Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Oriental Pharmacy and Experimental Medicine
Journal Basic Information
Journal DOI :
Kyung Hee Oriental Medicine Research Center, Kyung Hee University
Editor in Chief :
Volume & Issues
Volume 8, Issue 4 - Dec 2008
Volume 8, Issue 3 - Sep 2008
Volume 8, Issue 2 - Jun 2008
Volume 8, Issue 1 - Mar 2008
Volume 7, Issue 5 - Feb 2008
Selecting the target year
Evidence-based herbal medicine in efficacy and safety assessments
Park, Jin-Han ;
Oriental Pharmacy and Experimental Medicine, volume 8, issue 2, 2008, Pages 103~110
DOI : 10.3742/OPEM.2008.8.2.103
Herbal medicine is the use of medicinal plants for prevention and treatment of diseases: it ranges from traditional and popular medicines of every country to the use of standardized and tritated herbal extracts. Generally cultural rootedness enduring and widespread use in a traditional medical system may indicate safety, but also efficacy of treatments, especially in herbal medicine where tradition is almost completely based on remedies containing active principles at very low and ultra low concentrations or relying on magical-energetic principles. The efficacy and safety assessments of medicines, whether modern or herbal, invariably encounter challenges or problems during the course of pre-clinical and clinical research. Some of the challenges in evidence-based herbal medicinal research are unique, and the researcher must be cognizant of them in order to safeguard the quality of the data obtained. Key challenges are: the quality of raw materials; appropriateness of biological/pharmacological activity assessment methodology, and data interpretation; standardization methodology; pharmacokinetics and bioavailability of active constituents and metabolites; clinical dosage formulation/production; and clinical study designs and outcome measures.
Reversible histoarchitecture study of testis and cauda epididymis and changes in cauda epididymal epithelial cell types on treatment with benzene extract of Ocimum sanctum leaves in albino rats
Ahmed, Mukhtar ; Ahamed, R Nazeer ; Aladakatti, RH ; Deepthi, KR ;
Oriental Pharmacy and Experimental Medicine, volume 8, issue 2, 2008, Pages 111~124
DOI : 10.3742/OPEM.2008.8.2.111
In the present study, an attempt has been made to assess whether the effect of benzene extract of Ocimum sanctum leaves on the ultrastructural changes in the epithelial cells of the cauda epididymis, its subsequent recovery in the seminiferous epithelium and fertility of male albino rats. Wistar strain male albino rats were orally administered benzene extract of 250 mg/kg body weight of O. sanctum leaves followed by subsequent recovery maintaining suitable controls for 48 days. Results indicate decrease in the weights of testis, epididymis and seminal vesicles. Other accessory organs were not affected. Total count, cell and nuclei diameters of germ cells and Leydig cells were reduced. Cauda epididymis exhibited significant reduction in epithelial height and nuclei diameter of epithelial cells. Cells showed vacuolization with exhibit of signs of degeneration. Ultra study revealed that, in general, the cauda epididymis was affected and in particular, the principal, clear and basal cells were highly disturbed. Further, there was decrease in the size of lipid droplets, mitochondria, Golgi complex, endoplasmic reticulum and accumulation of lysosomal bodies. Fertility performance test showed no implantation in female rats mated with O. sanctum treated rats. Moreover, their recovery after withdrawal of treatment was observed suggesting that the effect of the treatment is transient and reversible. A recovery period resulted in normal spermatogenesis and fertility, suggesting reversible antispermatogenic and antifertility effects of the plant.
Screening of KMU-4, 6, 7 on inflammatory responses in IFN-γ and LPS-induced mouse peritoneal macrophages
Na, Ho-Jeong ; Jeong, Hyun-Ja ; Ahn, Jong-Woong ; Um, Jae-Young ; Kim, Hyung-Min ; Hong, Seung-Heon ;
Oriental Pharmacy and Experimental Medicine, volume 8, issue 2, 2008, Pages 125~129
DOI : 10.3742/OPEM.2008.8.2.125
Korean Marine Plants (KMU-4, 6, 7) obtained from an herb which widely used in medicine for the treatment of a variety of pathologies. In this study, using mouse peritoneal macrophages, we have examined whether KMU-4, 6, 7 affects nitric oxide (NO) and COX-2 induced IFN-
and LPS and cell viability. KMU-6 inhibits IFN-
and LPS-induced NO. We found that KMU-6 had a little effect on COX-2 expression. These finding means that KMU-6 can be used in controlling macrophages mediated inflammatory disease. The present results indicate that KMU-6 has an inhibitory effect on the production of NO through down-regulation of COX-2 expression in LPS stimulated mouse peritoneal macrophages.
Evaluation of antidiarrhoeal activity of Cardamom (Elettaria cardamomum) on mice models
Rahman, Tasmina ; Rahman, Khandaker Ashfaqur ; Rajia, Sultana ; Alamgir, Mahiuddin ; Khan, Mahmud Tareq Hassan ; Choudhuri, M Shahabuddin Kabir ;
Oriental Pharmacy and Experimental Medicine, volume 8, issue 2, 2008, Pages 130~134
DOI : 10.3742/OPEM.2008.8.2.130
Diarrhoea is a major health care problem in developing countries. Elettaria cardamomum Maton fruits, commonly known as cardamom are widely used for flavoring purposes in food. In this study we evaluated the antidiarrhoeal activity of hot water extract of cardamom against experimental diarrhoeal models on mice. Cardamom extract showed significant antidiarrhoeal activity against castor oil and magnesium sulphate induced models. Whereas, the gastrointestinal motility was slightly increased.
Antioxidant and free radical scavenging activities of Cleome rutidosperma
Bose, Anindya ; Mondal, Sumanta ; Gupta, Jayanta Kumar ; Ghosh, Tirtha ; Debbhuti, Debabrata ; Si, Sudam ;
Oriental Pharmacy and Experimental Medicine, volume 8, issue 2, 2008, Pages 135~145
DOI : 10.3742/OPEM.2008.8.2.135
The study was aimed at evaluating the antioxidant and free radical scavenging activities of ethanolic extract and its fractions of Cleome rutidosperma. The antioxidant activity, reducing power, total phenolic content, total flavonoid content, superoxide anion scavenging activity, nitric oxide anion scavenging activity, in vitro antilipid peroxidation activity and in vitro non-enzymatic hemoglobin glycosylation were studied. The results obtained in the study indicate that Cleome rutidosperma is a potential source of natural antioxidant. All the parameters were found to be concentration dependent and increased with increasing amounts of sample. Flavonoids, phenolic compound like tannins, terpenoids may be responsible for the antioxidant activity of the plant. Variation of solubility parameters in various models may be attributed to non-linearity of activity of ethanol extract fractions models. Further investigation on the isolation and identification of antioxidant component(s) in the plant may lead to chemical entities with potential for clinical use.
Silymarin, a flavonoid antioxidant, protects streptozotocin-induced lipid peroxidation and β-Cell damage in rat pancreas
Sharma, Manju ; Anwer, Tarique ; Pillai, K K ; Haque, Syed Ehtaishamul ; Najmi, A K ; Sultana, Yasmin ;
Oriental Pharmacy and Experimental Medicine, volume 8, issue 2, 2008, Pages 146~153
DOI : 10.3742/OPEM.2008.8.2.146
The present study is aimed at finding the influence of silymarin (a flavonoid) (25 mg/kg & 50 mg/kg) in streptozotocin (STZ)-induced diabetic rats. Type 2 diabetes was induced by single intraperitoneal injection of STZ (100 mg/kg) to 3 days old rat pups. Silymarin was administered for 15 days after the animals were confirmed diabetic (75 days after STZ injection). Blood glucose, glycosylated hemoglobin (
), lipid peroxides (LPO) levels and reduced glutathione (GSH) contents in pancreas and liver were estimated following the established procedures. Biochemical observations were further substantiated with histological examination of pancreas. Blood glucose and
levels, which were elevated by STZ, were lowered to physiological levels by the administration of silymarin. The levels of LPO were significantly increased in STZ-induced diabetic rats. Silymarin reduced the LPO levels in both pancreas and liver. GSH contents which were reduced significantly in pancreas and liver of STZ-induced diabetic rats were brought back to near normal levels by silymarin treatment. Multifocal necrotic and degenerative changes of pancreas in STZ-diabetic rats were minimized to near normal morphology by administration of silymarin as evident by histopathological examination. Silymarin showed a dose dependent protective effect on STZ-induced
-cell damage. It could be attributed to the antioxidative and free radicals scavenging properties of the flavonoid. Thus, it may be considered as a natural antioxidant with potential therapeutic application in the treatment of type 2 diabetes.
Antitumor effect of Careya arborea against Ehrlich ascites carcinoma with reference to lipid peroxidation and enzymatic and non enzymatic antioxidant system in Swiss albino mice
Kumar, R Sambath ; Sivakumar, T ; Senthil, V ; Murthy, N Venkateswara ; Balasubramaniam, V ; Sabi, R Kanaga ; Sundram, R. Shanmuga ; Perumal, P ; Mazumder, U K ; Gupta, M ;
Oriental Pharmacy and Experimental Medicine, volume 8, issue 2, 2008, Pages 154~163
DOI : 10.3742/OPEM.2008.8.2.154
The methanol extract of stem barks of Careya arborea Roxb. (MECA) (Family- Myrtaceae) was evaluated for antitumor activity and antioxidant status against Ehrlich's Ascites Carcinoma (EAC) bearing Swiss albino mice. After 24 h of tumor inoculation the MECA was administered at the doses of 50, 100 and 200 mg/kg body weight/mice/day for 14 days. After the last dose and 18 h fasting mice were sacrificed. The effect of MECA on the growth of transplantable murine tumor, life span of EAC bearing hosts, hematological profiles, serum and liver biochemical parameters were estimated. The MECA showed significant (P < 0.01) decrease in ascites volume, packed cell volume and viable cell count and prolonged the life span of EAC tumor bearing mice. Hematological profiles reverted to more or less normal levels in extract treated mice. The MECA also produced protective effect by decreasing the activity of serum enzymes, bilirubin and increase the protein and uric acid levels. MECA significantly (P < 0.05) decreased the levels of lipid peroxidation, while significantly (P < 0.05) increased the levels of glutathione content, vitamin C, vitamin E, superoxide dismutase and catalase CAT. The results indicate that MECA exhibited significant antitumor and antioxidant activity in EAC bearing mice.
Antioxidant and hepatoprotective action of the crude ethanolic extract of the flowering top of Rosa damascena
Alam, MA ; Nyeem, MAB ; Awal, MA ; Mostofa, M ; Alam, MS ; Subhan, N ; Rahman, M Mostafizur ;
Oriental Pharmacy and Experimental Medicine, volume 8, issue 2, 2008, Pages 164~170
DOI : 10.3742/OPEM.2008.8.2.164
The hepatoprotective activity of the alcoholic extract of Rosa damascena was studied against paracetamol induced acute hepatotoxicity in rats. Liver damage was assessed by estimating serum enzyme activities of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and histopathology of liver tissue. Pre- and post-treatment with ethanolic extracts showed a dose-dependent reduction of paracetamol induced elevated serum levels of enzyme activity. The mechanism underlying the protective effects was assayed in vitro and the R. damascena extracts displayed dosedependent free radical activity using DPPH (
) and TBA method. The hepatoprotective action was confirmed by histopathological observation. The ethanolic extracts reversed paracetamol induced liver injury. These results suggest that the hepatoprotective effects of R. damascena extracts are related to its antioxidative activity.
In vitro and in vivo antidiarrhoeal activity of epigallocatechin 3-gallate: a major catechin isolated from indian green tea
Bandyopadhyay, Durba ; Dutta, Pradeep Kumar ; Dastidar, Sujata G ; Chatterjee, Tapan Kumar ;
Oriental Pharmacy and Experimental Medicine, volume 8, issue 2, 2008, Pages 171~177
DOI : 10.3742/OPEM.2008.8.2.171
Epigallocatechin 3-gallate (EGCG), one of the major catechins of tea, was isolated from the decaffeinated, crude methanolic extract of Indian green tea (Camellia sinensis L. O. Kuntze) using chromatographic techniques. EGCG was then screened for antidiarrhoeal activity against 30 strains (clinical isolates) of V. cholerae, which is a well known Gram negative bacillus functioning as the pathogen of cholera. V. cholerae strains like V. cholerae 69, 71, 83, 214, 978, 1021, 1315, 1347, 1348, 569B and ATCC 14033 were inhibited by EGCG at a concentration of
whereas V. cholerae 10, 522, 976 were even more sensitive, being inhibited at
level. However, V. cholerae DN 16, DN 26, 30, 42, 56, 58, 113, 117, 564, 593, 972 and ATCC 14035 were inhibited at
level of EGCG. Only four strains were inhibited at
. In this study the isolated compound was found to be bacteriostatic in its mechanism of action. In the in vivo experiment using the rabbit ileal loop model two different dosages of EGCG (
) were able to protect the animals when they were challenged with V. cholerae 569B in the ileum.
Preparation and characterization of ibuprofen-loaded alginate microspheres using ethylenediamine as a crosslinker
Maiti, Sabyasachi ; Sa, Biswanath ;
Oriental Pharmacy and Experimental Medicine, volume 8, issue 2, 2008, Pages 178~186
DOI : 10.3742/OPEM.2008.8.2.178
In this study, ionotropic gelation method was used for the preparation of ibuprofen-loaded calcium alginate (CALG) and ethylenediamine (EDA) treated calcium alginate (EDA-CALG) microspheres. The effect of EDA-treatment on drug entrapment efficiency, particle size, morphology, swelling behavior and in vitro release characteristics of the microspheres was investigated by varying its concentration from 0.5 to 2% (v/v). The reduction in drug entrapment efficiency by a maximum of 44.60% was noted for EDA-CALG microspheres compared to untreated CALG microspheres. The particle size and swelling index of EDA-CALG microspheres were reduced with increasing EDA concentration. All the microspheres were observed to retain their spherical shapes with rough surfaces. EDA-CALG microspheres prepared using 1% and 2% v/v EDA, released almost all of its content within 7 h in pH 6.8 phosphate buffer, however, CALG microspheres were found to release the same within 3 h. The intensity of melting endothermic peak of ibuprofen reduced significantly at lower drug load as experienced from DSC thermograms. The FT-IR spectrum of pure ibuprofen, ibuprofen-loaded CALG and EDA-CALG microspheres showed the characteristic band of C = O stretching vibration of ibuprofen. Hence, this study revealed that EDA can be employed for the preparation of ibuprofen-loaded CALG microspheres to retard the drug release to some extent.
Analgesic and antidiarrhoeal activities of Treama orientalis Linn. in mice
Uddin, Sarder Nasir ; Uddin, Khan Mohammad Ahsan ; Ahmed, Firoz ;
Oriental Pharmacy and Experimental Medicine, volume 8, issue 2, 2008, Pages 187~191
DOI : 10.3742/OPEM.2008.8.2.187
Trema orientalis Linn. is commonly grown in many parts of Bangladesh. Its leaves have been used for analgesic and anti-diarrhoeal activity in traditional medicine. This study evaluates the potential analgesic and anti-diarrhoeal activity of methanol and aqueous extracts of leaves in experimental acetic acid induced writhing and castor oil induced diarrhoea in mice. The aqueous extract of leaves showed significant (P < 0.001) analgesic effect in acetic acid induced writhing in mice at a dose of 500 mg/kg body weight In castor oil induced antidiarrhoeal screening both extract increased latent period (P < 0.025) and decrease the number of stool (P < 0.025) at the dose of 500 mg/kg body weight comparable with that of the standard drug loperamide. The results provide a support for the use of this plant in traditional medicine and suggest its further investigation.
Evaluation of antifilarial activity of Berberis aristata roots
Rizvi, Waseem ; Kumar, Anil ; Ahmad, Razi ; Shahid, Mohammad ; Shazad, Anwar ; Haider, Nakhat ;
Oriental Pharmacy and Experimental Medicine, volume 8, issue 2, 2008, Pages 192~195
DOI : 10.3742/OPEM.2008.8.2.192
The effect of aqueous and alcoholic extract of roots of Berberis aristata was studied on the spontaneous movements of the whole worm (w.w) preparation and nerve muscle (n.m) complex of cattle filarial parasite Setaria cervi and on the survival of microfilariae. Only aqueous extract could inhibit the spontaneous movements of S. cervi, characterized by initial stimulation followed by reversible paralysis. The concentration required to produce similar effect on n.m complex was less as compared to the w.w. The lethal concentration 50 and lethal concentration 90 for aqueous extract were 48 ng/ml and 65 ng/ml.
Evaluation of the analgesic and anti-inflammatory properties of methanol extract of Artanema sesamoides Benth roots in animal models
Gupta, Malaya ; Mazumder, UK ; Selvan, V Thamil ; Manikandan, L ; Senthilkumar, GP ; Suresh, R ; Gomathi, P ; Kumar, B Ashok ;
Oriental Pharmacy and Experimental Medicine, volume 8, issue 2, 2008, Pages 196~203
DOI : 10.3742/OPEM.2008.8.2.196
The methanol extract of the root of Artanema sesamoides Family Scrophuilariaceae (MEAS) was investigated for possible analgesic and anti-inflammatory effects in animals. Three models were used to study the extract effects on nociception, which were acetic acid-induced writhing response, hot-plate method and the tail flick test in mice. The antiinflammatory effects were evaluated using carrageenan, dextran, histamine and serotonin induced rat paw oedema (acute) and cotton pellet induced granuloma (chronic) models in rats. Results of the study revealed that the extract exhibited significant (P < 0.001) analgesic effect at a dose of 50, 100 and 200 mg/kg b.w p.o in mice in all the models. In acute model, the MEAS also exhibited significant (P < 0.001) antiinflammatory effect in all the above mentioned doses. In chronic model (cotton pellet induced granuloma) the MEAS 200 mg/kg and indomethacin 10 mg/kg showed that inhibition of granuloma formation 25.0% and 47.7% respectively (P < 0.001). The MEAS and indomethacin were effectively preventing the transudation of the fluid. Thus, the present study revealed that the methanol extract of the root of Artanema sesamoides exhibited significant analgesic and antiinflammatory activity.
Cytotoxicity and antinociceptive activity of Jasminum sambac leaves
Islam, Md Rahatul ; Rouf, Razina ; Barua, Juwel ; Uddin, Shaikh Jamal ; Alamgir, Mahiuddin ;
Oriental Pharmacy and Experimental Medicine, volume 8, issue 2, 2008, Pages 204~206
DOI : 10.3742/OPEM.2008.8.2.204
The ethanolic extract of Jasminum sambac leaves were tested for its cytotoxicity and possible antinociceptive activity in experimental animals. The extract showed potent cytotoxic activity in brine shrimp lethality assay and the LC50 was found only 25 mg/ml. The extract significantly and dose dependently inhibited the acetic acid induced writhing in mice (56.83%, P < 0.001 and 43.17%, P < 0.001 for 500 and 250 mg/kg body weight, respectively). The results supported its traditional uses.