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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
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Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 47, Issue 4 - Dec 2004
Volume 47, Issue 3 - Sep 2004
Volume 47, Issue 2 - Jun 2004
Volume 47, Issue 1 - Mar 2004
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Promoter Analysis of the NTR1 Gene of Brassica campestris L. ssp. pekinensis in Transgenic Tobaccos
Koo, Yeon-Jong ; Song, Jong-Tae ; Choi, Yang-Do ;
Journal of Applied Biological Chemistry, volume 47, issue 3, 2004, Pages 119~124
The NTR1 gene is specifically expressed in the floral nectaries of Brassica campestris L. ssp. pekinensis, which encodes an S-adenosyl-L-methionine:jasmonic acid methyltransferase. To understand the regulation mechanism of the NTR1 gene during nectary development, promoter 5'-deletion series were constructed by fusing translationally to a
-glucuronidase (GUS) reporter gene. Histochemical GUS staining and quantitative fluorometric analysis with the transgenic tobaccos indicated that regions spanning about -4.4 kb to -2.1 kb and -1.0 kb to -0.5 kb from the translation initiation site of NTR1, were essential for reasonable expression of GUS reporter gene. Transgenic tobaccos transformed with encompassing either -1.7 kb or -0.5 kb region of the NTR1 promoter caused the basal level of GUS activity. These results suggest that sequences of NTR1 promoter flanking about -4.4 kb to -2.1 kb and about -1.0 kb to -0.5 kb are important as a strong activator and for the nectary-specific expression during nectary development, respectively.
Expression of recombinant GFP in Agrobacterium-inoculated leaf-disks of Nicotiana tabacum var. xanthi
Hong, Seong-Hyun ; Kim, Kyung-Il ; Chung, Ho-Yong ; Lee, Youn-Hyung ; Chung, In-Sik ;
Journal of Applied Biological Chemistry, volume 47, issue 3, 2004, Pages 125~128
Recombinant GFP was transiently expressed in Agrobacterium-inoculated leaf-disks of Nicotiana tabacum var. xanthi with a molecular size of 28 kDa. Expression of GFP from a replicating vector based on tomato golden mosaic virus (TGMV) was 454% higher at the protein level than from a control vector of a non-replicating construct. The TGMV-based vector is a potentially useful agent for transient expression of transgenes in a plant cell system.
Enhanced Disease-resistance of Transgenic Arabidopsis Over-expressing the Defensin Gene PDF1.1
Kim, Eun-Deok ; Lyou, Seoung-Hyun ; Jung, Choon-Kyun ; Choi, Yang-Do ; Cheong, Jong-Joo ;
Journal of Applied Biological Chemistry, volume 47, issue 3, 2004, Pages 129~132
Defensins are antifungal peptides contributing to innate immunity in plants and animals. In this study, we characterized an Arabidopsis defensin gene PDF1.1 in detail. Northern blot analysis showed maximal accumulation of PDF1.1 gene transcripts 8 h after methyl jasmonate treatment on rosette leaves. Expression of the gene was induced by methyl jasmonate and ethylene treatments, exhibiting synergistic effect between the two volatile phytohormones. The gene was fused to CaMV35S promoter and transformed into Arabidopsis to generate transgenic plants over-expressing the gene. Successful transformants were selected on antibiotics-containing medium. Lines integrated with single copy of the transgene were further selected by genomic Southern blot analysis. The transgenic plants exhibited enhanced resistance to fungal pathogen Alternaria brassicicola, illustrating jasmonate-dependent defense response.
Expression of Thermotoga maritima 4-
-Glucanotransferase Gene in E. coli and Characterization of the Recombinant Enzyme
Kim, Kwang-Yup ; Kim, Chung-Ho ;
Journal of Applied Biological Chemistry, volume 47, issue 3, 2004, Pages 133~136
A gene encoding 4-
-GTase) was isolated from hyperthermophilic microorganism, Thermotoga maritima by polymerase chain reaction. Open reading frame (ORF) of 4-
-GTase gene is 1,326 bp long and encodes 441 amino acid residues. Estimated molecular weight of the polypeptide is 51,840 Da. To analyze enzymatic activity and biochemical properties of 4-
-GTase, ORF of 4-
-GTase gene was introduced into E. coli expression vector to produce pRBTmGT, and overexpressed in E. coli BL21. The purified recombinant 4-
-GTase showed highest activity at
, and pH 6.0-7.0, and was stable below
. Protein concentration of purified enzyme was 2.6 mg/ml with specific activity of 770 U/mg protein on amylose. Polysaccharides, such as soluble starch, amylose and amylopectin, and maltooligosaccharides longer than maltose could be effective maltosyl group donors for 4-
-GTase. All maltooligosaccharides were able to act as acceptor molecules in 4-
-GTase-mediated transfer of glucan segments from amylose.
Analysis of Aerobic and Culturable Bacterial Community Structures in Earthworm (Eisenia fetida) Intestine
Kim, Hyun-Jung ; Shin, Kwang-Hee ; Cha, Chang-Jun ; Hur, Hor-Gil ;
Journal of Applied Biological Chemistry, volume 47, issue 3, 2004, Pages 137~142
Aerobic intestinal bacterial community structure of earthworm, Eisenia fetida, was investigated based on 16S rDNA analysis. Ninety-one different colonies grown on Brain Heart Infusion medium were randomly isolated under aerobic condition. Based on partial sequence analysis of PCR-amplified 16S rDNA for strains, earthworm intestinal aerobic bacteria (EIAB) were divided into 12 groups, and each group was further divided into subgroups. Groups included 6% Aeromonas, 3% Agromyces, 31% Bacillus, 1% Bosea, 6% Gordonia, 6% Klebsiella, 7% Microbacterium, 2% Nocardia, 10% Pseudomonas, 19% Rhodococcus, 2% Tsukamurella, and 7% Streptomyces, with Bacillus being dominant group. Because earthworm plays key role in improving physical and chemical properties of soil, study on micro-intestinal ecology and community structure would provide valuable information.
Chemical Characterization and Bioavailability of Cadmium in Artificially and Naturally Contaminated Soils
Ok, Yong-Sik ; Lee, Han-Na ; Jung, Jin-Ho ; Song, Hee-Sang ; Chung, Nam-Hyun ; Lim, Soo-Kil ; Kim, Jeong-Gyu ;
Journal of Applied Biological Chemistry, volume 47, issue 3, 2004, Pages 143~146
Total concentration of cadmium in soil is often a poor indicator of cadmium availability to plants. In some Korean agricultural lands, cadmium concentration is steadily increasing due to the agricultural activities. But, it is still unknown whether this will lead to a concomitant increase of cadmium in plants grown on the soils. The objective of this research was to compare the cadmium availability of plants growing on the two contrasting soils with similar total cadmium concentration. The sequential extraction revealed that most of cadmium existed as immobile form (non-available fraction) in the naturally contaminated soil, while about 70% of cadmium existed as mobile form (available fraction) in the artificially contaminated soil. Spiking methods varying cadmium concentration, soil to solution ratio, and cadmium source had no effects on the 0.1 N HCl-extractable concentration of cadmium in soils. Aging of the spiked soils for 60 days also showed no difference in cadmium concentration in the artificially contaminated soil. To investigate the cadmium bioavailability by plant, Artemisia princeps var. orientalis was grown in the artificially and naturally contaminated soils and cadmium concentration was determined in the plant. Data showed that plant accumulated about five times higher concentrations of cadmium in the artificially contaminated soil as
than that in the field contaminated soil as
in the shoot. The overall result revealed that not the total concentration but the chemical form influenced the cadmium availability to plant.
Analysis of the Anaerobic Bacterial Community in the Earthworm (Eisenia fetida) Intestine
Shin, Kwang-Hee ; Yi, H. ; Chun, Jong-Sik ; Cha, Chang-Jun ; Kim, In-Seon ; Hur, Hor-Gil ;
Journal of Applied Biological Chemistry, volume 47, issue 3, 2004, Pages 147~152
Intestinal microbial community structure of earthworm Eisenia fetida was investigated based on 16S rDNA analysis. One hundred different colonies grown on Brain Heart Infusion medium were randomly isolated. Through partial sequence analysis of PCR-amplified 16S rDNA, earthworm intestinal bacteria (EIB) were divided into eight groups, which were further divided into subgroups. Groups EIB 2, EIB 3, EIB 4, EIB 5, EIB 6, EIB 7-1, and EIB 8 showed over 97% similarities to Clostridium bifermentans, C. butyricum, C. glycolicum, C. celerecrescens, C. lituseburense, Staphylococcus epidermidis, and Propionibacterium acnes, respectively. Group EIB 1 consisting of six subgroups, showed unique pyretic line, found to be most closely related to C. subterminale with 90-95% similarity. Subgroup EIB 7-2 showed 93% similarity to S. epidermidis. Among 100 strains, intestinal microbial community consisted of 49, 13, 13, 5, 4, 2, 11, and 3% EIB 1, EIB 2, EIB 3, EIB 4, EIB 5, EIB 6, EIB 7 and EIB 8, respectively, indications that group EIB 1 was dominant bacterial group in earthworm intestinal bacterial community. Considering earthworm plays key role in improving physical and chemical properties of soil, this study provides valuable information on bacterial community structure of intestine of these ecologically important organisms.
Expression of Thermotoga maritima Endo-
-1,4-xylanase Gene in E. coli and Characterization of the Recombinant Enzyme
Yoon, Hyang-Sik ; Han, Nam-Soo ; Kim, Chung-Ho ;
Journal of Applied Biological Chemistry, volume 47, issue 3, 2004, Pages 157~160
Gene encoding endo-1,4-
-xylanase (EC 184.108.40.206) was isolated from hyperthermophilic microorganism, Thermotoga maritima by polymerase chain reaction. Open reading frame (ORF) of xylanase gene is 3,180 bp long and encodes 1,059 amino acid residues. Estimated molecular weight of polypeptide is 119,642 Da. To analyze enzymatic activity and biochemical properties of xylanase, ORF of xylanase gene was introduced into E. coli expression vector, and overexpressed in E. coli BL21. Purified recombinant xylanase showed highest activity at
, and pH 6.0, and was stable below
. D-xyloseand xylooligosacharide-producing activities of purified recombinant xylanase was tested using oatspelts arabinoxylan and birchwood xylan as substrates. Enzyme activity of recombinant enzyme was 170 U/ml on oatspelts arabinoxylan.