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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
Journal Basic Information
Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 46, Issue 4 - Dec 2003
Volume 46, Issue 3 - Sep 2003
Volume 46, Issue 2 - Jun 2003
Volume 46, Issue 1 - Mar 2003
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Enzyme-Linked Immunosorbent Assays for the Neonicotinoid Insecticides
Kim, Hee-Joo ; Shelver, Weilin L. ; Keum, Young-Soo ; Hwang, Eul-Chul ; Li, Qing. X. ;
Journal of Applied Biological Chemistry, volume 46, issue 4, 2003, Pages 133~136
The neonicotinoids are a new class of insecticides acting as agonists at the postsynaptic nicotinic acetylcholine receptors. Enzyme-linked immunosorbent assays (ELISAs) are sensitive, selective and capable of cost effective and high throughput, which make them ideal for the determination of pesticides in a myriad of applications where these properties are required. ELISAs developed by different laboratories for the neonicotinoid insecticides can be an alternative method to analyze these insecticides in environmental samples and can overcome some of the shortcomings of conventional instrumental analysis. This mini review summarizes currently available ELISAs for the neonicotinoid insecticides imidacloprid, acetamiprid and thiamethoxam.
QSAR Analyses on the Cell Cytotoxicity of Some N-phenyl-O-phenylthionocarbamate Derivatives Using Comparative Molecular Field Analysis (CoMFA) Based on Different Alignment Approaches and Holographic Quantitative Structure-Activity Relationship (HQSAR)
Sung, Nack-Do ; Myung, Pyung-Keun ; Seong, Min-Gyu ; Yu, Seong-Jae ; Choi, Su-La ;
Journal of Applied Biological Chemistry, volume 46, issue 4, 2003, Pages 137~143
A series of 22 new N-phenyl-O-(4-methoxyphenyl)thionocarbamate derivatives were synthesized and examined in vitro for cytotoxicity on human skin cancer cell line, SK-MEL-28 cells, by MTT assay. 3D-QSAR/CoMFA and HQSAR models were developed to guide the rational design of novel analogs exhibiting improved biological property. Two models exhibiting excellent self-consistency (
>0.90) and predictability (
>0.50) were developed. For alignment, atom fit (
=0.526) and field fit (
=0.495) were applied to compare the predictability of each model in CoMFA. Quality of HQSAR (
=0.807) was slightly higher than that of CoMFA in terms of cross-validated predictive correlation coefficient,
values, but showed relatively poor predictability. Cytotoxicity (
) against SK-MEL-28 cells, based on CoMFA analysis results, exhibited a good correlation. Reliable results could be obtained using the two models for predicting in vitro cytotoxicity (
) against SK-MEL-28 cells of thionocabamate derivatives.
Molecular Cloning and Nucleotide Sequence of a Gene Encoding a Glycogen Debranching Enzyme in the Trehalose Operon from Brevibacterium helvolum
Kim, Chung-Ho ;
Journal of Applied Biological Chemistry, volume 46, issue 4, 2003, Pages 144~147
A gene designated bvtreX, located just upstream of bvtreYZ, was isolated from Brevibacterium helvolum (ATCC 11822). B. helvolum bvtreYZ genes encode maltooligosyltrehalose synthase (BvMTS) and maltooligosyltrehalose trehalohydrolase (BvMTH). Open reading frame of bvtreX is 2,265 bp long and encodes 754 amino acid residues. Estimated molecular weight of the polypeptide is 84,851 Da. Translation termination codon of the bvtreX gene overlaps the translation initiation codon of the bvtreY gene by four nucleotides. Deduced amino acid sequence of B. helvolum bvtreX revealed identity with glycogen debranching enzymes from Arthrobacter sp. Q36, Mycobacterium tuberculosis H37Rv, Corynebacterium glutamicum, Sulfolobus shibatae, Sulfolobus acidocaldarius, and Xanthomonas campestris. These results suggest B. helvolum bvtreX is a glycogen debranching enzyme gene, a component of a bvtreXYZ operon of B. helvolum.
Comparison of Soybean Varieties for Cyclitol Content in Soybean Curd Wastewaters
Lee, Yong-Kwon ; Park, Phun-Bum ; Park, Yong-In ; Chung , Nam-Hyun ;
Journal of Applied Biological Chemistry, volume 46, issue 4, 2003, Pages 148~151
D-chiro-Inositol and its methylated form, pinitol, are major cyclitols of soybeans and play important roles in seed maturation. Cyclitols are often used as dietary supplements for acute type II diabetes. Process optimization was performed by varying grinding times, soaking times, and coagulants for the isolation of cyclitols from soybean curd wastewater. The optimal condition was 4 min of grinding, 5 h of soaking.
was also employed as a coagulant. By using the optimal conditions, the highest amount of cyclitols was obtained from Shinpaldal among 12 varieties of Korean soybeans. Shinpaldal had about 24% more cyclitols than US No. 1 did.
Identification of Volatile Compounds in Structured Lipid of Safflower Oil using Electronic Nose and Solid Phase Microextraction Gas Chromatograph-Mass Spectrometry
Shin, Jung-Ah ; Lee, Jong-Ho ; Lee, Ki-Teak ;
Journal of Applied Biological Chemistry, volume 46, issue 4, 2003, Pages 152~155
Structured lipide were synthesized by esterification reaction safflower oil and conjugated linoleic acid (CLA) in shaking water bath at 200 rpm for 24 hr at
. Excessive free fatty acids were removed by deacidification with alkali refining. In this study, 26.45 mol% of CLA were incorporated into safflower oil. The major volatile compounds from safflower oil and SL-safflower oil were identified by electronic nose system and solid phase microextraction (SPME) method combined with gas chromatograph-mass spectrometry (GC-MS).
Chiral Diphosphites Derived from (L)-Tartaric Acid: New Effective Ligands for the Enantioselective Hydrogenation
Kyung, Suk-Hun ; Kim, Chi-Hyun ;
Journal of Applied Biological Chemistry, volume 46, issue 4, 2003, Pages 156~159
New chiral diphosphites derived from (L)-tartaric acid (1),1, 2-ethanediol and (S)-2,2'-dihydroxy-1,1'-binaphthyl [(S)-BINOL], were synthesized, and applied as ligands in metal-catalyzed asymmetric hydrogenation reactions. The phosphite derived from (L)-tartaric acid and (S)-BINOL provided moderate ee value for dimethyl itaconate, high ee value for
-acetamino methyl cinnamate in Rh-catalyzed hydrogenation.
Differences in the Thermal Degradation Products of Various Lignins from Arabidopsis thaliana L.
Hwangbo, Jun-Kwon ; Kim, Jae-Sung ; Kim, Jin-Hong ; Baek, Myung-Hwa ; Lee, Young-Keun ; Lewis, Norman G. ; Chung, Byung-Yeoup ;
Journal of Applied Biological Chemistry, volume 46, issue 4, 2003, Pages 160~164
Characterization of the thermal degradation products of various Arabidopsis lignins was performed by pyrolysis GC/MS. The lignin from a wild type showed high intensities of both guaiacyl and syringyl units and low intensity of p-hydroxyphenyl unit. Guaiacyl units mainly appeared in fah1-2 mutant, and syringyl units appeared in ferulate-5-hydrolylase overexpressed using the cinnamate-4-hydroxylase promoter. Thermal degradation patterns of various lignins from Arabidopsis plants are in good agreement with those obtained through alkaline nitrobenzene oxidation.
Prolyl Endopeptidase Inhibiting Principles from Raw Medicinal Materials
Lee, Jung-Han ; Paik, Young-Sook ;
Journal of Applied Biological Chemistry, volume 46, issue 4, 2003, Pages 165~168
Prolyl endopeptidase (PEP, EC 22.214.171.124) has been suggested to participate in learning and memory processes because it can degrade proline-containing neuropeptides. Yook-Mee-Tang, a traditional combination of raw medicinal materials, was tested for inhibitory activity against PEP. Specified fractions of six raw materials in Yook-Mee-Tang, i.e., Alisma orientale (rhizome), Cornus officinallis (fructus), Dioscorea batatas (rhizome), Paeonia suffruticosa (radicis cortex), Poria cocos (hoelen), and Rehmannia Glutinosa (radix) showed 16-100% PEP inhibitory activities at 8
Effect of the Fungicide Chlorothalonil on Microbial Activity and Nitrogen Dynamics in Soil Ecosystem
Rahman, Md. Mokhlesur ; Kim, Taek-Kyum ; Rhee, In-Koo ; Kim, Jang-Eok ;
Journal of Applied Biological Chemistry, volume 46, issue 4, 2003, Pages 169~173
Impact of the fungicide chlorothalonil on soil ecological processes was evaluated through substrate-induced respiration (SIR) of soil, soil enzyme activity, and nitrogen dynamics. Chlorothalonil application at tenfold field rate decreased SIR (6-20%) and dehydrogenase activity (45-61%) more prominently in the unamended than amended soil, whereas had no influence on urease activity and
content initially decreased in both unamended and amended soils treated at tenfold field rate. In both soils, chlorothalonil had no significant inhibitory impact on net mineralization rate, whereas net nitrification rate was initially decreased by the higher rate of chlorothalonil application. A rapid decrease in chlorothalonil was observed at the higher application rate in both soils, and their half-lives varied from 9.1 to 9.5 days. Fungicide treatment at the recommended field rate had no inhibitory impact on soil microbial activity and nitrogen dynamics. Conclusively, effect of chlorothalonil on cycling processes of soil nutrient was not remarkable, but its impact on soil microbial activity depended on the addition of organic amendment to the soil.
-Irradiation on the Physicochemical Properties of Orange Pectinesterase
Jang, Jae-Won ; Song, Kyung-Bin ;
Journal of Applied Biological Chemistry, volume 46, issue 4, 2003, Pages 177~179