Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Applied Biological Chemistry
Journal Basic Information
Journal DOI :
The Korean Society for Applied Biological Chemisty
Editor in Chief :
Volume & Issues
Volume 52, Issue 4 - Dec 2009
Volume 52, Issue 3 - Sep 2009
Volume 52, Issue 2 - Jun 2009
Volume 52, Issue 1 - Mar 2009
Selecting the target year
Isolation of an Agarolytic Bacteria, Cellvibrio mixtus SC-22 and The Enzymatic Properties
Cha, Jeong-Ah ; Kim, Yoo-Jin ; Seo, Yung-Bum ; Yoon, Min-Ho ;
Journal of Applied Biological Chemistry, volume 52, issue 4, 2009, Pages 157~162
DOI : 10.3839/jabc.2009.027
An agar-liquefying bacteria (SC-22), which produces a diffusible agarase that caused agar softening around the colony was isolated from Daecheong lake in Korea. Chemotaxanomic and phylogenetic analyses based on 16S rRNA gene sequences revealed the strain was classified as Cellvibrio mixtus SC-22. The isolate SC-22 showed maximal extracellular agarase activity with 58.5 U/mL after 48 h cultivation in the presence of 0.2% agar. It was observed that the isolate produced two kinds of extracellular and three kinds of intracellular isoenzymes. The major agarase was purified from the culture filtrate of agarolytic bacteria by ammonium sulfate precipitation, anion exchange and gel filtration column chromatographic methods. The molecular mass of the purified enzyme was estimated to be 25 kDa by SDS-PAGE. The optimum pH and temperature of the purified enzyme were pH 7.0 and
, respectively. The agarase activity was activated by
ions while it was inhibited by
at 1 mM concentration. The predominant hydrolysis product of agarose by the enzyme was galactose and disaccharide on TLC, indicating the cleavage of
-1,4 linkage in a random manner. The enzyme showed high substrate specificity for only agar and agarose among various polysaccharides.
Isolation, Identification, and Characterization of Pichia guilliermondii K123-1 and Candida fermentati SI, Producing Isoflavone β-Glycosidase to Hydrolyze Isoflavone Glycoside Efficiently, from the Korean Traditional Soybean Paste
Kim, Won-Chan ; So, Jai-Hyun ; Kim, Sang-In ; Shin, Jae-Ho ; Song, Kyung-Sik ; Yu, Choon-Bal ; Kho, Yung-Hee ; Rhee, In-Koo ;
Journal of Applied Biological Chemistry, volume 52, issue 4, 2009, Pages 163~169
DOI : 10.3839/jabc.2009.028
A total of 155 microbial strains were isolated from the Korean traditional soybean paste based on their morphological features on the growth of agar plate. Among the isolated strains, a total of 28 strains were capable of hydrolyzing isoflavone glycoside to isoflavone aglycone efficiently in the soybean paste. Finally, two strains, K123-1 and SI, were selected because of their resistance to 15% NaCl and ability to convert isoflavone glycoside to isoflavone aglycone efficiently during the fermentation of soybean paste. The isolated strains K123-1 and SI were identified to be Pichia guilliermondii and Candida fermentati, respectively, using the partial 26S rDNA sequence analysis and phylogenic analysis. Pichia guilliermondii K123-1 and Candida fermentati SI converted daidzin to daidzein up to 96% and 95%, respectively, and genistin to genistein up to 92% when soybean pastes were fermented at
for 20 days with a single isolated strain. Pichia guilliermondii K123-1 and Candida fermentati SI were able to grow in the presence of 15% NaCl on both liquid medium and agar plate. We think that Pichia guilliermondii K123-1 and Candida fermentati SI might be one of good candidates for making functional soybean paste because they are isolated from the Korean traditional soybean paste and have a good ability to convert isoflavone glycosides to isoflavone aglycones and a high salt tolerance.
Immunoblotting Reactivity of Vitellogenin Antibodies against Native Vitellogenins and a Vitellogenin Protein Fragment Produced in E. coli
Ryu, Hyung-Seok ; Jang, In-Young ; Kim, Woo-Yeon ;
Journal of Applied Biological Chemistry, volume 52, issue 4, 2009, Pages 170~173
DOI : 10.3839/jabc.2009.029
Vitellogenin (Vtg) is found in the serum of both female and male fish that have been exposed to environmental endocrine disrupters or estrogen hormones, and is used as a biomarker for such contamination. In our current study antibodies raised against the purified flatfish Vtg were tested for their reactivity on immunoblots to flatfish and carp Vtg, and also to a Vtg protein fragment produced in E. coli. Polyclonal antibodies raised against purified flatfish Vtg reacted well with Vtg in the serum of flatfish and carp induced with
-estradiol, but not with the Vtg protein fragment produced in E. coli.
Evaluation and Comparison of Antioxidant Activities of Nineteen Commercial Carbonated Vinegar Liquors
Kim, Sung-Phil ; Nam, Seok-Hyun ;
Journal of Applied Biological Chemistry, volume 52, issue 4, 2009, Pages 174~179
DOI : 10.3839/jabc.2009.030
Biological functionality of nineteen commercially available carbonated vinegar liquors including wood vinegar liquor (WVL), bamboo vinegar liquor (BVL) and chaff vinegar liquor (CVL) were evaluated, focusing mainly on electron donating ability to DPPH radical, reducing power against ferricyanide (
), blockading ability to linoleic acid autoxidation and NO production from LPS-stimulated RAW264.7 cells plus cytotoxicity to RAW264.7 cells. The results showed that crude carbonated vinegar liquors, regardless of their source materials, have high capacity of antioxidation such as electron donating ability, reducing power, blockading ability to lipid peroxidation and NO production, as well as cell cytotoxicity. Refined carbonated vinegar liquors for skin care or bath showed significantly low cell cytotoxicity, however, overall antioxidant potencies were also low. Especially, these carbonated vinegar liquors revealed low levels of inhibition for NO production deeply involved in inflammation. Among nineteen carbonated vinegar liquors examined, chaff vinegar liquor was observed to be the most potent carbonated vinegar liquor with high antioxidant activities together with low cytotoxicity to mammalian cells.
Antiproliferative Effect of Extracts, Fractions and Compound from Vitex rotundifolia on Human Cancer Cells
Kim, You-Ah ; Lee, Jung-Im ; Kim, Hae-Jin ; Kong, Chang-Suk ; Nam, Taek-Jeong ; Seo, Young-Wan ;
Journal of Applied Biological Chemistry, volume 52, issue 4, 2009, Pages 180~186
DOI : 10.3839/jabc.2009.031
Whole plants of Vitex rotundifolia were extracted for 2 days with methylene chloride (
) followed by extraction of the residue for an additional 2 days. The same procedure was also applied using methanol (MeOH). The two crude extracts were combined and partitioned between
. The organic layer was further partitioned between n-hexane and 85% aq. MeOH, and the aqueous layer was also further fractionated with n-BuOH and
, successively. From the 85% aq. MeOH fraction, one compound was isolated through the repeated HPLC. According to the results of physicochemical data including NMR and MS, the chemical structure of the compound was determined as artemetin (1). The antiproliferative effects of the crude extracts, fractions, and compound against HT1080, AGS, MCF-7 and HT-29 human cancer cells were compared with the control by using MTT assay. In the comparative analysis, the 85% aq. MeOH fraction exhibited the strongest antiproliferative effects on human cancer cell lines in a dose-dependent manner (p<0.05). In addition, exposure of compound 1 isolated from 85% aq. MeOH fraction led to strong antiproliferative effect in HT1080 cancer cell lines. These results suggest that the extracts and compound isolated from V. rotundifolia may be used as potential chemopreventive and chemotherapeutic agents.
ACAT (Acyl-CoA:cholesterol Acyltransferase) Inhibitory Effect and Quantification of Pyranocurmarin in Different Parts of Angelica gigas Nakai
Kim, Geum-Soog ; Park, Chun-Geun ; Jeong, Tae-Sook ; Cha, Seon-Woo ; Baek, Nam-In ; Song, Kyung-Sik ;
Journal of Applied Biological Chemistry, volume 52, issue 4, 2009, Pages 187~194
DOI : 10.3839/jabc.2009.032
Two pyranocoumarin constituents have been isolated from Angelica gigas and were identified as decursinol angelate (1) and decursin (2) by means of NMR analysis, respectively. Human acyl-CoA:cholesterol acyltransferase (hACAT) inhibitory activity of decursinol angelate (1) and decursin (2) was evaluated. Decursin (2) showed significantly inhibitory activity against hACAT1 and hACAT2 with
value of 137 and
, respectively, whereas decursinol angelate (1) exhibited weak ACAT inhibitory activity. These results suggested that decusin from A. gigas might be effective for the prevention and the treatment of hypercholesterolemia or atherosclerosis by inhibitory effect on hACAT. The contents of decursinol angelate (1) and decursin (2) were analyzed in various parts of A. gigas including flower, seed, leaf and root using LC/MS/MS (ESI, positive ion mode, MRM mode). The content of decursinol angelate was increased in order of flower, seed, leaf, and root and decursin content was increased in order of flower, seed, leaf, and root. It was expected that unused parts including leaf and flower of A. gigas might be useful as new functional sources by their high contents of decursin and decursinol angelate.
Surfactants Effective to the Control of Cucumber Powdery Mildew
Yu, Ju-Hyun ; Choi, Gyung-Ja ; Lim, He-Kyoung ; Kim, Heung-Tae ;
Journal of Applied Biological Chemistry, volume 52, issue 4, 2009, Pages 195~199
DOI : 10.3839/jabc.2009.033
To select antifungal surfactants, control efficacy of various nonionic and anionic surfactants on cucumber powdery mildew was evaluated under greenhouse conditions. Among 14 surfactants, pentaetylene glycol monododecyl ether (
), mixture of heptaethylene glycol monodecyl ether and heptaethylene monododecyl ether, and heptaethylene glycol mono-9-octadecenyl ether effectively reduced the development of powdery mildew on cucumber plants. Among the surfactants,
gave the best control efficacy on the disease and did not show phytotoxic response in cucumber plants. Whereas, fenarimol at a recommended rate (31.3 mg/L) showed less control activity than the surfactant (1,000 mg/L). In addition, mixtures of the surfactant and DBEDC, a protective fungicide, showed high control efficacies against powdery mildews of cucumber and strawberry by additive effect in greenhouse tests.
Change of Biological Activity of Melon (Cucumis melo L.) according to Frozen Storage Period
Cho, Jun-Gu ; Youn, Sun-Joo ; Lee, Eun-Tag ; Kim, Tae-Wan ; Kwoen, Dae-Jun ;
Journal of Applied Biological Chemistry, volume 52, issue 4, 2009, Pages 200~204
DOI : 10.3839/jabc.2009.034
The change of biological activities of melon were investigated during frozen storage. The total phenolic concentrations in melon juice and water extract were 296.25 and
, respectively. The total flavonoid contents in melon juice and water extract were 20.83 and
, respectively. Antioxidant activities of melon juice and water extract were determined. The DPPH of water extract of melon (85.84%) was higher than the melon juice (60.58%). ABTS of melon juice and water extract were 94.50 and 99.30%, respectively. SOD-like activity and xanthine oxidase inhibitory activity of melon of water extracts were higher than those of melon juice.
-Glucosidase inhibitory activity of melon juice and water extract were 22.42 and 23.43%, respectively. The changes in the antioxidant activity of melon was insignificant until 6 months of frozen storage. Therefore, it was expected that frozen storage of melon was useful preservation expedient for consistent supply of raw materials.
Quality Analysis on the Size and the Preparation Method of Meju for the Preparation of Korean Traditional Soy Sauce (Kanjang)
Lee, Jong-Gu ; Kwon, Kwang-Il ; Choung, Myoung-Gun ; Kwon, O-Jun ; Choi, Ji-Young ; Im, Moo-Hyeog ;
Journal of Applied Biological Chemistry, volume 52, issue 4, 2009, Pages 205~211
DOI : 10.3839/jabc.2009.035
This study was carried out to acquire basic data for industrial production of Korean traditional kanjang (soy sauce). Five types of meju,
(made a hole
1.5 cm) were prepared. The temperature and humidity of meju preparation were
and 40~50% respectively. The smaller size of meju, the lower free amino acid and non-volatile organic acid content of that. And, two types of meju, conventional method (CM-meju, the temperature and humidity were prepared at
and 40~50% of relative humidity) and improved method(IM-meju, the temperature and humidity were prepared at
and 80~90% of relative humidity) for kanjang production were prepared. There was no difference of total nitrogen content and soluble nitrogen content in the size of meju. In total free amino acid content and total free sugar content, IM-meju was the higher than CM-meju. So, the quality of IM-meju was better than that of CM-meju.
Suppression Effect of Curcuma longa Rhizome-Derived Components against Nitric Oxide Synthase
Lee, Hoi-Seon ;
Journal of Applied Biological Chemistry, volume 52, issue 4, 2009, Pages 212~215
DOI : 10.3839/jabc.2009.036
The inhibitory effects of Curcuma longa rhizome-derived materials against nitric oxide (NO) production were assessed. The inhibitory effect (57%) on NO production was evidenced by the methanol extract of C. longa at
. In the fractionation of the methanol extract, the ethyl acetate fraction evidenced an inhibitory effect greater than 62.1% at
. The active constituent was identified as curcumin. Curcumin exerted potent inhibitory effects of 78.7 and 65.7% at concentrations of 1 and
, respectively. Furthermore, the inhibitory effect of ar-turmerone was measured as 31.3 and 15.8% at 1 and
, respectively. The iNOS expression-suppressive effects of curcumin were assessed via western blot analysis. Our results suggest that curcumin and ar-turmerone may prove useful in the development of new types of NO inhibitors.
Antioxidant and Aldo-keto Reductase Family 1 B10 Inhibition Activities of Korean Local Plant Extracts
Pan, Cheol-Ho ; Lee, Joo-Young ; Song, Dae-Geun ; Kim, Jong-Hwan ; Ahn, Soo-Young ; Bae, Deok-Sung ; Kim, Young-Hoon ; Lee, Jae-Kwon ;
Journal of Applied Biological Chemistry, volume 52, issue 4, 2009, Pages 216~220
DOI : 10.3839/jabc.2009.037
Aldo-keto reductase family 1 B10 (AKR1B10) has been considered as a potential cancer therapeutic target. Ethanol extracts prepared from 82 Korean local plants were examined for their antioxidant activity and inhibitory effects on recombinant human AKR1B10 (rhAKR1B10) in vitro. 21 extracts showed more than 80% of ABTS radical scavenging activity at
and 11 extracts inhibited more than 50% of rhAKR1B10 activity at
. Especially, 9 extracts showed potent inhibition on rhAKR1B10 activity compared with positive control tetramethylene glutaric acid.
Inhibitory Effect of Acetylshikonin from Roots of Lithospermum erythrorhizon on LDL Oxidation and FPTase Activity
Kim, Geum-Soog ; Jeong, Tae-Sook ; Kwon, Byoung-Mok ; Kim, Young-Ok ; Cha, Seon-Woo ; Song, Kyung-Sik ; Bek, Nam-In ;
Journal of Applied Biological Chemistry, volume 52, issue 4, 2009, Pages 221~225
DOI : 10.3839/jabc.2009.038
Lithospermum erythrorhizon has been known well as one of traditional medicine for fever reduction, detoxication, and blood circulation improvement. This study was carried out to isolate biological active compounds from roots of Lithospermum erythrorhizon and to investigate their low density lipoprotein (LDL) antioxidant and anticancer activities. The hexane extract of Lithospermi Radix has been separated on silica gel chromatography and a naphthoquinone pigment compound 1 has been isolated. The structure of the compound 1 has been identified by spectroscopic technique, including MS and NMR, as acetylshikonin (1). Acetylshikonin showed significantly inhibitory activity on
-induced human LDL oxidation with
and obvious anticancer effect by inhibiting farnesyl:protein transferase (FPTase) activity with
, which suggested that acetylshikonin might be useful for the treatment of atherosclerosis and cancer.