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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
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Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 49, Issue 4 - Dec 2006
Volume 49, Issue 3 - Sep 2006
Volume 49, Issue 2 - Jun 2006
Volume 49, Issue 1 - Mar 2006
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Molecular Cloning and Expression of Dihydroflavonol 4-reductase Gene in Tuber Organs of Purple-fleshed Potatoes
Kang, Won-Jin ; Lee, Yong-Hwa ; Kim, Hyun-Soon ; Joung, Hyouk ; Jeon, Jae-Heung ;
Journal of Applied Biological Chemistry, volume 49, issue 3, 2006, Pages 75~81
A full-length cDNA encoding dihydroflavonol 4-reductase (st-dfr) of potato was isolated by rapid amplification of cDNA ends, and their expression was investigated from purple-fleshed potato (Solanum tuberosum L. cv. Jashim). The st-dfr exists as a member of a small gene family and its transcripts was abundant in the order of tuber flesh, stem, leaf, and root. The expressions of st-dfr gene were light inducible and cultivar dependant. Transgenic potato plants harboring antisense st-dfr (AS-DFR) sequences were analyzed. The accumulation of mRNA was nearly completely inhibited as a result of introducing an AS-DFR gene under the control of the 35S CaMV promoter into the red tuber skin Solanum tuberosum L. cv. Desiree. The anthocyanin content of the tuber peels of the transgenic lines was dramatically decreased by up to 70%. The possible production of flavonols in the peels of AS-DFR transgenic potatoes was discussed.
Antioxidant and Antidiabetic Activities of Eucommia ulmoides Bark
Qu, Guan-Zheng ; Heo, Seong-Il ; Wang, Myeong-Hyeon ;
Journal of Applied Biological Chemistry, volume 49, issue 3, 2006, Pages 82~85
Eucommia ulmoides bark extracts by cold water, boiling water, 100% EtOH, 70% EtOH, 100% MeOH, 70% MeOH and
were assayed for their medicinal effects. The antioxidant activity of the extracts ranged from
in the 1,1-diphenyl-2-picrylhydrazyl (DDPH) free radical-scavenging assay, and cold water extracts had the highest antioxidant activity.
extracts had the highest inhibitory effect on angiotensin I-converting enzyme (ACE) giving inhibition of up to 56.4% at a concentration of 1 mg/ml. Extracts in 100% EtOH had the greatest inhibitory effect on
), and 70% MeOH extracts had the greatest inhibitory effect on
). Taken together, these results provided the in vitro evidence on the ACE, amylase and glucosidase inhibitory actions of E. ulmoides bark that form the pharmacological basis for its antihypertensive and antidiabetic action.
Catalytic Hydrolysis of Phosphate Diesters as DNA Model with Tetranuclear Nickle (II) Complex
Sung, Nack-Do ; Kim, Tae-Young ;
Journal of Applied Biological Chemistry, volume 49, issue 3, 2006, Pages 86~89
The novel tetranuclear nickel (II) complex is a high rate accelerator in promoting hydrolysis of phosphate diesters. Nickel-bound bis-nitrophenyl phosphate (BNPP) can be
times more reactive than the unbound BNPP. The large rate of enhancements by the complex slightly under basic condition has shown high catalytic activity in phosphate diester cleavage. The bell-shaped pH-rate profile indicated that the nickel-oxide form of the tetranuclear complex or its kinetic equivalent was the active species for cleaving BNPP. The catalytic hydrolysis between tetranuclear nickel (II) complex and phosphate diester proceeds via the formation of bidentate coordination of the anionic phosphate to the Ni (II) atom. This reveals that the complex has the possibility as artificial nuclease.
Bactericidal Activity of Grapefruit (Citrus paradisl) Seed Extract-Based Disinfectant
Han, Jae-Hong ; Kim, Yong-Ung ; Kim, Ki-Yeon ; Hahm, Young-Tae ;
Journal of Applied Biological Chemistry, volume 49, issue 3, 2006, Pages 90~94
Bactericidal activity of grapefruit seed extract (GSE)-based disinfectant, as a safe disinfectant, was measured against five bacteria by Korean Food & Drug Administration (KFDA) dilution-neutralization method. GSE-based disinfectant showed a 99.9999% bactericidal activity against Escherichia coli ATCC 10536, Salmonella typhi ATCC 29629, Staphylococcus aureus ATCC 6538, Bacillus cereus ATCC 11778, and Listeria monocytogenes ATCC 1911 at the concentration of 2.15% GSE. It showed better bactericidal activity against Gram-negative bacteria of E. coli ATCC 10536 and S. typhi ATCC 29629 at lower concentration of GSE (0.43%). Based on the results, it was suggested that a possible bactericidal mechanism of GSE active ingredients was due to the abrupt osmotic shift during the bactericidal activity test by KFDA method.
Physico-chemical Properties of Giant Embryo Brown Rice (Keunnunbyeo)
Choi, In-Duck ; Kim, Deog-Su ; Son, Jong-Rok ; Yang, Chang-Inn ; Chun, Ji-Yeon ; Kim, Kee-Jong ;
Journal of Applied Biological Chemistry, volume 49, issue 3, 2006, Pages 95~100
Brown rice with a giant embryo (GE) was observed on the quality parameters of the enlargement of embryo, nutritional components, and physical properties, in comparison to normal embryo brown rice (NE). Also, the effects of germination on the quality parameters were examined. GE embryo was approximately 2.68 times larger than of NE rice. Total free sugars were significantly higher in GE rice (71.96 vs. 41.17 mg/100 g), and germinated rice increased in fructose, but decreased in sucrose and maltose. No significant difference in mineral contents was found in GE and NE rice and their germinated rice, whereas a significant increment was observed on reducing sugars and gamma-amino butyric acid (GABA) contents in GE rice. The lower water absorption index (WAI) of GE rice resulted in relatively lower pasting viscosity, whereas the increased WSI in germinated rice might be attributable to the significant increment of soluble components in GE rice.
Degradation of the Herbicide Butachlor by Laboratory-synthesized Nanoscale
in Batch Experiments
Kim, Hyang-Yeon ; Kim, In-Kyung ; Han, Tae-Ho ; Shim, Jae-Han ; Kim, In-Seon ;
Journal of Applied Biological Chemistry, volume 49, issue 3, 2006, Pages 101~105
Degradation of the herbicide butachlor was investigated using laboratory-synthesized zerovalent iron (
). The synthesized zerovalent iron was determined to be nanoscale powder by scanning electron microscopic analysis. To investigate degradation of butachlor using the synthesized nanoscale zerovalent iron, time-course batch experiments were conducted by treating the solution of butachlor formulation with the iron. More than 90% degradation of butachlor was observed by iron treatment within 24 h. The synthesized nanoscale zerovalent iron showed an increase in particle aggregation in the batch tests. Green rust formation and a pH drop in solutions were observed, suggesting that the oxidation of the iron occurred. When the iron was extracted with dichloromethane, a negligible concentration was found in the extract, suggesting that butachlor did not bind to the iron particles. GC/MS analysis detected the dechlorinated product as a major degradation product of butachlor in the solutions. The data indicate that laboratory-synthesized zerovalent iron functioned as a reductant to remove electron-withdrawing chlorine, giving the dechlorinated product.
Enhanced Production of L-Aspartate
by Nitrogen Source in Pseudomonas dacunhae
Kim, Dong-Chung ; Lee, Sung-Dong ; In, Man-Jin ;
Journal of Applied Biological Chemistry, volume 49, issue 3, 2006, Pages 106~109
Improvement of L-asparate
production from Pseudomonas dacunhae ATCC 21192 was attempted by optimizing fermentation conditions. Optimum carbon and nitrogen sources for cell growth and enzyme production were determined. L-Glutamate (2%) was the most suitable carbon source, and D-glucose, D-glycerol and fumarate repressed enzyme production. Yeast extract (2%) was the most effective as nitrogen source. A slight change of pH to 6.5 from medium pH resulted in a meaningful increase in the production of enzyme. The production of the enzyme was highly improved by using 2% yeast extract and 2% L-glutamate in culture media. Maximum L-asparate
activity reached up to over 24 U/mL-broth by 15 h flask fermentation.
Prolyl Endopeptidase Inhibitory Activity of 6-O-Palmitoyl L-Ascorbic Acid
Park, Yoon-Seok ; Paik, Young-Sook ;
Journal of Applied Biological Chemistry, volume 49, issue 3, 2006, Pages 110~113
Prolyl endopeptidase (PEP, EC 22.214.171.124, also referred to as prolyl oligopeptidase) degrades proline containing, biologically active neuropeptides such as vasopressin, substance P and thyrotropin-releasing hormone by cleaving peptide bonds on carboxyl side of prolyl residue within neuropeptides of less than 30 amino acids. Evaluation of PEP levels in postmortem brains of Alzheimer's disease patients revealed significant increases in PEP activity. Therefore, a specific PEP inhibitor can be a good candidate of drug against memory loss. Upon our examination for PEP inhibitory activity from micronutrients, ascorbic acid (vitamin C) showed small but significant PEP inhibition (13% PEP inhibition at
). Palmitic acid showed almost no PEP inhibition. However, 6-O-palmitoyl ascorbic acid (
) showed 70% PEP inhibition at
indicating that hydrophobic portion of the compound
may facilitate the inhibitory effect.
value of compound
. The primary and secondary Lineweaver Burk and Dixon plots for compound
indicated that it is a non-competitive inhibitor with inhibition constant (Ki) value of
Production of 5,7-dihydroxy, 3',4',5'-trimethoxyflavone from 5,7,3',4',5'-Pentahydroxyflavone Using two O-methyltransferases Expressed in Escherichia coli
Kim, Jeong-Ho ; Kim, Bong-Gyu ; Park, Young-Hee ; Han, Jin-Hee ; Lim, Yoong-Ho ; Ahn, Joong-Hoon ;
Journal of Applied Biological Chemistry, volume 49, issue 3, 2006, Pages 114~116