Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Oriental Pharmacy and Experimental Medicine
Journal Basic Information
Journal DOI :
Kyung Hee Oriental Medicine Research Center, Kyung Hee University
Editor in Chief :
Volume & Issues
Volume 6, Issue 4 - Dec 2006
Volume 6, Issue 3 - Sep 2006
Volume 6, Issue 2 - Jun 2006
Volume 6, Issue 1 - Mar 2006
Selecting the target year
Commonly used herbal medicines in the treatment for female reproductive dysfunction
Imai, Atsushi ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 1, 2006, Pages 1~11
DOI : 10.3742/OPEM.2006.6.1.001
Herbal medicines are widely used for centuries to treat illness and improve health in Asia and the use of these remedies has become a worldwide form of alternative therapy. However, there is very limited evidence from randomized controlled trials to support the efficacy of the vast majority of herbal products. Of the commonly used herbal medicines, systemic clinical experiences and promising experiments in animals have found potential evidence of efficacy for six herbal medicines (Unkei-to, Hachimijio-gan, Keishi-bukuryo-gan, Toki-shakuyaku-san, Shakuyaku-kanzo-to, Sairei-to) in the treatment for female reproductive dysfunction. Because herbs may contain potent bioactive substances, the physician should have an opportunity to outline more stringent regulation, similar to over-the counter drug.
Damage of radioprotection and antitumor effects of water-soluble propolis
Terai, Kaoru ; Ryu, Myung-Sun ; Itokawa, Yuka ; Maenaka, Toshihiro ; Nakamura, Takashi ; Hasegawa, Takeo ; Choi, In-Suk ; Ishida, Torao ; Gu, Yeun-Hwa ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 1, 2006, Pages 12~20
DOI : 10.3742/OPEM.2006.6.1.012
Some natural products are able to inhibit radiation effects and exert an antitumor effect with fewer adverse reactions; however, their antitumor effects are less than those of widely-used synthetic drugs. Propolis is a natural material that has been attracting attention, and we extracted this material with water and investigated the effect of continuous propolis administration on radioactivity-induced reduction of hemocytes, in addition to the antioxidant and antitumor effects of propolis. Following a 1-week adjustment period, water-soluble propolis was administered intraperitoneally to male ICR mice at a dose of 100 mg/kg every other day for 2 weeks. Following administration, 2 Gy whole-body irradiation was performed and the counts of leukocytes, lymphocytes, and granulocytes and monocytes in the peripheral blood were determined 1, 3, 7, 15 and 30 days after irradiation. These cells were considered since they are closely associated with immunity to radioactivity. In a second experiment, water-soluble propolis was similarly administered to the mice for 2 weeks after a 1-week adjustment period, and 2 Gy whole-body irradiation was performed. The antioxidant effects in hemocytes were then investigated using 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH), a radical generator. In a third experiment,
Sarcoma-180 cells were inoculated into the right thigh of mice, which were divided into four groups: control, water-soluble propolis-treated, 6 Gy irradiated and water-soluble propolis-treated + 6 Gy irradiated groups, and changes in tumor size were measured for 20 days. Statistical analysis was conducted using ANOVA for multiple groups. In the three experiments, administration of water-soluble propolis inhibited the reduction of hemocytes caused by whole-body irradiation, showed antioxidant effects against radioactivity, and inhibited tumor growth, respectively. In conclusion, our data suggest that the antioxidant effect of watersoluble propolis inhibits hemocyte reduction caused by whole-body irradiation and enhances immunological inhibition of tumor growth.
Central nervous system stimulating activity of the ethanolic extract of Fleurya interrupta Guad. (Urticaceae)
Shilpi, Jamil Ahmad ; Rouf, Razina ; Ferdous, MM ; Uddin, Shaikh Jamal ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 1, 2006, Pages 21~26
DOI : 10.3742/OPEM.2006.6.1.021
The ethanolic extract of Fleurya interrupta Gaud, (Urticaceae) was tested for its possible neuropharmacological effects on experimental animals, For the primary neuropharmacological screening of this plant, the ethanolic extract of its aerial parts was subjected to preliminary evaluation for acute toxicity, antinociceptive activity and central nervous system (CNS) activities. At the doses of 125 and 250 mg/kg, the extract significantly (P < 0.01 and P < 0. 001) and dose-dependently increased the frequency of acetic acid induced writhing in mice. In the pentobarbitone induced sleeping time test, the extract at the above dose levels, significantly and dose-dependently decreased the pentobarbitone induced sleeping time (P < 0.001) and increased the time for onset of sleep (P < 0.001) in mice. In the open field and hole cross tests, test animals showed an increase in their movement in the both tests from the 2nd observation period (30 min) and persisted throughout the entire experimental period (240 min). These results of the extract may attribute a stimulating action on the CNS. On the basis of these findings, it can be assumed that the extract exerts its stimulating effect on the CNS in mice by interfering with the cortical function or increasing the effect of some CNS stimulating neurotransmitters.
Susceptibility for ischemic stroke in Sasang constitutional classification is associated with the interleukin-1 receptor antagonist polymorphism
Lee, Byung-Cheol ; Ahn, Young-Min ; Ahn, Se-Young ; Doo, Ho-Kyung ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 1, 2006, Pages 27~33
DOI : 10.3742/OPEM.2006.6.1.027
The Sasang constitutional medicine classifies the mankind into four constitutional types according to the individual psychological and physical traits. Differences in the Sasang constitutional medicine may be explained by the genetic factors. In order to determine the association of Sasang constitutional classification and interleukin-1 receptor antagonist (IL-1Ra) in genetic susceptibility to ischemic stroke, we classified the four constitutional types in ischemic stroke patients (n = 125) and the healthy control subjects (n = 107), and genotyped for IL-1Ra polymorphism by polymerase chain reaction (PCR) methods. The distribution of the
genotype in the ischemic stroke patients was significantly different from the healthy controls (OR = 6.09; P =0.0134). And the prevalence of
genotype was increased in Taeum-in ischemic stroke patients, as compared to Taeum-in healthy controls (OR = 14.71; P = 0.0144). These results suggest that
genotype in Taeum-in might be associated with the increasing risk for ischemic stroke. Furthermore, this relationship could provide the basis for a new approach in the investigation of the etiology of ischemic stroke.
Neuropharmacological study of hot water extract of the seeds of Vernonia anthelmintica Kuntze
Rahman, Mahbubur ; Choudhuri, M Shahabuddin Kabir ; Khan, Mahmud Tareq Hassan ; Jabbar, Shaila ; Alamgir, Mahiuddin ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 1, 2006, Pages 34~38
DOI : 10.3742/OPEM.2006.6.1.034
The hot water extract of the seeds of Vernonia anthelmintica Kuntze (Compositae) in a dose of 10 ml/kg body weight of mice, showed significant analgesic activity on the hot plate analgesic method throughout the 4 h experimental period. The hole cross scores and the climbing out scores are of lower than the control animals. The hot water extract reduced the defecation in hole board study and significantly reduces the exploratory ambulation and head dipping behaviour. The seed also showed significant depressant activity on the exploratory ambulation of the open field scores. The drug decreased the spontaneous locomotion activity on brick-chip displacement method up to 45 min and also showed the ability to lessen the amphetamine induced hyperactivity up to 20 min. But it didn't show any effect on pentobarbital induced sleeping time test. The extract reduced gastrointestinal motility.
Effects of the water of yellow soil, Ji-Jang-Soo on cell viability and cytokines production in immune cells
Jeong, Hyun-Ja ; Hwang, Gab-Soo ; Myung, No-Il ; Lee, Joon-Ho ; Lee, Ju-Young ; Um, Jae-Young ; Kim, Hyung-Min ; Hong, Seung-Heon ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 1, 2006, Pages 39~44
DOI : 10.3742/OPEM.2006.6.1.039
Ji-Jang-Soo (JJS) is known to have a detoxification effect. However, it is still unclear how JJS has these effects in experimental models. In this study, we investigated the effect of JJS on the viability of cells and production of cytokines in human T-cell line, MOLT-4 cells, and human mast cell line, HMC-1 cells. The MOLT-4 cells were cultured for 24 h in the presence or absence of JJS. As the result, JJS (1/100 dilution) significantly increased the cell viability about 78% (P < 0.05) and also increased the interleukin (IL)-2, and interferon
production compared with media control at 24 h. But had no effect on IL-4 production. Hypoxia mimic compound, desferroxamine (DFX) decreased the immune cell viability. Cell viability decreased by DFX was increased by JJS. In conclusion, these data indicate that JJS may have an immune-enhancing effect.
Hepatoprotective activity of methanol extracts of Berberis tinctoria
P, Vijayan ; HC, Prashanth ; Vijayaraj, Preethi ; H, Raghu Chandrashekhar ; Godavarthi, Ashok ; SA, Dhanaraj ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 1, 2006, Pages 45~52
DOI : 10.3742/OPEM.2006.6.1.045
The methanol extracts of the roots, root bark and stem of Berberis tinctoria, were investigated for their hepatoprotective activity against carbon tetrachloride
induced toxicity in freshly isolated rat hepatocytes, HEp-G2 cells and animal models. The methanol extracts were able to significantly normalise the levels of aspartate amino transferase, alanine aminotransferase, alkaline phosphatase, triglycerides, total proteins, albumin, total bilirubin and direct bilirubin, which were altered due to
intoxication in freshly isolated rat hepatocytes and also in animal models. The anti-hepatotoxic effect of the methanol extracts in vitro were observed at
concentrations. A dose dependent increase in the percentage viability was observed when
exposed HEp-G2 cells were treated with different concentrations of the methanol extracts. The highest percentage viability of HEp-G2 was observed at a concentration of
. The results from the present investigations also indicate good correlation between the in vivo and in vitro studies.
Protective effect of Indigofera aspalathoides in chemical induced gastric mucosal lesions in rats
Gupta, M ; Mazumder, UK ; Haldar, PK ; Kander, CC ; Manikandan, L ; Senthilkumar, GP ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 1, 2006, Pages 53~57
DOI : 10.3742/OPEM.2006.6.1.053
The plant Indigofera aspalathoides is used by a large number of tribes in India for the treatment of various hepatic disorders and abscesses. The methanol extract of Indigofera aspalathoides (MEIA) was evaluated for its protective effects on gastric mucosal lesion in Wister albino rats against indomethacin, histamine and ethanol induced gastric mucosal damage. The response to MEIA was assessed using the ulcer index, thiobarbituric acid reactive substance (TBARS), and glutahione level. MEIA pretreatment showed protection against chemical induced gastric mucosal damage, a significant reduction in the ulcer index and TBARS activity and increase glutathione level as compared with that of standard drugs.
Free radical scavenging activity of some Mangroves available in Bangladesh
Ahmed, Firoj ; Shahid, IZ ; Razzak, MA ; Rahman, M Mostafizur ; Hoque, Tahmina ; Rahman, MT ; Sadhu, SK ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 1, 2006, Pages 58~64
DOI : 10.3742/OPEM.2006.6.1.058
The crude alcoholic extracts of various parts of five different Bangladeshi mangrove plants (Amoora cucullata, Caesalpinia bonducella, Cerbera odollam, Derris uliginosa and Sonneratia caseolaris) were screened in-vitro for anti-oxidant activity using the 1,1-diphenyl-2-picryldydrazyl-hydrate (DPPH) free radical scavenging assay. Of all of them, the ethanolic extracts of the leaves of Derris uliginosa and Sonneratia caseolaris showed potential antioxidant activity (
: 94.406 and 26.30 mg/ml respectively) whereas the ethanolic extracts of aerial parts of Derris uliginosa, barks of Cerbera odollam and leaves and stems of Caesalpinia bonducella showed moderate activity (
: 125.89, 211.35 and 301.99 mg/ml respectively). Mild anti-oxidant activity was observed with the methanolic extract of leaves of Amoora cucullata and ethanolic extracts of fruits of Cerbera odollam.
Analysis of flavonoids in the mature fruit of Vaccinium uliginosum L. of China
Hua, Lian Li ; Yoshitama, Kunijiro ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 1, 2006, Pages 65~67
DOI : 10.3742/OPEM.2006.6.1.065
In this study, a new extraction method was developed and two kinds of flavonoids were extracted from the mature fruit of Vaccinium uliginosum L. of China. These two kinds of flavonoids were analyzed by spectral and identified by high-performance liquid chromatography (HPLC) and UV/Vis. The extract of the fruit was total acid hydrolyzed. TLC chromatography was subsequently employed to identify the hydrolysate. Two kinds of aglycone flavonoids, quercetin and myricetin, were identified. At the same time PC chromatography was used to identify the monomer sugar in the flavonoids and it was verified as glucose. HPLC, UV/Vis, and Mass spectrum analyses revealed that the flavonoids were quercetin 3-monoglucosides and myricetin 3-monoglucosides.