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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
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Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 53, Issue 4 - Dec 2010
Volume 53, Issue 3 - Sep 2010
Volume 53, Issue 2 - Jun 2010
Volume 53, Issue 1 - Mar 2010
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Construction of Xylose-Inducible Expression Vector Using xylA Promoter of Escherichia coli
Kim, Hyun-Ho ; So, Jai-Hyun ; Rhee, In-Koo ;
Journal of Applied Biological Chemistry, volume 53, issue 1, 2010, Pages 1~7
DOI : 10.3839/jabc.2010.001
xylA promoter is a major promoter in xylose operon of Escherichia coli. xylA promoter is sufficient as the promoter for the construction of new expression vector because this promoter was tightly controlled and induced by the addition of xylose. For the construction of xylose-inducible expression vector, 600 bp of xylA promoter was ligated between AatII and HindIII of pUC18, named pXA600. In order to investigate the effect of XylR protein encoded by xylR gene on the xylA promoter, 1,988 bp of xylR gene including its promoter was ligated into downstream of multiple cloning site to the opposite direction of xylA promoter in pXA600, named pXAR600. For the measurement of expression level, 3,048 bp of lacZ structural gene was fused into xylA promoter in both plasmids pXA600 and pXAR600 as a reporter gene, named pXA600-lacZ and pXAR600-lacZ, respectively. The
-galactosidase activity of pXA600-lacZ and pXAR600-lacZ in E. coli JM109 was determined to be 1,641 and 2,304 unit by the induction with xylose in LB medium, respectively. The
-galactosidase activity of pXAR600-lacZ/JM109 was about 1.4 times higher by the induction with xylose than that of pXA600-lacZ/JM109. The
-galactosidase activity of pXA600-lacZ and pXAR600-lacZ in E.coli JM109 showed 6,282 and 9,320 unit by the induction with xylose in DM minimal medium, respectively. A regulator, xylR protein works as an activator for the gene expression by the addition of xylose in the xylose-inducible vectors because the level of gene expression in pXA600 is increased by the insertion of xylR gene into the same vector. The xynA gene of Streptomyces thermocyaneoviolaceus cloned in pXA600 and pXAR600 was successfully expressed in E. coli BLR(DE3). As a result, plasmids pXA600 and pXAR600 using xylA promoter are sufficient as new expression system to produce a foreign protein in E. coli.
Antioxidant Activity of Hawthorn Fruit in vitro
Li, Chunmei ; Han, Woong ; Wang, Myeong-Hyeon ;
Journal of Applied Biological Chemistry, volume 53, issue 1, 2010, Pages 8~12
DOI : 10.3839/jabc.2010.002
The antioxidant activity of hawthorn fruit (Crataegus pinnatifida Bunge var. typica Schneider) extracts was investigated by several in vitro antioxidants properties, including DPPH free radical scavenging activity, total phenolic and flavonoid contents, hydroxyl radical scavenging activity, reducing power activity, iron-chelating capacity and nitrite scavenging activity. Among the extracts in this study, the 70% EtOH extract showed higher antioxidant activity than the others. The
value of DPPH free radical scavenging activity was
. Furthermore, the 70% EtOH extract also showed significantly high total phenolic and flavonoids contents and reducing power activity. However, the MeOH extract exhibited stronger effects on hydroxyl radical scavenging activity, iron-chelating capacity and nitrite scavenging activity. All the results implicated that, the hawthorn fruit may has the available potential to be utilize as a potential source of natural antioxidant.
Anti-oxidant and Anti-microbial Activities of Seungmakalgeuntang
Lee, Jin-Young ; Bae, Ho-Jung ; Park, Tae-Soon ; Kim, Tae-Wan ; Moon, Doo-Hwan ; Kwon, O-Jun ; Son, Jun-Ho ; Lee, Chang-Eon ; Park, Gun-Hye ; Kim, Han-Hyuk ; An, Bong-Jeun ;
Journal of Applied Biological Chemistry, volume 53, issue 1, 2010, Pages 13~20
DOI : 10.3839/jabc.2010.003
Biological activities such as anti-oxidative and anti-microbial of the Seungmakalgeuntang, a traditional prescription, were evaluated. The electron donating ability of water, ethanol, supercritical fluid and 1,3-butylene glycol extract of Seungmakalgeuntang showed more than 50% at a 100 ppm concentration. At a 1000 ppm concentration, the superoxide dismutase-like activities of ethanol and supercritical fluid extract of Seungmakalgeuntang showed less than 50%. xanthine oxidase inhibition effect of the supercritical fluid extract showed more than 70% at a 1,000 ppm concentration, which was higher than vitamin C. From the measurement on lipid oxidation, the
chelating abilities of the supercritical fluid extract of Seungmakalgeuntang was more than 60% at a 100 ppm concentration. Also the
chelating abilities of supercritical fluid extract Seungmkalgeuntang was showed more than 60% at a 500 ppm concentration. Clear zones formed by sample against the human skin-resident microflora such as Staphylococcus epidermidis, Staphylococcus aureus and Propionibacterium acne of ethanol and supercritical fluid extract of Seungmakalgeuntang showed the highest among all the extracts tested using a 4mg/disc. The minimum inhibitory concentration (MIC) against both S. epidermidis and S. aureus showed 2,500 ppm in the extract of the supercritical fluid.
Insecticidal activity of the crude extract and its fractions of Custard apple (Annona reticulata L.)
Shin, Suk-Hyun ; Choi, Geun-Hyoung ; Choi, Dal-Sun ; Kwon, Oh-Kyung ; Im, Geon-Jae ; Park, Jae-Up ; Choi, Byung-Ryul ; Kim, Tae-Wan ; Kim, Jin-Hyo ;
Journal of Applied Biological Chemistry, volume 53, issue 1, 2010, Pages 21~24
DOI : 10.3839/jabc.2010.004
In recent years, many agricultural scientists are studying on eco-friendly farming methods. Among of lots of the methods, the natural insecticides are highly motivated and interested due to their safety and biodegradable issues, and readily available source of bioinsecticides. In this study, the crude extract of custard apple (Annona reticulata L.) seed and its three fractions which were separated based on polarity indexes were examined for their insecticidal activities against Myzus persicae Sulzer and Nilaparvata lugens S.. The crude extract (Aceton/MeOH) showed strong insecticidal activities against both insects at 3.00 mg/mL (
=0.45mg/mL for M. persicae S. and 1.42 mg/mL for N. lugens S.). Furthermore, simple fractionation with hexane, chloroform, and water lead to increase three-folds insecticidal activity on chloroform fraction (
=0.13mg/mL for M. persicae S. and 1.14 mg/mL for N. lugens S.). The results suggest that A. reticulata extracts might be used to control for M. persicae effectively.
Simultaneous Analytical Method for the Neomycin, Gentamicin Residues in Seafood
Hong, Young-Min ; Lee, Seok-Ki ; Kim, Hyoung-Ah ; Hwang, Yu-Kyung ;
Journal of Applied Biological Chemistry, volume 53, issue 1, 2010, Pages 25~30
DOI : 10.3839/jabc.2010.005
This paper describes a simultaneous method for the determination of two aminoglycosides (neomycin and gentamicin) using solid phase extraction followed by liquid chromatograph-mass spectrometry. The extract was applied to an WCX and HLB solid phase extraction cartridge. The cartridges were washed with water and methanol, and analytes were eluted with TCA buffer-acetonitrile mixture. The aminoglycosides were separated by ion-pairing reversed phase mode prior to ESI-LC/MS. Under the conditions applied neomycin was almost separated from all the gentamicin compounds. No interfering peaks from endogenous compounds of matrix were noted at the elution position of the analytes. Recoveries of neomycin fortified at levels of 0.25, 0.5, 1.0 and 2.0 mg/kg seafood samples ranged from 92 to 115%. Recoveries of gentamycin fortified at levels of 0.05, 0.1, 0.2, 0.4 mg/kg seafood samples ranged from 99 to 116%. Method detection limits in four seafood sample matrices were between 0.002 and 0.033 mg/kg.
Antioxidant and Quinone Reductase Inductive Activities of Various Organs of Pepper
Ku, Kang-Mo ; Kang, Young-Hwa ;
Journal of Applied Biological Chemistry, volume 53, issue 1, 2010, Pages 31~36
DOI : 10.3839/jabc.2010.006
We analyzed antioxidant and quinone reductase (QR) inductive activities of various organs of pepper for utilizing by-product of them. Peppers were separated into fruits, roots, stems, and leaves and extracted with methanol for the analysis. As a result, pepper leaves showed higher phenol content than other organs. Using the DPPH assay, there was not considerably different activity depending on pepper organs, but pepper leaves showed significantly higher antioxidant activity using the ABTS assay. In FTC and TBA assay, stems and leaves showed significantly higher lipidperoxidation inhibitory activity. In QR inductive assay, pepper tissues showed different QR inductive activity: leaves>roots>>stems>fruits. In addition, pepper leaves showed highest antiproliferation activity on hepa1c1c7 among pepper tissues in
. These results indicate that pepper leaves have high potential to be a good functional food material due to high QR inductive and antioxidant activities.
The Chemical Quality Properties during Processing Treatment of Brown Rice Varieties with Different Amylose Content
Lee, Jong-Gu ; Kwon, Kwang-Il ; Choi, Ji-Young ; Choi, Jong-Dong ; Choung, Myoung-Gun ; Im, Moo-Hyeog ;
Journal of Applied Biological Chemistry, volume 53, issue 1, 2010, Pages 37~43
DOI : 10.3839/jabc.2010.007
This study was carried out to determine the chemical quality properties during processing treatment using the brown rice varieties with different amylose content. It was investigated using Hwaseonchalbyeo, Baegjinjubyeo, Ilpumbyeo and Goamibyeo varieties. As for the chemical properties of fried brown rice, phenolic compounds increased by the frying treatment. The component of aroma after the frying treatment, it showed that aldehydes sorts of benzaldehyde, phenylmethanal had the highest content. The content of vitamin B group during parboiling treatment and frying treatment decreased as a whole than the non-treatment brown rice. The higher the content of amylose was, the more the content of vitamin
increased. The higher the content of amylose was, the more the content of vitamin
and mineral contents decreased. The component of mineral during frying treatment, it showed that Mg had the highest content. The higher the content of amylose increased, the more the blue value increased. The 2,2-diphenyl-
-picryl-hydrazyl (DPPH) after the frying treatment was relatively increased.
Anti-obesity Effects of Tae-Um-Jo-Wee-Tang and Do-Dam-Tang in Female Rats with Diet-induced Obesity
Park, Sun-Min ; Ahn, Il-Sung ; Kim, Da-Sol ; Kang, Sun-A ; Kwon, Dae-Young ; Yang, Hye-Jeong ;
Journal of Applied Biological Chemistry, volume 53, issue 1, 2010, Pages 44~50
DOI : 10.3839/jabc.2010.008
Tae-Um-Jo-wee-Tang (TUJWT) and Do-Dam-Tang (DDT) have been used as an anti-obesity herbal medicine but their effect and mechanism of action have not been studied. We investigated the effects of TUJWT and DDT on energy and glucose homeostasis using Sprague Dawley female rats with diet-induced obesity. The mechanisms of action of TUJWT and DDT were studied whether they had anti-obesity effects. Rats fed a high-fat diet were divided into 3 groups: rats in each group received 2 g water extracts of modified TUJWT and DDT, or 2 g cellulose per kg body weight (a negative control) on a daily basis. A further group was fed a low-fat diet as a positive control. We found that DDT significantly decreased body weight and body fat (mesenteric fat and retroperitoneal fat) more than the control. This decrease was due to the reduction in energy intake but no changes of energy expenditure. However, DDT increased fat oxidation as a major energy source than the control. In addition, modified TUJWT and DDT improved glucose tolerance without changing serum insulin levels during an oral glucose tolerance test. In conclusion, DDT have a better anti-obesity effect than TUJWT by decreasing energy intake in female rats with diet-induced obesity. It also improves glucose tolerance.
Production of a Fermented Korean Pear Puree using a New Strain Leuconostoc mesenteroides KACC 91495P Isolated from Kimchi
In, Man-Jin ; Kim, Hye-Min ; Jin, Hea-Jin ; Kim, Dong-Chung ; Oh, Nam-Soon ; Chae, Hee-Jeong ;
Journal of Applied Biological Chemistry, volume 53, issue 1, 2010, Pages 51~55
DOI : 10.3839/jabc.2010.009
A lactic acid bacterial strain showing fast growth and high acid production in Korean pear puree was isolated from Kimchi. This strain was analyzed by API 50 CHL kit and 16S rRNA sequencing analysis and identified as Leuconostoc (Ln.) mesenteroides KACC 91495P. Korean pear puree was fermented using Ln. mesenteroides KACC 91495P strain at
for 18 h. The changes of pH, titratable acidity and viable cell number during fermentation were investigated. The pH and titratable acidity were reached to pH 3.86 and 1.09% after 18 h fermentation, respectively. The viable cell population of Ln. mesenteroides KACC 91495P was rapidly increased to
during the 9 h of cultivation. The contents of lactic acid, acetic acid and malic acid were determined to be 0.213, 0.259, and 0.217% after 18 h fermentation, respectively. The content of polyphenolic compounds, known as antioxidants, in pear puree were enhanced by Ln. mesenteroides KACC 91495P cultivation. The level of total polyphenolic compounds was increased to around 140% of initial concentration. When the fermented pear puree was kept at
, pH, titratable acidity and number of viable cells population were nearly maintained for 13 days.
Preparation of Mulberry Leaves Tea and Its Quality Characteristics
Kim, Dong-Chung ; In, Man-Jin ; Chae, Hee-Jeong ;
Journal of Applied Biological Chemistry, volume 53, issue 1, 2010, Pages 56~59
DOI : 10.3839/jabc.2010.010
An optimal manufacturing process was established for the mass-production of mulberry leaves tea. The produced tea contained abundant dietary fibers,
-carotenes, and calcium. Also the polyphenol content and antioxidant activity of mulberry leaves tea were higher than those of simply dried mulberry leaves. These results imply that this tea manufacturing process is effective to utilize the useful components of mulberry leaves.
Cloning and Characterization of Isoflavone synthase from Lespedeza bicolor
Kim, Bong-Gyu ;
Journal of Applied Biological Chemistry, volume 53, issue 1, 2010, Pages 60~64
DOI : 10.3839/jabc.2010.011
None of genes from Lespedeza bicolor involved in isoflavonoid biosynthesis have been biochemically characterized. An isoflavone synthase from Lespedeza bicolor was cloned. To verify its catalytic activity, a fusion protein of LbIFS with P450 reductase from rice was made. Using this construct, production of isoflavone from flavanone was confirmed.