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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Oriental Pharmacy and Experimental Medicine
Journal Basic Information
Journal DOI :
Kyung Hee Oriental Medicine Research Center, Kyung Hee University
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Volume & Issues
Volume 7, Issue 4 - Dec 2007
Volume 7, Issue 3 - Sep 2007
Volume 7, Issue 2 - Jun 2007
Volume 7, Issue 1 - Mar 2007
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Lepidium sativum (Garden cress): a review of contemporary literature and medicinal properties
Mali, Ravindra G ; Mahajan, Shailaja G ; Mehta, Anita A ;
Oriental Pharmacy and Experimental Medicine, volume 7, issue 4, 2007, Pages 331~335
DOI : 10.3742/OPEM.2007.7.4.331
The utility of Lepidium sativum Linn (Garden cress) as a medicinal plant has increased many fold over a period of time. A survey of literature relating to the medicinal uses of L.sativum reveals it to be one of the widely used medicinal plants. It finds place in folklore medicine, Ayurveda, Unani and other indigenous systems of medicine. Following a large number of claims on the wide range of traditional medicinal properties of the plant, considerable efforts have been made to verify its efficacy as a curative agent through pharmacological investigations and clinical trials. In this article, a comprehensive account of the traditional uses, phytochemical investigation and therapeutic potential of Lepidium sativum is presented.
Extracts of Centaurea bornmuelleri and Centaurea huber-morathii inhibit the growth of colon cancer cells in vitro
Sarker, Satyajit Dey ; Shoeb, Mohammad ; Celik, Sezgin ; Jaspars, Marcel ; Nahar, Lutfun ; Kong-Thoo-Lin, Paul ; MacManus, Stephen M ;
Oriental Pharmacy and Experimental Medicine, volume 7, issue 4, 2007, Pages 336~340
DOI : 10.3742/OPEM.2007.7.4.336
Plants from the genus Centaurea (C.) (Family: Asteraceae alt. Compositae), widely distributed in Asia, Europe and North America, have traditionally been used in the treatment of various ailments. As a part of our on-going studies on the plants from the genus C. for their phytochemistry and biological activities, extracts of the seeds of Turkish endemic C. species, C. bornmuelleri and C. huber-morathii, were tested for their cytotoxicity towards the CaCo2 colon cancer cell line as well as for the toxicity towards the brine shrimps, using the MTT and the brine shrimp lethality assays, respectively. Among the extracts, the MeOH extract of these plants showed significant toxicity towards the brine shrimps (
, respectively). The MeOH extract of both C. species also inhibited the growth of CaCo2 colon cancer cells in the MTT assay (
=29.9 and 33.0 g/ml, respectively). As the most prominent activities in both assays were observed with the MeOH extracts, it can be assumed that the compound(s) responsible for these activities are polar in nature.
Ethanol extract of Callophyllis japonica enhances nitric oxide and tumor necrosis factor-alpha production in mouse macrophage cell line, RAW 264.7 cells
Ahn, Mee-Jung ; Park, Dal-Soo ; Yang, Won-Hyung ; Go, Gyung-Min ; Kim, Hyung-Min ; Hyun, Jin-Won ; Park, Jae-Woo ; Shin, Taek-Yun ;
Oriental Pharmacy and Experimental Medicine, volume 7, issue 4, 2007, Pages 341~347
DOI : 10.3742/OPEM.2007.7.4.341
Red seaweed (Callophyllis japonica) has long formed part of the diet of Asians, but the pharmacological properties of this plant have not been evaluated. In this study, we examined the effect of an ethanol extract of C. japonica on the generation of nitric oxide (NO) in RAW 264.7 cells. The C. japonica extract increased the generation of NO and tumor necrosis
), which were detected by the Griess method and an enzyme-linked immunosorbent assay, respectively. The increased production of NO by C. japonica extract was inhibited by
), a specific inhibitor of NO production in the L-arginine-dependent pathway, and by the nuclear
) inhibitor, pyrrolidine dithiocarbamate (
) in a dose-dependent manner. These findings demonstrate that C. japonica extract stimulates the production of NO and
in RAW 264.7 cells through the activation of
and that this extract might also inhibit the growth of the human leukemic cells.
Anti-arthritic effect of radix Paeoniae rubra herb-acupuncture: a behavioral evaluation on adjuvant-induced arthritis rats
Kim, Seung-Tae ; Hahm, Dae-Hyun ; Kim, Gun-Ho ; Park, Jong-Bae ; Han, Dong-Oh ; Park, Hi-Joon ; Shim, In-Sop ; Park, Hee-Myung ; Kim, Ki-Hyun ; Kim, Jang-Hyun ; Choi, Seong-Hun ; Lee, Hye-Jung ;
Oriental Pharmacy and Experimental Medicine, volume 7, issue 4, 2007, Pages 348~356
DOI : 10.3742/OPEM.2007.7.4.348
Radix Paeoniae rubra (PR) has been widely used for recovering acute inflammation with swelling and pain in Far East Asian countries. In order to investigate the anti-arthritic effect of PR herbacupuncture, several behavioral parameters such as body weight, knee circumference, squeaking threshold, and weight distribution ratio were investigated in an adjuvant-induced mono-arthritis rat model. We performed PR herb-acupuncture on Zusanli (ST36) of the right hind-leg once every second day for a total of 6 times from the second day of initiating arthritis with adjuvant injection, while the control group was treated with saline in the same way. In the experimental group, body weight significantly increased, knee circumference and squeaking threshold significantly decreased indicating the reduction of edema, and both the pain and inflammatory swelling of the hind paws measured by incapacitance meter decreased. Conclusively, PR herb-acupuncture has more promising effect in alleviating arthritic pain and inflammation than saline-acupuncture in rat model, hence further clinical study is required.
Association of gastric cancer with cytochrome P450 2C19 single-nucleotide polymorphisms in Koreans
Kim, Hyun-Ju ; Park, Hye-Jung ; Lee, Sang-Gyu ; Lee, Hye-Suk ; Park, Won-Cheol ; Kim, Jeong-Joong ; Oh, Gyung-Jae ; Kim, Yun-Kyung ;
Oriental Pharmacy and Experimental Medicine, volume 7, issue 4, 2007, Pages 357~362
DOI : 10.3742/OPEM.2007.7.4.357
Cytochrome P450 2C19 (CYP2C19) is a clinically important enzyme involved in the metabolism of therapeutic drugs, including (S)-mephenytoin, omeprazole, proguanil, and diazepam. Individuals are characterized as either extensive metabolizers (EM) or poor metabolizers (PM) on the basis of CYP2C19 enzyme activity. The PM phenotype occurs in 2-5% of Caucasians, but in 18-23% of Asians. To clarify the association between CYP2C19 polymorphisms and gastric cancer in Koreans, we investigated CYP2C19 genotypes (
) in 109 patients with gastric cancer and 211 controls. Normal (
) and defective alleles were detected with polymerase chain reaction/restriction enzyme analysis. CYP2C19 has three hereditary genotypes: homozygous EM, with high enzymatic activity; heterozygous EM, with moderate enzymatic activity; and PM, with no enzyme activity. We found that CYP2C19 heterozygous EM is more closely associated with gastric cancer than is homozygous EM. Because the CYP2C19 genotype varies in Koreans, a genotyping test is desirable to prevent gastropathy recurrence in patients before their doses of omeprazole are reduced during maintenance therapy.
Effect of Swietenia macrophylla seeds on normal and alloxan-induced diabetic rats
A, Maiti ; S, Dewanjee ; SC, Mandal ;
Oriental Pharmacy and Experimental Medicine, volume 7, issue 4, 2007, Pages 363~371
DOI : 10.3742/OPEM.2007.7.4.363
The methanol extract of the seed of Swietenia macrophylla (Meliaceae) seeds were investigated for its hypoglycaemic activity in normal and alloxan induced diabetic rats. Seed extract suspended in aqueous Tween 80 solution, the graded doses were administered to normal and experimental diabetic rats for 10 days. Fasting blood glucose levels, serum lipid profiles and changes in body weight were evaluated in normal rats while the hepatic marker enzymes and liver glycogen levels were evaluated additionally in diabetic rats. The diabetic groups treated with the seed extract were compared with standard drug glibenclamide. The findings showed the significant antidiabetic potential of the extract in ameliorating the diabetic conditions in diabetic rats. The extract also has hypoglycaemic action in normal rats. A toxicity study has been performed for the extract, which revealed that the extract is safe to use even at the doses of 3.2 mg/kg of body weight orally. The result from the present investigation indicates good correlation between the folk use and scientific investigation.
A single blind randomised placebo controlled clinical trial of a classical Ayurvedic formulation Ashokarista in the treatment of menorrhagia and dysmenorrhoea
Akhtar, Yasmeen ; Alamgir, Mahiuddin ; Khan, Mahmud Tareq Hassan ; Hannan, JMA. ; Choudhuri, M Shahabuddin Kabir ;
Oriental Pharmacy and Experimental Medicine, volume 7, issue 4, 2007, Pages 372~378
DOI : 10.3742/OPEM.2007.7.4.372
A well known Ayurvedic formulation Ashokarista, used for menstrual disorders has been studied in a single blind randomised placebo controlled clinical trial for the treatment of menorrhagia and dysmenorrhoea. Dysmenorrhoea and menorrhagia patients who were taking Ashokarista (20 ml twice daily) for 10 menstrual cycles had an increase in haemoglobin level. Menorrhagia treated group has shown to reduce the erythrocyte sedimentation rate level that has been increased in the menorrhagia control group. The platelet count, total count and differential count were observed unchanged in the study. The Ashokarista did not affect the SGPT and SGOT level, which signify its lack of toxicity in hepatic function. The treated menorrhagic patients showed an increase in serum albumin content and decrease in blood clotting time, whereas the serum protein content was observed unchanged. There was a significant increase in both serum cholesterol and triglyceride level, which usually associated with the use of oral contraceptives. No major side effects were observed by the clinicians during the study.
Bioactivity of two medicinal plant Xylocarpus granatum Koen. (Meliaceae) and Sarcolobus globosus Wall. (Asclepiadaceae) of Sundarbans mangrove forest
Alamgir, M ; Rob, Ma ; Kundu, DC ; Joy, JHK ; Sarder, MM ;
Oriental Pharmacy and Experimental Medicine, volume 7, issue 4, 2007, Pages 379~384
DOI : 10.3742/OPEM.2007.7.4.379
Two medicinal plant of Sundarbans mangrove forest has been tested for the evaluation of growth inhibitory and antibacterial activity. The methanol extract of Xylocarpus granatum stem bark showed potent wheat rootlet (
) and shoot (
) growth inhibitory activity in a concentration related manner. The growth inhibitory activity was markedly decreased in residual methanol extract. The methanol extract showed antibacterial activity (MIC > 3 mg/ml) against Bacillus subtilis, Staphylococcus aureous and Proteus vulgaris. The residual methanol extract did not show any antibacterial activity. The results suggest the bioactive principle(s) of Xylocarpus granatum may be relatively non polar compound(s). The methanol extract and residual methanol extract of Sarcolobus globosus stem showed poor wheat rootlet and shoot growth inhibitory activity and no antibacterial activity.
Preliminary antioxidant, antibacterial and cytotoxic activities of Momordica charantia Linn leaf
Rahman, Shafiur ; Iqbal, Asif ;
Oriental Pharmacy and Experimental Medicine, volume 7, issue 4, 2007, Pages 385~389
DOI : 10.3742/OPEM.2007.7.4.385
We undertook the present study to evaluate different pharmacological as antioxidant, antibacterial and cytotoxic activities of the crude ethanolic extract of Momordica charantia (Family: Cucurbitaceae) leaves. The antioxidant property of the extract was assessed by 1, 1-diphenyl-2-picryl hydrazyl free radical scavenging assay. The extract showed antioxidant activity where
for standard drug ascorbic acid. The extract showed a broad spectrum of antibacterial activity against all the tested gram positive and gram-negative bacteria where Staphylococcus epidermidis, Staphylococcus aureus, Salmonella typhi and Shigella dysenteriae were prominent. And the zones of inhibition were ranging from 8-15 mm for all the tested bacteria. Its cytotoxic property was evaluated by brine shrimp lethality bioassay. The extract showed significant lethality and the
Antinociceptive profile of the ethanolic extract of andrographis paniculata in mice
Sulaiman, MR ; Sainan, S ; Zakaria, ZA ; Somchit, MN ; Israf, DA ; Moin, S ; Mohamad, TA Tengky ;
Oriental Pharmacy and Experimental Medicine, volume 7, issue 4, 2007, Pages 390~394
DOI : 10.3742/OPEM.2007.7.4.390
The present study was conducted to evaluate the analgesic activity of ethanolic extract of Andrographis paniculata (AP) in mice. The analgesic investigations were carried out using the acetic acid-induced abdominal writhing and the hot-plate tests. It was demonstrated that intraperitoneal (i.p.) administration of the extract at a dose of 30, 100, 300, 500 mg/kg, produced significant inhibition of abdominal constriction induced with 0.6% (v/v) acetic acid in dosedependent manner. It also demonstrated that the extract produced significant dose-dependent increase in the time of latency to a discomfort reaction in the hot-plate model. In addition, the analgesic effect of the ethanolic extract of AP was significantly reversed by a non-specific opioid receptor antagonist, naloxone. These results indicate that AP has an analgesic effect that was mediated through opioid receptors.
Evaluation of analgesic and antiinflammatory activity of Ophiorrhiza nicobarica, an ethnomedicine from Nicobar Islands, India
Chattopadhyay, Debprasad ; Das, Sonali ; Mandal, Asit Baran ; Arunachalam, G ; Bhattacharya, SK ;
Oriental Pharmacy and Experimental Medicine, volume 7, issue 4, 2007, Pages 395~408
DOI : 10.3742/OPEM.2007.7.4.395
This study reports the analgesic, anti-inflammatory and membrane-stabilizing property of alcoholic extract of Ophiorrhiza nicobarica (ON), a wild herb, used as an anti-infective ethnomedicine of Nicobarese and Shompen tribes of Great Nicobar Island, India. We for the first time investigated the analgesic and antiinflammatory potential of this herb in acute, subacute and chronic model of inflammation in Swiss albino mice and Wistar albino rats, along with sheep RBC-induced sensitivity and membrane stabilization. The acetic acid induced writhing, tail flick and tail immersion tests are used as a model for evaluating analgesic activity; while the carrageenin-induced paw oedema was used as the model for acute inflammation, dextran-induced oedema as sub-acute and cotton-pellateinduced granuloma as chronic inflammatory model. The probable mode by which ON mediate its effect on inflammatory conditions was studied on sheep RBC-induced sensitivity and membrane stabilization. The in vitro results revealed that the ON extract possesses significant (P < 0.05) dose dependent analgesic and antiinflammatory activity at 200 and 300 mg/kg and its fractions at 50 mg/kg, p.o. respectively, compared to the control groups. However, the extract failed to exhibit membrane-stabilizing property as it unable to reduce the level of haemolysis of RBC exposed to hypotonic solution. The acute toxicity studies of ON extract in rats and mice revealed that the extract was nontoxic even up to 3.0 g/kg body weight of the animals, with a high safety profile. We have isolated ursolic acid,
-sitosterol and harmaline respectively, from the bioactive part of the extract. The results indicated that the O. nicobarica is indeed beneficial in primary health care, and suggest that its anti-inflammatory activity may not be related to membrane-stabilization.
Effect of a polyherbal formulation on anxiety and behaviour mediated via monoamine neurotransmitters
Balaraman, R ; Mohan, M ; Aurangabadkar, VM ; Jadhav, GB ; Austin, Anoop ; Thirugnanasampathan, Thirugnanasampathan ;
Oriental Pharmacy and Experimental Medicine, volume 7, issue 4, 2007, Pages 409~417
DOI : 10.3742/OPEM.2007.7.4.409
We investigated the effect of Arogh, a polyherbal formulation (PHF) on animal models of anxiety based on exploratory behavior. The anxiolytic activity of polyherbal formulation (30, 100, 300 and 500 mg/kg) was studied using various behavioural paradigms such as elevated plus maze (EPM), light/dark apparatus (LDA), open field apparatus (OFA), hole board apparatus (HBA). Diazepam (1 mg/kg) was used as a standard anxiolytic drug. The effect of PHF (100 and 300 mg/kg) on serotonin, dopamine and noradrenaline mediated behaviour was studied by lithium induced head twitches in rats, haloperidol induced catalepsy in mice and clonidine induced hypothermia in rats respectively. In EPM, PHF (100, 300 and 500 mg/kg) significantly (P < 0.05) increased the time spent in open arms and the number of entries in open arms. In LDA, PHF (100, 300 and 500 mg/kg) significantly (P < 0.05) increased the time spent in lit zone. In OFA, PHF (100, 300 and 500 mg/kg) significantly (P < 0.05) increased the number of assisted rearing and the number of squares traversed. In HBA, PHF (100, 300 and 500 mg/kg) significantly (P < 0.05) increased the number of head poking. In lithium induced head twitches, PHF (100 and 300 mg/kg) significantly (P < 0.05) decreased the number of head twitches. In haloperidol induced catalepsy, PHF (300 mg/kg) decreased the duration of catalepsy significantly (P < 0.05) at 60 min. In clonidine-induced hypothermia, PHF (300 mg/kg) did not modify the effect. Drugs must be carefully assessed on EPM test and therefore in the present study EPM is supported by other tests. Present study indicates that Arogh, a polyherbal formulation possess anxiolytic activity. It diminished serotonergic transmission and decreased the duration of catalepsy indicating potentiation of dopaminergic transmission. Thus, Arogh a polyherbal formulation contains bioactive principles which possess anxiolytic activity and modified 5-HT and DA mediated behaviour.
Antihyperglycemic activity of Biophytum sensitivum (L.) DC in alloxan diabetic rats
Mishra, Moumita ; Bandyopadhyay, Durba ; Pramanik, Kartick Chandra ; Chatterjee, Tapan Kumar ;
Oriental Pharmacy and Experimental Medicine, volume 7, issue 4, 2007, Pages 418~425
DOI : 10.3742/OPEM.2007.7.4.418
The study was to evaluate the antihyperglycemic activity of Biophytum sensitivum (L.) DC in different extracts. Albino Wistar rats with alloxan hydrate induced diabetes were divided into 7 groups of 6 each. Both aqueous and methanolic extract of Biophytum sensitivum were prepared and given individually at different doses to different batches of rats (both normal and diabetic rats) after an overnight fast. Methanolic extract at the dose of 200 mg/kg body weight showed maximum blood glucose lowering effect in diabetic rats. The same dosages did not produce any hypoglycemic activity in normal rats. The antihyperglycemic activity of Biophytum sensitivum was compared with a standard drug Glibenclamide, an oral hypoglycemic agent. The above results suggest that maximum hypoglycemic effect was found only with a dose of up to 200 mg/kg b.w. of methanolic extract which is therefore the optimum dose for hypoglycemia and was used in all the experiments of the present study.
Antitumor and antioxidant status of Galega purpurea root in Ehrlich ascites carcinoma bearing Swiss albino mice
Gupta, M ; Mazumder, UK ; Gomathi, P ;
Oriental Pharmacy and Experimental Medicine, volume 7, issue 4, 2007, Pages 426~435
DOI : 10.3742/OPEM.2007.7.4.426
The present study was designed to determine the antitumor and antioxidant properties of methanol extract from the root of Galega purpurea (Papilionaceae) (MEGP) against Ehrlich Ascites Carcinoma (EAC) bearing Swiss albino mice. Acute and short-term toxicity studies were performed initially in order to ascertain the safety of MEGP. The effect of MEGP on the growth of transplantable murine tumor, life span of EAC bearing hosts and simultaneous alterations in the hematological profile and liver biochemical parameters (lipid peroxidation, antioxidant enzymes) were estimated. The MEGP showed decrease in tumor volume, packed cell volume and viable cell count and increases the nonviable cell count and mean survival time thereby increasing life span of EAC tumor bearing mice. Hematological profile reverted to more or less normal levels in extract treated mice. Treatment with MEGP decreased the levels of lipid peroxidation and increased the levels of glutathione, superoxide dismutase and catalase. The results suggested that the methanol extract of Galega purpurea root exhibited antitumor effect by modulating lipid peroxidation and augmenting antioxidant defense system in EAC bearing mice.
A case report on regression of hepatocellular carcinoma treated with herbal medicine
Han, Sung-Soo ; Kim, Jung-Sun ; Park, Bong-Ky ; Yoo, Hwa-Seung ;
Oriental Pharmacy and Experimental Medicine, volume 7, issue 4, 2007, Pages 436~440
DOI : 10.3742/OPEM.2007.7.4.436
Hepatocellular carcinoma (HCC) is one of the most prevalent malignant diseases worldwide and a leading cause for death in Asia, where the major risk factors are chronic hepatitis B virus and hepatitis C virus infection. Because most HCC patients die within 3 to 6 months from the time of diagnosis, searching for a new treatment has become more urgent for HCC than other cancers because there is no existing effective systematic therapy. In Korea and Asia, traditional herbal medicine is frequently administered to patients with advanced HCC. We present a HCC case where complete regression was observed after taking herbal medicine. Since the specific mechanism is unknown, we cannot determine whether the herbal preparation had a direct effect on the regression of HCC. Nevertheless, this case provides us a reason and hope for further research.
Preliminary in vitro evaluation of some traditional Ayurvedic antacids
Nahar, Nusratun ; Choudhuri, M Shahabuddin Kabir ; Alamgir, Mahiuddin ;
Oriental Pharmacy and Experimental Medicine, volume 7, issue 4, 2007, Pages 441~443
DOI : 10.3742/OPEM.2007.7.4.441
A preliminary in vitro acid neutralizing capacity test of twelve traditional Ayurvedic antacids were performed in this study. Five traditional preparations of ash of conch shell, ash of oyster, ash of pearl, limestone, and ash of cowrie showed high acid neutralizing capacity similar to standard antacid combination of
. Among these the ash of conch shell found the highest acid neutralizing capacity. The ash of tamarind and ash of Achyranthus aspera showed moderate acid neutralizing capacity. The acid neutralizing capacity of red ochre; ash of iron; mixture of niter, alum and ammonium chloride; saltpeter; and ash of mica found below the USP 23 limit.
Analgesic activity of the ethanolic extract of Aphanamixis polystachya bark
Hasan, Faizul ; Rouf, Razina ; Barua, Juwel ; Uddin, Shaikh Jamal ; Shilpi, Jamil Ahmad ;
Oriental Pharmacy and Experimental Medicine, volume 7, issue 4, 2007, Pages 444~446
DOI : 10.3742/OPEM.2007.7.4.444
Ethanolic extract of Aphanamixis polystachya bark was used to investigate its analgesic activity by acetic acid induced writhing in mice. The bark extract exhibited statistically significant and dose dependent analgesic activity in mice. The bark extract at the doses of 250 and 500 mg/kg body weight showed 40.69% and 62.07% writhing inhibition respectively in mice whereas diclofenac-Na produced 75.17% writhing inhibition as a positive control.