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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Applied Biological Chemistry
Journal Basic Information
Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 48, Issue 4 - Dec 2005
Volume 48, Issue 3 - Sep 2005
Volume 48, Issue 2 - Jun 2005
Volume 48, Issue 1 - Mar 2005
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Expression of Antioxidant Isoenzyme Genes in Rice under Salt Stress and Effects of Jasmonic Acid and
Kim, Jin-Hong ; Chung, Byung-Yeoup ; Baek, Myung-Hwa ; Wi, Seung-Gon ; Yang, Dae-Hwa ; Lee, Myung-Chul ; Kim, Jae-Sung ;
Journal of Applied Biological Chemistry, volume 48, issue 1, 2005, Pages 1~6
Analysis of chlorophyll (Chl) fluorescence implicated treatment of 40 mM NaCl decreased maximal photochemical efficiency of photosystem II (PSII) (Fv/Fm), actual quantum yield of PSII (
), and photochemical quenching (qP) in rice, but increased non-photochemical quenching (NPQ). Decreases in Fv/Fm,
, and qP were significantly alleviated by
jasmonic acid (JA), while NPQ increase was enhanced. Transcription levels of antioxidant isoenzyme genes were differentially modulated by NaCl treatment. Expression of cCuZn-SOD2 gene increased, while those of cAPXb, CATb, and CATc genes decreased. JA prevented salt-induced decrease of pCuZn-SOD gene expression, but caused greater decrease in mRNA levels of cAPXa and Chl_tAPX genes. Investigation of vacuolar
exchanger (NHX2) and 1-pyrroline-5-carboxylate synthetase (P5CS) gene expressions revealed transcription level of NHX2 gene was increased by JA, regardless of NaCl presence, while that of P5CS gene slightly increased only in co-presence of JA and NaCl. Unlike JA,
-radiation rarely affected expressions of antioxidant isoenzyme, NHX2, and P5CS genes, except for increase in mRNA level of Chl_tAPX and decrease in that of pCuZn-SOD. These results demonstrate enhanced salt-tolerance in JA-treated rice seedlings may be partly due to high transcription levels of pCuZn-SOD, NHX2, and P5CS genes under salt stress.
Purification and Characterization of
-L-Arabinosidase from Trichoderma sp. SY
Jung, Bo-Ra ; Kim, Bong-Gyu ; Lee, Yoon-Jung ; Ahn, Joong-Hoon ;
Journal of Applied Biological Chemistry, volume 48, issue 1, 2005, Pages 7~10
Trichoderma sp. SY most effectively produces an extracellular
-L-arabinofuranosidase (AF) using arabinose as a carbon source. AF grown on cellulose as a carbon source was purified 28-fold with 4.4% yield by DEAE exchange and HQ/20 cation exchange chromatographies The purified enzyme was found to be homogeneous on SDS-PAGE with molecular weight of 89 kDa. It exhibited a high level of activity with p-nitrophenyl
, respectively and did not require any metal ion for activity. It also released p-nitrophenol from p-nitrophenol conjugated
-D-galactopyranoside not from
Nitrate Uptakes by Microorganisms Isolated from the Soils of Greenhouse
Cho, Kwang-Hyun ; Lee, Gyeong-Ja ; Ahn, Hae-Jin ; Kim, Young-Kee ;
Journal of Applied Biological Chemistry, volume 48, issue 1, 2005, Pages 11~15
Salinity of soils in greenhouse has been increased by massive application of fertilizers. Nitrogen fertilizer was most popular, and thus nitrate became the majority of soil salinity. Accumulation of nitrate led to deleterious effects on the growth and development of crops and vegetables. Microbial strains able to utilize nitrate and thus remove excess nitrate from farm land soils were isolated from 15 different soils of greenhouses and plastic film houses. Four strains able to grow in medium containing 50 mM
were isolated, among which only E0461 showed high capacity of nitrate uptake. Nitrate uptake by E0461 was dependent on culture medium and was increased by addition of tryptone and peptone. Although E0461 was able to grow without tryptone and peptone, growth was slow, and no nitrate uptake was observed. Nitrate appeared to facilitate E0461 growth in the presence of tryptone and peptone. Through kinetic analysis, nitrate uptake was measured at various concentrations of nitrate, and half-life was calculated. Nitrate concentration decreased with increasing incubation period, and plot between half-lives and initial concentrations of nitrate fitted to single exponential function. These results suggest one major factor plays an important role in microbial nitrate uptake.
Development of an ELISA for the Detection of Fenazaquin Residues in Fruits
Lee, Jae-Koo ; Kim, Yun-Jung ; Lee, Eun-Young ; Kim, Dae-Kyu ; Kyung, Kee-Sung ;
Journal of Applied Biological Chemistry, volume 48, issue 1, 2005, Pages 16~25
To develop an enzyme-linked immunosorbent assay (ELISA) for the detection of the residues of the acaricide fenazaquin, five haptens were synthesized and assessed. A competitive indirect format was used with polyclonal antibodies. Under an optimized condition using the selected rabbit C antiserum, an
, the detection range of
, and the lowest detection limit of
were obtained. Some structurally related compounds of practical use showed low crossreactivities to the antibody. Highest cross-reactivity observed with hapten IV indicates that the antiserum C recognizes very well quinazoline ring, 4-tert-butylphenyl, and an adequate length of spacer arm. The length of spacer arm affected recognition of quinazoline ring and 4-tert-butylphenyl moieties. When applied to apple and pear, recoveries were within acceptable ranges of
(n = 4) and
(n = 4), respectively.
Larvicidal Activity of Chamaecyparis obtusa and Thuja orientalis Leaf Oils against Two Mosquito Species
Jeon, Ju-Hyun ; Lee, Sang-Hyun ; Kim, Moo-Key ; Lee, Hoi-Seon ;
Journal of Applied Biological Chemistry, volume 48, issue 1, 2005, Pages 26~28
Evaluation of larvicidal activities of Chamaecyparis obtusa and Thuja orientalis oils against 4th-instar larvae of Aedes aegypti and Culex pipiens pallens revealed larvicidal activities of leaf oils extracted from C. obtusa and T. orientalis were significantly higher than those of stem, fruit, and seed oils. Strong mortality was observed in age class II of C. obtusa and T. orientalis against Ae. aegypti and Cx. pipiens pallens larvae. These results show both leaf part and age class II of C. obtusa and T. orientalis have strong larvicidal activity against Ae. aegypti and Cx. pipiens pallens. Leaf oils of C. obtusa and T. orientalis leaves show promise as natural larvicides against Ae. aegypti and Cx. pipiens pallens.
Antimicrobial Activity of the Extracts of Forsythia suspensa and Dendranthema indicum
Li, Xing-Quan ; Zhang, Xin-Feng ; Lee, Kyu-Seung ;
Journal of Applied Biological Chemistry, volume 48, issue 1, 2005, Pages 29~31
Antimicrobial activities of extracts of Forsythia suspensa fruits and Dendranthema indicum buds and flowers against bacteria; Escherichia coli, Staphyloccus aureus, and Bacillus subtilis, and fungi; Aspergillus flavusn, Rhizopus stolonifer, Penicillium citrinum, Aspergillus niger, and Saccharomyces carlsbergensis, were investigated. The plants were extracted with 70% ethanol and the extracts were used for antimicrobial activity assay. All extracts exhibited significant inhibition activity against microorganisms at concentrations ranged from 1.66 to
. The inhibition activity by the extract of D. indicum buds was stronger than by the extract of F. suspensa fruits and D. indicum flowers. D. indicum buds showed antimicrobial activity against S. aureus which was comparable to other medicinal plants. F. suspensa fruits and D. indicum flowers was suggested to be valuable sources as antimicrobial ingredients in food industry.
5-(Hydroxymethyl)-2-furfuraldehyde, Anticonvulsant Furan from the Arils of Euphoria longana L.
Kim, Dong-Hyun ; Kim, Dae-Won ; Choi, Soo-Young ; Park, Chang-Ho ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 48, issue 1, 2005, Pages 32~34
Arils of Euporia longana L. was extracted with 80% aqueous MeOH and partitioned successively with EtOAc, n-BuOH and
. From the n-BuOH fraction, furan compound was isolated through silica gel column chromatography. The results of physico-chemical data including NMR, MS and IR revealed the compound to be 5-(hydroxymethyl)-2-furfuraldehyde. This compound stimulated GDH I activity by
at in vitro concentrations of 0.005, 0.008, 0.02 and 0.03 %, respectively.
Development of Biologically Active Compounds from Edible Plant Sources XIV. Cyclohexylethanoids from the Flower of Campsis grandiflora K. Schum.
Kim, Dong-Hyun ; Oh, Young-Jun ; Han, Kyung-Min ; Chung, In-Sik ; Kim, Dae-Keun ; Kim, Sung-Hoon ; Kwon, Byoung-Mog ; Park, Mi-Hyun ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 48, issue 1, 2005, Pages 35~37
Campsis grandiflora K. Schum. flower was extracted with 80% aqueous MeOH, and concentrated extract was successively partitioned with EtOAc, n-BuOH, and
. From n-BuOH fraction, two cyclohexylethanoids were isolated through repeated silica gel and Sephadex LH-20 column chromatographies. Based on physico-chemical data obtained from NMR, MS, and IR, chemical structures of compounds were determined as 1,4-dihydroxy-3,4-(epoxyethano)-5-cyclohexene (1) and cornoside (2). These compounds were isolated for the first time from C. grandiflora K. Schum flower.
Sterin C, a New Antioxidant from the Mycelial Culture of the Mushroom Stereum hirsutum
Yoo, Nam-Hee ; Yoo, Ick-Dong ; Kim, Jin-Woo ; Yun, Bong-Sik ; Ryoo, In-Ja ; Yoon, Eui-Soo ; Chinh, Nguyen Thi ; Kim, Jong-Pyung ;
Journal of Applied Biological Chemistry, volume 48, issue 1, 2005, Pages 38~41
Structurally new antioxidative metabolite was isolated from mycelial culture of mushroom Stereum hirsutum. Culture broth was subjected to Diaion HP-20 column chromatography, and 70% aqueous MeOH eluent was extracted with EtOAc. EtOAc extract was purified through silica gel and Sephadex LH-20 column chromatographies, and reversed phase
HPLC. Compound was revealed to be new dihydroxylated derivative of sterin B with molecular formula of
(MW 240) by MS and various NMR spectral data analyses, and designated as sterin C. Sterin C showed superoxide radical-scavenging activity with
value of 0.31 mM.