes to monitor enzyme activity directly in living intact subjects. In this mini-review, we summarize the unique roles of smallmolecule probes in proteomics studies and highlight some recent examples in which this principle has been applied.
Tumor bioenergetics: An emerging avenue for cancer metabolism targeted therapy
Kee, Hyun Jung ; Cheong, Jae-Ho ;
BMB Reports , volume 47, issue 3, 2014, Pages 158~166
DOI : 10.5483/BMBRep.2014.47.3.273
Cell proliferation is a delicately regulated process that couples growth signals and metabolic demands to produce daughter cells. Interestingly, the proliferation of tumor cells immensely depends on glycolysis, the Warburg effect, to ensure a sufficient amount of metabolic flux and bioenergetics for macromolecule synthesis and cell division. This unique metabolic derangement would provide an opportunity for developing cancer therapeutic strategy, particularly when other diverse anti-cancer treatments have been proved ineffective in achieving durable response, largely due to the emergence of resistance. Recent advances in deeper understanding of cancer metabolism usher in new horizons of the next generation strategy for cancer therapy. Here, we discuss the focused review of cancer energy metabolism, and the therapeutic exploitation of glycolysis and OXPHOS as a novel anti-cancer strategy, with particular emphasis on the promise of this approach, among other cancer metabolism targeted therapies that reveal unexpected complexity and context-dependent metabolic adaptability, complicating the development of effective strategies.
Structural insights into the transcription-independent apoptotic pathway of p53
Chi, Seung-Wook ;
BMB Reports , volume 47, issue 3, 2014, Pages 167~172
DOI : 10.5483/BMBRep.2014.47.3.261
Reactivating the p53 pathway in tumors is an important strategy for anticancer therapy. In response to diverse cellular stresses, the tumor suppressor p53 mediates apoptosis in a transcription-independent and transcription-dependent manner. Although extensive studies have focused on the transcription-dependent apoptotic pathway of p53, the transcription-independent apoptotic pathway of p53 has only recently been discovered. Molecular interactions between p53 and Bcl-2 family proteins in the mitochondria play an essential role in the transcription-independent apoptosis of p53. This review describes the structural basis for the transcription-independent apoptotic pathway of p53 and discusses its potential application to anticancer therapy.
Perspectives on the therapeutic potential of short-chain fatty acid receptors
Kim, Sunhong ; Kim, Jeong-Hoon ; Park, Bi Oh ; Kwak, Young Shin ;
BMB Reports , volume 47, issue 3, 2014, Pages 173~178
DOI : 10.5483/BMBRep.2014.47.3.272
There is rapidly growing interest in the human microbiome because of its implication in metabolic disorders and inflammatory diseases. Consequently, understanding the biology of short chain fatty acids and their receptors has become very important for identifying novel therapeutic avenues. GPR41 and GPR43 have been recognized as the cognate receptors for SCFAs and their roles in metabolism and inflammation have drawn much attention in recent years. GPR43 is highly expressed on immune cells and has been suggested to play a role in inflammatory diseases such as inflammatory bowel disease. Both GPR41 and GPR43 have been implicated in diabetes and obesity via the regulation of adipose tissue and gastrointestinal hormones. So far, many studies have provided contradictory results, and therefore further research is required to validate these receptors as drug targets. We will also discuss the synthetic modulators of GPR41 and GPR43 that are critical to understanding the functions of these receptors.