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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Oriental Pharmacy and Experimental Medicine
Journal Basic Information
Journal DOI :
Kyung Hee Oriental Medicine Research Center, Kyung Hee University
Editor in Chief :
Volume & Issues
Volume 6, Issue 4 - Dec 2006
Volume 6, Issue 3 - Sep 2006
Volume 6, Issue 2 - Jun 2006
Volume 6, Issue 1 - Mar 2006
Selecting the target year
The phyto-oestrogens: its anticarcinogenic and antioxidant activity-a review
Widyarini, Sitarina ; Reeve, Vivienne E. ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 2, 2006, Pages 69~78
DOI : 10.3742/OPEM.2006.6.2.069
The isoflavonoids comprise a group of phyto-oestrogens that have useful biological activities including oestrogenic, antioxidant and anticancer. As dietary components for humans, they are bioavailable from leguminous vegetables (such as genistein from soybean), and have been well-documented to have numerous health benefits. A wide range of epidemiological studies in humans and limited studies in animals have identified isoflavonoids as potential chemopreventive agents against hormone-dependent cancers. Therefore, an attempt has been made through this review to summarise the information in the mechanisms aspect of isoflavonoid phyto-oestrogens in inhibiting cancer in vitro and in vivo in the models of human cancers.
Study of acupuncture stimulation on experimental osteopenia
Kanai, Shigeyuki ; Taniguchi, Norimasa ; Kanda, Kayo ; Higashino, Hideaki ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 2, 2006, Pages 79~85
DOI : 10.3742/OPEM.2006.6.2.079
In order to study the effect of acupuncture stimulation on bone mineral density (BMD), using the ovariectomized (OVX) rat model, we assessed the degree of osteopenia by dual-energy X-ray absorptiometry, measured the level of locomotor activity using a metabolism measuring system, and performed histological studies of bone tissue. Twenty-four female Wistar rats (8 weeks old, 160 - 180 g)were divided into three groups. Rats in the OVX-A group underwent ovariectomy followed by acupuncture stimulation. The OVX rats in the Vehicle control group were not treated with acupuncture as a control. The rats in the control group received neither ovariectomy nor acupuncture. Acupuncture stimulation for 12 weeks in the OVX-A group inhibited the reduction in BMD of the femoral bones caused by ovariectomy. Moreover, in the two OVX groups, there was no clear difference in the level of locomotor activity between the active and resting phases prior to acupuncture stimulation in each rat, and the pattern of locomotor activity was irregular. After acupuncture stimulation of the OVX-A rats, the pattern of locomotor activity became diphasic with clear active and resting phases, as was observed in the Control group. On histological studies, the continuity of trabecular bone was maintained more favorably and bone mass was higher in the OVX-A group than in the vehicle control group. These results suggest that the increased locomotor activity that had been induced by acupuncture stimulation increased the BMD.
Spermatogenic index and hormonal profile in the rats received chromatographic fractions of ethanol extract of Crotalaria juncea L. seeds
Malashetty, Vijaykumar B. ; Patil, Saraswati B. ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 2, 2006, Pages 86~95
DOI : 10.3742/OPEM.2006.6.2.086
The ethanol extract of the Crotalaria juncea seeds, which showed promising antispermatogenic and antiandrogenic activities in albino mice, was taken up further for the isolation of the active fractions present in it. Two fractions that were obtained from thin layer chromatography were subjected for testing to know their antispermatogenic and antiandrogenic activities. After preliminary trials the fraction I showed maximum antifertility activity at the dose level of 200 mg/kg body weight when administered orally to the rats for 50 days. The fraction I was found to affect spermatogenesis as well as the endocrine functions of the testis as indicated by gravimetric, histopathological and biochemical changes. Further this fraction has caused degenerative changes in the seminiferous tubules and Leydig cells of the testis. The accessory reproductive organs like epididymis, seminal vesicles, vas deferens, prostrate, Cowper's gland and Levator Ani muscle showed significant malfunction. Cauda epididymal sperm count and sperm motility were reduced significantly. The treatment has also resulted in increase in the cholesterol level and alkaline phosphatase activity, and decrease in protein, glycogen, sialic acid contents and acid phosphatase activity in testis. It is noteworthy that RIA studies have shown significant reduction in serum FSH, LH and testosterone. Scanning electron microscopic observations revealed abnormalities in sperm structure.
Antinociceptive activity of some Bangladeshi medicinal plant extracts
Uddin, S.J. ; Shilpi, J.A. ; Rouf, R. ; Ferdous, M.M. ; Nahar, L. ; Sarker, S.D. ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 2, 2006, Pages 96~101
DOI : 10.3742/OPEM.2006.6.2.096
The extracts of some Bangladeshi medicinal plants, Possur (Xylocarpus mekongensis), Dhundul (Xylocarpus granatum), Gab (Diospyros peregrina), Kadom (Anthocephalus chinensis) and Sundari (Heritiera fomes), were assessed for their possible antinociceptive activity using acetic acid induced writhing model in mice. Most of these plants have been used in traditional medicine in Bangladesh as well as in other countries for the treatment of various ailments ranging from common cold to cancer. All these extracts significantly inhibited the acetic acid induced writhing in mice at the oral dose of 500 mg/kg body weight. The extract of Anthocephalus chinensis bark showed the most potent writhing inhibition (69.47%, P < 0.001) and that of Diospyros peregrina bark had the least (33.54%, P< 0.02).
Bioactivity of the methanol extract of Excoecaria agallocha Linn.(Euphorbiaceae)
Rajia, S. ; Alamgir, M. ; Shahriar, M. ; Choudhuri, M.S.K. ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 2, 2006, Pages 102~107
DOI : 10.3742/OPEM.2006.6.2.102
The methanol extract and residual methanol fraction of Excoecaria agallocha L. (Euphorbiaceae) stem bark was investigated in this study by wheat rootlet and shoot growth inhibition, and antimicrobial bioassay. The methanol extract and residual methanol fraction showed high inhibitory effect on both the wheat rootlet (82-89%) and shoot growth (85-90%) compared to control. The methanol extract showed a better and dose related inhibition on both the rootlet and shoot growth compared to residual methanol fraction. The
value of methanol extract for rootlet and shoot were
, and of residual methanol fraction for rootlet and shoot were
. The methanol extract and residual methanol fraction did not show any antibacterial activity against the tested microorganisms of clinical isolates Escherichia coli, Staphylococcus aureous, Pseudomonas aeruginosa, Proteus vulgaris and Bacillus subtilis. The plant has the potential to be a source of novel cytotoxic compound(s).
Protective effect of Kundur (Boswellia serrata) against mercuric chloride induced nephrotoxicity in rats
Alam, M. ; Javed, K. ; Jafri, M.A. ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 2, 2006, Pages 108~111
DOI : 10.3742/OPEM.2006.6.2.108
The effect of Kundur (oleo-gum resin of Boswellia serrata) and its fractions viz: methanol soluble (MS) and methanol insoluble (MI) were investigated on mercuric chloride induced nephrotoxicity in rats. The animals of group I and II were administered with 1% carboxymethyl cellulose (CMC) (1,000 mg/kg, p.o.) and the animals of groups III, IV and V were administered with Kundur (1,000 mg/kg, p.o.), MS (650 mg/kg, p.o.) and MI (350 mg/kg, p.o.) respectively for ten days. On 10th day a single dose of the mercuric chloride (3 mg/kg, 5.c.) was also administered to all groups except the group I which received only 1% CMC (10 ml/kg, p.o.). After two days of mercuric chloride administration the blood samples of each animal were collected and analyzed for blood urea nitrogen and creatinine concentration. Rats fed with Kundur and MI fraction showed a significant prevention in the rise of serum markers while MS failed to prevent the rise of these serum makers. These results suggest that Kundur and MI fraction may have potential to reduce the nephrotoxicity in rats.
Objective evaluation of the color of tongue substance using L
Park, Young-Jae ; Park, Young-Bae ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 2, 2006, Pages 112~120
DOI : 10.3742/OPEM.2006.6.2.112
The purpose of this study was to analyze whether quantitative evaluation of the color of the tongue substance using
color coordinates system could minimize the problems arising from the different illuminating conditions or not. In controlled 4 different illuminating conditions (by natural light, flashlight, f-number, shutter speed),12 healthy subjects were photographed of their tongue substance through a digital camera (C-2100uz, Olympus Co.), both on the top surface and on the bottom surface of the tongue substance by two examiners, twice at 3 day intervals. Clinician evaluation was also performed grading the redness of the tongue substance in the form of 5-points scale by 6 clinicians. As a result, there was no significant difference in color differences between the color of the tongue substance and the reference red card in the 4 different illuminating conditions. Intra-rater reliability was satisfied and even though limitedly, inter-rater reliability was satisfied. Color differences were significantly correlated with the results by the clinicians, although they were applicable limitedly to specific illuminating conditions. Our results indicate that the application of the color differences in tongue diagnosis could not only evaluate the color information quantitatively, but also minimize the problems arising from the different illuminating conditions and that there was the significant difference in the visual evaluation of the red color of the tongue substance, both between the clinicians and between the illuminating conditions.
Analgesic, cytotoxic and antioxidant activities of Trewia polycarpa bark
Rahman, Md Shafiur ; Sadhu, Shamir Kumar ; Hasan, Choudhury Mahmud ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 2, 2006, Pages 121~125
DOI : 10.3742/OPEM.2006.6.2.121
The crude ethanol extract of the stem bark of Trewia polycarpa (Family: Euphorbiaceae) was subjected to acetic acid induced writhing inhibition, Brine Shrimp lethality bioassay and 1, 1-diphenyl-2-picryl hydrazyl free radical scavenging assay for screening of analgesic, cytotoxic and antioxidant activity respectively. The extract produced significant (P < 0.001) writhing inhibition in acetic acid induced writhing in mice at the dose of 125, 250 and 500 mg/kg body weight respectively, which were comparable to the standard drug diclofenac sodium. The extract showed significant lethality to Brine Shrimp and the
. The extract showed prominent free radical scavenging activity (
) compare to standard drug ascorbic acid (
). The results tend to suggest that the crude ethanol extract of the bark might possess analgesic, cytotoxic and antioxidant activities or active constituent(s) responsible for the activities.
Assessment of antibacterial activity of the cardiovascular drug nifedipine
Pal, Tapas ; Dutta, Noton Kumar ; Mazumdar, Kaushiki ; Dasgupta, Asish ; L., Jeyaseeli ; Dastidar, Sujata G. ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 2, 2006, Pages 126~133
DOI : 10.3742/OPEM.2006.6.2.126
The cardiovascular drug nifedipine exhibited significant in vitro and in vivo antibacterial activity against 331 strains of bacteria belonging to three Gram-positive and twelve Gram-negative genera. The minimum inhibitory concentration of the drug, as determined both by agar and broth dilution methods, was seen to range from
against most test bacteria, including several pathogenic ones, in the in vitro studies. Nifedipine was bacteriostatic in action. in vivo studies with this drug showed that it could offer statistically significant protection (P < 0.001) to mice challenged with a virulent bacterium. Therefore, nifedipine has the potential of an antibacterial agent, which may be developed after further pharmacological studies.
Effects of bacterial LPS and DNA on the induction of IL-1β, IL-10 and IL-12 by mouse peritoneal macrophages in vitro
Samad, D. Abdel ; Abdelnoor, AM ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 2, 2006, Pages 134~143
DOI : 10.3742/OPEM.2006.6.2.134
The capacities of bacterial DNA, extracted from Salmonella typhimurium, and lipopolysaccharide (LPS), extracted from Salmonella minnesota, to activate mouse peritoneal macrophages in vitro were compared. Activation was assessed by estimating e levels of 3 cytokines, IL-10, IL-12, and
, at time intervals of 3, 6, 9, and 24 h after addition of LPS and/or DNA to macrophage cultures. Cytokine levels in culture supernatants were determined by enzyme-linked immunosorbent assay (ELISA) and cytokine mRNA levels were estimated based on band intensity in cultured cells by reverse transcriptase-polymerase chain reaction (RT-PCR). Results obtained demonstrated the ability of DNA and LPS to elicit increased production of all 3 cytokines as compared to controls. In the amount tested, LPS appeared to be a more potent inducer of IL-12, and
, whereas DNA induced higher levels of IL-10. DNA and LPS, used in combination, exhibited neither an additive nor a synergistic effect. Rather, an antagonist effect appeared to occur. RT-PCR results correlated well with ELISA.
Potential antifilarial activity of the fruit, leaf and stem extract of Melia azedarach Linn. on cattle filarial parasite Setaria cervi in vitro
Ahmed, Qamar U. ; Zaidi, S.M.K.R. ; Kaleem, M. ; Khan, N.U. ; Singhal, K.C. ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 2, 2006, Pages 144~156
DOI : 10.3742/OPEM.2006.6.2.144
The effect of aqueous and alcoholic extracts of the fruit, leaf and stem of Melia azedarach Linn. (Meliaceae) on the spontaneous movements of both the whole worm and the nerve-muscle (n.m.) preparation of Setaria (S.) cervi and on the survival of microfilariae in vitro was studied. Alcoholic extracts of fruit, leaf and stem caused inhibition of the spontaneous movements of the whole worm and the n.m. preparation of S. cervi, while only aqueous extract of fruit caused inhibition of the spontaneous movements of the whole worm and the n.m. preparation of S. cervi. The initial stimulatory effect was not observed by the aqueous and alcoholic extracts of fruit on n.m. preparation. The concentrations required to inhibit the movements of the whole worm and n.m. preparation for alcoholic extracts of fruit, leaf and stem were 250,
respectively, whereas an aqueous extract of fruit caused inhibition of whole worm and n.m. preparation at
respectively. Alcoholic extracts of the fruit, leaf and stem and aqueous extract of the fruit of M. azedarach caused concentration related inhibition on the survival of microfilariae (m.f.) of S. cervi. The
as observed after 6 h were found to be 5, 15, 10, 20 ng/ml and 10, 25, 20 and 35 ng/ml, respectively. This work was conducted in view of the exploration of potential antifilarial herbal drug.
Some pharmacological findings of non therapeutic importance of an Ayurvedic preparation Chandanasav
Chakraborty, Sharmila ; Rajia, Sultana ; Choudhuri, M. Shahabuddin Kabir ; Hossain, M. Faruk ; Sattar, Mafruhi ; Shrestha, Tripti ;
Oriental Pharmacy and Experimental Medicine, volume 6, issue 2, 2006, Pages 157~160
DOI : 10.3742/OPEM.2006.6.2.157
Chandanasav is an Ayurvedic preparation slightly reduced the gastrointestinal motility at the 15 min time interval. It increased the latent period of castor oil induced diarrhoea, slightly decreased number of stool count and lowered the purging index values. Chandanasav significantly reduced the onset and increased the duration of pentobarbital induced sleeping time. No significant analgesic effect was observed from the hot plate study Thus it may have mild constipating and central nervous system depressant activity without any effect on peripheral nervous system.