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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
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Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 53, Issue 4 - Dec 2010
Volume 53, Issue 3 - Sep 2010
Volume 53, Issue 2 - Jun 2010
Volume 53, Issue 1 - Mar 2010
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The Optimization for Functional Expression of Arabidopsis Thaliana AtPIP2-1 in Xenopus laevis Oocyte
Kim, Hyun-Mi ; Hwang, Hyun-Sik ; Lee, Suk-Chan ; Jo, Su-Hyun ; Kim, Beom-Gi ;
Journal of Applied Biological Chemistry, volume 53, issue 4, 2010, Pages 189~194
DOI : 10.3839/jabc.2010.034
We confirmed the hypo-osmotic shock strengths and duration, different type of vectors, and subcelluar localization to identify the optimum analysis condition of plant aquaporin activity in Xenopus ooctye using Arabidopsis thaliana AtPIP2-1 gene. Six minutes and 1/5ND buffer hypoosmotic shock treatment was the best condition to show the maximum swelling of Xenopus oocytes where AtPIP2-1 was expressed using pcDNA3.1 vector. AtPIP2-1 protein was expressed more efficiently in pGEMHE vector which has 5' and 3' UTR (untranslation region) of Xenopus
-GLOBIN gene in multiple cloning site than in pcDNA3.1 vector. Also green fluorescence of GFP fused to AtPIP2-1 was detected onto oocyte plasmamembrane where is the proper subcellular localization target of AtPIP2-1.
Biochemical Characterization of Cysteine(-) Mutant Alanine Racemase from Bacillus pseudomycoides
Kang, Han-Chul ; Yoon, Sang-Hong ; Lee, Chang-Muk ; Koo, Bon-Sung ;
Journal of Applied Biological Chemistry, volume 53, issue 4, 2010, Pages 195~201
DOI : 10.3839/jabc.2010.035
A gene encoding an alanine racemase in B. pseudomycoides was cloned and one (Cys316) or both of two cysteines (Cys316 and Cys365) was (were) substituted with alanine. The cysteine (-) alanine racemases were expressed in E. coli BL21 (DE3) using a pET-21 vector. The expressed enzymes were purified through affinity chromatography using 6xHis ligand. The purified enzymes all showed major one bands by SDS-PAGE analysis, corresponding to 46 kDa. The cysteine (-) alanine racemases as well as the wild type enzyme showed alanine racemase activities, indicating that the enzyme is an alanine racemase and the cysteines in the enzyme may not be involved in the catalysis and/or substrate binding. Thermal stabilities of Cys (-) alanine racemases decreased considerably and half-lives were 26 (wild type), 21 (C316A) and 18 min (C316-365A), respectively at
pH 8.0, suggesting that cysteine is considerably contributive to the thermal stability of the alanine racemase.
Suppressive Effect of Aqueous Extract of Red-Ginseng on the Herbicide-induced DNA Damage and Hemolysis
Seo, Yoo-Na ; Lee, Mi-Young ;
Journal of Applied Biological Chemistry, volume 53, issue 4, 2010, Pages 202~206
DOI : 10.3839/jabc.2010.036
The effects of aqueous extracts of red ginseng on the damage of DNA and erythrocyte by herbicides were evaluated using comet assay and hemolysis assay. Notably, the oxidative DNA damage and erytbrocyte hemolysis by 2,4-D (2,4-dichlorophenoxyacetic acid) and 2,4,5-T (2,4,5-trichlorophenoxyacetic acid) were significantly suppressed by red ginseng treatment. Moreover, red ginseng could suppress significantly paraquat-induced oxidative DNA damage and hemolysis. These suppressive effects of red ginseng on the herbicide-induced damages might be due to the antioxidant components.
Isolation and Identification of Sterol Compounds from the Red Kohlrabi (Brassica oleracea var. gongylodes) Sprouts
Lee, Jae-Woong ; Lee, Dae-Young ; Cho, Jin-Gyeong ; Baek, Nam-In ; Lee, Youn-Hyung ;
Journal of Applied Biological Chemistry, volume 53, issue 4, 2010, Pages 207~211
DOI : 10.3839/jabc.2010.037
The sprouts of Brassica oleracea var. gongylodes were extracted with 100% MeOH and the concentrated extract was partitioned with EtOAc, n-BuOH and
, successively. From the EtOAc fraction, three sterols were isolated through the repeated silica gel and ODS column chromatographies. On the basis of physico-chemical and spectroscopic data including NMR, MS, and IR, the chemical structures of the sterols were determined as
-sitosterol (1), brassicasterol (2), and 7-ketobrassicasterol (3). Compound 1 is usually observed in plant. Compounds 2 is observed in Brassica sp., and compounds 3 have very rarely occurred in natural source including plant.
Antioxidant and Antibacterial Activities, and Tyrosinase and Elastase Inhibitory Effect of Fermented Omija (Schizandra chinensis Baillon.) Beverage
Cho, Eun-Kyung ; Cho, Hea-Eun ; Choi, Young-Ju ;
Journal of Applied Biological Chemistry, volume 53, issue 4, 2010, Pages 212~218
DOI : 10.3839/jabc.2010.038
This study was aimed to investigate the contents of chemical components and the biological activity of fermented Omija (Schizandra chinensis Baillon.) beverage (FOB), which have not been well known up to now. General compositions and the contents of mineral and vitamin of FOB were lower than Omija before fermentation (OBF). In addition, most sucrose was digested into glucose and fructose in FOB. This suggests that the beneficial contents may be made from general compositions by fermentation, though more evidences are needed. Antioxidant activity of FOB was measured by using 2,2-diphenyl-l-picryl-hydrazyl (DPPH) free radical scavenging activity and superoxide dismutase-like activity (SODA). DPPH radical scavenging activity and SODA were increased in a dose-dependent manner, and were about 79.7% and 60.8% at four times diluted FOB (25% of FOB), respectively. The FOB also showed strong antibacterial activity on Escherichia coli. Beauty effect of FOB was demonstrated by the analysis of tyrosinase and elastase inhibitory activities, and they were remarkably increased in a dose-dependent manner. Tyrosinase inhibitory activity was about 82% at two times diluted FOB (50% of FOB) and elastase inhibitory activity was 78.2% at five times diluted FOB (20% of FOB). These results suggest that FOB has a strong antioxidant activity, and tyrosinase and elastase inhibitory effects.
Effect of Medicinal Herbs Extracts on Physicochemical Characteristics of Cooked Rice
Cho, Yong-Sik ; Lee, Kyung-Ha ; Ha, Hyun-Jee ; Park, Shin-Young ; Choi, Yoon-Hee ; Kim, Eun-Mi ;
Journal of Applied Biological Chemistry, volume 53, issue 4, 2010, Pages 219~224
DOI : 10.3839/jabc.2010.039
This study was conducted to investigate the effect of medical herbs extracts on physicochemical characteristics of cooked rice. Three types (CLP 1, CLP 2, CLP 3) of extracts were prepared using 11 medicinal herbs, respectively. Polished rice was cooked with different concentration of medicinal herbs extracts by electric cooker. The moisture contents in cooked rice were decreased by increased concentration of herbs extracts, while total polyphenol contents were significantly increased. DPPH radical scavenging activities of cooked rice obviously were increased as herb extracts level increased. The consistency and setback value of the rice flour was reduced at all of the extracts, while breakdown and initial pasting temperature was increased. Color of cooked rice was shown different comparing to the control. However, the hardness, adhesiveness, springiness, cohesiveness and gumminess of cooked rice was increased depending on the concentration of extracts. These results showed that medicinal herbs extracts, which can benefit human health, is inducing the changes of physicochemical characteristics of cooked rice.
3D-QSARs analyses for Tyrosinase Inhibitory Activity of 2-Phenyl-1,4-benzopyrone (Flavones) Analogues and Molecular Docking
Park, Joon-Ho ; Sung, Nack-Do ;
Journal of Applied Biological Chemistry, volume 53, issue 4, 2010, Pages 225~231
DOI : 10.3839/jabc.2010.040
To understand the inhibitory activity with changing hydroxyl substituents (
) of polyhydroxy substituted 2-phenyl-l,4-benzopyrone analogues (1-25) against tyrosinase (PDB ID: oxy-form; 1WX2), molecular docking and the three dimensional quantitative structure-activity relationships (3D-QSARs: Comparative molecular field analysis (CoMFA) & Comparative molecular similarity indices analysis (CoMSIA)) were studied quantitatively. The statistically best models were CoMFA 1 and CoMSIA 1 model from the results. The optimized CoMSIA 1 model with the sensitivity of the perturbation and the prediction produced (
=0.51l) by a progressive scrambling analysis were not dependent on chance correlation. The inhibitory activities with optimized CoMSIA 1 model were dependent upon electrostatic factor (51.4%) of substrate molecules. Contour mapping the 3D-QSAR models to the active site of tyrosinase provides new insight into the interaction between tyrosinase as receptor and 2-phenyl-l,4-benzopyrone analogues as inhibitor. Therefore, the results will he able to apply to the optimization of a new potent tyrosinase inhibitors.
Treatment of Pollutants in Free Water Surface Constructed Wetlands with Lotus (Nelumbo nucifera) Cultivation Pond
Han, Myung-Ja ; Seo, Dong-Cheol ; Kang, Se-Won ; Lee, Yong-Chol ; Bang, Seok-Bae ; Chae, Jung-Heon ; Kim, Kap-Soon ; Park, Jong-Hwan ; Chang, Nam-Ik ; Heo, Jong-Soo ; Cho, Ju-Sik ;
Journal of Applied Biological Chemistry, volume 53, issue 4, 2010, Pages 232~238
DOI : 10.3839/jabc.2010.041
In order to investigate the treatment efficiency of pollutants in free water surface constructed wetlands (FWS CWs) with lotus (Nelumbo nucifera) cultivation pond, the experiment was consisted of two sites (site I and II) in Lake Juam, Korea. The sites were configured a lotus cultivation pond (with fertilizer application) - a dropwort bed - a reed bed for site I, and a lotus cultivation pond (without fertilizer application) - a dropwort bed - a reed bed for site II. Removal rate of COD in site I and II were 13.3% and 26.0%, respectively. Removal rate of total nitrogen (TN) was 29.7% for site I, and 36.3% for site II. Removal rate of total phosphorus (TP) in site I and II were 36.0% and 36.5%, respectively. COD, TN and TP in effluent from site I (with fertilizer) was higher than that in site II (without fertilizer), showing that COD, TN and TP in effluent were strongly influenced by fertilizer addition. Therefore, in order to satisfy established water-quality standards, the amount of fertilizer used in lotus cultivation showed be evaluated.
The Antibiotic Effect of Acetic acid on Helicobacter pylori
Kim, Sun-Young ; Kwon, Woo-Je ; Kang, Sang-Mo ;
Journal of Applied Biological Chemistry, volume 53, issue 4, 2010, Pages 239~247
DOI : 10.3839/jabc.2010.042
Helicobacter pylori (H. pylori) is the main causal bacteria occurring stomach diseases such as gastritis and gastric ulcer. These bacteria are found in most adults' stomach. Especially, 60~90% of H. pylori is found in Korean stomach. As a lot of curing methods have been applied to remove H. pylori and certain effects have been done but it's impossible to remove it perfectly with the existing medicines for curing. Therefore, it's very urgent to develop a certain substance showing more excellent effect than the existing medicines. In this study, it wasfound that organic acids can be accessed easily, inserted into mouth for curing and has excellent sterilizing effect among the substances showing excellent antibiotic power. Among them, acetic acid showed the most excellent effect. To confirm the refraining power against H. pylori, we performed tests through in vitro contact testing methods by concentration and tsta. In the result, H. pylori were terminated perfectly in the solution of acetic acid more than 0.3% within 1 minute. With a base of the result of In vitro test, It was performed in vivo test. As the results, H. pyloriwere terminated perfectly on 0.2% solution of acetic acid from the result of confirming through urease test, ELISA method and RT-PCR. Therefore, the result of this research will be very useful information in developing the functional foodstuffs using acetic acid in order to terminate H. pylori on the people's stomach, who suffers from H. pylori.
Development of Phototactic Test Apparatus Equipped with Light Source for Monitoring Pests
Oh, Min-Seok ; Lee, Hoi-Seon ;
Journal of Applied Biological Chemistry, volume 53, issue 4, 2010, Pages 248~252
DOI : 10.3839/jabc.2010.043
This study presented a development of a phototactic apparatus for pest monitoring. We carried out behavioral experiment against various pest species by using the phototactic chamber. Consequently, the phototactic apparatus was confirmed suitable result of behavioral experiment. Therefore, we believed that the test apparatus help to understand the phototactic responses of various pests.