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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Mass Spectrometry Letters
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Journal DOI :
Korean Society Mass Spectrometry
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Volume & Issues
Volume 3, Issue 4 - Dec 2012
Volume 3, Issue 3 - Sep 2012
Volume 3, Issue 2 - Jun 2012
Volume 3, Issue 1 - Mar 2012
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Gas Chromatography-High Resolution Tandem Mass Spectrometry Using a GC-APPI-LIT Orbitrap for Complex Volatile Compounds Analysis
Lee, Young-Jin ; Smith, Erica A. ; Jun, Ji-Hyun ;
Mass Spectrometry Letters, volume 3, issue 2, 2012, Pages 29~38
DOI : 10.5478/MSL.2012.3.2.29
A new approach of volatile compounds analysis is proposed using a linear ion trap Orbitrap mass spectrometer coupled with gas chromatography through an atmospheric pressure photoionization interface. In the proposed GC-HRMS/MS approach, direct chemical composition analysis is made for the precursor ions in high resolution MS spectra and the structural identifications were made through the database search of high quality MS/MS spectra. Successful analysis of a complex perfume sample was demonstrated and compared with GC-EI-Q and GC-EI-TOF. The current approach is complementary to conventional GC-EI-MS analysis and can identify low abundance co-eluting compounds. Toluene co-sprayed as a dopant through API probe significantly enhanced ionization of certain compounds and reduced oxidation during the ionization.
An Application of Electrostatic Repulsion Hydrophilic Interaction Chromatography in Phospho- and Glycoproteome Profiling of Epicardial Adipose Tissue in Obesity Mouse
Tran, Trang Huyen ; Hwang, In-Jae ; Park, Jong-Moon ; Kim, Jae-Bum ; Lee, Hoo-Keun ;
Mass Spectrometry Letters, volume 3, issue 2, 2012, Pages 39~42
DOI : 10.5478/MSL.2012.3.2.39
Phosphorylation and glycosylation are two of the most important and widespread post-translational modifications (PTMs) in an organism. Proteomics analysis of the PTMs has been challenged by low stoichiometry of the modified proteins and suppression effects by high abundance proteins, typically no-functional house-keeping proteins. In this study, a novel method was applied for not only isolating PTM peptides from intact peptides but also concurrently characterizing of glyco- and phosphoproteome using electrostatic repulsion hydrophilic interaction chromatography (ERLIC) packed with silica coated by crosslinked polyethyleneimine. For 2 mg tryptic digest of mouse proteome of epicardial adipose tissue with fat diet, 802 N-glycosylated peptides of 316 glycoproteins and 159 phosphorylated peptides of 75 phosphoproteins were identified using HPLC chip/quadrupole time-of-flight (Q-OF) tandem mass spectrometer.
Application of Comprehensive 2D GC-MS and APPI FT-ICR MS for More Complete Understanding of Chemicals in Diesel Fuel
Cho, Yun-Ju ; Islam, Annana ; Ahmed, Arif ; Kim, Sung-Hwan ;
Mass Spectrometry Letters, volume 3, issue 2, 2012, Pages 43~46
DOI : 10.5478/MSL.2012.3.2.43
In this study, comprehensive two dimension gas chromatography (2D GC-MS) and 15 T Fourier transform ion cyclotron resonance mass spectrometry (15T FT-ICR MS) connected to atmospheric pressure photo ionization (APPI) have been combined to obtain detailed chemical composition of a diesel oil sample. With 2D GC-MS, compounds with aliphatic alkyl, saturated cyclic ring(s), and one aromatic ring structures were mainly identified. Sensitivity toward aromatic compounds with more than two aromatic rings was low with 2D GC-MS. In contrast, aromatic compounds containing up to four benzene rings were identified by APPI FT-ICR MS. Relatively smaller abundance of cyclic ring compounds were found but no aliphatic alkyl compounds were observed by APPI FT-ICR MS. The data presented in this study clearly shows that 2D GC-MS and 15T FT-ICR MS provides different aspect of an oil sample and hence they have to be considered as complementary techniques to each other for more complete understanding of oil samples.
Microwave-assisted Weak Acid Hydrolysis of Proteins
Seo, Mi-Yeong ; Kim, Jin-Hee ; Park, Se-Hwan ; Lee, Ji-Hye ; Kim, Tae-Hee ; Lee, Ji-Hyeon ; Kim, Jeong-Kwon ;
Mass Spectrometry Letters, volume 3, issue 2, 2012, Pages 47~49
DOI : 10.5478/MSL.2012.3.2.47
Myoglobin was hydrolyzed by microwave-assisted weak acid hydrolysis with 2% formic acid at
for 1 h. The most effective hydrolysis was observed at
. Hydrolysis products were investigated using matrixassisted laser desorption/ionization time-of-flight mass spectrometry. Most cleavages predominantly occurred at the C-termini of aspartyl residues. For comparison, weak acid hydrolysis was also performed in boiling water for 20, 40, 60, and 120 min. A 60- min weak acid hydrolysis in boiling water yielded similar results as a 60-min microwave-assisted weak acid hydrolysis at
. These results strongly suggest that microwave irradiation has no notable enhancement effect on acid hydrolysis of proteins and that temperature is the major factor that determines the effectiveness of weak acid hydrolysis.
Simultaneous Determination of Synthetic Phosphodiesterase-5 Inhibitors in Dietary Supplements by Liquid Chromatography-High Resolution/Mass Spectrometry
Kim, So-Hee ; Kim, Ho-Jun ; Son, Jung-Hyun ; Jeon, Byoung-Wook ; Jeong, Eun-Sook ; Cha, Eun-Ju ; Lee, Jae-Ick ;
Mass Spectrometry Letters, volume 3, issue 2, 2012, Pages 50~53
DOI : 10.5478/MSL.2012.3.2.50
After success of sildenafil for the treatment of erectile dysfunction, a large number of its analogues have been approved from FDA. Recently, the illegal dietary supplements which include sildenafil, vardenafil, tadalafil, or analogues of these drugs as ingredient have been widely distributed. Therefore, the determination of the residue of synthetic phosphodiesterase- 5 (PDE-5) inhibitors in dietary supplements is highly required due to indiscriminate and unintentional overdose caused nausea, chest pains, fainting and irregular heartbeat. In this paper, we report a rapid and sensitive analytical method for the simultaneous determination of nine phosphodiesterase-5 inhibitors by liquid chromatography-high resolution mass spectrometry. The present method was found to be accurate and reproducible with 40
/g of the limit of quantification for the nine PDE-5 inhibitors. The developed method can be successfully applied to the analysis of the seven illegal dietary supplements.
Isotope Measurement of Uranium at Ultratrace Levels Using Multicollector Inductively Coupled Plasma Mass Spectrometry
Oh, Seong-Y. ; Lee, Seon-A. ; Park, Jong-Ho ; Lee, Myung-Ho ; Song, Kyu-Seok ;
Mass Spectrometry Letters, volume 3, issue 2, 2012, Pages 54~57
DOI : 10.5478/MSL.2012.3.2.54
Mass spectrometric analysis was carried out using multicollector inductively coupled plasma mass spectrometry (MC-ICP-MS) for the precise and accurate determination of the isotope ratios of ultratrace levels of uranium dissolved in 3%
. We used the certified reference material (CRM) 112-A at a trace level of 100 pg/mL for the uranium isotopic measurement. Multiple collectors were utilized for the simultaneous measurement of uranium isotopes to reduce the signal uncertainty due to variations in the ion beam intensity over time. Mass bias correction was applied to the measured U isotopes to improve the precision and accuracy. Furthermore, elemental standard solution with certified values of platinum, iridium, gold, and thallium dissolved in 3%
were analyzed to investigate the formation rates of the polyatomic ions of
for the concentration range of 50-400 pg/mL. Those polyatomic ions have mass-to-charge ratios in the 230-245 m/z region that it would contribute to the increase of background intensity of uranium, thorium, plutonium, and americium isotopes. The effect of the polyatomic ion interference on uranium isotope measurement has been estimated.
Determination of Mercury in Fly Ash by Using Flow Injection Cold Vapor Isotope Dilution Inductively Coupled Plasma Mass Spectrometry
Suh, Jung-Ki ; Min, Hyung-Sik ; Kamruzzaman, Mohammad ; Lee, Sang-Hak ;
Mass Spectrometry Letters, volume 3, issue 2, 2012, Pages 58~61
DOI : 10.5478/MSL.2012.3.2.58
A method based on flow injection-isotope dilution-cold vapor-inductively coupled plasma mass spectrometry (FI-IDCV-ICP/MS) has been applied to determine trace level of mercury in fly ash.
isotopic spike was added to 0.25 g of BCR176R fly ash and then decomposed by microwave digestion procedure with acid mixture A (8 mL
+ 2 mL HCl + 2 mL HF) and acid mixture B (8 mL
+ 2 mL
+ 2 mL HF) for applying IDMS. Mercury cold vapor was generated by using reductant solution of 0.2% (w/w)
and 0.05% (w/w) NaOH. The measurements of n(
) isotope ratio was made using a quadrupole ICP/MS system. The accuracy in this method was verified by the analysis of certified reference material (CRM) of fly ash (BCR 176R). The indicative value of Hg in BCR 176R fly ash was
mg/kg (k = 2). The determined values of Hg in BCR 176R fly ash by the method of FI-CV-ID-ICP/MS described in this paper were
mg/kg (k = 3.18) and the analysis results were in well agreement with the indicative value within the range of uncertainty.