Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Applied Biological Chemistry
Journal Basic Information
Journal DOI :
The Korean Society for Applied Biological Chemisty
Editor in Chief :
Volume & Issues
Volume 56, Issue 4 - Dec 2013
Volume 56, Issue 3 - Sep 2013
Volume 56, Issue 2 - Jun 2013
Volume 56, Issue 1 - Mar 2013
Selecting the target year
Micronucleus Test for the Classification of Chemical Mutagenicity according to Globally Harmonized System
Rim, Kyung-Taek ; Kim, Hyeon-Yeong ; Chung, Yong-Hyun ;
Journal of Applied Biological Chemistry, volume 56, issue 4, 2013, Pages 191~197
DOI : 10.3839/jabc.2013.031
To classify the chemical hazard according to globally harmonized system of classification and labeling of chemicals (GHS), we investigated the genotoxicity of three chemicals, methyl myristate, 2-ethylhexanoic acid zinc salt, N,N,N',N'-tetrakis(2-hydroxyethyl) ethylenediamine, using male ICR mice bone marrow cells for the screening of micronucleus induction. Although these three chemicals have already been tested numerous times, a micronucleus test has not been conducted. The seven week-old male ICR mice were tested at three dosages for the three chemicals, respectively. After 24 h of oral administration with the three chemicals, the mice were sacrificed and their bone marrow cells were prepared for smearing slides. As a result of counting the micronucleated polychromatic erythrocyte (MNPCE) of 2,000 polychromatic erythrocytes, all treated groups expressed no statistically significant increase of MNPCE compared to the negative control group. There were no clinical signs related with the oral exposure of these three chemicals. It was concluded that these three chemicals did not induce micronucleus in the bone marrow cells of ICR mice, and there was no direct proportion with dosage. These results indicate that the three chemicals have no mutagenic potential under each test condition, and it is not classified these chemicals as mutagens by GHS.
Antifungal, Nematicidal and Antioxidant Activity of the Methanol Extracts Obtained from Medicinal Plants
Nguyen, Dang Minh Chanh ; Seo, Dong-Jun ; Park, Ro-Dong ; Jung, Woo-Jin ;
Journal of Applied Biological Chemistry, volume 56, issue 4, 2013, Pages 199~204
DOI : 10.3839/jabc.2013.032
The nematicidal, antifungal and antioxidant activities of methanol extracts from six Vietnamese native medicinal plants were evaluated by various assays in vitro. Of the plant extracts tested, Terminalia nigrovenulosa was found to possess the highest activity when compared to the others. The leaves and bark of T. nigrovenulosa showed strong inhibitory activity against Meloidogyne incognita and Fusarium solani. The DPPH (1,1-Diphenyl-2-picryl-hydrazyl) radical scavenging, reducing power and total antioxidant activities of T. nigrovenulosa bark were higher than that of the remainder plant extracts. Chitinase activity of these plants was also investigated using SDS-PAGE. The results obtained in the present study indicate that T. nigrovenulosa leaf extracts are the greatest potential source as nematicides and fungicides for the control of M. incognita and F. solani. In particularly, T. nigrovenulosa bark extracts could be used as a potential source of commercially viable levels of natural antioxidant.
Risk Assessment on Nitrate and Nitrite in Vegetables Available in Korean Diet
Suh, Junghyuck ; Paek, Ock Jin ; Kang, YoungWoon ; Ahn, Ji Eun ; Jung, Ji Sun ; An, Yeong Soon ; Park, Sun-Hee ; Lee, Sang-Jae ; Lee, Kwang-Ho ;
Journal of Applied Biological Chemistry, volume 56, issue 4, 2013, Pages 205~211
DOI : 10.3839/jabc.2013.033
Nitrate is a naturally occurring compound in plant and can be converted to nitrite in the nitrogen cycle and vice versa. Therefore, it is easy to find nitrate in plants including vegetables. Especially, it is known that high levels of nitrate found in leafy vegetables. Nitrate itself is relatively non-toxic but its metabolites and reaction products such as nitrite, nitric oxide and N-nitroso compounds, may produce adverse health effects such as methaemoglobinaemia and carcinogenesis. To execute the risk assessment of dietary nitrate and nitrite for the intake of vegetables, it is investigated that the levels of nitrate and nitrite in 23 vegetables (798 samples). The range of concentration were 0-6,719mg/kg for nitrate and 0-1,635mg/kg for nitrite, respectively. The Estimated daily intakes (EDI) were 0.85-1.38 mg/kg body weight/day for nitrate and 0.02-0.03 mg/kg body weight/day for nitrite by ages. We conclude that there are no health concerns for eating various vegetables since the EDI were below the Tolerable Daily Intake (3.7 mg/kg body weight/day for nitrate, 0.06 mg/kg body weight/day for nitrite) level established by WHO.
Flavonoids from the Leaves of Ailanthus altissima Swingle and their Antioxidant Activity
Lee, Min-Kyung ; Kim, Su-Yeon ; Park, Ji-Hae ; Lee, Do-Gyeong ; Lee, Dae-Young ; Kim, Geum-Soog ; Kim, Yong-Bum ; Han, Dae-Seok ; Lee, Chang-Ho ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 56, issue 4, 2013, Pages 213~217
DOI : 10.3839/jabc.2013.034
Phytochemical studies on the leaves of Ailanthus altissima (Simaroubaceae) have not been reported previously. Thus, the authors isolated and identified secondary metabolites from A. altissima. Dried and powdered leaves were extracted with 80% aqueous methanol, and the concentrated extract was successively partitioned with ethyl acetate, n-butanol, and water. Four flavonoids were isolated from the ethyl acetate fraction through repeated silica gel and octadecyl silica gel column chromatography. Spectroscopic data including NMR, MS, and IR allowed for identification of the chemical structures as quercetin (1), afzelin (2), quercitrin (3), and isoquercitrin (4). This is the first report of the isolation of these compounds from A. altissima. The four isolated flavonoids 1-4 as well as solvent fractions (ethyl acetate, n-butanol, and water), were evaluated for DPPH radical scavenging activity.
Effect of Cheonggukjang Made from Sword Bean on Serum Lipid Profile and Tissue Change in Rats
Kim, Jae-Young ; Kim, Un-Sung ; Chang, Moon-Ik ; Moon, Kwang-Hyun ; Baek, Seung-Hwa ;
Journal of Applied Biological Chemistry, volume 56, issue 4, 2013, Pages 219~227
DOI : 10.3839/jabc.2013.035
This study was to investigate serum triglyceride (TG), phospholipid (PL), and cholesterol concentrations after feeding the combined diets of prepared traditional Cheonggukjang (TC) or Cheonggukjang made from sword bean powder (CS), the feed, and high-fat cholesterols at certain ratio for 8 weeks in rats, and then confirmed the effects of the diets based on the changes in the tissues of livers, kidneys, and spleens. The serum TG concentrations were lower in CS group compared to that of in TC group. The increase of PL concentrations was greater in CS group than that of TC group. FChCS6 group (92% feed+2% cholesterol+6% CS) showed the lowest total-cholesterol (T-C),
, indicating that CS was effective to lower the T-C. Both TG/PL and T-C/PL ratio tended to be decreased. As CS intake was effective in the reduction of low density lipoprotein-cholesterol level as well as the increase of high density lipoprotein-cholesterol level, it would be effective in the prevention of adult diseases and circulatory diseases. It was confirmed that there were no changes in organ appearance and tissues in the rats with CS intake. Therefore, CS intake showed superior functionalities than that of TC intake.
Effect of Fly Ash Fertilizer on Paddy Soil Quality and Rice Growth
Oh, Se Jin ; Yun, Hyun Soo ; Oh, Seung Min ; Kim, Sung Chul ; Kim, Rog Young ; Seo, Yung Ho ; Lee, Kee Suk ; Ok, Yong Sik ; Yang, Jae E. ;
Journal of Applied Biological Chemistry, volume 56, issue 4, 2013, Pages 229~234
DOI : 10.3839/jabc.2013.036
Coal ash can be added to agricultural soils to increase the chemical properties of soil such as pH, cation exchange capacity and nutrient availability of - B, Ca, Mo etc-. Therefore, the main purpose of this study was to evaluate the feasibility of fly ash as a soil amendment in paddy soils. Selected fly ash was mixed with bentonite and calcium hydroxide at the ratio of 80:15:5 (w/w) and manufactured as a pellet type at the size of 10 mm. Field experiments were conducted to evaluate the effects of fly ash fertilizer on the soil quality and crop growth compare to the control (no fertilizer) and, - traditional fertilizer. Results showed that soil pH and organic matter in paddy soils after applying the manufactured fly ash fertilizer were not increased compared to the other two treatments. However, the concentration of available phosphate and silicate in paddy soils were higher than those of the control and traditional fertilization. With regard to crop growth, no significant difference was observed between three different treatments. However, the content of protein in the rice grain cultivated with the fly ash fertilizer was higher than in the rice cultivated by other two treatments. Overall, fly ash fertilizer could increase the concentration of available silicate and phosphate in the paddy soil and improve the rice quality. In conclusion, fly ash can be utilized in agricultural soils as soil amendment, especially in the rice paddy soil.
Hairy Vetch Growth-Promoting Rhizobium sp. RH84 and Application to Reclaimed Land
Jang, Jong-Ok ; Kwon, Mi-Kyung ; Park, Dong-Jin ; Sung, Chang Keun ; Kim, Chang-Jin ;
Journal of Applied Biological Chemistry, volume 56, issue 4, 2013, Pages 235~239
DOI : 10.3839/jabc.2013.037
This study was carried out to investigate the plant growth promoting activity of hairy vetch (Vicia villosa Roth) on reclaimed land. At the previous research, Rhizobium sp. RH84 was isolated and selected for further study from hairy vetch. For the investigation of plant growth promoting effects by the Rhizobium sp. RH84, production of indole acetic acid (IAA), siderophore, phosphate solubilization and nitrogen fixation were tested and other characters were examined. As results, RH84 produced
IAA per mL and showed nitrogen fixation activity. With the treatment of Rhizobium sp. RH84 to hairy vetch showed good growth at 0.3% salty reclaimed soil, and the production yield was increased up to 56% at field test. From these results, it was confirmed that the Rhizobium sp. RH84 would be used as a green manure for hairy vetch under the salty condition of reclaimed land.
Characterization of Microbial Nitrate Uptake by Bacillus sp. PCE3
Yun, Yeong-Bae ; Park, Soo-Jin ; Han, Min-Woo ; Kim, Young-Kee ;
Journal of Applied Biological Chemistry, volume 56, issue 4, 2013, Pages 241~244
DOI : 10.3839/jabc.2013.038
Nitrate is one of the major nutrients in plants, and nitrate fertilizer often overused for the high yields of crops. Nitrate deposit in soil became one of the major reasons causing salt stress. Specially, salt stress is a serious problem in the soils of plastic film or glass houses. In this study, six microorganisms have been isolated from the wet soils near the disposals of livestock farms and their nitrate uptake activities were investigated. These bacteria were able to remove nitrate as high as 1,000-3,000 ppm (10-50 mM). The strain PCE3 showed the highest nitrate uptake activity and it removed more than 3,700 ppm. In order to identify these bacteria, genes of 16S rRNA were sequenced and analyzed. Phylogenetic trees were constructed with the neighbor-joining methods. Among these bacteria, strain PCE3 was identified as Bacillus species. When the growth and nitrate uptake activities were measured, both were maximal at
and optimal pH was pH 7-9. Bacillus sp. PCE3 removed nitrate up to 40-60 mM (2,500-3,700 ppm) depending on the nitrate concentration in media. Therefore, Bacillus sp. PCE3 can be a good candidate for the microbial remediation of nitrate-deposited soils in glass and plastic film houses.
Potential of Red Ginseng Marc for Ethanol Production as a Fermentation Medium
Kim, Dong Chung ; In, Man-Jin ;
Journal of Applied Biological Chemistry, volume 56, issue 4, 2013, Pages 245~247
DOI : 10.3839/jabc.2013.039
The potential of the red ginseng marc (RGM) for the production of bio-ethanol using enzymatic hydrolysis and fermentation without any additional nutrients was investigated. Reducing sugar content in RGM treated with Viscozyme and Flavourzyme was 101.1 g/L and was much higher than that (7.2 g/L) in intact RGM. When enzymatically hydrolyzed red ginseng marc (ERGM) was fermented with commercially available dry yeast at
for 7 days, the final ethanol concentration reached 29.3 g/L with ethanol yield at 0.274 g of ethanol per 1 g of solubilized total sugar. Ethanol concentration and ethanol yield of ERGM were drastically increased over 1000% and 50%, respectively than those of RGM.
Inhibitory Effect of Sargauum fulvellum Ethanolic Extract on LPS-Induced Inflammatory Reaction in RAW 264.7 Mouse Macrophages
Kim, Min-Ji ; Kim, Koth-Bong-Woo-Ri ; Jeong, Da-Hyun ; Ahn, Dong-Hyun ;
Journal of Applied Biological Chemistry, volume 56, issue 4, 2013, Pages 249~255
DOI : 10.3839/jabc.2013.040
Recently, algae has been considered as a potential anti-inflammatory source due to its distinctive habitat environment exposing to light and high oxygen concentration. In present study, anti-inflammatory effect of brown alga, Sargassum fullvellum ethanol extract (SFEE), was examined. SFEE inhibited not only the production of nitric oxide and pro-inflammatory cytokines (IL-6, IL-
) but also the expression of inducible nitric oxide synthase and cyclooxygenase 2 in LPS-induced RAW 264.7 cells without affecting cell viability. SFEE also suppressed the expression of nuclear factor kappa B (NF-
), suggesting that SFEE could affect the expression of inflammation related cytokines and proteins through the regulation of NF-
. Furthermore, formation of edema of the ear was 40% lower in mice treated with the highest dose (250 mg/kg) of SFEE than in the control mice. Thus, our study showed that SFEE may be a potential therapeutic anti-inflammatory drug.
Erratum to: Cytotoxicity of Neolignans from Magnolia obovata Fruits
Seo, Kyeong-Hwa ; Lee, Dae-Young ; Jeong, Rak-Hun ; Yoo, Ki-Hyun ; Chung, In-Sik ; Kim, Geum-Soog ; Seo, Woo-Duck ; Kang, Hee-Cheol ; Ahn, Eun-Mi ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 56, issue 4, 2013, Pages 257~257
DOI : 10.3839/jabc.2013.041