Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Applied Biological Chemistry
Journal Basic Information
Journal DOI :
The Korean Society for Applied Biological Chemisty
Editor in Chief :
Volume & Issues
Volume 46, Issue 4 - Dec 2003
Volume 46, Issue 3 - Sep 2003
Volume 46, Issue 2 - Jun 2003
Volume 46, Issue 1 - Mar 2003
Selecting the target year
Improvement of In-gel Digestion and Recovery of Peptides for Peptide Mass Fingerprinting
Oh, Ji-Sun ; Lim, Jin-Kyu ;
Journal of Applied Biological Chemistry, volume 46, issue 1, 2003, Pages 1~5
Protein spots in 2-DE gel stained with Coomassie Brilliant Blue R-250 were in-gel digested. The resulting peptides were extracted by simple diffusion at elevated temperature, repeated freezing and thawing, vigorous vortexing, sonication, increasing surface area or combinations of these treatments to enhance the recovery of peptides from the tryptic in-gel digestion. Among the methods tested, the sonication on the crushed gel yielded the highest peptide recovery and detectability on MALDI-TOF mass spectrometry. In addition to sonication the surface area of the gel should be maximized to achieve high peptide recovery from gel. Judging from the detectability and the sequence coverage of the peptides to the corresponding protein, the sonication method, compared to the simple diffusion method, showed superiority in peptide mass fingerprinting, particularly useful for the peptide mass fingerprinting of the proteins that are expressed at low level (<0.5 pmol) in tissues or cells, using MALDI-TOF mass spectra.
-Glucosidase from Thermomonospora fusca: Purification and Biochemical Characterization
Ryu, Il-Hwan ; Kim, Jung-Eun ; Lee, Kap-Sang ;
Journal of Applied Biological Chemistry, volume 46, issue 1, 2003, Pages 6~11
-glucosidase obtained from a strain of thermophillic actinomyces Thermomonospora fusca (ATCC 27730) was successively purified by ethanol precipitation, ion exchange chromatography, and gel-filtration chromatography with an overall purification of about 4.39-fold.
-Glucosidase was judged as a single protein band by polyacrylamide gel electrophoresis, and its molecular weight was determined to be 45,000 by SDS-polyacrylamide gel electrophoresis and gel-filteration column chromatography. Optimal pH and temperature for the enzyme activity were 6.5 and
, respectively. The pH and temperature stability were within a stable at a pH range of 5.0 to 7.5 and at
, respectively. Glucose, the reaction product inhibited
-Glucosidase was activated by
, and inactivated by
, iodine, EDTA, and p-CMB. The apparent value of
-glucosidase was 0.76 mM.
Compositional Characterization of Cacao (Theobroma cacao L.) Hull
Chung, Byung-Yeoup ; Iiyama, Kenji ; Han, Kang-Wan ;
Journal of Applied Biological Chemistry, volume 46, issue 1, 2003, Pages 12~16
Chemical composition and structural characteristics of cacao (Theobroma cacao L.) hull were investigated. Holocellulose, Klason residue, total amino acid, lipid, ash, and glucose contents were 334, 320, 96, 99, 93, and 174 g
, respectively. Other neutral sugars ranged 21-48 g
NMR spectrum of the purified polyphenols of cacao hull suggested that the Klason residue is mainly composed of tannin-like materials, together with small amounts of lignin. The results for total amino acid (96 g
), nitrogen contents of the Klason residue (25 g
) and samples treated with various surfactants (21-27 g
), suggested the presence of polyphenol-protein complexes in cacao hull.
Leaching of Al, Base-cations, and Anions by Simulated Acid Rain with Varying
Ratios in Reclaimed Saline Soils
Cho, Jae-Young ; Han, Kang-Wan ; Nishiyama, Masaya ; Matsumoto, Satoshi ;
Journal of Applied Biological Chemistry, volume 46, issue 1, 2003, Pages 17~24
The purpose of this study is to demonstrate the relative influence of the anion composition of simulated acid rain on the leaching of Al, cations, and anions in three reclaimed saline soils. Reclaimed saline soils used for this study were collected from surface soils of the Saemangeum (SMG) and Koheung (KH) soil in Korea and the Hachirogata (HCG) soil in Japan. The simulated acid rain at pH 3.0 was prepared with nitrate-to-sulfate mole ratios of 0.75:0.25, 0.5:0.5, 0.25:0.75, and control (pH = 5.7). The cumulative leaching amount of cations and anions in the SMG and KH soil show no significant differences among the four treatments. However, those of cations and anions in the HCG soil possessing variable charge increased as the nitrate content of the simulated acid rain became greater. The cumulative leaching amount of aluminum in reclaimed saline soil increased as the nitrate content of the simulated acid rain became greater.
Fungicidal Activity of ar-Turmerone Identified in Curcuma longa Rhizome against Six Phytopathogenic Fungi
Lee, Hoi-Seon ; Choi, Kyung-Ja ; Cho, Kwang-Yun ; Ahn, Young-Joon ;
Journal of Applied Biological Chemistry, volume 46, issue 1, 2003, Pages 25~28
Fungicidal activity of materials derived from the rhizome of turmeric, Curcuma longa, against six phytopathogenic fungi was examined using a whole plant bioassay. The biologically active constituent isolated from the Curcuma rhizome was characterized as the sesquiterpene ketone ar-turmerone by spectroscopic analyses. ar-Turmerone at 500 ppm was highly suppressive to Phytophthora infestans and Erysiphe graminis but was almost ineffective against Magnaporthe grisea, Rhizoctonia solani, Botrytis cinerea, and Puccinia recondita. The Curcuma rhizome-derived ar-turmerone merits further study as a potential disease control agent or as a lead compound.
Cytotoxic Activity and Structural Analogues of Guaianolide Derivatives from the Flower of Chrysanthemum coronarium L.
Lee, Kyung-Dong ; Park, Ki-Hun ; Kim, Hoon ; Kim, Jung-Ho ; Rim, Yo-Sup ; Yang, Min-Suk ;
Journal of Applied Biological Chemistry, volume 46, issue 1, 2003, Pages 29~32
Four bioactive compounds were isolated from dried flower of Chrysanthemum coronarium L. by the repeated silica gel column chromatography and recrystallization. Their structures were determined using various spectroscopic data and, when compared with the literature data, and were identified as: dihydrocumambrin A (1), cumambrin A (2), 8
-hydroxy-slov-3-en-6,12-olide (3), and tigloylcumambrin B (4). Isolated sesquiterpene lactones in C. coronarium L. were proved to be compounds of guaianolide derivatives. Compounds 3 and 4 reported for the first time in this plant. Compounds 2 and 4 showed strong cytotoxic activities (
, whereas 1 and 3 showed weak activities against human cancer cell lines such as A549, PC-3, HCT-15.
Antifungal Activity of Sporogen AO-1 and p-Hydroxybenzoic acid Isolated from Penicillium sp. AF5
Kang, Jae-Gon ; Hur, Jong-Hyun ; Yun, Bong-Sik ; Yoo, Ick-Dong ; Kang, Kyu-Young ;
Journal of Applied Biological Chemistry, volume 46, issue 1, 2003, Pages 33~37
An antagonistic fungus (AF5) was isolated from greenhouse soil. The fungus exhibited inhibitory activity against several phytopathogens in a dual culture on a potato dextrose agar medium, and was identified as Penicillium sp. based on its mycological characteristics. The antifungal compounds, sporogen AO-1 and p-hydroxybenzoic acid, were isolated from a culture broth of the Penicillium sp. AF5 and purified through solvent partition, silica gel column chromatography, and preparative TLC. Sporogen AO-1 showed strong activity either on mycelial growth (
= <40 ppm) of Phytophthora capsici, Pythium ultimum, and Rhizoctonia solani or zoospore germination of Phytophthora capsici. p-Hydroxybenzoic acid exhibited weak activity.