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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
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Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 47, Issue 4 - Dec 2004
Volume 47, Issue 3 - Sep 2004
Volume 47, Issue 2 - Jun 2004
Volume 47, Issue 1 - Mar 2004
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Selenocompounds-induced Bimodal Functions as Measured for Oxidoreductive Potentials in Brassica campestris cv. Chibu
Kang, Han-Chul ; Kim, Jung-Bong ; Kim, Young-Sang ; Suh, Seok-Cheol ;
Journal of Applied Biological Chemistry, volume 47, issue 4, 2004, Pages 167~172
Bimodal effect of selenium which has been well known in animal was examined using cabbage grown with various seleniums. Higher concentration (
) of selenium dioxide, selenium selenite, and selenium selenate inhibited the growth of cabbage, whereas organic seleniums, selenomethionine and selenocystine, did not considerably influenced the growth. DPPH radical scavenging activity gradually decreased at lower concentration (below
) of inorganic seleniums and the activities increased at higher contents of the seleniums. Catalase activity began to markedly increase from about
of the seleniums. Activities of glutathion peroxidase and glutathion reductase only increased at lower concentration of seleniums and thereafter decreased. Reduced-glutathion concentration was slightly increased in response to the seleniums. Superoxide dismutase activity decreased at lower concentration of seleniums and markedly increased at higher concentration. Collectively, the activities of oxidoreductive enzymes which are mainly involved in the antioxidant reaction were significantly changed in response to the applied seleniums. Higher absorption of inorganic seleniums appears to act as a stress factor but appropriate subjection in plant can be involved in the reduction of stress.
Classification and Expression Analysis of Cytochrome P450 Genes from Soybean
Kim, Bong-Gyu ; Ko, Jae-Hyeong ; Hur, Hor-Gil ; Ahn, Joong-Hoon ;
Journal of Applied Biological Chemistry, volume 47, issue 4, 2004, Pages 173~177
Cytochrome P450 monooxygenases in plants are involved in various metabolic pathways. Synthetic pathways for phenylpropanoid, fatty acid, terpenoid and various plant hormones are only parts in which P450s are involved. With vast information available from various plant genome projects, many P450 sequences from several plants can be accessed. However, only few of their functions are known. Based on P450 classification system, eight-two soybean P450 genes were classified and their expression patterns in different tissues and upon UV-B irradiation analyzed by RT-PCR. In most soybean P450 genes, its expression pattern is correlated with P450 clan to which individual P450 belongs. It would be a first step to understand biological functions of individual P450s from soybean.
Prolyl Endopeptidase Inhibitory Activity of Omega-3 Polyunsaturated Fatty Acids
Jang, Hyun-Jung ; Paik, Young-Sook ;
Journal of Applied Biological Chemistry, volume 47, issue 4, 2004, Pages 178~180
Prolyl endopeptidase (PEP, EC 220.127.116.11), suggested to participate in learning and memory processes, has been reported to be abundant in brains of amnestic patients. Therefore, compounds possessing PEP inhibitory activity are expected to control memory loss and neuropathological disorders. Upon examination for PEP inhibition from dietary sources,
polyunsaturated fatty acids were chosen, because they are essential fatty acids for normal brain and their deficiencies are linked to decreased memory and mental abilities. Docosahexaenoic acid [22:6
] and eicosapentaenoic acid [20:5
] showed similar PEP inhibitory activity with
values of 21.8 (
, respectively. Diet high in essential
polyunsaturated fatty acids may help decrease age-related memory loss.
Phylogenetic Analysis of Korean Mugworts Based on Internal Transcribed Spacer Sequences of Ribosomal DNA
Nam, Hee-Young ; Kim, Bo-Ra ; Chang, Yung-Jin ;
Journal of Applied Biological Chemistry, volume 47, issue 4, 2004, Pages 181~184
Internal transcribed spacer (ITS), located between nuclear 18S and 28S ribosomal DNA genes, has been used for constructing phylogenic trees and determining taxonomic identity. In this work, ITS analysis was used for phylogenetic placements of Korean mugworts, Sajabal, Sajuari, and Injin, in relation to genus Artemisia. Direct sequencing of PCR products showed 642-3 bp of fully assembled ITS/5.8S region including 253-4 bp of ITS1, 167 bp of 5.8S rDNA, and 222 bp of ITS2. For a proper ITS analysis, the database of ambiguious sequences was modified either by making consensus sequence or by excluding the incomplete data. Although 5.8S rDNA was not informative site for taxonomic application within Artemisia due to high conservation, multiple alignment of ITS1 and ITS2 sequences indicated traditionally-named Sajuari mugwort to be A. verlotiorum.
Qualitative PCR Method for Detection of Genetically Modified Maize Lines NK603 and TC1507
Lee, Seong-Hun ; Park, Yong-Hwan ; Kim, Jin-Kug ; Park, Keun-Woo ; Kim, Young-Mi ;
Journal of Applied Biological Chemistry, volume 47, issue 4, 2004, Pages 185~188
Qualitative analytical method was developed using polymerase chain reaction (PCR) for detection of new lines of genetically modified (GM) maize, NK603 and TC1507. Two specific primer pairs were designed for qualitative PCR. To confirm specificity of designed primers, PCR was performed on genomic DNA extracted from new and other GM maizes, and cereal crops. Single PCR product was obtained from each designed primer pairs. For sensitivity determination of designed primers, test samples including NK603 and TC1507 GM lines were prepared at
levels and analyzed by PCR. Limit of detection (LOD) was 0.01% for both NK603 and TC1507 GM maizes. The results showed that the developed PCR method was useful for specific detection of NK603 and TC1507 lines of GM maize.
Subcellular Distribution of P and Ca in Arbuscular Mycorrhizal Plant: an X-Ray Microanalytical Study
Lee, Yun-Jeong ;
Journal of Applied Biological Chemistry, volume 47, issue 4, 2004, Pages 189~193
Energy dispersive X-ray microanalysis was used to determine elemental distribution of P and Ca in mycorrhizal and non-mycorrhizal cucumber roots. X-Ray microanalytical investigations revealed P contents was mainly detected in the arbuscular cytoplasm, and accumulated in poly phosphate granules, while the content in the arbuscular interface was significantly lower compared to those of the other cell compartments. High Ca content was detected in interfacial apoplast and cell wall compartments, while cytoplasmic contents in cells were lower. Interestingly, Ca was mostly accumulated in poly-P granules. X-Ray microanalytical investigations showed poly-P granules in AM fungal vacuole and interfacial apoplast between fungal and root cells could be regulating points for transfer of cations between symbionts, that is, from AM fungus to plant roots.
Evaluation of DNA Recovery from Soil and Sediment Samples
Kim, Soo-Jin ; Koo, Bon-Sung ; Park, In-Cheol ; Yoon, Sang-Hong ; Yun, Sang-Soon ; Sa, Tong-Min ; Yeo, Yun-Soo ;
Journal of Applied Biological Chemistry, volume 47, issue 4, 2004, Pages 194~198
Choice of protocol must be compromise between recovery of DNA most representative of microbial community and quality of DNA for construction of good metagenomic libraries. Four soil and sediment samples with various contents of organic matter (0.6 to 43%) were used to evaluate extraction and purification methods of DNA. Three methods of DNA extraction were compared: Method I, SDS method; Method II, freezing-thawing-SDS method; Method III, bead-beating-SDS method. Method III gave better DNA yield than I and II. Optimal bead-beating speed of method I to obtain maximum amount of high-molecular-weight DNA was 1,400 rpm although its DNA yield was slightly lower than with those obtained using other bead-beating speed. Several purification procedures were combined to evaluate DNA purity for enzyme digestion and PCR amplification. Gel-plus-column method gave very pure DNA, while gel-plus-centricon method gave highest recovery. Results indicate extraction and purification methods can be chosen depending on goal of the study.
Anti-fungal Activity of Bioconverted Oil Extract of Linoleic Acid and Fractionated Dilutions against Phytopathogens Rhizoctonia solani and Botrytis cinerea
Bajpai, Vivek ; Shin, Seung-Yong ; Kim, Min-Jeong ; Kim, Hak-Ryul ; Kang, Sun-Chul ;
Journal of Applied Biological Chemistry, volume 47, issue 4, 2004, Pages 199~204
Oil extract, bioconverted from linoleic acid by a bacterial strain Pseudomonas aeruginosa PR3, and its stepwise chromatographically fractionated dilutions were chemically analyzed by gas chromatography (GC) and thin layer chromatography (TLC), and evaluated for their anti-fungal activities against Botrytis cinerea and Rhizoctonia solani. The extract sample (
) showed high anti-fungal activity against R. solani (55%) and B. cinerea (53%) as compared to fractionated dilutions. Especially the fractionated dilutions of F2 (60 : 40) showed higher anti-fungal activities against B. cinerea (53%) as compared to R. solani (49%). Rest of fractionted dilutions showed relatively low anti-fungal activities against both the strains R. solani and B. cinerea (F1: 39%-43%, F3: 39%-47%, F4: 17%-49%, F5: 42%-50%) respectively. Oil extract sample showed anti-fungal activity with minimum inhibitory concentration (MIC), ranging from 500 to
. The growth inhibition percentages of oil extract of bioconverted linoleic acid and fractionated dilutions were measured as morphological abnormalities for both R. solani and B. cinerea involving lysis, distortion and swelling in hyphae, and also the screening was carried out using varied oil extract concentrations for the determination of antifungal effects on the spore germination of B. cinerea.
Antibacterial Activity of Various Hydroxy Fatty Acids Bioconveted by Pseudomonas aeruginosa PR3
Shin, Seung-Yong ; Kim, Hak-Ryul ; Kang, Sun-Chul ;
Journal of Applied Biological Chemistry, volume 47, issue 4, 2004, Pages 205~208
The antibacterial activity of hydroxylated fatty acids produced by Psuedomonas aeruginosa PR3 using ricinoleic acid, eicosadienoic acid and conjugated linoleic acid as substrates was investigated. The hydroxylated fatty acids showed different antibacterial activity against a range of food-borne pathogenic bacteria. The bioconverted ricinoleic acid exhibited the strong antibacterial activity against four Gram-positive bacteria, Bacillus subtilis ATCC 6633, Listeria monocytogenes ATCC 19166, Staphylococcus aureus ATCC 6538 and S. aureus KCTC 1916 and one Gram-negative bacteria, P. aeruginosa KCTC 2004. The bioconverted conjugated linoleic acid was slightly susceptible against 2 Gram-positive bacteria, B. subtilis ATCC 6633 and S. aureus KCTC 1916 and the bioconverted eicosadienoic acid against 4 Gram-positive bacteria, B. subtilis ATCC 6633, L. monocytogenes ATCC 19166, S. aureus ATCC 6538 and S. aureus KCTC 1916.