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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Oriental Pharmacy and Experimental Medicine
Journal Basic Information
Journal DOI :
Kyung Hee Oriental Medicine Research Center, Kyung Hee University
Editor in Chief :
Volume & Issues
Volume 5, Issue 4 - Dec 2005
Volume 5, Issue 3 - Sep 2005
Volume 5, Issue 2 - Jun 2005
Volume 5, Issue 1 - Mar 2005
Selecting the target year
A novel therapeutic approach of Hachimi-jio-gan to diabetes and its complications
Yokozawa, Takako ; Yamabe, Noriko ; Cho, Eun-Ju ;
Oriental Pharmacy and Experimental Medicine, volume 5, issue 2, 2005, Pages 75~91
DOI : 10.3742/OPEM.2005.5.2.075
Great efforts have been made to improve both the quality of life and life expectancy of diabetes by treating problems associated with chronic complications such as neuropathy, retinopathy and nephropathy. In particular, diabetes is an increased risk of developing several types of kidney disease, and the predominant cause of end-stage renal disease in patients with this disorder is diabetic nephropathy. Therefore, prevention of the occurrence and progression of diabetes and its complications has become a very important issue. The scientific observations of an animal model of streptozotocin-induced diabetes, spontaneously occurring diabetes and diabetic nephropathy in this study suggest that one of the Kampo prescriptions, Hachimi-jio-gan comprising eight constituents, is a novel therapeutic agent.
Antioxidant potential of aerial part of Asclepias curassavica. Linn (Family-Asclepiadaceae)
Raja, S ; Ahamed, KFH Nazeer ; Kumar, V ; Mukherjee, Kakali ; Bandyopadhyay, Arun ; Mukherjee, Pulok K ;
Oriental Pharmacy and Experimental Medicine, volume 5, issue 2, 2005, Pages 92~99
DOI : 10.3742/OPEM.2005.5.2.092
Asclepias curassavica. Linn, an erect, simple (or) much branched perennial herb with a somewhat woody base, belonging to the family Asclepiadaceae. It has been reported to have multiple pharmacological effect of anti-inflammatory, cardiotonic, anticancer, anthelmintic and to treat piles and gonorrhoea. It is to be expected that several activities might be related to a possible antioxidant action from this plant. The hydro alcoholic extract of Asclepias curassavica was tested in vitro for its antioxidant activities, such as DPPH radical, nitric oxide radical, superoxide anion radical, lipid peroxidation assay, hydroxyl radical, reducing power, and total phenol content. The extract exhibited scavenging potential with
for DPPH, nitric oxide and superoxide anion radicals. The values were found to higher than those of Vitamin-C, rutin, and curcumin, as standards. The extract showed 50% protection at the dose of
in lipid peroxidation as well as deoxyribose degradation, those values more to that of standard, vitamin E
. The reducing power of the extract depends on the concentration and amount of extract. Since a significant amount of polyphenol could be detected by the equivalent to
of pyrocatechol from 1 mg of extract. It can be concluded that hydro alcoholic extract of aerial part of Asclepias curassavica could be considered as potent antioxidant, which makes it suitable for the prevention of human disease.
Antioxidant activity and anti-tumor immunity by Propolis in mice
Choi, In-Sook ; Itokawa, Yuka ; Maenaka, Toshihiro ; Yamashita, Takenori ; Mitsumoto, Morihide ; Tano, Kaoru ; Kondo, Hiroyo ; Ishida, Torao ; Nakamura, Takashi ; Saito, Kiyoto ; Terai, Kaoru ; Monzen, Hajime ; Oshima, Masami ; Takeuchi, Tetsuo ; Mituhana, Yuicti ; Bamen, Kenichi ; Ahn, Kyoo-Seok ; Gu, Yeun-Hwa ;
Oriental Pharmacy and Experimental Medicine, volume 5, issue 2, 2005, Pages 100~109
DOI : 10.3742/OPEM.2005.5.2.100
In South America, natural products with unknown drug effects are used as folk remedies and for preventive medicine. Among South American natural products, we directed our attention to Propolis, which have been known as medicinal plants, and examined the mechanisms by which these substances affect antioxidant activity, anti-tumor activity and immunoresponse. When the antioxidant activities of Propolis were examined by the DPPH and Rhoudan iron methods, since Propolis contains high levels of flavonoids, it is thought that flavonoids may be responsible for the antioxidant activity in this study. In the examination of immunoenhancement activity, we measured lymphocyte versus polymorphonuclear leukocyte ratios (L/P activity). The number of lymphocytes was significantly increased in groups treated with Proplolis. Specifically, slightly high levels of
were measured in mice bearing the S-180 carcinoma, after administration of Propolis. This strongly suggests that cellular immunity is especially activated by treatment with Propolis, because production of
is limited to the T cells and NK cells stimulated by mitogen and sensitized antigen.
shows a different extent and mechanism of action depending on the target cells. When
was measured in mice bearing the S-180 carcinoma, mice treated with Propolis showed slightly higher
levels as compared to the control group. This suggests that activated macrophages produce
in mice treated with Prapolis, since activated macrophages and lymphocytes are the source of most
. When anti-tumor action was examined using two kinds of sarcoma (Ehrlich solid carcinoma and Sarcoma-180 carcinoma), tumor-suppressive ratios after treatment with Propolis was 29.1%. When Sarcoma-180 solid carcinoma was used, tumor-suppressive ratios were 62%. Thus, Propolis showed strong anti-tumor activity against two kinds of solid carcinoma. Taken altogether, this strongly suggests that Propolis enhances original functions of macrophages and NK cells, and as a result, secondarily enhances the immune reaction and suppresses tumor growth.
In vitro antifilarial potential of the leaves and stem extract of Pluchea lanceolata on the cattle filarial parasite Setaria cervi
Mishra, Vandna ; Parveen, Nazneen ; Khan, Nizam U. ; Singhal, KC ;
Oriental Pharmacy and Experimental Medicine, volume 5, issue 2, 2005, Pages 110~116
DOI : 10.3742/OPEM.2005.5.2.110
The effect of alcohol and aqueous extracts of the leaves and stem of Pluchea lanceolata on the spontaneous movements of both the whole worm and the nerve muscle preparation of Setaria cervi, and on the survival of microfilariae in vitro was studied. Alcohol and aqueous extracts of the leaves and stem of P. lanceolata caused the inhibition of spontaneous movements of the whole worm and the nerve muscle preparation of S. cervi, characterized by short lasting small increase in tone and amplitude of contractions followed by paralysis. The concentrations required to inhibit the movements of whole worm and nerve muscle preparations for alcohol extract were 200 and
, and for aqueous extract were 250 and
, respectively, suggesting a cuticular permeability barrier. Both the extracts (alcohol and aqueous) caused death of microfilariae in vitro,
being 12 and 18 ng/ml for alcohol extract and 25 and 40 ng/ml for aqueous extract, respectively.
Species identification of Dyers woad leaf by DNA sequence of 5S-rRNA spacer domain and random amplified polymorphic DNA (RAPD) analysis
Zhao, K.J. ; Dong, T.T.X. ; Wong, Y.C. ; Tu, P.F. ; Tsim, K.W.K. ;
Oriental Pharmacy and Experimental Medicine, volume 5, issue 2, 2005, Pages 117~123
DOI : 10.3742/OPEM.2005.5.2.117
Dyers woad leaf (Daqingye) is a traditional Chinese medicine commonly used as anti-pyretic, anti-bacterial and anti-viral agent against infectious diseases. The Chinese Pharmacopoeia (2005) records that Dyers woad leaf should be derived from the leaves of Isatis indigotica Fort., but the leaves of Polygonum tinctorium Ait., Baphicacanthus cusia (Nees) Bremek. and Clerodendron cyrtophyllum Turcz. have also been used as substitutes of Dyers woad leaf in different regions of China. The leaf morphologies of these four species show a close resemblance, and based on their morphological appearance, it is difficult to identify them. Here, molecular genetic methods were developed as a target to identify different members of Dyers woad leaf. The 5S-rRNA spacer domain was amplified by polymerase chain reaction from genomic DNAs isolated from I. indigotica, P. tinctorium, B. cusia and C. cyrtophyllum, and the nucleotide sequences showed a great diversity. In addition, random amplification of polymorphic DNA analysis was also used to distinguish the members of Dyers woad leaf. These molecular methods could be used as a tool in authentic identification of Dyers woad leaf.
Analgesic and antiinflammatory activity of Alstonia macrophylla and Mallotus peltatus leaf extracts: Two popular ethnomedicines of Onge, a Negrito tribe of little Andaman
Chattopadhyay, Debprasad ; Arunachalam, G. ; Sur, T.K. ; Bhattacharya, S.K. ; Mandal, Asit B. ;
Oriental Pharmacy and Experimental Medicine, volume 5, issue 2, 2005, Pages 124~136
DOI : 10.3742/OPEM.2005.5.2.124
Two popular ethnomedicines of the Onge, a Negrito tribe of Andaman Islands, were evaluated for analgesic and antiinflammatory activity. The methanol extract as well as the different fractions of methanol extract of both Alstonia macrophylla and Mallotus peltatus leaves were studied using Swiss albino mice and Wistar albino rats. Acetic acid induced writhing, Tail flick and Tail immersion; Carrageenin- and Dextran-induced paw oedema tests were used. Dose-dependent analgesic and antiinflammatory activity were demonstrated for both methanol leaf extracts as well as fractions. Results were highly comparable with that of the standard drug pethidine.
Protective effect of green tea extract on doxorubicin induced cardiotoxicity in rats
Patil, Leena ; Balaraman, R. ;
Oriental Pharmacy and Experimental Medicine, volume 5, issue 2, 2005, Pages 137~143
DOI : 10.3742/OPEM.2005.5.2.137
Doxorubicin induces oxidative stress leading to cardiotoxicity causing electrocardiogram abnormalities and increases in biomarkers associated with toxicity. Green tea extract (GTE) is reported to possess antioxidant activity mainly via its polyphenolic constituent, catechins. This study was intended to determine the effect of various doses of GTE (25, 50 and 100 mg/kg/day p.o. for 30 days) on doxorubicin-induced electrocardiographic and biochemical changes in rat heart. The latter included lactate dehydrogenase, creatine kinase, and glutamic oxaloacetate transaminase in serum and superoxide dismutase, catalase, and reduced glutathione, as well as membrane bound enzymes like
and decreased lipid peroxidation in heart tissue Results demonstrated that rats which received GTE were less susceptible to such changes indicating protection afforded by GTE.
Effects of QZ-16 on blood glucose and lipids in Streptozotocin induced diabetic rats
Najmi, Abul K. ; Pillai, K.K. ; Ahmad, Aftab ; Aqil, M. ;
Oriental Pharmacy and Experimental Medicine, volume 5, issue 2, 2005, Pages 144~149
DOI : 10.3742/OPEM.2005.5.2.144
The present study was designed to investigate the hypoglycaemic and hypolipidaemic activities of Qurs-e-Ziabetus 16 (QZ-16) in Streptozotocin (STZ) induced diabetic rats. QZ-16, a polypharmaceutical herbomineral formulation developed on the principles of Unani medicine is used for non-insulin dependent diabetes mellitus (NIDDM). The elevated levels of fasting blood glucose, serum levels of cholesterol, triglycerides and urea observed in rats treated with STZ (55 mg/kg body wt.) were significantly reduced by the treatment of QZ-16 (240 mg/kg, p.o.) and gliclazide (30 mg/kg, p.o.). The reduced HDL cholesterol levels were also increased by the QZ-16 and gliclazide treatments in the STZ induced diabetic rats. These data show that QZ-16 has hypoglycaemic, hypolipidaemic properties in STZ induced diabetic rats.
Effect of antidiarrhoeal formulation on acute experimental diarrhoea in rats
Khan, M. Aleem ; Khan, Naeem A. ; Qasmi, Iqbal A. ; Ahmad, Ghufran ; Zafar, Shadab ;
Oriental Pharmacy and Experimental Medicine, volume 5, issue 2, 2005, Pages 150~155
DOI : 10.3742/OPEM.2005.5.2.150
Aqueous extract of antidiarrhoeal formulation (ADF) containing Holarrhena antidysentrica, Aegle marmelos and Punica granatum was investigated for antidiarrhoeal activity against charcoal-induced gut transit, serotonin-induced diarrhoea and
small intestine enteropooling in rats. The control, standard and test groups of experimental animals were administered with normal saline (p.o.), diphenoxylate hydrochloride (5 mg/kg, p.o.) and ADF (250 mg and 500 mg/kg, p.o.) respectively except the control group of
small intestine enteropooling which received only 5% ethanol in normal saline (i.p.). Charcoal (10 ml/kg, p.o.) and serotonin
were administered after 30 min, while
was administered immediately afterwards. The distance traveled by charcoal in small intestine was measured after 15 and 30 min of charcoal administration, diarrhoea was observed every 30 min for six hour after serotonin administration and the volume of intestinal fluid was measured after 30 min of
administration. Oral administration of ADF significantly inhibited the frequency of defaecation and decreased the propulsion of charcoal meal through the gastrointestinal tract, reduced the wetness of faecal dropping in serotonin-induced diarrhoea and also reduced the
small intestine enteropooling. ADF may have potential to reduce the diarrhoea in rats.
Experimental research for the protective effect of Naoxingtong-containing serum on rat cerebral microvascular endothelial cells
Jun, Zhou ; Jianyou, Guo ; Jian, Guo ; Lanfang, Li ; Canghai, Li ; Nan, Jiang ; Shuying, Guo ; Hairu, Huo ; JiangTingliang, JiangTingliang ;
Oriental Pharmacy and Experimental Medicine, volume 5, issue 2, 2005, Pages 156~159
DOI : 10.3742/OPEM.2005.5.2.156
The protective effect of Naoxingtong (NXT) on rat cerebral microvascular endothelial cell (rCMEC) was investigated. rCMEC was injured in vitro by incubating for 4 hours at 100% NO in a hypoxia chamber. After treated with NXT-containing serum, the cellular viability rate (90.3%) was significantly elevated when compared with that of control group and the inhibitive rate of lactic dehydrogenase activity (9.2%) was far lower than the control group with dose-dependent effect. The results indicate that NXT can increase viability of rCMEC, and protect cell membrane from injury during hypoxia.
Hypoglycemic effect of a polyherbal aqueous extract in experimentally induced diabetic rats
Vasu, Vihas T. ; Thaikoottathil, Jyoti V. ; Gupta, Sarita ;
Oriental Pharmacy and Experimental Medicine, volume 5, issue 2, 2005, Pages 160~166
DOI : 10.3742/OPEM.2005.5.2.160
The present study was carried out to investigate the hypoglycemic effect of a polyherbal aqueous extract (Curcuma longa Linn., Emblica officinalis Gaertn., Trigonella foenum-graecum Linn., Enicostemma littorale Blume) in alloxan-induced diabetic rats. Short term experiments showed a decrease in blood glucose levels at
of administration of the aqueous extract in alloxan-induced diabetic rats with increase in serum insulin levels. The extract did not show any effect on blood glucose or serum insulin levels in normoglycaemic rats. Treatment with the extract (1.5 g dry plant equivalent extarct/100 g body weight/day) for 20 days in diabetic rats showed a significant decrease in blood glucose and glycosylated haemoglobin levels and an increase in serum insulin levels. The aqueous extract also showed an enhanced glucose-induced insulin release at 11.1 mM glucose from isolated rat pancreatic islets. The extract did not show any toxicity at the particular dose used.
Effect of Sho-Seiryu-To (XIAO-QING-LONG-TANG) on skin itching and peripheral eosinophil level in three elderly patients
Murata, Kohji ; Toriumi, Yoshitaka ; Kamei, Tsutomu ;
Oriental Pharmacy and Experimental Medicine, volume 5, issue 2, 2005, Pages 167~171
DOI : 10.3742/OPEM.2005.5.2.167
Sho-Seiryu-To is known to be effective against allergic diseases, but its effect on skin itching has not been reported. We observed the effect of Sho-Seiryu-To on three elderly patients who, desoite using an anti-allergic drug, has severe chronic skin itching with peripheral eosinophilia. All three patients decreased their peripheral eosinophilia and improved their skin condition within eight weeks. We conclude that Sho-Seiryu-To could be capable of treating elderly patients with chronic skin itching and peripheral eosinophilia.