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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Applied Biological Chemistry
Journal Basic Information
Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 54, Issue 4 - Dec 2011
Volume 54, Issue 3 - Sep 2011
Volume 54, Issue 2 - Jun 2011
Volume 54, Issue 1 - Mar 2011
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The Physical Properties of -uffed Snacks (ppeongtuigi) Added with Sweet Potato Flours
Cheon, Seon-Hwa ; Eun, Jong-Bang ;
Journal of Applied Biological Chemistry, volume 54, issue 3, 2011, Pages 147~152
DOI : 10.3839/jabc.2011.025
The physical properties of puffed snack by pellet with sweet potato flour, brown rice flour and wheat flour were evaluated at different moisture content, puffing temperature, and puffing time. The sweet potato pellets were tempered to 14, 16, and 18% moisture content and were puffed at 233, 238, and
for 4, 5 and 6 s. The whitness (
) value decreased as the heating temperature and time increased. The redness (
) and yellowness (
) values increased with increasing heating temperature and time. The specific volume of sweet potato puffed snacks showed an increasing trend with higher puffing conditions. The hardness of sweet potato puffed snacks increased as heating time and heating temperature increased. The sweet potato puffed snacks prepared with increasing moisture content (18%), heating times (6 s), and heating temperatures (
) were awarded the highest scores for most of the sensory attributes and hence declared as best sweet potato puffed snacks.
Characteristics of Culture Conditions for the Production of Crude Biosurfactant by Bacillus subtilis JK-1
Kim, Ji-Yeon ;
Journal of Applied Biological Chemistry, volume 54, issue 3, 2011, Pages 153~158
DOI : 10.3839/jabc.2011.026
Optimal culture conditions were characterized for production of crude biosurfactant of Bacillus subtilis JK-1. During incubation of B. subtilis JK-1, the bacterial growth pattern, changes of the surface tension at variable temperatures, pH and NaCl concentrations in bacterial culture medium were studied. The strain was able to grow and produce biosurfactant at
, in the pH range of 6-10, and at 0-10% (w/v) NaCl. In case, culture broth pH was gradually changed to neutral or weak alkaline. Optimal culture conditions for crude biosurfactant production were at
and pH 7.0 after 48 h incubation and the surface tension of biosurfactant was 24.0 mN/m. Besides, as the concentration of NaCl was increased from 0 to 10% (w/v), the growth was decreased, pH of the culture broth was converted from weak alkaline to acidic, and the surface tension rised.
Isolation and Identification of Antimicrobial Compounds against Helicobacter pylori from Rosemary (Rosmarinus officinalis L.) Extracts
Yoon, So-Jung ; Kim, Jin-Sung ; Jo, Bun-Sung ; Kim, Jeung-Hoan ; Lee, Sun-Ho ; Ahn, Bong-Jeun ; Cho, Young-Je ;
Journal of Applied Biological Chemistry, volume 54, issue 3, 2011, Pages 159~165
DOI : 10.3839/jabc.2011.027
Rosmarinus officinalis extracts had a significant antimicrobial activity against Helicobacter pyori. Total phenolic contents and inhibition zone of rosemary extracts were estimated to be 25.7 mg/g and 14 mm at
of phenolic contents, respectively. The Sephadex LH-20 and MCI-gel CHP-20 column chromatographic separations for the phenolic extracts from R. officinalis leaves led to isolation of five acids, whose structures were determined as protocatechuic acid (A), coumaric acid (B), caffeic acid (C), chlorogenic acid (D), and rosmarinic acid (E), from interpretation of spectroscopic data including nagative fast atom bombardment (FAB)-mass,
-NMR, and IR. All isolated compounds were tested for antimicrobial activity against H. pyori. The purified single compound showed less antimicrobial activity against H. pylori than the mixed purified compounds, which generate A+B, A+E, C+D, C+E (each
) excellent as large clear zone by synergy effect. These results indicate rosemary extracts are preventive agents against H. pyori.
The Promotion of Cell Attachment and Proliferation on Silk Fibroin
Jo, You-Young ; Kweon, Hae-Yong ; Lee, Kwang-Gill ; Nam, Sung-Hee ; Lee, Heui-Sam ; Yeo, Joo-Hong ;
Journal of Applied Biological Chemistry, volume 54, issue 3, 2011, Pages 166~170
DOI : 10.3839/jabc.2011.028
Silk fibroin, a natural protein produced by silkworm, is a good biomaterial which has biodegradability and biocompatibility. To ascertain the effects of silk fibroin on cell growth, silk fibroin films were prepared using silk fibroin aqueous solutions of various concentrations. We investigated the attachment, proliferation, morphology of the cells and the expression levels of genes related to cell attachment and growth on the silk fibroin films. When the cells were cultured on the 0.1 and 1% silk fibroin film, the cell adhesion ability was very excellent. Particularly, overall cell growth on the 1% silk fibroin film was definitely superior to the others. Also, expression levels of genes related cell growth were increased on the 0.1 and 1% silk fibroin film. These results suggest silk as a material for medical applications.
Antioxidant Activities and Nitric Oxide Production of Medicine Plants in Gyeongsangbukdo (Carthamus tinctorius seed, Cyperus rotundus, Schizonepeta tenuifolia, Polygonatum odoratum var. pluriflorum, Paeonia lactiflora)
Hwang, Eun-Young ; Kim, Dong-Hee ; Kim, Hui-Jeong ; Hwang, Jo-Young ; Park, Tae-Soon ; Lee, In-Sun ; Son, Jun-Ho ;
Journal of Applied Biological Chemistry, volume 54, issue 3, 2011, Pages 171~177
DOI : 10.3839/jabc.2011.029
This study was carried out to search for natural anti-oxidants and anti-inflammatory compounds from 5 medicinal plants (Carthamus tinctorius seed, Cyperus rotundus, Schizonepeta tenuifolia, Polygonatum odoratum var. pluriflorum, and Paeonia lactiflora). These plants were extracted with 70% ethanol. In order to measure total antioxidant activity of flavonoids, polyphenol content was measured. Radical scavenging activities of extracts were examined using a-a-Diphenyl-
), 2,2-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid (
), ferric reducing antioxidant power (FRAP) and superoxide anion radical assays. C. tinctorius seed extracts showed the highest polyphenol and flavonoid contents as well as strong
, FRAP, and superoxide anion radical scavenging activity. Also, C. tinctorius seed extracts showed the highest nitric oxide (NO) production inhibitory effect. Theses results indicate that the C. tinctorius seed extracts can be used as a functional material due to their effective anti-oxidative and antiinflammatory activities.
Isolation of Flavonoids and Lignans from the Stem Wood of Lindera obtusiloba Blume
Seo, Kyeong-Hwa ; Baek, Mi-Young ; Lee, Dae-Young ; Cho, Jin-Gyeong ; Kang, Hee-Cheol ; Ahn, Eun-Mi ; Baek, Nam-In ; Lee, Youn-Hyung ;
Journal of Applied Biological Chemistry, volume 54, issue 3, 2011, Pages 178~183
DOI : 10.3839/jabc.2011.030
The stem woods of Lindera obtusiloba Blume were extracted in 80% aqueous methanol and the concentrated extract was partitioned with ethyl acetate (EtOAc), butanol (n-BuOH), and
, successively. From the EtOAc and n-BuOH fractions, five compounds were isolated through the repeated silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatographies. On the basis of spectroscopic data including mass spectrometry, IR,
-NMR, distortionless enhancement by polarization transfer, and two-dimensional-NMR gradient correlated spectroscopy (gCOSY), gradient heteronuclear single quantum correlation (gHSQC), gradient heteronuclear multiple bonding connectivity (gHMBC), the chemical structures of the compounds were determined as asarinin (1), (+)-catechin (2), (-)-epicatechin (3), hyperin (4), and nudiposide (5). Compounds 1 and 5 were isolated for the first time from the stem wood of L. obtusiloba Blume.
Antioxidative and Protective Activity of Polysaccharide Extract from Artemisia iwayomogi Kitamura Stems on UVB-Damaged Mouse Epidermis
Ahn, Byung-Yong ; Jung, Mun-Yhung ;
Journal of Applied Biological Chemistry, volume 54, issue 3, 2011, Pages 184~189
DOI : 10.3839/jabc.2011.031
Polysaccharide (PS) was fractionated from hot-water extract of Artemisia iwayomogi Kitamura stems. PS showed considerably higher hydroxyl radical scavenging activity than caffeic acid and glutathione. PS showed lower superoxide anion radical scavenging activity than hydroquinone and ascorbic acid. The scavenging activity of PS on the reactive oxygen species (ROS) induced by human neutrophils with zymosan was determined by the lucigenin-enhanced chemiluminescence assay. The scavenging effect of the PS on ROS as determined by the chemiluminescence assay was about 2-fold stronger than that of ascorbic acid at the same concentration. PS significantly decreased protein carbonyl and malonaldehyde contents in UVB irradiated skin homogenates, which was comparable to glutathione at the same concentration. This result suggested that PS derived from A. iwayomogi Kitamura stems may be a potent candidate as functional compound for the protection on UVB induced skin damage in cosmetics.
Effect of Edible Coatings Containing Soy Protein Isolate (SPI) on the Browning and Moisture Content of Cut Fruit and Vegetables
Shon, Jin-Han ; Choi, Yong-Hwa ;
Journal of Applied Biological Chemistry, volume 54, issue 3, 2011, Pages 190~196
DOI : 10.3839/jabc.2011.032
Effectiveness of edible coatings containing soy protein isolate (SPI), in reducing oxidative browning and moisture loss during storage (
) of cut apples, potatoes, carrots, and onions was investigated. The SPI coatings were shown to have antioxidative activity. Furthermore, addition of carboxymethyl cellulose (CMC) to the formulations significantly improved its antioxidative activity. Oxidative discoloration, as determined by Commission Internationale De I'Eclairage (CIE) lightness (
), redness (
), and yellowness (
) color scale, was significantly reduced (p <0.05) by SPI coating treatments over a storage time of 120 min. Loss of lightness was reduced by SPI coatings with and without CMC. These respectively showed 4.03 and 3.71% change of
value compared to 8.56% for control. Browning of the control in cut potatoes was significantly increased by 106.6% in contrast to 34.3 and 35.2% for SPI coatings with and without CMC, respectively. The
values also reflected effectiveness of SPI. Moisture barrier effect was significantly better for the treatments, compared to the control. SPI coatings reduced moisture loss in apples and potatoes, respectively, by 21.3 and 29.6% over the control. Cut onions did not show any treatment effect both in terms of browning and moisture loss. SPI coatings prove to be good moisture barrier and antioxidative property.
Anti-obesity Effect of Monascus pilosus Mycelial Extract in High Fat Diet-induced Obese Rats
Lee, Sang-Il ; Kim, Jae-Won ; Lee, Ye-Kyung ; Yang, Seung-Hwan ; Lee, In-Ae ; Suh, Joo-Won ; Kim, Soon-Dong ;
Journal of Applied Biological Chemistry, volume 54, issue 3, 2011, Pages 197~205
DOI : 10.3839/jabc.2011.033
This study was carried out to investigate the dietary effects of Monascus pilosus mycelial extract on obesity in high-fat with cholesterol-induced obese rat models. It was observed that M. pilosus mycelial extract contains
of total monacolin K without citrinin by highperformance liquid chromatography (HPLC). The rats were randomly divided into 2 groups; normal control and a high-fat with cholesterol diet group. The high-fat with cholesterol diet group was fed a 5L79 diet with an added 15% lard and 1% cholesterol supplemented diet for 3 weeks for induction of obesity. After induction, obesity was confirmed by checking obesity indexes, the animals were divided into 4 groups (n=5); first, the normal control (NC), and then taken from the obese model of rats, a high-fat with cholesterol diet obesity control group (HF), 0.5% M. pilosus mycelial extract supplemented high-fat with cholesterol diet group (MPMs), 2% conjugated linoleic acid supplemented high-fat with cholesterol diet group (CLA) for 7 weeks. Body weight gains, obesity indexes, and body fat contents in the experimental groups (MPMs and CLA) were decreased compared with HF group. Feed Efficiency Ratio (FER) in MPMs was significantly lower than that of HF without change of feed intake. These results suggested that the anti-obesity effects of the M. pilosus mycelial extracts (MPMs) could prevent obesity induced by high-fat with cholesterol diet possibly via inhibition of lipid absorption.
Protective Effect of Monascus pilosus Mycelial Extract on Hepatic Damage in High-Fat Diet Induced-obese Rats
Lee, Sang-Il ; Kim, Jae-Won ; Lee, Ye-Kyung ; Yang, Seung-Hwan ; Lee, In-Ae ; Suh, Joo-Won ; Kim, Soon-Dong ;
Journal of Applied Biological Chemistry, volume 54, issue 3, 2011, Pages 206~213
DOI : 10.3839/jabc.2011.034
Hepatoprotective effects of Monascus pilosus mycelial ethanol extract (MPME) were examined in high-fat diet induced-obese rats. The rats were randomly divided into 2 groups; normal control (NC) and a high-fat and high cholesterol diet group (HFC). The HFC diet group was fed a 5L79 diet supplemented with 15% lard and 1% cholesterol for 3 weeks for induction of obesity. And then, the rats were divided into 4 groups (n=5); the NC, a HFC diet obesity control group (HF), 0.5% MPME supplemented HFC diet group (MPM), and 2% conjugated linoleic acid (CLA) supplemented HFC diet group for 7 weeks. Whereas the daily weight gain of NC and HFC groups were 3.48 g and 4.48 g, respectively, those of MPM and CLA were 3.09 g and 4.38 g, respectively. Furthermore, activity of serum alanine and aspartic aminotransferase in HF was markedly higher than those of NC group, but, the activity in MPM and CLA was significantly lower than HF. Hepatic reduced glutathione content in MPM and CLA was higher than HF. On the contrary, hepatic lipid peroxide content in MPM and CLA was significantly lower than HF. In conclusion, although the precise mechanisms of the hepatoprotective effects of the MPME in this study are unknown, our study provides experimental evidence that MPME may prevent obesity and hepatic damage by high-fat and high cholesterol diet via inhibition of lipid absorption and induction of reactive oxygen spices scavenging enzyme such as superoxide dismutase.
Residual Characteristics of Methyl Bromide and Hydrogen Cyanide in Banana, Orange, and Pineapple
Park, Min-Goo ; Sung, Bo-Kyung ; Cho, Jae-Young ;
Journal of Applied Biological Chemistry, volume 54, issue 3, 2011, Pages 214~217
DOI : 10.3839/jabc.2011.035
Fumigant, methyl bromide which is used in most countries for plant quarantine, has been designated and under control as ozone depleting substance. For developing alternative chemicals for methyl bromide, many countries have been intensifying their capacity. This study was carried out to investigate the residue patterns of hydrogen cyanide which is commonly used for plant quarantine. Hydrogen cyanide was treated onto the orange, banana, and pineapple at recommended and double doses and then sampling was done at 1 and 3 day after fumigation treatment. Residue of hydrogen cyanide was found safe because average residue levels on orange, banana, and pineapple after 3 days of fumigation treatment were
ppm, which were lower than the MRLs of Korea (5 ppm), Japan (5 ppm), USA (50 ppm), and Canada (25 ppm). Hydrogen cyanide are expected to be used as alternative chemicals for methyl bromide fumigant for orange, banana, and pineapple.
Water Quality of Agricultural Groundwater in Western Coast Area and Eastern Mountain Area of Jeollabuk-do
Jo, Jae-Yeong ;
Journal of Applied Biological Chemistry, volume 54, issue 3, 2011, Pages 218~224
DOI : 10.3839/jabc.2011.036
This study was conducted to estimate the water quality of agricultural groundwater well located in Buan-gun, Jinan-gun, Sunchang-gun, and Jangsu-gun of Jeollabuk-do. The groundwater samples were collected at 328 sites (Buan-gun: 158, Jansu-gun: 45, Sunchang-gun: 32, Jinan-gun: 93, respectively). We measured 4 kinds of general contaminants (pH,
, and COD) and 10 kinds of specific contaminants (Cd, As,
, Hg, phenol, Pb,
, organophosphorus, trichloroethylene, and tetrachloroethylene). Generally, the level of general contaminants and specific contaminants in the agricultural groundwater was suitable for water quality standard in all sites for agricultural irrigation water. Exceptionally, chloride concentrations were exceeded water quality standard of agricultural groundwater at some sites in western coast area of Jeollabuk-do. Although water quality standards in agricultural groundwater have been suitable, the water contaminants of agricultural groundwater in western coast area were gradually increased than eastern mountain area.
Relationship between Concentration of Phosphorus, Turbidity, and pH in Water and Soil under Aerobic and Anaerobic Conditions
Min, Young-Hong ; Kang, Sam-Woo ; Lee, Hoi-Seon ; Chung, Nam-Hyun ;
Journal of Applied Biological Chemistry, volume 54, issue 3, 2011, Pages 225~229
DOI : 10.3839/jabc.2011.037
This research was carried out to elucidate the impacts of dissolved oxygen (DO) concentration to phosphorus release mechanism from soil into water in lakes.
contributed to the growth of the total phosphorus (T-P).
was steadily increasing because water was accumulating
. T-P was closely related to turbidity, pH, and DO. We found that DO had decreased because DO was consumed in organic matter decomposition, and that the resulting anaerobic decomposition occurred whenever water had run out of DO. We also found that pH had decreased sharply by production of organic acid by the anaerobic decomposition and that T-P decreased because a decrease in pH removed turbidity by precipitation. T-P was dissolved without microbial decomposition. This mechanism was of great importance in lakes because phosphorus is released from soil into water.