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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Oriental Pharmacy and Experimental Medicine
Journal Basic Information
Journal DOI :
Kyung Hee Oriental Medicine Research Center, Kyung Hee University
Editor in Chief :
Volume & Issues
Volume 3, Issue 4 - Dec 2003
Volume 3, Issue 3 - Sep 2003
Volume 3, Issue 2 - May 2003
Volume 3, Issue 1 - Feb 2003
Selecting the target year
Resibufogenin induces cardiac arrhythmia
Xie, Jing-Tian ; Mehandale, Sangeeta R. ; Malechar, Spring A. ; Yuan, Chun-Su ;
Oriental Pharmacy and Experimental Medicine, volume 3, issue 2, 2003, Pages 51~55
DOI : 10.3742/OPEM.2003.3.2.051
Resibufogenin is a single compound isolated from the skin venom gland of the toad (Bufo bufo gargarizans cantor). Formulations containing toad venom have been widely used as complementary and alternative medicines. However, like digitalis, resibufogenin possesses both pharmacological and toxicological activities. Our previous data indicated that resibufogenin induces electro-toxicity, including delayed afterdepolarization and triggered arrhythmias at high concentration, both in cardiac fiber in vitro and in beating heart in vivo.
Effect of Terminalia chebula fruit on anaphylaxis by anal therapy
Shin, Hye-Young ; Lee, Kyung-Bo ; Jung, Yun-Hee ; Kim, Eun-Ah ; Lee, Mi-Young ; Lee, Mi-Ri ; Kim, Sang-Yong ; Kim, Sang-Hyun ; Shin, Tae-Yong ;
Oriental Pharmacy and Experimental Medicine, volume 3, issue 2, 2003, Pages 56~62
DOI : 10.3742/OPEM.2003.3.2.056
The effect of aqueous extract of Terminalia chebula fruit (Combretaceae) (TCAE) by anal administration on mast cell-dependent immediate-type anaphylactic reactions was investigated. TCAE (0.005 to 1 g/kg) inhibited systemic anaphylaxis induced by compound 48/80 in mice. When TCAE was pretreated at the same concentrations with systemic anaphylaxis, the plasma histamine levels were reduced in a dose-dependent manner. TCAE (0.1 and 1 g/kg) also significantly inhibited local anaphylaxis activated by anti-DNP IgE. TCAE (0.001 to 1 mg/ml) dose-dependently inhibited the histamine release from rat peritoneal mast cells (RPMC) activated by compound 48/80 or anti-DNP IgE. Moreover, TCAE (0.01 and 0.1 mg/ml) had a significant inhibitory effect on anti-DNP IgE-mediated tumor necrosis
production from RPMC. These results provide evidence that anal therapy of TCAE may be beneficial in the treatment of systemic and local mast cell-dependent anaphylaxis.
A psycho-pharmacological study of BRHAT VATACINTAMANI RASA classical ayurvedic drug
Islam, M. Naimul ; Sattar, Mafruhi ; Haque, Sabera ; Shahriar, Masum ; Choudhuri, Msk ;
Oriental Pharmacy and Experimental Medicine, volume 3, issue 2, 2003, Pages 63~71
DOI : 10.3742/OPEM.2003.3.2.063
The psycho-pharmacological effect of BRHAT VATACHINTAMANI RASA (BVC) an Ayurvedic preparation was investigated, both in animal and clinical models. It was observed that BVC possess a sedative or quitening effect in that it significantly decreased the spontaneous motor activity, and also lowered the exploratory behavior of the amphetamine treated animals. This was further evident by increase in climbing out time and taming effect on animal in isolation induced aggression test. Apart from very high dose it seems have little effect on pentobarbital sleeping time and narcotic analgesic test. The drug BVC increases performance of the animal in forced locomotor test. The effect of VATACHINTAMANI RASA on clinical study was not significant.
Immunomodulatory effect of Tinospora cordifolia in tumor-bearing host
Singh, Nisha ; Singh, Sukh Mahendra ; Shrivastava, Pratima ;
Oriental Pharmacy and Experimental Medicine, volume 3, issue 2, 2003, Pages 72~79
DOI : 10.3742/OPEM.2003.3.2.072
The present investigation was undertaken to study whether tumor-associated macrophages of Daltons lymphoma (DL), a spontaneous transplantable T cell lymphoma can be activated to tumoricidal state by alcoholic extract of Tinospora cordifolia (ALTC). In vivo administration of ALTC (200 mg/kg body weight) in DL-bearing mice resulted in an enhanced RNI production and an augmented cytotoxic response of tumor-associated macrophages. Earlier we had reported that DL-bearing mice show a regression of thymus and an enlargement of spleen. In vivo administration of ALTC to DL-bearing hosts resulted in a decrease in the weight of spleen and counts of splenocytes along with an increase in the weight of thymus as compared to control DL-bearing mice. In vivo administration of ALTC in DL-bearing mice also resulted in an increase in the proliferation of splenocytes/thymocytes and BMC. The results of this study indicate that the ALTC upon in vivo administration in DL-bearing shows immuno-modulatory effects and thus may have clinical significance.
Inhibitory effects of ethanol extract of Atractylodis Rhizoma on melanogenesis in B16/F10 melanoma cells
Chun, Hyun-Ja ; Lee, Jeong-Ho ; Choi, Eun-Young ; Baek, Seung-Hwa ;
Oriental Pharmacy and Experimental Medicine, volume 3, issue 2, 2003, Pages 80~83
DOI : 10.3742/OPEM.2003.3.2.080
The inhibitory effect of Atractylodis Rhizoma alba extract(AM) on melanogenesis was studied by using B16/F10 melanoma in culture. Cells were cultured in the presence of various concentrations of AM for 48 hrs, and the experiment of total melanin content as a final product and activity of tyrosinase, a key enzyme, in melanogenesis. AM significantly inhibited tyrosinase activity, and melanin content in a dose-dependent manner. These results show that Atractylodes macrocephala extract could be developed as skin whitening components of cosmetics.
Antidiabetic effect of Enicostemma littorale Blume aqueous extract in newly diagnosed non-insulin-dependent diabetes mellitus patients (NIDDM): A preliminary investigation
Vasu, Vihas T. ; Ashwinikumar, C. ; Maroo, Jyoti ; Gupta, Sharad ; Gupta, Sarita ;
Oriental Pharmacy and Experimental Medicine, volume 3, issue 2, 2003, Pages 84~89
DOI : 10.3742/OPEM.2003.3.2.084
The antidiabetic efficacy of Enicostemma littorale Blume (chhota chirayata) aqueous extract was examined in newly diagnosed non-insulin-dependent diabetes mellitus (NIDDM) patients taking only the extract and was administered as two divided doses, half an hour before meal as 5g of aqueous extract per single dose. Out of the 20 patients volunteered, 11 successfully completed the 2 month trial and a significant decrease in fasting & postprandial blood glucose and glycosylated haemoglobin levels were observed along with a significant improvement in the antioxidant parameters of the patients. There was also a significant increase in serum insulin levels in 7 patients after extract treatment as compared to levels before treatment. Serum total cholesterol and serum triglyceride levels were decreased significantly with a significant increase in serum HDLCholesterol levels. Other vital parameters remained stable and no side effects were observed. This is the first report showing the hypoglycemic, antioxidant and hypolipidemic properties of the aqueous extract of E. littorale Blume in non-insulin dependent diabetes mellitus patients.
Extracts from Polypodium ferns upregulate the expression of CD95 in human peripheral blood lymphocytes
Lombardi, Valter R.M. ; Etcheverria, I. ; Fernandez-Novoa, L. ; Blanco, A. ; Diaz, J. ; Cacabelos, R. ;
Oriental Pharmacy and Experimental Medicine, volume 3, issue 2, 2003, Pages 90~99
DOI : 10.3742/OPEM.2003.3.2.090
There are several data in the literature indicating a great variety of pharmacological activities of Polypodium genus, which exhibit antiinflammatory and immunomodulatory activities. Since one of our main interests is to obtain natural immunoregulatory agents devoid of pharmacological adverse effects, we used flow cytometry analysis to highlight relative contributions of a water-soluble fraction of different concentrations of Polypodium rhizome extracts on lymphocyte subpopulations, NK and LAK activity. To measure their potential immunoregulatory activity a T cell proliferation assay in response to phytohemaglutinin (PHA) and mixed lymphocyte reactions were chosen. As a confirmatory bioassay we studied the effect of our extracts on CD45RO and CD95 antigen expressions. The results indicate that CD95 expression dramatically increases after peripheral blood lymphocyte activation and treatment with Polypodium leucotomus, cambricum and vulgare extracts, suggesting a powerful intrinsic pro-apoptotic effect.
Multidrug resistance reversal in mouse lymphoma cells by indian tea leaves, indian coffee seeds and chicory
Rao, Bhattiproulu Kesava ; Motohashi, Noboru ; Kawase, Masami ; Spengler, Gabriella ; Molnar, Joseph ;
Oriental Pharmacy and Experimental Medicine, volume 3, issue 2, 2003, Pages 100~105
DOI : 10.3742/OPEM.2003.3.2.100
Systematic analysis of caffeine from the commercial samples of Indian tea leaves was performed by a routine method and the content of caffeine was found to be 19.0-37.4 mg/100 g leaves. The caffeine contents from coffee seeds and chicory from Indian origin were analyzed and found to be 0.6540-1.4920 g/100 g seeds. Caffeine contents of roasted Indian chicory roots were lower than either those of Indian tea leaves or Indian coffee seeds. The multidrug resistance (MDR) reversing effects were tested on a mouse leukemia cell line of L-5178 cells by methanol extracts [M1-M15] of Indian tea leaves and coffee seeds, comparing to a control of
. The effects were measured by fluorescence ratio between treated and untreated group cells. Among fifteen methanol extracts, a Gemini tea [M6] (fluorescence activity ratio 5.26) had the most potent effect for L-5178 cells. The extract M6 was 0.63-fold of
. We suggest that one of mechanisms of reversal by M6 might have strong affinity to dopamine
receptors. Further studies with many more tumor and normal cell lines are necessary to confirm the MDR reversal specificity of coffee methanol extracts.
Antiviral activity of Herba Patrinea (a Chinese medicinal herb) against respiratory syncytial virus in vitro
Li, Hong-Yuan ; Li, Shan-Shan ; Liu, Dian-Li ; Dong, Yan-Mei ; Tian, Wen-Jing ;
Oriental Pharmacy and Experimental Medicine, volume 3, issue 2, 2003, Pages 106~110
DOI : 10.3742/OPEM.2003.3.2.106
Respiratory syncytial virus (RSV) has long been considered an important cause of severe lower respiratory tract infection in infants and young children throughout the world. Unfortunately, no effective treatment of RSV exists. Therefore, New agents are needed to reduce the impact of RSV. We have studied the anti-viral effect of traditional Chinese midicinal herbs for over ten years and find Herba Patrinea (a Chinese medicinal herb) has the anti-RSV effect in vitro. In this study, the Herba Patrinea was extracted with hot water, condensed and sterilized. The cytotoxicity of the aqueous extract was tested by adding the diluted extract directly to HeLa cells and its effect on anti-RSV was estimated by the CPEI assay. As a result, the median cytotoxic concentration
of Herba Patrinea was 32 mg/ ml by morphological observation, the median effective concentration (50% effective concentration,
) of the Herba Patrinea against replication of the Long strain of RSV in HeLa cells were 1.25 mg/ml. The selectivity index
is 25.6. Moreover, Herba Patrinea gave a dose-dependent response in inhibiting RSV. In time of addition experiment, Herba Patrinea inhibited replication of RSV in HeLa cells when it was added at 0h, 2h, and 4h after virus infection. In summary, the results of this study suggest Herba Patrinea may be a novel anti-RSV drug and it is worthy of further studying.