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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
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Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 48, Issue 4 - Dec 2005
Volume 48, Issue 3 - Sep 2005
Volume 48, Issue 2 - Jun 2005
Volume 48, Issue 1 - Mar 2005
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Soy Isoflavones and Soyasaponins: Characteristics and Physiological Functions
Lee, Yoon-Bok ; Lee, Hyong-Joo ; Kim, Chung-Ho ; Lee, Soo-Bok ; Sohn, Heon-Soo ;
Journal of Applied Biological Chemistry, volume 48, issue 2, 2005, Pages 49~57
Soy is an important food in Asia and many studies have suggested that the low incidences of chronic diseases in Asian countries are associated with diets that are rich in soy. Soy contains many kinds of phytochemicals, and soy isoflavones and soyasaponins have received considerable attention. Twelve isoflavone components have been isolated from soy: three aglycones (daidzein, genistein, and glycitein), and their respective nine glucosidic conjugates. Soy isoflavones are similar in structure to estrogen and exhibit both estrogenic and antiestrogenic activities. Soy isoflavones exhibit anticancer activity, can reduce the risk of cardiovascular disease, and are beneficial to brain and bone health. Soyasaponins are divided into three groups (A, B, and E saponins), and they exhibit hypocholesterolemic, anticancer, hepatoprotective, antioxidative, and anti-human-immunodeficiency-virus effects. Despite the abundant literature suggesting that soy isoflavones and soyasaponins have potential applications in preventive medicine, further research is needed to standardize dosages and ensure their efficacy.
Pulsed Field Gel Electrophoresis for Subtyping of Listeria monocytogenes
Jang, Sung-Sik ; Fleet, Graham H. ; Cox, Julian M. ;
Journal of Applied Biological Chemistry, volume 48, issue 2, 2005, Pages 58~64
Listeria monocytogenes is a high-risk foodborne pathogen responsible for foodborne listeriosis outbreaks, and is particularly dangerous to immuno-compromised people with mortality rate of about 30%. This review summarizes subtyping of L. monocytogenes using Pulsed Field Gel Electrophoresis, widely used to trace origin of foodborne outbreaks and to determine relationship between isolates.
Pesticidal Constituents Derived from Piperaceae Fruits
Lee, Hoi-Seon ;
Journal of Applied Biological Chemistry, volume 48, issue 2, 2005, Pages 65~74
Fungicidal, insecticidal, and mosquito larvicidal activities of piperidine alkaloids, pipernonaline and piperoctadecalidine, and isobutylamide alkaloids, pellitorine, guineensine, pipercide, and retrofractaminde A, derived from Piperaceae fruits were studied. Pipernonaline and piperoctadecalidine showed potent fungicidal activities against Puccinia recondita with 91 and 80% control values at 500 ppm. Against Phytophthora infestans, pipernonaline showed strong fungicidal activity with 91 and 80% control values at 1,000 and 500 ppm.
values of pipernonaline and piperoctadecalidine against Plutella xylostella were 125 and 95.5 ppm, respectively, and that of piperoctadecalidine against Tetranychus urticae was 246 ppm. Against larvae of Aedes aegypti and Culex pipiens pallens,
values of pipernonaline were 0.35 and 0.21 ppm, respectively. Highest larvicidal activities of pipercide and retrofractamide A were found against A. aegypti, A. togoi, and C. pipiens pallens.
values of pipercide and retrofractamide A were 0.10 and 0.039 ppm against A. aegypti, 0.26 and 0.01 ppm against A. togoi, and 0.004 and 0.028 ppm against C. pipiens pallens, respectively. Based upon these results and earlier findings, bioactive components derived from Piperaceae fruits may be valuable for development of useful lead product of possibly safer fungicidal, insecticidal, and mosquito larvicidal agents.
Partial Purification and Characterization of Halotolerant Alkaline Protease from Halomonas marisflava KCCM 10457 Isolated from Salt-fermented Food
In, Man-Jin ; Oh, Nam-Soon ; Kim, Dong-Chung ;
Journal of Applied Biological Chemistry, volume 48, issue 2, 2005, Pages 75~78
Halotolerant protease produced by Halomonas marisflava KCCM 10457 was partially purified through ammonium sulfate precipitation and Sephacryl S-200HR gel permeation chromatography. Optimal pH and temperature of protease were 11.0 and
, respectively. Enzyme activity was inhibited by
, and selectively inhibited by p-chloromercuribenzoic acid (PCMB), suggesting this enzyme is cysteine protease. The enzyme is halotolerant, because it retained 77% of original activity in presence of 3.33 M NaCl. The protease showed broad substrate specificity to various natural proteins; BSA, casein, egg albumin, gelatin, and hemoglobin.
Plant Growth Promotion and Suppression of Root Disease Complex due to Meloidogyne incognita and Fusarium oxysporum by Fluorescent Pseudomonads in Tomato
Kumar, Tarun ; Bajpai, Vivek K. ; Maheshwari, Dinesh Kumar ; Kang, Sun-Chul ;
Journal of Applied Biological Chemistry, volume 48, issue 2, 2005, Pages 79~83
While screening for nematicidal activity of bacterial origins, various pseudomonads strains were inhabited in tomato rhizosphere. One isolate designated as
was selected for studies on nematicidal properties and plant growth-promoting (PGP) activity and was identified as Pseudomonas aeruginosa based on morphological features, biochemical and physiological tests, and carbohydrate utilization. To investigate nematicidal activity, Meloidogyne incognita juvenile mortality was determined using
culture filtrate. Inhibition of strain
against Fusarium oxysporum was observed using dual culture technique. Strain
showed good siderophore activity, HCN and IAA production abilities, and growth and development enhancement of tomato.
Studies on the Inhibitory Effect of Eugenia aromaticum Extract on Pancreatic Lipase
Kim, Seung-Kyum ; Kim, Yong-Mu ; Hong, Mi-Jeong ; Rhee, Hae-Ik ;
Journal of Applied Biological Chemistry, volume 48, issue 2, 2005, Pages 84~88
To develop functional food and anti-obesity drug through inhibition of dietary lipid absorption, inhibitory effects of herb extracts on pancreatic lipase were investigated. Due to high yield and simplicity of isolation, lipase inhibitor (ELI) was isolated from ethyl acetate extract of Eugenia aromaticum, which showed highest inhibitory activity, and characterized for development of novel functional material. Stability of ELI at high temperature and low pH was investigated. Results showed ELI is relatively stable under thermal and acidic conditions, reversible, and noncompetitive inhibitor of pancreatic lipase.
Germination-Inhibitory Effect of Pulsatilla koreana N. Leaves; Protoanemonin as Active Principle
Bang, Seong-Cheol ; Kim, Dong-Hwa ; Ahn, Byung-Zun ;
Journal of Applied Biological Chemistry, volume 48, issue 2, 2005, Pages 89~92
The germination of Lactuca sativa seeds was significantly inhibited by the water extract of the fresh leaves of Pulsatilla koreana N. including abundant ranunculin. Germination inhibitory activity increased in a dose-dependantly. Protoanemonin, produced from ranunculin by enzymatic action during maceration process of leaves, was proved to be the active principle with inhibitory activity was above 90% at concentration of 1 mg/ml.
Phytochemical Constituents from the Stem Bark of Phellodendron amurense Rupr.
Lee, Jin-Hwan ; Lee, Byong-Won ; Moon, Yea-Hwang ; Yang, Min-Suk ; Jang, Ki-Chang ; Park, Ki-Hun ;
Journal of Applied Biological Chemistry, volume 48, issue 2, 2005, Pages 93~96
Three limonoids and two phenolics were isolated from stem bark of Pellodendron amurense Rupr. (Rutaceae) and were defined clearly as calodendrolide (1), obacunone (2), limonin (3), methyl 4-hydroxybenzoate (4), and syringin (5) based on NMR spectroscopy results obtained with the aid of X-ray crystallographic analysis. This is the first report on the isolation of calodendrolide (1) from this plant.
Facial Synthesis of Versatile Chiral Norbornenes as Leukotriene D4 Antagonists from D-glucose
Lim, Yoong-Ho ; Koh, Dong-Soo ;
Journal of Applied Biological Chemistry, volume 48, issue 2, 2005, Pages 97~100
Chiral dienophile 5 was synthesized from D-glucose by consecutive diisopropylidenation, partial deprotection, diol cleavage, and Wittig reactions. Under thermal conditions, asymmetric Diels-Alder reaction between chiral dienophile and cyclopentadiene gave four possible chiral norbornenes stereoisomers whose absolute configurations were determined through CADD and NMR.
Functional Identification of Ginkgo biloba 1-Deoxy-D-xylulose 5-Phosphate Synthase (DXS) Gene by Using Escherichia coli Disruptants Defective in DXS Gene
Kim, Sang-Min ; Kuzuyama, Tomohisa ; Chang, Yung-Jin ; Kim, Soo-Un ;
Journal of Applied Biological Chemistry, volume 48, issue 2, 2005, Pages 101~104
DXS catalyzes the first step of MEP pathway. Escherichia coli disruptants defective in dxs were constructed by insertional mutation and characterized. Selected disruptant, DXM3, was auxotrophic for DX or ME. Putative class 1 DXS ORF from Ginkgo biloba was shown to rescue DXM3 grown without DX or ME supplementation. The putative ORF was thus confirmed as DXS1. The disruptant was demonstrated to be useful for DSX screening.
Application of Multicomponent Reactions in Synthesis of Diimines as Salen Homologues
Koh, Dong-Soo ;
Journal of Applied Biological Chemistry, volume 48, issue 2, 2005, Pages 105~107