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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
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Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 46, Issue 4 - Dec 2003
Volume 46, Issue 3 - Sep 2003
Volume 46, Issue 2 - Jun 2003
Volume 46, Issue 1 - Mar 2003
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Hyperglycosylation of Aspergillus ficuum Endoinulinase Increases the Optimal Temperature and Thermal Stability
Kim, Hee-Un ; Park, Seoc-Kyu ; Uhm, Tai-Boong ; Chae, Keon-Sang ; Kim, Jong-Hwa ;
Journal of Applied Biological Chemistry, volume 46, issue 2, 2003, Pages 43~46
Purified Aspergillus ficuum endoinulinase (Afi-Endo) previously produced by a recombinant Saccharomyces cerevisiae was heterogeneous in its molecular weight, ranging from 84 to 130 kDa. When an N-linked endoglycosylase, Endo H, was treated to the heterogeneous Afi-Endo, the recombinant enzyme showed a single band of 66 kDa on SDS-PAGE, suggesting that the recombinant enzyme is hyperglycosylated. Hyperglycosylated enzyme had higher optimal temperature and thermal stability than Afi-Endo produced from A. ficuum. Specific activities of hyperglycosylated and deglycosylated Afi-Endo were equal. These results indicate that hyperglycosylation increases optimal temperature and thermal stability of Afi-Endo, but does not affect specific activity. Hyperglycosylated Afi-Endo has more advantages than Afi-Endo produced from A. fucuum for industrial applications.
Proteomic Analysis of Light Stress Response in Arabidopsis thaliana
Phee, Bong-Kwan ; Bhoo, Seong-Hee ;
Journal of Applied Biological Chemistry, volume 46, issue 2, 2003, Pages 47~51
Light stress responses of plants were studied by proteomic approach. Proteins extracted from Arabidopsis seedlings grown under low and high light conditions were separated by 2-dimensional polyacrylamide gel electrophoresis. Expressed protein profiles were analyzed by 2-dimensional gel analysis. Differently expressed protein spots were picked using spot handling workstation followed by MALDI-TOF mass analysis. Fourty-eight protein spots were analyzed by MALDI-TOF mass spectrometry, among which 7 and 17 proteins were down-and up-regulated, respectively. Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (nos. 1,2,3) was down-regulated, and antioxidant proteins (nos. 26, 38) and photosystem II-related oxygen-evolving proteins (no. 34) were up regulated by light stress. Chaperonin protein expressed with heat stress (no. 33) and jasmonate-inducible protein isolog (no. 22) involved in defense mechanism for various abiotic stresses were also up-regulated by light stress. All these proteins were highly induced with high light rather than under low light condition.
Constitutive Expression of Defense Genes in Transgenic Arabidopsis Overproducing Methyl Jasmonate
Jung, Choon-Kyun ; Lyou, Seoung-Hyun ; Koo, Yeon-Jong ; Song, Jong-Tae ; Choi, Yang-Do ; Cheong, Jong-Joo ;
Journal of Applied Biological Chemistry, volume 46, issue 2, 2003, Pages 52~57
Methyl jasmonate modulates diverse developmental processes and defense responses in plants, acting as an important cellular regulator. Formation of this volatile is catalyzed by a jasmonic acid carboxyl methyltransferase (JMT). Expression of various defense genes in transgenic Arabidopsis overexpressing the JMT gene, thus containing elevated level of endogenous methyl jasmonate, was examined. Constitutive expression of various defense genes including those for defensin, pathogenesis-related proteins, and oxidative stress-resistant enzymes was observed through Northern and dot blot analyses. The transgenic plants exhibited enhanced resistance to a non-host virulent bacterial pathogen, Pseudomonas syringae pv tomato DC3000. Taken together with previous reports, our data imply that genetic introduction of MeJA-producing gene could be used to achieve fortified resistance of plants against various pathogens.
Isolation and Characterization of Salt Tolerance Rhizobia from Acacia Root Nodules
Gal, Sang-Wan ; Choi, Young-Ju ;
Journal of Applied Biological Chemistry, volume 46, issue 2, 2003, Pages 58~62
Forty strains of Acacia rhizobia spp. were isolated from Acacia root nodules. Salt tolerance Acacia rhizobial strains which can grow at concentration of up to 1.4 M sodium chloride were isolated and characterized. In Acacia, both fast-growing and slow-growing rhizobia occur naturally, and fast-growing species are predominant throughout the isolates. The fast-growing Acacia rhizobia accumulate intracellular free glutamate in response to salt stress. Fast-growing rhizobial strains showed strong and weak resistance to streptomycin and gentamycin, respectively. By one-dimension SDS- polyacrylamide gel electrophoresis, distinct difference was observed between salt tolerant (fast-growers) and sensitive (slow-growers) strains. One or two large and three small plasmids with molecular weights about 15 kb, 5 kb, and 1.5 kb were isolated from fast-growing strains. This is the first study to report that Acacia rhizobia contain small plasmid of nitrogen fixing organism. These plasmids of Acacia rhizobia are very useful as genetic engineering tools.
Determination of Fungicide Mancozeb by a Bioassay Method Based on the Inhibition of Triphenyltetrazolium Chloride Reduction by Isolate Bacillus sp. CMB03
Shim, Jae-Han ; Shen, Jing-Yu ; Kim, Mi-Ra ; Lee, Chang-Joo ; Kim, In-Seon ; No, Sang-Myung ; Chi, Youn-Tae ;
Journal of Applied Biological Chemistry, volume 46, issue 2, 2003, Pages 63~66
A simple method for the determination of ethylene bisdithiocarbamate fungicide mancozeb was studied using a bioassay based on the inhibition of triphenyltetrazolium chloride (TTC) reduction by mancozeb-sensitive bacterial isolates. An isolate CMB03 showed the most sensitive response to mancozeb, giving
value of about 0.1
/ml, and based on 16S rRNA sequence analysis was named Bacillus sp. CMB03. Dose-dependent growth inhibition of CMB03 by mancozeb suggested that a biochemical system of CMB03 can be used as a bioassay method for the determination of mancozeb. TTC reduction to formazan, a red color product, by CMB03 was examined as biological reaction indicator responsive to mancozeb. When cells were incubated with or without mancozeb in the medium containing TTC and the absorbance of formazan was measured at 484 nm, dose-dependent decrease in absorbance of formazan was observed with increasing concentration of mancozeb tested, through which the inhibition calibration curve ranging from 0.01 to 1.0
/ml, giving 258.99 of slope value, 16.131 of y-intercept value and 0.9739 of mean
value, was built. The bioassay method showed approximately 70% recovery of mancozeb spiked in lettuce samples. Our method is suggested to be useful method for the determination of mancozeb in agricultural samples.
Human Brain GABA-T (
-aminobutyric acid transaminase) Inhibitory Alkaloids from Corydalis Tuber
Choi, Soo-Young ; Bang, Myun-Ho ; Lee, Eun-Jeong ; Kwon, Oh-Shin ; Kang, Tae-Cheon ; Lee, Youn-Hyung ; Rho, Yeong-Deok ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 46, issue 2, 2003, Pages 67~72
The alkaloid fraction obtained from 80% MeOH extracts of Corydalis tuber showed a significant inhibitory effect on brain GABA-transaminase (GABA-T). Each alkaloid fractions using
and ODS column chromatography were isolated in seven isoquinoline alkaloids. They were identified as R-(+)-stylopine, S-(-)-corydaline, R-(+)-canadine, R-(+)-tetrahydropalmatine, protopine, S-(+)-glaucine and dehydrocorydaline, based on the interpretation of several spectral data and in comparison with those of literatures. All they inhibited the activity of GABA-T.
Isolation of Pyrethrosin Derivatives from the Flower of Chrysanthemum coronarium L.
Lee, Kyung-Dong ; Park, Ki-Hun ; Ha, Tae-Joung ; Han, Hyo-Shim ; Yang, Min-Suk ;
Journal of Applied Biological Chemistry, volume 46, issue 2, 2003, Pages 76~79
From the chloroform extract of Chrysanthemum coronarium L. flowers, three sesquiterpene lactones were isolated by the repeated silica gel column chromatography and recrystallization. Their structures were determined using various spectroscopic data, and were identified as: pyrethrosin (1), 1-10-epipyrethrosin (2), and tulirinol (3), respectively. Compounds 1-3 are reported for the first time in this plant. The cytotoxic activity of compounds 1-3 showed strong activities against A547, PC-3 and HCT-15 human cancer cell lines with
values ranging from 1.5 to 3.5