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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
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Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 48, Issue 4 - Dec 2005
Volume 48, Issue 3 - Sep 2005
Volume 48, Issue 2 - Jun 2005
Volume 48, Issue 1 - Mar 2005
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O-Methyltransferases from Arabidopsis thaliana
Kim, Bong-Gyu ; Kim, Dae-Hwan ; Hur, Hor-Gil ; Lim, Jun ; Lim, Yoong-Ho ; Ahn, Joong-Hoon ;
Journal of Applied Biological Chemistry, volume 48, issue 3, 2005, Pages 113~119
O-methylation mediated by O-methyltransferases (OMTs) is a common modification in natural product biosynthesis and contributes to diversity of secondary metabolites. OMTs use phenylpropanoids, flavonoids, other phenolics and alkaloids as substrates, and share common domains for S-adenosyl-L-methionine (AdoMet) and substrate binding. We searched Arabiposis genome and found 17 OMTs genes (AtOMTs). AdoMet- and substrate-binding sites were predicted. AdoMet binding domain of AtOMTs is highly conserved, while substrate-binding domain is diverse, indicating use of different substrates. In addition, expressions of six AtOMT genes in response to UV and in different tissues were investigated using real-time quantitative reverse transcriptase-polymerase chain reaction. All the AtOMTs investigated were expressed under normal growth condition and most, except AtOMT10, were induced after UV illumination. AtOMT1 and AtOMT8 were expressed in all the tissues, whereas AtOMT10 showed flower-specific expression. Analysis of these AtOMT gene expressions could provide some clues on AtOMT involvement in the cellular processes.
Independent Regulation of Endothelial Nitric Oxide Synthase by Src and Protein Kinase A in Mouse Aorta Endothelial Cells
Boo, Yong-Chool ;
Journal of Applied Biological Chemistry, volume 48, issue 3, 2005, Pages 120~126
Endothelial nitric oxide synthase (eNOS) plays a critical role in vascular biology and pathophysiology. Its activity is regulated by multiple mechanisms such as calcium/calmodulin, protein-protein interactions, sub-cellular locations and phosphorylation at various sites. Phosphorylation of eNOS-Ser1177 (based on mouse sequence) has been identified as an important mechanism of eNOS activation. However, signaling pathway leading to it phosphorylation remains controversial. The regulation of eNOS-Ser1177 phosphorylation by Src and protein kinase A (PKA) was investigated in the present study using cultured mouse aorta endothelial cells. Expression of a constitutively active Src mutant in the cells enhanced phosphorylation of eNOS and protein kinase B (Akt). The Src-stimulated phosphorylation was not attenuated by the expression of a dominant negative PKA regulatory subunit. Neither activation nor inhibition of PKA activity had any significant effect on tyrosine phosphorylation of activation or inactivation site in Src. Based on the results of this study, it is suggested that Src/Akt pathway and PKA signaling may regulate eNOS phosphorylation independently. The existence of multiple mechanisms for eNOS phosphorylation may guarantee endothelial nitric oxide production in various cellular contexts which is essential for maintenance of vascular health.
Classification and Expression Profiling of Putative R2R3 MYB Genes in Rice
Kim, Bong-Gyu ; Ko, Jae-Hyung ; Min, Shin-Young ; Ahn, Joong-Hoon ;
Journal of Applied Biological Chemistry, volume 48, issue 3, 2005, Pages 127~132
MYB genes, comprising group of related genes found in animal, plant, and fungal genomes, encode common DNA-binding domains composed of one to four repeat motifs. MYB genes containing two repeats (R2R3) constitute largest MYB gene family in plants. R2R3 MYB genes play important roles in regulation of secondary metabolism, control of cell shape, disease resistance, and hormone response. Eight-four R2R3 MYB genes were retrieved from rice genome for functional characterization of MYB genes. Analysis of MYB domains revealed each MYB domain contains three
-helices with regularly spaced tryptophan residues. R2R3 MYB genes were divided into four subfamilies based on phylogenic analysis result. Real-time PCR analysis of 34 MYB genes revealed 12 MYB genes were highly expressed in seeds than in leaves, whereas 4 genes were highly expressed in leaves.
Antimutagenic Effect of Bacillus natto Isolated from Natto
Yun, Soon-Il ;
Journal of Applied Biological Chemistry, volume 48, issue 3, 2005, Pages 133~137
In vitro antimutagenicity of Bacillus natto islolated from Natto, Japanese traditional fermented food, was investigated using umu-test. Mutagenicity of S9-activated metabolites of Trp-P-2 and IQ for Salmonella typhimurium TA 1535/pSK1002 was remarkably inhibited by addition of bacterial cells and their cytoplasmic fraction. Desmutagenicity by cytoplasmic fraction increased with increasing concentration of the fraction. Bioantimutagenic effect of cytoplasm on Salmonella typhimurium SD-100 did not show bioantimutagenic activity against mutated bacterial cells induced by Trp-P-2. Cytoplasmic fraction exhibited 17% bioantimutagenicity due to desmutation caused by IQ.
Antibacterial, Free Radical Scavenging, and Proliferative Effects of Korean Fermented Soybean Paste (Doenjang) Extracts
Yun, Soon-Il ;
Journal of Applied Biological Chemistry, volume 48, issue 3, 2005, Pages 138~143
Antibacterial, free radical scavenging, proliferative, and cytotoxicity effects of Doenjang extracts were examined. All samples except water extract showed strong antibacterial activity against oral bacteria, Streptococcus. pyogenes, S. mutants, S. sanguinis, S. sorbrinus, S. criceti, S. antinosus, S. gordonii, and Porphyromonas. gingivalis (MIC and MBC values: 0.08-1.25 and 0.16-2.50 mg/ml, respectively). DPPH method showed ethanol and ethyl acetate extracts are effective inhibitors of oral bacteria. Based on MTT assay, 24 h exposure to 0.31 mg/ml of all extracts, excepted water extract, resulted in strong cytotoxicity on KB cells. All extracts strongly inhibited human gingival fibroblast viability.
Comparison of Tyrosinase Inhibitory Effect of the Natural Antioxidants from Cedrela sinensis
Hwang, Seon-Woo ; Lee, Jun ; Kwon, Hyun-Sook ; Lee, Kyung-Dong ; Nam, Sang-Hae ; Park, Ki-Hun ; Yang, Min-Suk ;
Journal of Applied Biological Chemistry, volume 48, issue 3, 2005, Pages 144~147
DPPH and ABTS radical scavenging, and tyrosinase inhibitory activities of bioactive compounds 1-4 isolated from Cedrela sinensis leaf were assessed. Structures of isolated compounds were established by UV, one- and two-dimensional NMRs, and mass spectroscopic methods.
Solution Structure of Human Atrial Natriuretic Peptides (7-28)
Lee, Yu-Kyung ; Lim, Yoong-Ho ;
Journal of Applied Biological Chemistry, volume 48, issue 3, 2005, Pages 148~150
Antifungal Activity of Bacillus sp. against Pepper Anthracnose
Yi, Yong-Sub ; Kim, Hye-Sook ; Choi, Gyung-Ja ; Cho, Kwang-Yun ; Lim, Yoong-Ho ;
Journal of Applied Biological Chemistry, volume 48, issue 3, 2005, Pages 151~152