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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal Basic Information
Journal DOI :
Korean Society for Biochemistry and Molecular Biology
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Volume & Issues
Volume 47, Issue 12 - Dec 2014
Volume 47, Issue 11 - Nov 2014
Volume 47, Issue 10 - Oct 2014
Volume 47, Issue 9 - Sep 2014
Volume 47, Issue 8 - Aug 2014
Volume 47, Issue 7 - Jul 2014
Volume 47, Issue 6 - Jun 2014
Volume 47, Issue 5 - May 2014
Volume 47, Issue 4 - Apr 2014
Volume 47, Issue 3 - Mar 2014
Volume 47, Issue 2 - Feb 2014
Volume 47, Issue 1 - Jan 2014
Selecting the target year
Individual and collective responsibility to enhance regulatory compliance of the Three Rs
Choe, Byung In ; Lee, Gwi Hyang ;
BMB Reports , volume 47, issue 4, 2014, Pages 179~183
DOI : 10.5483/BMBRep.2014.47.4.049
Investigators planning to use animals in their research and the Institutional Animal Care and Use Committee (IACUC) members who review the research protocols must take personal responsibility for ensuring that they have the skills and knowledge to perform their duties, applying the Three Rs principles of Russell and Burch. The two Korean laws introduced in 2008 and 2009 regulating animal use for scientific purposes in line with the Three Rs principles have been revised a total of 11 times over the last 6 years. Both regulatory agencies, e.g., the Animal and Plant Quarantine Agency and the Ministry of Food and Drug Safety, provide regular training based on the legal requirements. Based on the amended Animal Welfare Act, the IACUC appointment framework has been upgraded: appointments are now for two-year terms and require a qualified training certificate issued by the Animal and Plant Quarantine Agency since 2012. The authors reviewed the current curricular programs and types of training conducted by the two governing agencies through Internet searches. Our Internet survey results suggest that: a) diversity should be provided in training curricula, based on the roles, backgrounds and needs of the individual trainees; b) proper and continued educational programs should be provided, based on trainees' experiences; and c) active encouragement by government authorities can improve the quality of training curricula.
Innate immune recognition of respiratory syncytial virus infection
Kim, Tae Hoon ; Lee, Heung Kyu ;
BMB Reports , volume 47, issue 4, 2014, Pages 184~191
DOI : 10.5483/BMBRep.2014.47.4.050
Respiratory syncytial virus (RSV) is the leading cause of respiratory infection in infants and young children. Severe clinical manifestation of RSV infection is a bronchiolitis, which is common in infants under six months of age. Recently, RSV has been recognized as an important cause of respiratory infection in older populations with cardiovascular morbidity or immunocompromised patients. However, neither a vaccine nor an effective antiviral therapy is currently available. Moreover, the interaction between the host immune system and the RSV pathogen during an infection is not well understood. The innate immune system recognizes RSV through multiple mechanisms. The first innate immune RSV detectors are the pattern recognition receptors (PRRs), including toll-like receptors (TLRs), retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs), and nucleotide-biding oligomerization domain (NOD)-like receptors (NLRs). The following is a review of studies associated with various PRRs that are responsible for RSV virion recognition and subsequent induction of the antiviral immune response during RSV infection.
The diverse roles of RNA polymerase II C-terminal domain phosphatase SCP1
Harikrishna, Reddy R. ; Kim, Hackyoung ; Noh, Kwangmo ; Kim, Young Jun ;
BMB Reports , volume 47, issue 4, 2014, Pages 192~196
DOI : 10.5483/BMBRep.2014.47.4.060
RNA polymerase II carboxyl-terminal domain (pol II CTD) phosphatases are a newly emerging family of phosphatases that are members of DXDX (T/V). The subfamily includes Small CTD phosphatases, like SCP1, SCP2, SCP3, TIMM50, HSPC129 and UBLCP. Extensive study of SCP1 has elicited the diversified roles of the small C terminal domain phosphatase. The SCP1 plays a vital role in various biological activities, like neuronal gene silencing and preferential Ser5 dephosphorylation, acts as a cardiac hypertrophy inducer with the help of its intronic miRNAs, and has shown a key role in cell cycle regulation. This short review offers an explanation of the mechanism of action of small CTD phosphatases, in different biological activities and metabolic processes.
Cyclic AMP response element binding (CREB) protein acts as a positive regulator of SOX3 gene expression in NT2/D1 cells
Kovacevic-Grujicic, Natasa ; Mojsin, Marija ; Popovic, Jelena ; Petrovic, Isidora ; Topalovic, Vladanka ; Stevanovic, Milena ;
BMB Reports , volume 47, issue 4, 2014, Pages 197~202
DOI : 10.5483/BMBRep.2014.47.4.084
SOX3 is one of the earliest neural markers in vertebrates, playing the role in specifying neuronal fate. In this study we have established first functional link between CREB and human SOX3 gene which both have important roles in the nervous system throughout development and in the adulthood. Here we demonstrate both in vitro and in vivo that CREB binds to CRE half-site located -195 to -191 within the human SOX3 promoter. Overexpression studies with CREB or its dominant-negative inhibitor A-CREB indicate that this transcription factor acts as a positive regulator of basal SOX3 gene expression in NT2/D1 cells. This is further confirmed by mutational analysis where mutation of CREB binding site results in reduction of SOX3 promoter activity. Our results point at CREB as a positive regulator of SOX3 gene transcription in NT2/D1 cells, while its contribution to RA induction of SOX3 promoter is not prominent.
siRNA-mediated gene silencing of MexB from the MexA-MexB-OprM efflux pump in Pseudomonas aeruginosa
Gong, Feng-Yun ; Zhang, Ding-Yu ; Zhang, Jiang-Guo ; Wang, Li-Li ; Zhan, Wei-Li ; Qi, Jun-Ying ; Song, Jian-Xin ;
BMB Reports , volume 47, issue 4, 2014, Pages 203~208
DOI : 10.5483/BMBRep.2014.47.4.040
To gain insights into the effect of MexB gene under the short interfering RNA (siRNA), we synthesized 21 bp siRNA duplexes against the MexB gene. RT-PCR was performed to determine whether the siRNA inhibited the expression of MexB mRNA. Changes in antibiotic susceptibility in response to siRNA were measured by the E-test method. The efficacy of siRNAs was determined in a murine model of chronic P. aeruginosa lung infection. MexB-siRNAs inhibited both mRNA expression and the activity of P. aeruginosa in vitro. In vivo, siRNA was effective in reducing the bacterial load in the model of chronic lung infection and the P. aeruginosa-induced pathological changes. MexB-siRNA treatment enhanced the production of inflammatory cytokines in the early infection stage (P < 0.05). Our results suggest that targeting of MexB with siRNA appears to be a novel strategy for treating P. aeruginosa infections.
Enhancement of UVB radiation-mediated apoptosis by knockdown of cytosolic NADP
-dependent isocitrate dehydrogenase in HaCaT cells
Lee, Su Jeong ; Park, Jeen-Woo ;
BMB Reports , volume 47, issue 4, 2014, Pages 209~214
DOI : 10.5483/BMBRep.2014.47.4.137
Ultraviolet B (UVB) radiation induces the production of reactive oxygen species (ROS) that promote apoptotic cell death. We showed that cytosolic
-dependent isocitrate dehydrogenase (IDPc) plays an essential role in the control of cellular redox balance and defense against oxidative damage, by supplying NADPH for antioxidant systems. In this study, we demonstrated that knockdown of IDPc expression by RNA interference enhances UVB-induced apoptosis of immortalized human HaCaT keratinocytes. This effect manifested as DNA fragmentation, changes in cellular redox status, mitochondrial dysfunction, and modulation of apoptotic marker expression. Based on our findings, we suggest that attenuation of IDPc expression may protect skin from UVB-mediated damage, by inducing the apoptosis of UV-damaged cells.
Therapeutic effect of a TM4SF5-specific peptide vaccine against colon cancer in a mouse model
Kwon, Sanghoon ; Kim, Young-Eun ; Park, Jeong-A ; Kim, Doo-Sik ; Kwon, Hyung-Joo ; Lee, Younghee ;
BMB Reports , volume 47, issue 4, 2014, Pages 215~220
DOI : 10.5483/BMBRep.2014.47.4.157
Molecular-targeted therapy has gained attention because of its high efficacy and weak side effects. Previously, we confirmed that transmembrane 4 superfamily member 5 protein (TM4SF5) can serve as a molecular target to prevent or treat hepatocellular carcinoma (HCC). We recently extended the application of the peptide vaccine, composed of CpG-DNA, liposome complex, and TM4SF5 peptide, to prevent colon cancer in a mouse model. Here, we first implanted mice with mouse colon cancer cells and then checked therapeutic effects of the vaccine against tumor growth. Immunization with the peptide vaccine resulted in robust production of TM4SF5-specific antibodies, alleviated tumor growth, and reduced survival rate of the tumor-bearing mice. We also found that serum levels of VEGF were markedly reduced in the mice immunized with the peptide vaccine. Therefore, we suggest that the TM4SF5-specific peptide vaccine has a therapeutic effect against colon cancer in a mouse model.
Nutlin-3 downregulates p53 phosphorylation on serine
and induces apoptosis in hepatocellular carcinoma cells
Shi, Xinli ; Liu, Jingli ; Ren, Laifeng ; Mao, Nan ; Tan, Fang ; Ding, Nana ; Yang, Jing ; Li, Mingyuan ;
BMB Reports , volume 47, issue 4, 2014, Pages 221~226
DOI : 10.5483/BMBRep.2014.47.4.146
Drug-resistance and imbalance of apoptotic regulation limit chemotherapy clinical application for the human hepatocellular carcinoma (HCC) treatment. The reactivation of p53 is an attractive therapeutic strategy in cancer with disrupted-p53 function. Nutlin-3, a MDM2 antagonist, has antitumor activity in various cancers. The post-translational modifications of p53 are a hot topic, but there are some controversy ideas about the function of phospho-
-p53 protein in cancer cell lines in response to Nutlin-3. Therefore, we investigated the relationship between Nutlin-3 and phospho-
-p53 protein expression levels in SMMC-7721 (wild-type TP53) and HuH-7 cells (mutant TP53). We demonstrated that Nutlin-3 induced apoptosis through down-regulation phospho-
-p53 in two HCC cells. The result suggests that inhibition of p53 phosphorylation on
presents an alternative for HCC chemotherapy.
HDAC3 acts as a negative regulator of angiogenesis
Park, Deokbum ; Park, Hyunmi ; Kim, Youngmi ; Kim, Hyuna ; Jeoung, Dooil ;
BMB Reports , volume 47, issue 4, 2014, Pages 227~232
DOI : 10.5483/BMBRep.2014.47.4.128
Histone deacetylase-3 (HDAC3) is involved in cellular proliferation, apoptosis and transcriptional repression. However, the role of HDAC3 in angiogenesis remains unknown. HDAC3 negatively regulated the expression of angiogenic factors, such as VEGF and plasminogen activator inhibitor-1 (PAI-1). HDAC3 showed binding to promoter sequences of PAI-1. HDAC3 activity was necessary for the expression regulation of PAI-1 by HDAC3. VEGF decreased the expression of HDAC3, and the down-regulation of HDAC3 enhanced endothelial cell tube formation. HDAC3 negatively regulated tumor-induced angiogenic potential. We show the novel role of HDAC3 as a negative regulator of angiogenesis.
Characterization of a novel posttranslational modification in polypyrimidine tract-binding proteins by SUMO1
Han, Wei ; Wang, Lin ; Yin, Bin ; Peng, Xiaozhong ;
BMB Reports , volume 47, issue 4, 2014, Pages 233~238
DOI : 10.5483/BMBRep.2014.47.4.140
Polypyrimidine tract-binding protein 1 (PTBP1) and its brain-specific homologue, PTBP2, are associated with pre-mRNAs and influence pre-mRNA processing, as well as mRNA metabolism and transport. They play important roles in neural differentiation and glioma development. In our study, we detected the expression of the two proteins in glioma cells and predicted that they may be sumoylated using SUMOplot analyses. We confirmed that PTBP1 and PTBP2 can be modified by SUMO1 with co-immunoprecipitation experiments using 293ET cells transiently co-expressing SUMO1 and either PTBP1 or PTBP2. We also found that SUMO1 modification of PTBP2 was enhanced by Ubc9 (E2). The mutation of the sumoylation site (Lys137) of PTBP2 markedly inhibited its modification by SUMO1. Interestingly, in T98G glioma cells, the level of sumoylated PTBP2 was reduced compared to that of normal brain cells. Overall, this study shows that PTBP2 is posttranslationally modified by SUMO1.