Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Applied Biological Chemistry
Journal Basic Information
Journal DOI :
The Korean Society for Applied Biological Chemisty
Editor in Chief :
Volume & Issues
Volume 53, Issue 4 - Dec 2010
Volume 53, Issue 3 - Sep 2010
Volume 53, Issue 2 - Jun 2010
Volume 53, Issue 1 - Mar 2010
Selecting the target year
Production of Medium-chain Fatty Acids in Brassica napus by Biotechnology
Roh, Kyung-Hee ; Lee, Ki-Jong ; Park, Jong-Sug ; Kim, Hyun-Uk ; Lee, Kyeong-Ryeol ; Kim, Jong-Bum ;
Journal of Applied Biological Chemistry, volume 53, issue 2, 2010, Pages 65~70
DOI : 10.3839/jabc.2010.012
Medium-chain fatty acids (MCFA) are composed of 8-12 carbon atoms, and are found in coconut, cuphea, and palm kernel oil. MCFA were introduced into clinical nutrition in the 1950s for dietary treatment of malabsorption syndromes because of their rapid absorption and solubility. Recently, MCFA have been applied to Gastrointestinal Permeation Enhancement Technology (GIPET), which is one of the most important parts in drug delivery system in therapeutics. Therefore, to accumulate the MCFA in seed oil of rapeseed, much effort has been conducted by classical or molecular breeding. Laurate can be successfully accumulated up to 60 mol% in the seed oil of rapeseed by the expression of bay thioesterase (Uc FatB1) alone or crossed with a line over-expressing the coconut lysophosphatidic acid acyltransferase (LPAAT) under the control of a napin seed-storage protein promoter. Also, caprylate and caprate were obtained 7 mol% and 29 mol%, respectively, from plants over-expressing of the medium-chain specific thioesterase (Ch FatB2) alone or together with the chain-length-specific condensing enzyme (Ch KASIV). Despite the success of some research in utilizing parallel classical and molecular breeding to produce MCFA, commercially available seed oils have for the most part, not been realized. Recent research in the field of developing MCFA-enriched transgenic plants has established that there is no single rate-limiting step in the production of the target fatty acids. The purpose of this article is to review some of the recent progress in understanding the mechanism and regulation of MCFA production in seed oil of rapeseed.
Characterization of the Bacillus licheniformis WL-12 Mannanase from a Recombinant Escherichia coli
Yoon, Ki-Hong ;
Journal of Applied Biological Chemistry, volume 53, issue 2, 2010, Pages 71~76
DOI : 10.3839/jabc.2010.013
A gene encoding the mannanase of Bacillus licheniformis WL-12, which had been isolated from Korean soybean paste, was cloned into Escherichia coli and nucleotide sequence of the mannanase gene was subsequently determined. The mannanase gene consisted of 1,080 nucleotides encoding a polypeptide of 360 amino acid residues. The deduced amino acid sequence was identical to that of putative mannanase from B. liceniformis DSM13 belonging to GH family 26. The mannanase was partially purified from cell-free extract of the recombinant Escherichia coli carrying a WL-12 mannanase gene by ammonium sulfate fractionation and DEAE-Sepharose column chromatography. Optimal conditions for the partially purified enzyme occurred at pH 6.0 and
. The enzyme showed higher activity on locust bean gum (LBG) galactomannan and konjac glucomannan than on guar gum galactomannan. The predominant products resulting from the mannanase hydrolysis were mannose, mannobiose and mannotriose for LBG or mannooligosaccharides. The enzyme could hydrolyze mannooligosaccharides larger than mannobiose.
Sterols from the Seed of Cowpea (Vigna sinensis K.)
Cui, En-Ji ; Park, Hee-Jung ; Wu, Qian ; Chung, In-Sik ; Kim, Ji-Young ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 53, issue 2, 2010, Pages 77~81
DOI : 10.3839/jabc.2010.014
The seed of cowpea (Vigna sinensis K.) was extracted with 80% aqueous methanol (MeOH). And the concentrated extract was partitioned with ethyl acetate (EtOAc), n-butanol (n-BuOH) and
, successively. The repeated silica gel and octadecyl silica gel (ODS) column chromatographic separations for the EtOAc and n-BuOH fractions led to isolation of four sterols. And the chemical structures of the compounds were determined as a mixture of stigmasterol and
-sitosterol with the ratio of 4 to 3 (1), 7-ketositosterol (2), and stigmasterol 3-O-
D-glucopyranoside (3) from the interpretation of spectroscopic data including nuclear magnetic resonance (NMR) spectrum metric, mass (MS) spectrum metric and infrared (IR) spectroscope. This study reports the first isolation of
-sitosterol, 7-ketositosterol, and stigmasterol 3-O-
-D-glucopyranoside from the seed of Vigna sinensis K. In addition, compound 2, 7-ketositosterol, is rarely occurred in natural source including plant.
Antioxidant Activity of Branches of Fuyu and Nishimurawase Persimmon Trees
You, Dong-Hyun ; Jang, In-Cheol ; Seo, Go-Un ; Kim, Su-Jung ; Yang, Eun-Kyung ; Ahn, Gwang-Hwan ; Matsushita, Yasuyuki ; Fukushima, Kazuhiko ; Lee, Seung-Cheol ;
Journal of Applied Biological Chemistry, volume 53, issue 2, 2010, Pages 82~86
DOI : 10.3839/jabc.2010.015
The antioxidant activities of two persimmon (Diospyros kaki T.) branches were evaluated. After preparing acetone, 70% acetone, and methanol extracts from Fuyu and Nishimurawase persimmon branches, DPPH radical scavenging activity (RSA), ABTS RSA, reducing power (RP), and total phenol contents (TPC) were determined. The acetone extracts of Fuyu and Nishimurawase showed higher antioxidant properties than the other extracts. The highest TPC (22.48 mg/g), DPPH RSA (
), ABTS RSA (
), and RP (
) were found in acetone extract of Fuyu, while the lowest antioxidant activities were in 70% acetone extract of Nishimurawase. The results suggest that sweet persimmon branches exhibit considerable antioxidant activity.
Phenolic Compounds Content and Tyrosinase Inhibitory Effect of Unripe Apple Extracts
Choi, Sang-Yoon ; Kim, Sung-Soo ; Lee, Yeon-Mi ; Lee, Bog-Hieu ; Han, Chan-Kyu ;
Journal of Applied Biological Chemistry, volume 53, issue 2, 2010, Pages 87~90
DOI : 10.3839/jabc.2010.016
This study consisted of a quantitative analysis of five phenolic ingredients in differently sized unripe apple extracts, and their tyrosinase inhibitory effects were examined. In the HPLC analysis of phenolic ingredients, small (
per one) unripe apple extracts were observed to have significantly higher quercetin content than larger (
per one) unripe apple and ripe apple extracts. The amount of catechin, epicatechin, p-coumaric acid and chlorogenic acid contents were similar in both the small and large unripe apple extracts. For the results of the tyrosinase assay, small unripe apple extracts provided a potent tyrosinase inhibitory effect, showing 89.2% at 1000 ppm. The tyrosinase inhibitory effects of large unripe apple and ripe apple extracts were weaker than those of the small unripe apple extract. These results suggest that the small unripe apple extract could be useful for de-pigmenting material, while quercetin could be responsible for the potent tyrosinase inhibitory properties of small unripe apple extracts.
Antioxidative activity and Angiotensin Converting Enzyme Inhibitory activity of Fermented Medical Plants (DeulBit) and Its Modulatory Effects of Nitric Oxide Production
Cho, Eun-Kyung ; Gal, Sang-Wan ; Choi, Young-Ju ;
Journal of Applied Biological Chemistry, volume 53, issue 2, 2010, Pages 91~98
DOI : 10.3839/jabc.2010.017
This study was aimed to investigate the contents of flavonoids and the biological activity of fermented beverage of medical plants, DeulBit (DB). 50 g of Cassia semen (Cassia tora L.), 50 g of Omija (Schisandra chinensis Baillon.), 50 g of Gugija (Lycium chinense Mill), 50g of Menthae herba, 75 g of Chrysanthemum indicum Linne, 25 g of Dioscorea batatas, 5 g of Lindera obtusiloba Blume, 150 g of Polygonatum odoratum, 25 g of Glycyrrhiza uralensis, 25 g of Acanthopanacis cortex, 100 g of green tea (Camellia sinensis), and 100 g of Laminaria japonica was fermented with sucrose (
.) and 0.5% of deep sea water in 10 L of distilled water for six months at room temperature. Total flavonoids contents of DB was calculated to
and antioxidative activity of DB was measured by using DPPH radical scavenging and SOD-like activity. DPPH radical scavenging and SOD-like activity of DB was 96% and 29% at 100% of DB, respectively. In addition, DB indicated about 88% and 66% of the xanthine oxidase and angiotensin converting enzyme inhibitory activities at 1% and 10% of DB, respectively and showed fibrinolytic activity. Nitric oxide (NO) synthesis was increased to 15 times by addition of DB. In addition, NO productions of the macrophages RAW264.7 cells stimulated with lipopolysaccharide (LPS) were reduced to 40.4% by addition of DB. These results suggested that DB is significant role for antioxidative and fibrinolytic activity, and have the strong xanthine oxidase and angiotensin converting enzyme inhibitory activities.
The Influence of the Substituents on the Benzo Ring for Antioxidant Activity of 4-Methyl-2H-benzopyran-2-one Analogues
Choi, Won-Seok ; Lee, Jae-Whang ; Cho, Yun-Gi ; Sung, Nack-Do ;
Journal of Applied Biological Chemistry, volume 53, issue 2, 2010, Pages 99~104
DOI : 10.3839/jabc.2010.018
The influences on antioxidant activities of the substituents (
) on benzo ring in 4-Methyl-2H-benzopyran-2-one analogues (1-23) were discussed quantitatively using three dimensional quantitative structure-activity relationships (3D-QSARs: Comparative molecular field analyses (CoMFA) and Comparative molecular similarity indice analyses (CoMSIA)) methods. The statistical qualities of CoMSIA models were better than those of CoMFA models and the CoMSIA 2 model was optimized model (
=0.979). Also, the contribution ratios (%) of the optimized CoMSIA 2 model were H-bond donor field 43.5%, electrostatic field 41.8% and steric field 14.7% so that the antioxidant activity exhibited a strong correlation with H-bond donor and electrostatic factor of molecules. From the analytical results of the CoMSIA contour maps, if the positive charge favor group and H-bond donor disfavor group were placed in the
positions on the benzo ring, it was predicted that the groups would raised the antioxidant activity.
Effect of Agarase Signal Peptide from Agarivorans albus YKW-34 on Protein Secretion in Escherichia coli
Lee, Joo-Young ; Song, Dae-Geun ; Son, Jin-Ki ; Pan, Cheol-Ho ;
Journal of Applied Biological Chemistry, volume 53, issue 2, 2010, Pages 105~107
DOI : 10.3839/jabc.2010.019
To overcome the limitation of E. coli expression system such as inclusion body formation and disulfide bond failure, we tried to express the heterologous protein as a secreted form. We adopted agarase signal peptide (ASP; 23 amino acid residues) from Agarivorans albus YKW-34 which is one of marine bacteia. When we used ASP to express
-agarase, about 42% activity was detected in media.
Synthesis of Salens and Their Cobalt Complexes from Meso-1,2-Diamine and Their Anti-Cancer Property
Koh, Dong-Soo ;
Journal of Applied Biological Chemistry, volume 53, issue 2, 2010, Pages 108~111
DOI : 10.3839/jabc.2010.020
New salens (3) and their Cobalt complexes (4) were prepared from meso-1,2-bis(ortho-hydroxyphenyl)-1,2-diaminoethane (1) and substituted salicylic aldehydes (2). In contrast to symmetric structure of salen ligand (3), salen-Co(III) complexes (4) showed dissymmetric molecular structure due to participation of three hydroxyl groups in complex formation. One of the salens (3b) revealed decrease in Cyclin D1 expression, which represents anti-cancer property.
Production of Bacteriophytochrome Specific Antibodies of Deinococcus radiodurans
Kim, Tae-Lim ; Hahn, Tae-Ryong ; Bhoo, Seong-Hee ;
Journal of Applied Biological Chemistry, volume 53, issue 2, 2010, Pages 112~115
DOI : 10.3839/jabc.2010.021
To analyze the surface properties of bacteriophytochrome (BphP), five (2B8, 2C11, 3B2, 3D2, 3H7) anti-BphP monoclonal antibodies were produced by using full-length of BphP of Deinococcus radiodurnas. 2B8 and 2C11 preferentially recognized the epitopes at N-terminal region of BphP, whereas 3B2, 3D2 and 3H7 showed preferential affinities to the epitopes of C-terminal region of BphP.
Composition Analysis and Antioxidative Activity of Maengjong-juk (Phyllostachys pubescence) Leaves Tea
In, Man-Jin ; Park, Min-Kyung ; Kim, So-Young ; Chae, Hee-Jeong ; Chae, Moon-Whoan ; Sone, Jin ; Ji, Ho-Seon ; Han, Kwang-Sik ; Kim, Dong-Chung ;
Journal of Applied Biological Chemistry, volume 53, issue 2, 2010, Pages 116~119
DOI : 10.3839/jabc.2010.022
A Maengjong-juk (Phyllostachys pubescence) leaves tea was prepared by using a series of drying/roasting and rolling processes. The produced tea contained abundant dietary fibers (60.42%),
-carotenes (7.9 mg RE%), and calcium (437.1 mg%). Also the polyphenol content (
-tea) and DPPH free radical scavenging activity (
) of Maengjong-juk leaves tea were greatly higher than those of simply dried leaves. These results imply that the tea preparation is effective to utilize the useful components of Maengjong-juk leaves.