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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
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Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 47, Issue 4 - Dec 2004
Volume 47, Issue 3 - Sep 2004
Volume 47, Issue 2 - Jun 2004
Volume 47, Issue 1 - Mar 2004
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Advances in Studies on Bioactivity of Hovenia dulcis
Xu, Bao-Jun ; Deng, Yu-Qiu ; Sung, Chang-Keun ;
Journal of Applied Biological Chemistry, volume 47, issue 1, 2004, Pages 1~5
Hovenia dulcis is one of the earliest medicinal plants used in traditional Chinese medicine. It has many profound pharmacological actions including alcohol detoxification activity. This article summarizes recent progress on the pharmacological and biochemical studies of Hovenia dulcis.
Phospholipase C and Sphingomyelinase Assays for High Throughput Screening
Do, Ernest U. ; Karge, William III ; Kim, Su-Il ;
Journal of Applied Biological Chemistry, volume 47, issue 1, 2004, Pages 6~10
Assay miniaturization is essential for meeting the cost and throughput goals of screening laboratories, and 384-well microtiter plate format is a convenient platform to increase efficiency and decrease cost of the assays. Phospholipid FlashPlate is a homogeneous platform ideally suited to high throughput screening and allows for the quantitative and qualitative determinations of the activities of various enzymes that act on membrane-bound substrates, without the need for organic extraction. Most of the hydrolysis of the labeled substrates occurred during the first 4 h of incubation. The procedure allows quantification of sphingomyelin over a range of 0.01 to 3.0 pmol. This application demonstrates the successful miniaturization of 96-well and 384-well Phospholipid FlashPlates for phospholipase
isozymes and sphingomyelinase from Bacillus cereus assays with no transfer, separation, and wash steps.
The Cell Wall Characterization at Immature and Mature Stages of Arabidopsis thaliana L.
Kim, Jin-Hong ; Kim, Jae-Sung ; Wi, Seung-Gon ; Mun, Sung-Phil ; Chung, Byung-Yeoup ;
Journal of Applied Biological Chemistry, volume 47, issue 1, 2004, Pages 11~14
Glucose contents of Arabidopsis at immature and mature stages were 22.06 and 32.80%, and those of xylose were 5.23 and 11.87%, respectively. Mature-staged Arabidopsis gave high yields of vanillin [
of cell wall residue (CWR)] and syringaldehyde (
of CWR) as G and S units of lignin, respectively, by alkaline nitrobenzene oxidation (NBO), while p-hydorxybenzaldehyde (
of CWR) as H unit was predominant at immature stage with low amounts of G (
of CWR) and S units. NBO results revealed deposition of monolignols is in the order of H, G, and S units as confirmed by analytical pyrolysis GC/MS.
Isolation of Rice Putative STY Kinase Gene Induced by both Abiotic and Biotic Stress
Jeong, Mi-Jeong ; Kim, Beom-Gi ; Lee, Jin-Ohk ; Kim, Sun-Young ; Park, Soo-Chul ; Go, Sung-Joo ;
Journal of Applied Biological Chemistry, volume 47, issue 1, 2004, Pages 15~19
We isolated a novel protein kinase gene from Oryza sativa, and observed that expression of the gene is induced in respond not only to cold stress but also to drought and wounding stresses. Amino acid sequence deduced from the gene showed homology with that of a possible dual-specificity Ser/Thr/Tyr (STY) kinase. Time course expression profiling showed that mRNA expression of this gene reached maximal level at the early stage of stress by cold, drought, or wounding treatments. Our data suggest that the putative STY kinase plays a key role in upstream of abiotic and biotic signal transduction pathways and may also be involved in convergent signal pathways of abiotic and biotic stresses. In addition, we isolated several stress-related genes encoding proteins that interact with the putative STY kinase using the yeast two-hybrid screening system.
Selection of Probiotic Yeasts from Soil, Characterization and Application for Feed Additives
Kim, So-Young ; Kim, Hong ; Chae, Hee-Jeong ;
Journal of Applied Biological Chemistry, volume 47, issue 1, 2004, Pages 20~26
Yeast strains were isolated from soil and characterized for the development of functional probiotics. Most strains selected showed high resistance against acids and were very stable in a broad range of pH, some showing 100% survival at pH 2.5. Growth of the strains was not affected by the presence of bile acid, pathogenic Escherichia coli, and antibiotics such as tetracycline, kanamycin, streptomycin, and ampicillin. Acidogenic capability test showed all strains produced acids. Hydrolytic activities of amylase, protease, lipase, and cellulase to decompose various organic compounds were analyzed. All strains were found to be Gram-negative, round-type, and non-kinetic, with yellow or white color. Results of field tests on pigs and cows revealed some selected microbes were very effective in increasing body rate, decreasing production time, somatic cell number, and ammonia gas production, and providing higher milk quality.
In vitro Antioxidant Activity of 3,5-Dicaffeoylquinic Acid Isolated from Aster scaber Thunb.
Lee, Seung-Eun ; Chung, Tae-Yung ; Seong, Nak-Sul ; Bang, Jin-Ki ;
Journal of Applied Biological Chemistry, volume 47, issue 1, 2004, Pages 27~30
Antioxidant characteristics of 3,5-dicaffeoylquinic acid isolated from Aster scaber Thunb. were investigated using several oxidation systems. Compared with several phenolic and antioxidant compounds, 3,5-dicaffeoylqunic acid exhibited effective radical scavenging activities on superoxide anion and hydroxyl radical, and inhibited
human low density lipoprotein oxidation at
, showing the most effective activity among tested phenolic compounds and
-tocopherol. Antixodative activities on linoleic acid autoxidation and
Effect of Gamma-Irradiation on Microbiological Change of Fresh Lettuce Juice During Storage
Lee, Yong-Woo ; Song, Kyung-Bin ;
Journal of Applied Biological Chemistry, volume 47, issue 1, 2004, Pages 31~34
To elucidate the effect of gamma-irradiation on the microbial change in lettuce juice during storage, fresh lettuce juice was prepared and irradiated at 0, 0.1, 0.3, 0.5, 0.7, and 1.0 kGy. D-values for total bacteria, mold and yeast, Salmonella, and Pseudomonas in lettuce juice were 0.085, 0.084, 0.107, and 0.098 kGy, respectively. Viable microorganisms were eliminated completely at 0.5 kGy. Gamma-irradiation also reduced total viable microorganisms during storage.
Effect of Vacuum Packaging on the Microbiological Profile of Chilled Chicken during Storage
Kim, Ji-Youn ; Song, Kyung-Bin ;
Journal of Applied Biological Chemistry, volume 47, issue 1, 2004, Pages 35~37
Effects of vacuum packaging on populations of total bacteria, Pseudomonas, mold and yeast, and Salmonella in chicken during storage were determined using chicken breasts vacuum-packaged and stored at
for 15 days. Vacuum packaging effectively retarded microbial growth and maintained chicken breast quality during chilling temperature storage.
-endosulfan by Anabaena sp. PCC 7120
Park, Byeoung-Soo ; Lee, Sung-Eun ;
Journal of Applied Biological Chemistry, volume 47, issue 1, 2004, Pages 38~41
The blue-green algae, Anabaena sp. PCC 7120 degraded
-endosulfan primarily into endosulfan diol (endodiol), and minor amounts of endosulfan hydroxyether, endosulfan lactone, and endosulfan sulfate were determined in the pure culture medium. The principal biotransformation product endodiol is much less toxic metabolite to fish and higher animals when compared to the parent compound
-endosulfan. Among the three minor metabolites, endosulfan hydroxyether, endosulfan lactone, and endosulfan sulfate, endosulfan sulfate has similar to endosulfan in harmful properties in environments. Endodiol production was dependent on the medium pH. However, the production of the hydroxylated metabolites was related to the some oxidative reaction in the algal cells. This study shows that the algal species may contribute in the detoxification of
-endosulfan in the environment.