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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
Journal Basic Information
Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 47, Issue 4 - Dec 2004
Volume 47, Issue 3 - Sep 2004
Volume 47, Issue 2 - Jun 2004
Volume 47, Issue 1 - Mar 2004
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Inhibitory Effects on Nitric Oxide Synthesis by Flavors
Choi, Won-Sik ; Park, Byeoung-Soo ; Lee, Sung-Eun ; Park, Kyu-Jong ; Kim, Soo-Yeon ; Lee, Young-Haeng ;
Journal of Applied Biological Chemistry, volume 47, issue 2, 2004, Pages 51~55
This study was undertaken to assess flavors or fragrances to modulate the expression of iNOS in thioglycollate-elicited murine peritoneal macrophages and murine macrophage cell line, RAW264.7 cells. Stimulation of the peritoneal macrophages and RAW264.7 cells with
and lipopolysaccharide (LPS) resulted in increased production of NO in the medium. p-Benzoquinone
had potent inhibitory effect on NO production among the tested volatile compounds. p-Cymene
, and benzaldehyde
showed moderate inhibitory effects on NO production, while the tested monoterpenes, alkyl benzene, t-anethole, 4-t-butylamide, camphor, geraniol and 2,4-hexadienal did not exhibit any effects on NO production. The inhibitory mode of activity of benzoquinone on NO production was the suppression of the expression of iNOS gene.
Malformin A1 Stimulates the Ethylene Production in Primary Roots of Maize (Zea mays L.)
Oh, Seung-Eun ; Hong, Sung-Hyun ; Kim, Kun-Woo ; Kim, Soon-Young ;
Journal of Applied Biological Chemistry, volume 47, issue 2, 2004, Pages 56~59
The purified malformin A1 stimulated ethylene production in the primary root segments of maize. And this stimulatory effect of malformin A1 was significant in the presence of 0.1 mM IAA. Especially, 10 uM malformin A1 increased the ethylene production about 60% of control. Further, malformin A1 stimulated the activity of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO), which is the key enzyme in the ethylene production pathway. And another enzyme in the ethylene production pathway such as ACC synthase (ACS) was not affected by the malformin A1. Therefore, the increased ethylene production by malformin A1 might be due to the increased in vivo ACO activity in corn roots.
Production of Microbial Biopolymer, Poly(
-glutamic acid) by Bacillus subtilis BS 62
Choi, Seong-Hyun ; Park, Jong-Soo ; Whang, Kyung-Sook ; Yoon, Min-Ho ; Choi, Woo-Young ;
Journal of Applied Biological Chemistry, volume 47, issue 2, 2004, Pages 60~64
Bacterium BS 62, identified as Bacillus subtilis in our previous work, produced poly(
-glutamic acid) (PGA) in medium composed of glucose, L-glutamic acid and inorganic salts. Cell growth, sporulation and polymer production were monitored during cultivations. Glucose was a better source of carbon than citric acid for PGA production. Both maximum amount of PGA production (19 g per liter culture fluid) and maximum spore formation were achieved at 96 h. HPLC analysis revealed that D- and L-glutamic acid composition ratio of PGA produced by B. subtilis BS 62 was 60/40 from 48 to 120 h cultivation. Weight-average molecular weight of PGA was estimated to be 624 kDa through gel permeation chromatography.
Mobilization of Iron into Cell from Ambient Particulate Matter and Its Possible Participations to DNA Single Strand Break
Song, Hee-Sang ; Sung, Suk-Hee ; Jin, Young-Woo ; Kim, Chong-Soon ; Kim, Yoon-Shin ; Bang, Won-Gi ; Chung, Nam-Hyun ;
Journal of Applied Biological Chemistry, volume 47, issue 2, 2004, Pages 65~70
Ambient particulate matter (PM) contains various transition metals. Ferritin level of A549 cells treated with PM increased up to about six-fold of untreated control cells. To compare extents of DNA damaging capacities of Fe and other metals, the induction of DNA single strand break (SSB) was measured in the presence of hydrogen peroxide and each metals. Extents of DNA SSB with iron(II), copper(II), and copper(I) were higher than those of nickel(II), chromium(III), and manganese(II). All these results suggested that Fe and other metals in ambient PM are easily mobilized into lung cells upon inhalation to cause oxidative damage and that extent of the damage cannot be assessed by only amount of metals but by both amount of and damage extent by metals.
Ecology of Vesicular-Arbuscular Mycorrhizal (VAM) Fungi in Coastal Areas of India
Selvaraj, Thangaswamy ; Kim, Hoon ;
Journal of Applied Biological Chemistry, volume 47, issue 2, 2004, Pages 71~76
A survey was undertaken in three coastal saline soil sites of the southern penninsular tip of India for the occurrence and quantification of vesicular-arbuscular mycorrhizal (VAM) fungi. Sixteen VAM fungal species were isolated from rhizosphere soils of 15 plant species. To confirm infection efficiency, woody legume Prosopis juliflora present commonly in the three sites was inoculated in field with selected VAM fungal spores. Glomus macrocarpum was found as the most efficient VAM species. VAM fungi enabled all vegetations, Prosopis juliflora in particulr, to survive in the disturbed saline-stressed ecosystem.
Quinone Methide Triterpenes from Tripterygium regelii
Lee, Byong-Won ; Seo, Woo-Duck ; Gal, Sang-Wan ; Yang, Min-Suk ; Park, Ki-Hun ;
Journal of Applied Biological Chemistry, volume 47, issue 2, 2004, Pages 77~80
Chloroform extracts of Tripterygium regelii dried root bark afforded three cytotoxic quinone methides, which were identified as pristimerine (1), celastrol (2), and
-hydroxy tingenone (3) on the basis of chemical and spectroscopic studies. Compounds 1-3 showed significant cytotoxic
against A549 and SK-OV3 human cancer cell lines, and antibacterial activities against Gram-positive bacteria. 1 was isolated from this plant for the first time.
Synthesis of Optically Active
-symmetric Chiral Building Blocks in both Enantiomeric Pairs
Koh, Dong-Soo ;
Journal of Applied Biological Chemistry, volume 47, issue 2, 2004, Pages 81~85
An enantiomeric pair of
-symmetric (R, R)- and (S, S)-enonates (6,12) was synthesized from D-(-)- and L-(+)-arabinose respectively as optically active starting materials. Chain extended sugar enonate 3 was obtained by Wittig reaction of aldehyde group of D-(-)-arabinose without protection. After Wittig reaction, tetraol of sugar moiety was protected with dimethoxypropane. Selectively partial deprotection at terminal hydroxyl group was made with Dowex resin. Diol cleavage followed by Wittig reaction gave chiral
-symmetric (R, R)-enonate 6. The same (S, S)-enonate (12) with opposite configurations was prepared from L-(+)-arabinose.
Biosorption of Hexavalent Chromium by Pachymeniopsis sp. Dead Cells
Kim, Yong-Mu ; Yang, Jae-E ; Rhee, Hae-Ik ;
Journal of Applied Biological Chemistry, volume 47, issue 2, 2004, Pages 86~90
Selectivity and adsorption mechanism of red marine algae Pachymeniopsis sp. were batch-tested for heavy metals. Biosorbent showed higher selectivity for chromium than cadmium and manganese. Chromium adsorption by Pachymeniopsis sp. dead cell powder followed Langmuir-type isotherm, with maximum adsorption capacity being 225 mg/g. Chromium sorption isotherm of Pachymeniopsis sp. dead cell powder was similar to that of anion exchange resin and sorption mechanism was proved to be through anionic exchange of biosorbent. Desorption of chromate from biosorbent was more effective at higher pH. FTIR spectra showed cell wall components of Pachymeniopsis sp. were different from those of alginic acid, agarose, and
-carrageenan. Results demonstrated Pachymeniopsis sp. dead cell powder had strong potential as biosorbent for treatment of wastewater containing hexavalent chromium.
Anti-Helicobacter pylori Activity of Methanol Extracts from Korean Native Plant Species in Jeju Island
Lee, Hyun-Kyung ; Lee, Haeng-Byoung ; Kim, Cheol-Soo ; Ahn, Young-Joon ;
Journal of Applied Biological Chemistry, volume 47, issue 2, 2004, Pages 91~96
The antibacterial activity of methanol extracts from 124 Korean native plant species in Jeju Island toward Helicobacter pylori ATCC 43504 was examined using a paper disk diffusion bioassay and compared with those of the widely used antibiotics amoxicillin, metronidazole, and tetracycline. At
, potent antibacterial activity (zone diameter,
) was obtained from methanol extracts from the leaves of Elaeocarpus sylvestris var. ellipticus, Juglans sinensis, Ligularia fisheri, Magnolia sieboldii, Platycarya strobilacea, Rhus succedanea, Rhus trichocarpa, Sapium japonicum, Saururus chinensis, and Styrax japonica. At
, L. fischeri and S. chinensis leaf extracts were more effective toward H. pylori than metronidazole but less active than amoxicillin and tetracycline. These plants described merit further study as potential antibacterial agents for H. pylori.
Growth-Inhibiting Effects of Various Valley Potato (Solanum tuberosum L.) Varieties and Breeding Clones toward Human Intestinal Bacteria
Lim, Mi-Youn ; Kim, Young-Mi ; Lim, Hak-Tae ; Kim, Moo-Key ; Lee, Hoi-Seon ;
Journal of Applied Biological Chemistry, volume 47, issue 2, 2004, Pages 97~101
Ethanol-water extracts 29 valley potato varieties and breeding lines were tested in vitro for their growth-inhibiting activities against Bifidobacterium bifidum, B. breve, B. longum, Clostridium perfringens, Escherichia coli, and Lactobacillus casei using paper disk agar diffusion under anaerobic conditions. All potato varieties tested did not inhibit growths of B. bifidum, B. breve, B. longum, and L. casei. Extracts of Bora Valley, Gogu Valley, Golden Valley, Juice Valley, Purple Valley, Summer Valley, Taedong Valley, and Winter Valley showed moderate growth inhibition (++) against C. perfringens. Against E. coli, extracts of Juice Valley and Winter Valley showed moderate growth inhibition, and Bora Valley, Gogu Valley, Golden Valley, Purple Valley, R-chip Valley, and Summer Valley had weak (+) inhibitory effects. These results may be indications of at least one of the positive intestinal effects of potato varieties tested.
PCR Detection of Listeria monocytogenes Using Specific Virulence Factor Genes
Kim, Hyun-Joong ; Jung, Sang-Hoon ; Park, Jong-Hyun ; Ji, Geun-Eog ; Kim, Hae-Yeong ;
Journal of Applied Biological Chemistry, volume 47, issue 2, 2004, Pages 102~105
To investigate the novel specific genes of Listeria monocytogenes, six genes related with virulence factor of L. monocytogenes were selected from database. Primer sets were constructed from the selected six genes to evaluate the specificity of L. monocytogenes with other Listeria species. The primers were tested with 12 strains of Listeria species: L. monocytogenes (7 strains), L. welshimeri, L. innocua, L. seeligeri, L. ivanovii, and L. grayi. All primer sets showed specificity to L. monocytogenes but not with other Listeria species. These genes could be used as target genes for the detection of L. monocytogenes.
Effect of Vesicular-Arbuscular Mycorrhizal (VAM) Fungi on Tolerance of Industrial Effluent Treatment in Datura metal
Selvaraj, Thangaswamy ; Kim, Hoon ;
Journal of Applied Biological Chemistry, volume 47, issue 2, 2004, Pages 106~109
Effluent collected from industry in India was acidic, pH 6.4, and Zn level was higher than other heavy metals such as Cu, Ni, and Pb. It was treated to experimental plant, Datura metal, under potted condition. Plant was inoculated with four types of VAM fungal spores. Results revealed Glomus geosporum was most efficient effluent-tolerant VAM fungi in inducing plant growth compared with Gl. fasciculatum, Gl. mosseae, and Sclerocystis sinuosa. Gl. geosporum gave higher level of percentage root colonization (92%), number of VAM spores (445/100 g of soil), dry matter content (1,421 mg/plant) and phosphorus uptake in roots and shoots (3.8 and 7.2 mg/g dry sample, respectively).