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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
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Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 48, Issue 4 - Dec 2005
Volume 48, Issue 3 - Sep 2005
Volume 48, Issue 2 - Jun 2005
Volume 48, Issue 1 - Mar 2005
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Nematicidal Activity of Some Fluorescent Pseudomonads on Cyst Forming Nematode, Heterodera cajani and Growth of Sesamum indicum var. RT1
Kumar, Tarun ; Kang, Sun-Chul ; Maheshwari, Dinesh Kumar ;
Journal of Applied Biological Chemistry, volume 48, issue 4, 2005, Pages 161~166
Among 24 isolates of fluorescent Pseudomonads, 5 isolates named as LPT1, LPT2, LPT3, LPT4 and LPT5 were screened in vitro for their nematicidal activity against cyst forming nematode, Heterodera cajani causing patchiness, poor and stunting growth besides discoloration in Sesamum indicum. Second stage juveniles of H. cajani hatched from egg masses were collected from roots of host plant and subjected to fresh and heat-treated culture filtrate of isolates for 24 h. Mortality of H. cajani was recorded on the basis of parameters used for test organism bioassay. Among these isolates, Pseudomonas aeruginosa LPT5 caused maximum mortality towards second stage juvenile of H. cajani in vitro. Five isolates were used as seed coating for the management of cyst forming nematode H. cajani on sesame in green house condition. The strains LPT5 was better than the other strains in reducing the population of H. cajani both in vitro and in vivo. The reduction in cyst and juveniles population was found to be 49 and 60%, respectively when seeds were coated with strain LPT5. Among other strains, LPT4 was also found to inhibit the cyst and juveniles population 12 and 36% respectively. Increases in early vegetative plant growth parameters recorded in both in vitro and in vivo further revealed the significance of indigenous bacteria in comparison to introduced strain.
Antibacterial Activity of Bioconverted Linoleic Acid Produced by Pseudomonas aeruginosa PR3
Shin, Seung-Young ; Bajpai, Vivek K. ; Hou, Ching T. ; Choi, Ung-Kyu ; Kim, Hak-Ryul ; Kang, Sun-Chul ;
Journal of Applied Biological Chemistry, volume 48, issue 4, 2005, Pages 167~169
Crude extract of bioconverted linoleic acid using Pseudomonas aeruginosa PR3 was evaluated for its antibacterial activity against food-borne pathogenic bacteria. Crude extract showed antibacterial activity against four Gram-positive bacteria, Staphylococcus aureus (ATCC 6538), S. aureus (KCTC 1916), Listeria monocytogenes (ATCC 19166), and Bacillus subtilis (ATCC 6633), and one Gramnegative bacterium, Pseudomonas aeruginosa (KCTC 2004), with minimum inhibitory concentrations ranging from 750 to
. S. aureus and B. subtilis were selected for growth inhibition assays with bioconverted linoleic acid. Major antibacterial effects occurred at lag phase.
Prevention of Murine Acquired Immunodeficiency Syndrome (MAIDS) Development by Oriental Herb Extracts
Yang, Yun-Hee ; Yang, Joo-Sung ;
Journal of Applied Biological Chemistry, volume 48, issue 4, 2005, Pages 170~177
Oriental medicinal herb extracts (OHE) showing anticancer activities were investigated for effectiveness as antiviral drugs. Infection of MuLV to cell line resulted in formation of giant syncytia. Number of giant syncytia in culture treated with OHE decreased by 40% compared to that of non-OHE-treated cell culture. To determine OHE effects on progeny release, RT-PCR was performed. In vivo animal studies demonstrated effectiveness of OHE as antiviral drug when administered orally. After OHE administration, viral cytopathic effects decreased. Infected mice showed splenomegaly and over-proliferation of lymphocytes with decreased CD4+ cell counts. These symptoms decreased in OHE-treated mice, indicating OHE maybe useful therapeutics against MuLV/MAIDS as Human Immunodeficiency Virus (HIV)/AIDS animal model. Results show XC plaque assay and in vivo MAIDS model using MuLV are suitable tools for screening anti-retroviral drug candidates.
Stimulation of eNOS-Ser617 Phosphorylation by Fluid Shear Stress in Endothelial Cells
Boo, Yong-Chool ;
Journal of Applied Biological Chemistry, volume 48, issue 4, 2005, Pages 178~182
Nitric oxide (NO) produced from endothelial cells plays a critical role in vascular physiology. The regulation of endothelial NO synthase (eNOS) involves various mechanisms including multiple Ser/Thr phosphorylations. Recently, eNOS-Ser617 was newly recognized to be phosphorylated in response to humoral factors including vascular endothelial growth factor. However, it remains unknown whether and how eNOS-Ser617 phosphorylation is stimulated by shear stress, the primary stimulus of endothelial NO production. This issue was explored in the present study using cultured bovine aortic endothelial cells (BAECs). Over-expression of a constitutively active protein kinase B(Akt) mutant in BAECs increased Ser617 phosphorylation while constitutively active protein kinase A mutant had no effect. When BAECs were subjected to an arterial level of laminar shear stress, eNOS-Ser617 phosphorylation was clearly increased in a time-dependent manner. Shear stress also stimulated Akt phosphorylation at Thr308, one of the key regulatory sites. The time courses of eNOS-Ser617 and Akt-Thr308 phosphorylations appeared to be very similar. These results suggested that eNOS-Ser617 phosphorylation, mediated by Akt, is a physiological response to the mechanical shear stress, involved in the regulation of NO production in endothelial cells.
Complementation of E. coli cysQ Mutant with Arabidopsis AHL Gene Encoding a 3'(2'),5'-Bisphosphate Nucleotidase
Cheong, Jong-Joo ; Kwon, Hawk-Bin ;
Journal of Applied Biological Chemistry, volume 48, issue 4, 2005, Pages 183~186
Arabidopsis AHL gene encodes a 3'(2')-phosphoadenosine 5'-phosphate (PAP)-specific phosphatase that plays a role in the sulfate activation pathway. We complemented E. coli cysQ mutant defective in cysteine biosynthesis with the AHL gene. AHL cDNA was cloned into the prokaryotic expression vector pKK388-1 and transformed into the bacterial mutant. Since cysQ mutant is a leaky cysteine auxotroph only under aerobic conditions, the bacteria were grown in liquid media with vigorous shaking to provide more aeration. In cysteine-free medium, cysQ mutant and the mutant harboring empty vector did not grow well, whereas cells harboring AHL cDNA exhibited significantly improved growth with doubling time of approximately 3 h. cysQ is known to encode a 3'(2'),5'-diphosphonucleoside 3'(2')-phosphohydrolase (DPNPase). However, our data suggest that cysQ protein has PAP-specific phosphatase activity in addition to DPNPase activity. Microbial complementation procedure described in this paper is useful for structure-activity studies of PAP-specific phosphatases identified from microbes and plants.
Impact of Herbicide Oxadiazon on Microbial Activity and Nitrogen Dynamics in Soil Environment
Rahman, Md. Mokhlesur ; Song, Kyung-Sik ; Rhee, In-Koo ; Kim, Jang-Eok ;
Journal of Applied Biological Chemistry, volume 48, issue 4, 2005, Pages 187~192
Influence of herbicide oxadiazon on soil microbial activity and nitrogen dynamics was evaluated. Soil samples were treated with oxadiazon at field and tenfold field rates and incubated. Organic amendment was added as an additional substrate for soil microorganisms. Tenfold field rate oxadiazon stimulated substrate-induced respiration (SIR) and dehydrogenase activity (DHA) in amended soil as compared to unamended soil and control treatment. Soil urease activity was not affected by oxadiazon treatment. In both amended and unamended soils, treatment of the herbicide at higher rate had not significant influence on
-N concentrations. Higher dose of oxadiazon was degraded in both soils, but dissipation rate in amended soil was higher than unamended soil, with half-lives (
) of 23.1 and 138.6 days, respectively. Recommended field rate did not affect microbial activity and nitrogen dynamics in soil ecosystem. Results showed influence of oxadiazon on cycling processes of nitrogen in soil was not significant however its effect on microbial activity was a tendency depending on addition of organic amendment to soil.
Antioxidant Effects of Isoflavones from the Stem Bark of Cudrania tricuspidata
Lee, Jin-Hwan ; Lee, Byong-Won ; Kim, Jin-Hyo ; Seo, Woo-Duck ; Jang, Ki-Chang ; Park, Ki-Hun ;
Journal of Applied Biological Chemistry, volume 48, issue 4, 2005, Pages 193~197
Isoflavones 4'-O-methylalpinumisoflavone (1) and 5, 7, 4'-trihydroxy-6,8-diprenylisoflavone (2) were isolated from stem bark of Cudrania tricuspidata (Carr.) Bureau. These compounds were the first reported from this plant. Antioxidant activities 1 and 2 were evaluated by measuring their ability to scavenge DPPH and ABTS radicals. Diprenylisoflavone 2 showed strong scavenging activity against ABTS (
), three-fold higher compared to genistein, which do not possess prenylated group which indicates strong scavenging activity against ABTS radical of isoflavone 2 was due to prenyl group.
Cytotoxic Isoquinoline Alkaloids from Chelidonium majus var. asiaticum
Lee, Jun ; Shon, Mi-Yae ; Jang, Dae-Sik ; Ha, Tae-Joung ; Hwang, Seon-Woo ; Nam, Sang-Hae ; Seo, Eun-Kyoung ; Park, Ki-Hun ; Yang, Min-Suk ;
Journal of Applied Biological Chemistry, volume 48, issue 4, 2005, Pages 198~201
Two known isoquinoline alkaloids, (+)-chelidonine (1) and (-)-stylopine (2), were isolated from
-soluble fraction of whole plants of Chelidonium majus L. var. asiaticum, and their structures were identified by spectroscopic methods and X-ray crystallographic analysis. Two isolates (1 and 2) were examined for their in vitro cytotoxic activities against five human cancer cell lines including DU-145 (prostate), MCF (breast), A549 (lung), HePG2 (liver), and HT-29 (colon) by sulphorhodamine B (SRB) assay.
Boiled-Water Extract from Hizikia fusiformis Showing Antioxidant Effects
Jang, Mi-Soon ; Kim, Ji-Young ; Lim, Chi-Won ; Kim, Yeon-Kye ; Park, Hee-Yeon ;
Journal of Applied Biological Chemistry, volume 48, issue 4, 2005, Pages 202~206
Boiled-water extract (BWE) of Hizikia fusiformis has antioxidant effects. To investigate antioxidant effects of BWE, 1,1-dephenyl-2-picrylhydrazyl (DPPH) and thiobarbituric acid (TBA) methods were used. BWE exhibited strong radical-scavenging activity (87.3% at final conc.
BWE/ml in an assay mixture) on DPPH and good inhibitory activity (64% at final conc.
BWE/ml in an assay mixture) on lipid peroxidation. Raw 264.7 cells treated with lipopolysaccharide (LPS) showed 26.7-fold increase in nitric oxide (NO) (
) compared with control group (
). When treated with BWE and LPS, NO production was inhibited by BWE dose-dependently.
Prolyl Endopeptidase Inhibitory Activity of Ursolic and Oleanolic Acids from Corni Fructus
Park, Yoon-Seok ; Jang, Hyun-Jung ; Paik, Young-Sook ;
Journal of Applied Biological Chemistry, volume 48, issue 4, 2005, Pages 207~212
Prolyl endopeptidase (PEP, EC 184.108.40.206), also referred to as prolyl oligopeptidase, has been suggested to participate in learning and memory processes by cleaving peptide bonds on carboxyl side of prolyl residue within neuropeptides of less than 30 amino acids, and is abundant in brains of amnestic patients. Therefore, compounds possessing PEP inhibitory activity can be good candidate of drug against memory loss. Upon examination for PEP inhibition from traditional medicinal plants having tonic, stimulating, and anti-amnestic effects, Corni Fructus (Cornus officinallis) showed significant PEP inhibition. Ursolic and oleanolic acids, components of Corni Fructus, inhibited PEP with
Characteristics of Hydrogel Prepared from Microbial Poly(
-glutamic acid) by Chemical Crosslinker
Park, Jong-Soo ; Choi, Seong-Hyun ; Choi, Woo-Young ; Yoon, Min-Ho ;
Journal of Applied Biological Chemistry, volume 48, issue 4, 2005, Pages 213~217
Microbial hydrogel was prepared with poly(
-glutamic acid) produced from Bacillus subtilis BS62 using crosslinking reagent, ethylene glycol diglycidyl ether (EGDE), and its physico-chemical characteristics were examined. Hydrogel which prepared from 10 grams of 10% PGA solution with
of EGDE at
for 17 h swelled 4,320 times its dry weight, and time to reach swelling equilibrium in deionized water at 4 to
range was about 20 h. Swollen hydrogel shrunk in ionic solutions, and rate of shrinkage was higher in calcium chloride solution than sodium chloride solution. Swelling rate of hydrogel increased 1.3-fold of initial swelling rate for 30 min at
Nucleotide Sequence and Characterization of ptsG Gene Encoding Glucose-specific Enzyme II of Phosphotransferase System from Brevibacterium flavum
Yoon, Ki-Hong ;
Journal of Applied Biological Chemistry, volume 48, issue 4, 2005, Pages 218~221
Nucleotide sequence of Brevibacterium flavum ptsG gene capable of complementing Escherichia coli ZSC113 mutations defective to glucose permease activity of phosphotransferase system was completely determined, and the gene product was compared with other glucose-specific enzyme II (
). A ptsG gene of B. flavum consisted of open reading frame of 2,025 nucleotides putatively encoding polypeptide of 675 amino acid residues and TAA stop codon. Deduced amino acid sequence of B. flavum (
) had high homology with (
) of Corynebacterium glutamicum, C. efficiens, and B. lactofermentum. Arrangement of structural domains, IIBCA, of B. flanum (
) protein was identical to that of EIIs belonging to glucose-phosphotransferase system.
An Antifungal Activity of Streptomyces sp. against Cryphonectria parasitica
Lee, Sang-Hyun ; Kim, Kyung-Hee ; Lee, Jae-Pil ; Yi, Yong-Sub ; Kim, Hye-Sook ; Lim, Yoong-Ho ;
Journal of Applied Biological Chemistry, volume 48, issue 4, 2005, Pages 222~225
Strains showing antifungal activity against Cryphonectria parasitica were isolated from coast soil of Taean , Korea. Of 152 strains isolated, 6 strains showed antifungal activity in vitro against C. parasitica. Ta24 strain showed highest activity with 1.6 cm clean inhibition zone. For strain identification, the morphological characteristic and 16S rDNA sequences were determined. Ta24 strain showed 99% homology with Streptomyces sampsonii and was identified as Streptomyces sp.
Hydrogen Peroxide Effect on Agrobacterium-Mediated Alfalfa Sprouts Transformation
Shin, Dong-Il ; Park, Hee-Sung ;
Journal of Applied Biological Chemistry, volume 48, issue 4, 2005, Pages 226~228
Transient Expression in Chinese Cabbage by Hydrogen Peroxide-aided Agroinfiltration
Shin, Dong-Il ; Park, Hee-Sung ;
Journal of Applied Biological Chemistry, volume 48, issue 4, 2005, Pages 229~230