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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Applied Biological Chemistry
Journal Basic Information
Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 56, Issue 4 - Dec 2013
Volume 56, Issue 3 - Sep 2013
Volume 56, Issue 2 - Jun 2013
Volume 56, Issue 1 - Mar 2013
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Protective Effect of Rhodiola sachalinensis A. Bor on Excessive Exercise Stress
Lee, Yeonmi ; Hong, Hee-Do ; Choi, Sang Yoon ;
Journal of Applied Biological Chemistry, volume 56, issue 1, 2013, Pages 1~3
DOI : 10.3839/jabc.2013.001
Rhodiola sachalinensis A. Bor is a plant naturally grows in a high mountain areas. Aim of this study was to evaluate the effects of R.sachalinensis A. Bor extract against excessive exercise induced stress. R.sachalinensis A. Bor extract significantly inhibited L6 muscle cell death and ATP reduction caused by
damage. In addition, the oral administration of R. sachalinensis A. Bor extract in mice improved the 43.9% of treadmill running time and blood profiles of glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, lactate dehydrogenase, creatinine, lactate when compared to distilled water. These results suggested that R. sachalinensis A. Bor can attenuate excessive exercise induced damage.
Comparison of the Nutritional Compositions of Insect-Resistant and Glufosinate-Tolerant Rice and Conventional Rice
Park, Soo-Yun ; Lee, Si Myung ; Yeo, Yunsoo ; Kweon, Soon Jong ; Cho, Hyun Suk ; Kim, Jae Kwang ;
Journal of Applied Biological Chemistry, volume 56, issue 1, 2013, Pages 5~9
DOI : 10.3839/jabc.2013.002
The nutritional composition of transgenic rice (Agb0101) with bar and modified cry1Ac1 genes grown with herbicides was compared with that of its non-transgenic counterpart. The analyzed components (proximates, amino acids, fatty acids, minerals, vitamins, trypsin inhibitors, and phytic acid) in the herbicide-treated Agb0101 brown rice were substantially equivalent to those of its non-transgenic counterpart.
Effect of Anti-Microbial Materials on Storages of Ssamjang
Kang, Bo-Ra ; Im, Go-Eun ; Kim, Dong-Han ;
Journal of Applied Biological Chemistry, volume 56, issue 1, 2013, Pages 11~17
DOI : 10.3839/jabc.2013.003
The effect of additives on the quality of ssamjang was investigated during storage. The L-, a- and b-values of ssamjang decreased gradually during storage, and the total color difference (
) increased in the control group. The gas production of ssamjang was reduced in K-sorbate, alcohol and mustard added groups. The number of yeast increased rapidly up to 6 weeks of storage, then decreased in the mustard, alcohol, and K-sorbate added groups. The oxidation-reduction potential and water activity decreased until 6 weeks and 12 weeks, respectively, and then increased gradually. Titratable acidity increased with concomitant decrease in pH, however, it increased slightly in the K-sorbate and ethanol added groups. Reducing sugar content increased until 9 weeks of storage, except the turmeric added group. Alcohol content increased until 15 weeks in the Japanese apricot juice, and turmeric added group. The sensory test result for the taste, flavor and overall acceptability showed that mustard added ssamjang was more acceptable than other groups.
Effects of Dehydrating Agents on the Physicochemical Properties of Dried Plum (Prunus salicina L.) Slices
Kim, Min-Sung ; Kang, Ji-Hoon ; Chung, Kyung-Sook ; Won, Misun ; Song, Kyung Bin ;
Journal of Applied Biological Chemistry, volume 56, issue 1, 2013, Pages 19~22
DOI : 10.3839/jabc.2013.004
Plum (Prunus salicina L.) slices were dehydrated with red algae extract (RAE) at a concentration of 30% (w/w), and the dried samples were compared with maltodextrin (MD)-treated and hot-air dried samples in terms of physicochemical properties such as rehydration ratio, ascorbic acid, microstructure, and color. The rehydration ratios and colors of RAE-treated plum slices were better than those of MD-treated and hot-air dried samples. The ascorbic acid contents of RAE-treated samples were higher and their microstructures were finer than those of MD-treated or hot-air dried samples. These results suggest that plum slices can be dehydrated with RAE without loss of quality.
Isolation and Identification of Flavonoids from the Roots of Brassica rapa ssp.
Jeong, Rak-Hun ; Wu, Qian ; Cho, Jin-Gyeong ; Lee, Dae-Young ; Shrestha, Sabina ; Lee, Min-Ho ; Lee, Kyung-Tae ; Choi, Myung-Sook ; Jeong, Tae-Sook ; Ahn, Eun-Mi ; Chung, Hae-Gon ; Rho, Yeong-Deok ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 56, issue 1, 2013, Pages 23~27
DOI : 10.3839/jabc.2013.005
The roots of Brassica rapa ssp. were extracted with 95% aqueous ethanol and the concentrated extracts were partitioned using ethyl acetate (EtOAc), n-butyl alcohol and
, successively. From the EtOAc fraction, five flavonoids were isolated through repeated silica gel and octadecyl silica gel (ODS) column chromatography (c.c.). Based on NMR, mass spectrometry (MS) and IR spectroscopic data, the chemical structures of the compounds were determined to be licochalcone A (1), 4,4'-dihydroxy-3'-methoxychalcone (2), liquirtigenin (3), liquiritin (4), and isoliquiritin (5). This is the first report of these compounds isolated from the root of this plant.
Evaluation of Residual Pesticides in Fresh Ginseng Collected in Seoul
Kim, Tae-Rang ; Park, Ki-Hwan ; Jang, Mi-Ra ; Choi, Young-Hee ; Kim, Eun-Hee ; Choi, Chae-Man ; Park, Sung-Kyu ; Yu, In-Sil ; Hwang, In-Sook ; Han, Ki-Young ; Kim, Moo-Sang ; Kim, Jung-Hun ; Chae, Young-Zoo ;
Journal of Applied Biological Chemistry, volume 56, issue 1, 2013, Pages 29~35
DOI : 10.3839/jabc.2013.006
This study was performed to analyze 48 kinds of pesticide residues using gas chromatography (GC)/nitrogen phosphorous detector, GC/micro electron capture detector, GC/mass selective detector, and high performance liquid chromatograph/diode array detector in 186 fresh ginseng samples collected in the Seoul area from 2010 to 2011. Fresh ginseng dietary intakes were estimated using the data from the 2009 Korea National Health and Nutrition examination survey. Residual pesticides were detected in 79 samples (42.5%) with eight different fungicides. Only 20 samples (10.8%) exceeded the maximum residue limits (MRLs) for pesticides registered by the Korea Food & Drug Administration. Among them, tolclofos-methyl residues (10.2%) exceeded the MRL for fresh ginseng in 18 ginseng seedlings and one of the two-year old fresh ginseng plants, and the residual level in just one ginseng seedling violated the MRL for pyrimethanil. The results showed that residual pesticides levels in marketable fresh ginseng around Seoul were relatively safe. The percent acceptable daily intake (%ADI) was calculated using pesticide residues in fresh ginseng and dietary intakes of fresh ginseng. The risk caused by pesticide residues in fresh ginseng was very low.
DNA Repair of Eukaryotes Associated with Non-coding Small RNAs
Kang, Han-Chul ; Yoon, Sang-Hong ; Lee, Chang-Muk ; Roh, Kyung Hee ;
Journal of Applied Biological Chemistry, volume 56, issue 1, 2013, Pages 37~42
DOI : 10.3839/jabc.2013.007
In eukaryotes, most of the genome are transcribed, however only a small proportion of total transcripts encodes for protein, thus resulting in many of noncoding RNAs. In order to recover DNA damage including DNA double-strand breaks (DSBs) eukaryotes have evolved complex mechanisms and these are processed through coordinated mechanisms of protein sensors, transducers, and effectors including RNAs. During recent years, small RNAs have been increasingly studied and gradually considered as key regulators in various aspects of biology. Upon DNA damage, small RNAs including diRNAs (DSB induced RNA) are generated in both plant and human cell lines. Inhibition of their biogenesis has severe influence on DSB repair system.
Analysis of Carotenoids in 25 Indigenous Korean Coral Extracts
Kim, Sang Min ; Kang, Suk-Woo ; Lee, Eun A ; Seo, Eun-Kyoung ; Song, Jun-Im ; Pan, Cheol-Ho ;
Journal of Applied Biological Chemistry, volume 56, issue 1, 2013, Pages 43~48
DOI : 10.3839/jabc.2013.008
In this study, methanol extracts from 25 indigenous Korean corals were prepared and their carotenoid constituents were analyzed by high-performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (HPLC-APCI-MS). Among them, extracts from nine species showed detectable peaks in the HPLC chromatogram at 450 nm and the ultraviolet/visible spectra exhibiting carotenoid-specific characteristics were chosen. The mass data of carotenoid peaks revealed that only peridinin could be identified based on literature comparison and suggested the potential presence of novel carotenoid structures. This is the first reported investigation of indigenous Korean coral carotenoids and further work is needed to explore the carotenoids and their potential roles in the ecosystem of indigenous Korean corals.
Anticancer and Antimicrobial Activities of 13(E)-labd-13-ene-8α,15-diol from Brachyglottis monroi
Kim, Jong-Im ; Choi, Hwa-Jung ; Lee, Jae-Sook ;
Journal of Applied Biological Chemistry, volume 56, issue 1, 2013, Pages 49~51
DOI : 10.3839/jabc.2013.009
In a previous study, we reported that 13(E)-labd-13-ene-
,15-diol (13E) possesses antiviral and anticancer activities. In this study, the anticancer and antimicrobial activities of 13(E) were evaluated against 4 cancer cell lines and 6 bacteria. 13(E) showed inhibitory effect on a variety of cancer cell lines. The
values was 8.3-21.3
. 13(E) was the most effective growth inhibitor of murine leukaemia cell lines P388, producing approximately 8.3
in the cytopathic effect (CPE) method. 13(E) also inhibited the growth of the gram-positive bacteria (Staphylococcus aureus, Bacillus cereus and Listeria monocytogenes) and gram-negative bacteria (Vibrio parahaemolyticus, Escherichia coli and Salmonella enteritidis) with a range of minimum inhibitory concentration (MIC) values from 0.092 to 0.598 mg/mL and gram-negative bacteria were more sensitive to the compound (MIC, 0.092 mg/mL).
Comparison of Liquid Chromatography-Mass/Mass Spectrometry (MS) and Gas Chromatography-MS for Quantitative Analysis of Indole-3-acetic acid and Indole-3-butyric acid from the Concentrated Liquid Fertilizer
Kim, Jin Hyo ; Park, Jong Min ; Choi, Geun-Hyoung ; Park, Yun-Ki ; Im, Geon-Jae ; Kim, Doo-Ho ; Kwon, Oh-Kyung ;
Journal of Applied Biological Chemistry, volume 56, issue 1, 2013, Pages 53~57
DOI : 10.3839/jabc.2013.010
In here, we investigated the quantitative analysis method of indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) with liquid chromatography-mass/mass spectrometry (LC-MS/MS) or gas chromatography-MS. Two ways of clean-up process were investigated for LC-MS/MS instrumental analysis of IAA, but both a simple dilution and hydrophile-lipophile balance (HLB) solid phase extraction (SPE) were not met the optimal recovery rates for quantitative analysis. On the other hand, the clean-up method for GC-MS was finally optimized through HLB-SPE from 250-folds diluted sample and methylation with trimethylsilyl chloride in methanol for 4 h. The limit of detection for methyl ester of IAA and IBA were both 1.4 mg/L, and recovery rates showed 93-107% from the concentrated liquid fertilizer.