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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
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Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 56, Issue 4 - Dec 2013
Volume 56, Issue 3 - Sep 2013
Volume 56, Issue 2 - Jun 2013
Volume 56, Issue 1 - Mar 2013
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Evaluation of Anti-inflammatory Activities and Mechanisms of Microalga Phaeodactylum tricornutum
Kim, Jeong Hwa ; Kim, Sang Min ; Pan, Cheol-Ho ; Choi, Joong-Kook ; Lee, Jae Kwon ;
Journal of Applied Biological Chemistry, volume 56, issue 2, 2013, Pages 61~67
DOI : 10.3839/jabc.2013.011
Due to their diversity and abundancy, marine resources have emerged as important biological resources to compensate the limited sources of terrestrial biological materials. Phaeodactylum tricornutum (PT) is one of classical model diatoms most widely studied for its ecology, physiology, biochemistry and molecular biology. In this study, four different PT extracts on lipopolysaccharide (LPS)-stimulated macrophages were compared for anti-inflammatory effect and investigated for the underlying mechanisms. The extracts of PT inhibited nitric oxide production from LPS stimulated RAW 264.7 cells in a dose dependent manner. These extracts also inhibited the expression of mRNA and production of proteins of pro-inflammatory cytokines such as interleukin (IL)-
, IL-6 and tumor necrosis factor-
. These inhibitory effects were found to be caused by blockage of nuclear factor-
activation and phosphorylation of p38 mitogen-activated protein kinases, extracellular signal-regulated kinases 1 and 2 and c-Jun N-terminal kinase.
Assessing Adjuvants and Extractants Applicable to Environment-friendly Organic Agro-materials
Lee, Woo-Mi ; Yoon, Sung-Ji ; An, Youn-Joo ;
Journal of Applied Biological Chemistry, volume 56, issue 2, 2013, Pages 69~78
DOI : 10.3839/jabc.2013.012
This study reviewed the adjuvants or extractants used in environment-friendly organic agro-materials used in a range of advanced countries or institutes. We observed that potassium hydroxide and fermented ethyl alcohol are generally acceptable extractants, and the inert ingredient list 4 of United States Environmental Protection Agency (US EPA) is applicable for crop production adjuvants.
Storage-life Extension of Maengjong-Juk (Phyllostachys pubescens) Sap Using Heat Treatments
Cho, Eunhye ; Kim, Soyoung ; Chae, Hee Jeong ; Kim, Dong Chung ; In, Man-Jin ;
Journal of Applied Biological Chemistry, volume 56, issue 2, 2013, Pages 79~81
DOI : 10.3839/jabc.2013.013
In order to increase the shelf-life of Phyllostachys pubescens sap heating conditions were investigated. To evaluate the quality changes of Phyllostachys pubescens sap before and after heating at
for 30 min, pH, total microbial number and sensory characteristics were measured during storage periods at
. During 10 days, the quality and sensory characteristics of Phyllostachys pubescens sap were maintained.
Bio-active Substances and Physiological Activity of Pears
Min, Tae Sun ; Park, Min Jung ; Moon, Jae Hak ; Kim, Wol Soo ; Lee, Sang Hyun ; Cho, Young Don ; Park, Soo Hyun ;
Journal of Applied Biological Chemistry, volume 56, issue 2, 2013, Pages 83~87
DOI : 10.3839/jabc.2013.014
Pear (Pyrus spp.) fruit has been widely consumed fruits in the world. Pears are generally classified as Asian pears (Japanese pear: Pyruspyritolia N, China pear: P. ussuriensis M) and European pears (Pyrus communis L.). Pears have been reported to contain the diverse bioactive substances and the study on screening for bioactive compounds and its functional role in pear has been still going on. Therefore, this review article provides an overview of bioactive compounds and physiological effects of pears on diverse diseases.
Co-expression of Gamma-Aminobutyrate Aminotransferase and Succinic Semialdehyde Dehydrogenase Genes for the Enzymatic Analysis of Gamma-Aminobutyric Acid in Escherichia Coli
So, Jai-Hyun ; Lim, Yu-Mi ; Kim, Sang-Jun ; Kim, Hyun-Ho ; Rhee, In-Koo ;
Journal of Applied Biological Chemistry, volume 56, issue 2, 2013, Pages 89~93
DOI : 10.3839/jabc.2013.015
Gamma-aminobutyric acid (GABA) aminotransferase (gabT) and succinic semialdehyde dehydrogenase (gabD) genes from Pseudomonas fluorescens KCCM 12537 were cloned into a single pETDuet-1 vector and co-expressed in Escherichia coli BL21(DE3) simultaneously. The mixture of both enzymes, called GABase, is the key enzyme for the enzymatic analysis of GABA. The molecular mass of the GABA aminotransferase and succinic semialdehyde dehydrogenase were determined to be 52.8 and 46.7 kDa following computations performed with the pI/Mw program, respectively. The GABase activity between pH 6.0 and 9.0 for 24 h at
remained over 75%, but under pH 6.0 decreased rapidly. The GABase activity between 25 and
by the treatment at pH 8.6 for 30 min remained over 80%, but over
decreased rapidly. When the activity against GABA was defined as 100%, the purified GABase activity against 5-aminovaleric acid having a similar structure to GABA showed 47.7% and GABase activity against
-aspartic acid showed between 0.3 to 2.3%. The GABA content was analyzed with this co-expressed GABase, compared with the other GABase which was available commercially. As a result, the content of GABA extracted from brown rice, dark brown rice, and black rice were
, which were similar data of other GABase in the error ranges.
Korean Paddy Soil Microbial Community Analysis Method Using Denaturing Gradient Gel Electrophoresis
Choe, Myeongeun ; Hong, Sung-Jun ; Lim, Jong-Hui ; Kwak, Yunyoung ; Back, Chang-Gi ; Jung, Hee-Young ; Lee, In-Jung ; Shin, Jae-Ho ;
Journal of Applied Biological Chemistry, volume 56, issue 2, 2013, Pages 95~100
DOI : 10.3839/jabc.2013.016
Soil microbes are important integral components of soil ecosystem which have significant and diverse role in organic matter decomposition, nitrogen cycling, and nitrogen fixation. In this study an effective denaturing gradient gel electrophoresis (DGGE) method was employed for paddy soil microbial diversity survey. For optimum paddy soil microbial DNA extraction, different methods such as Lysis buffer, skim milk bead, sodium phosphate buffer, Epicentre Soil Master DNA extraction kit (Epicentre, USA) and Mo Bio Power Soil DNA kit (MO BIO, USA) methods were utilized. Among all the method, using Mo Bio Power Soil kit was most effective. DGGE analysis of Bacteria was carried out at 6% polyacylamide gel and 45-60% denaturing gradient in the optimal conditions. Whereas DGGE analysis of fungi was done at 6% polyacrylamide gel and 45-80% denaturing gradient in the optimal conditions. By applying the above assay, it was found that variation within the microbial community of paddy soil occurs by a factor of time. DGGE assay used in this study through for a variety of soil microbial analysis suggests the potential use of this method.
Effects of Turmeric (Curcuma longa L.) on the Physicochemical Characteristics of Kochujang during Fermentation
Kim, Dong-Han ;
Journal of Applied Biological Chemistry, volume 56, issue 2, 2013, Pages 101~107
DOI : 10.3839/jabc.2013.017
Physicochemical properties of kochujang prepared with different concentration of turmeric were compared. The number of yeast and bacteria in the kochujang were low in the group with more than 1% of turmeric while the amylase and protease activity increased in the group with less than 1% of turmeric. The L- and b-values increased as the concentration of turmeric increased, however a-value decreased. The titratable acidity was low in the high percentage of turmeric added group. Oxidation-reduction potential increased in the later stage of fermentation with decrease in Aw. Reducing sugar and alcohol were high in than higher turmeric added group. Amino- and ammonia-type nitrogen were the highest in the 0.5% turmeric added group. The overall acceptability of kochujang was significantly more favorable (p<0.05) in the 0.25% turmeric added group than over 1% turmeric added one.
Metabolic Differentiation of Saccharomyces cerevisiae by Ketoconazole Treatment
Keum, Young Soo ; Kim, Jeong-Han ;
Journal of Applied Biological Chemistry, volume 56, issue 2, 2013, Pages 109~112
DOI : 10.3839/jabc.2013.018
Azole fungicides are one of the most wide-spread antifungal compounds in agriculture and pharmaceutical applications. Their major mode of action is the inhibition of ergosterol biosynthesis, giving depletion of ergosterol, precursors and abnormal steroids. However, metabolic consequences of such inhibition, other than steroidal metabolitesare not well established. Comprehensive metabolic profiles of Saccharomyces cerevisiae has been presented in this study. Wild type yeast was treated either with glucose as control or azole fungicide (ketoconazole). Both polar metabolites and lipids were analyzed with gas chromatography-mass spectrometry. Approximately over 180 metabolites were characterized, among which 18 of them were accumulated or depleted by fungicide treatment. Steroid profile gives the most prominent differences, including the accumulation of lanosterol and the depletion of zymosterol and ergosterol. However, the polar metabolite profile was also highly different in pesticide treatment. The concentration of proline and its precursors, glutamate and ornithine were markedly reduced by ketoconazole. Lysine and glycine level was also decreased while the concentrations of serine and homoserine were increased. The overall metabolic profile indicates that azole fungicide treatment induces the depletion of many polar metabolites, which are important in stress response.
Investigation on Biogenic Amines in Plant-based Minor Korean Fermented Foods
Kim, Jin Hyo ; Ryu, Sung-Ji ; Lee, Ji-Won ; Kim, Young-Wan ; Hwang, Han-Joon ; Kwon, Oh-Kyoung ;
Journal of Applied Biological Chemistry, volume 56, issue 2, 2013, Pages 113~117
DOI : 10.3839/jabc.2013.019
Ten major residual biogenic amines including toxic histamine and tyramine were investigated in the plant-based minor Korean fermented food. From the analyses of pickled vegetables, fermented vegetable extracts, fermented tea, black garlic and herbal rice wines, more than 100 mg/kg of histamine were found in pickled soy leaf and pickled mulberry leaf, and also over 1,000 mg/kg of total biogenic amines were found in pickled soy leaf. No sample was found over in black garlic, fermented tea, fermented vegetable extracts and herbal rice wine, less than 100 mg/kg of histamine and/or 1,000 mg/kg of total biogenic amines were observed. Interestingly, all the tested rice wines were found to be over 100 mg/kg of agmatine residue.
Medium optimization for keratinase production by a local Streptomyces sp. NRC 13S under solid state fermentation
Shata, Hoda Mohamed Abdel Halim ; Farid, Mohamed Abdel Fattah ;
Journal of Applied Biological Chemistry, volume 56, issue 2, 2013, Pages 119~129
DOI : 10.3839/jabc.2013.020
Thirteen different Streptomyces isolates were evaluated for their ability to produce keratinase using chicken feather as a sole carbon and nitrogen sources under solid state fermentation (SSF). Streptomyces sp. NRC 13S produced the highest keratinase activity [1,792 U/g fermented substrate (fs)]. The phenotypic characterization and analysis of 16S rDNA sequencing of the isolate were studied. Optimization of SSF medium for keratinase production by the local isolate, Streptomyces sp. NRC13S, was carried out using the one-variable-at-a-time and the statistical approaches. In the first optimization step, the effect of incubation period, initial moisture content, initial pH value of the fermentation medium, and supplementation of some agro-industrial by-products on keratinase production were evaluated. The strain produced about 2,310 U/gfs when it grew on chicken feather with moisture content of 75% (w/w), feather: fodder yeast ratio of 70:30 (w/w), and initial pH 7 using phosphate buffer after 8 days. Based on these results, the Box-Behnken design and response surface methodology were applied to find out the optimal conditions for the enzyme production. The corresponding maximal production of keratinase was about 2,569.38 U/gfs.